Abstract Background: Hematoporphyrin derivative (HPD) has a sensibilization effect in lung adenocarcinoma. This study was conducted to identify the target genes of HPD in lung adenocarcinoma. Methods: RNA sequencing was performed using the lung adenocarcinoma cell line A549 after no treatment or treatment with X-ray or X-ray + HPD. The differentially expressed genes (DEGs) were screened using Mfuzz package by noise-robust soft clustering analysis. Enrichment analysis was carried out using “BioCloud” online tool. Protein–protein interaction (PPI) network and module analyses were performed using Cytoscape software. Using WebGestalt tool and integrated transcription factor platform (ITFP), microRNA target and transcription factor (TF) target pairs were separately predicted. An integrated regulatory network was visualized with Cytoscape software. Results: A total of 815 DEGs in the gene set G1 (continuously dysregulated genes along with changes in processing conditions [untreated—treated with X-ray—X-ray + treated with HPD]) and 464 DEGs in the gene set G2 (significantly dysregulated between X-ray + HPD-treated group and untreated/X-ray-treated group) were screened. The significant module identified from the PPI network for gene set G1 showed that ribosomal protein L3 (RPL3) gene could interact with heat shock protein 90 kDa alpha, class A member 1 (HSP90AA1). TFs AAA domain containing 2 (ATAD2) and protein inhibitor of activated STAT 1 (PIAS1) were separately predicted for the genes in gene set G1 and G2, respectively. In the integrated network for gene set G2, ubiquitin-specific peptidase 25 (USP25) was targeted by miR-200b, miR-200c, and miR-429. Conclusion: RPL3, HSP90AA1, ATAD2, and PIAS1 as well as USP25, which is targeted by miR-200b, miR-200c, and miR-429, may be the potential targets of HPD in lung adenocarcinoma.

Abstract: The “scatter degree” method is an objective evaluation method based on a “difference drive*”. However, it is currently perceived as incapable of properly reflecting the subjective information of the evaluator. In order to reflect the objective law of raw data and assist decision-makers to control major factors of the water environmental carrying capacity (WECC), this paper proposes a method to process the weight of the indicator by combining the analytic hierarchy process (AHP) and the vertical “scatter degree” method. The computing results of this improved method are virtually identical to those of the old method, but the former is more scientific and reliable.

Resumen: El método de “grado de dispersión” vertical es un método de evaluación objetiva; sin embargo, actualmente se percibe como incapaz de reflejar de forma adecuada la información subjetiva del evaluador. Con el fin de reflejar la ley objetiva de datos brutos y ayudar a los tomadores de decisiones a controlar los principales factores de la capacidad de carga ecológica del agua (WECC), este trabajo propone un método para procesar la ponderación del indicador al combinar el proceso de jerarquía analítica (AHP) y el método de “grado de dispersión” vertical. Los resultados de este método mejorado son prácticamente idénticos a los obtenidos con el antiguo método, pero el primero es más científico y confiable.

Chitosan-Ru2(bpy)32+-SiO2 composite nanoparticles (CRuS NPs) were prepared by reverse microemulsion method for the electronic attraction between negative charge of hydroxyl groups in chitosan and positive charge in Ru2(bpy)3 2+. Based on the multi-walled carbon nanotubes (MCNT)/Nafion composite membrane technology, CRuS NPs was effectively and steadily immobilized on the surface of a glassy carbon electrode. The CRuS NPs synthesized were characterized by transmission electron microscope (TEM), UV-Vis spectroscopy and fluorescence spectroscopy, while the modification was confirmed by cyclic voltammetry and electrogenerated chemiluminescence (ECL). The ECL studies revealed that the sensor had a good response to different concentrations of tiapride hydrochloride and the increased ECL intensity was directly related to the logarithm of tiapride hydrochloride concentrations in the range from 0.01 to 3.0 µmol L-1 with a detection limit of 6.0 nmol L-1. The sensor was successfully applied to the determination of tiapride hydrochloride in pharmaceutical.

In this work, we report the cloning and characterization of endo-β-1,4-glucanase (EGase) genes (TaEG) in the common wheat line three pistils. Three TaEG homoeologous genes (TaEG-4A, TaEG-4B and TaEG-4D) were isolated and found to be located on chromosomes 4AL, 4BS and 4DS, respectively. The three genes showed high conservation of their coding nucleotide sequences and 3 untranslated region. The putative TaEG protein had a molecular mass of 69 kDa, a theoretical pI of 9.39 and a transmembrane domain of 74-96 amino acids in the N-terminus that anchored the protein to the membrane. The genome sequences of TaEG-4A, TaEG-4B and TaEG-4D contained six exons and five introns. All of the introns, except for intron IV, varied in length and sequence composition. Phylogenetic analysis revealed that TaEG was most closely related to rice (Oryza sativa) OsGLU1. The TaEG transcript levels increased significantly during the subsidiary pistil primordium differentiation phase (spike size ~7-10 mm) in Chuanmai 28 TP (CM28TP). These data provide a basis for future research into the function of TaEG and offer insights into the molecular mechanism of the three pistils mutation in wheat.