Severe blockage in myeloid differentiation is the hallmark of acute myeloid leukemia (AML). Trdmt1 plays an important role in hematopoiesis. However, little is known about the function of Trdmt1 in AML cell differentiation. In the present study, Trdmt1 was up-regulated and miR-181a was down-regulated significantly during human leukemia HL-60 cell differentiation after TAT-CT3 fusion protein treatment. Accordingly, miR-181a overexpression in HL-60 cells inhibited granulocytic maturation. In addition, our “rescue” assay demonstrated that Trdmt1 3′-untranslated region promoted myeloid differentiation of HL-60 cells by sequestering miR-181a and up-regulating C/EBPα (a critical factor for normal myelopoiesis) via its competing endogenous RNA (ceRNA) activity on miR-181a. These findings revealed an unrecognized role of Trdmt1 as a potential ceRNA for therapeutic targets in AML.

RESUMEN Este estudio estableció el modelo matemático de filtración de agua en una capa carbonífera al estimar la salida acuífera con el uso del coeficiente de fuga, de acuerdo con la teoría de filtración inestable, para analizar los efectos en la recarga de pérdida de fluidos de un acuífero en el drenado inicial para pozos de gas metano. El modelo se resolvió tras usar la transformación Laplace y la inversión numérica Stehfest para encontrar la respuesta en el lugar indicado. Luego, se creó la representación algorítmica de la presión y la presión derivativa con nuevas combinaciones de parámetros. Se evaluó la influencia de la pérdida de fluido del acuífero en la forma de la curva con base al mecanismo físico de filtración. Se estableció que el flujo radial finaliza antes de que el coeficiente de pérdida de fluido se incremente. Además, se propone el uso de la curva tipo correspondiente para obtener la permeabilidad del reservorio, el factor de daño y el coeficiente de pérdida de fluido. Las curvas tipo son útiles para evaluar cuantitativamente el nivel de la pérdida de fluido, y de esta manera guiar el ajuste de un sistema de producción consecuente para pozos de gas metano de carbón.

ABSTRACT To analyze the effects of the leakage recharge of the aquifer on the initial dewatering of coalbed methane wells, the mathematical seepage model of water in the coalbed considering the aquifer leakage was established by using the leakage coefficient according to the unsteady seepage theory. The model was solved after Laplace transform and the Stehfest numerical reverse inversion was used to obtain the solution in right space. Then, the log-log type curves of pressure and pressure derivative were created with new combinations of parameters. Based on the natural seepage mechanism, the influence of aquifer leakage on curve shape was judged. It is found that the radial flow ends earlier as the leakage coefficient increases. Moreover, it was proposed to obtain reservoir permeability, skin factor, and leakage coefficient by using type curve matching. The type curves are useful for quantitatively evaluating the level of leakage, thereby guiding the adjustment of the following production system for CBM wells.

RESUMEN Las reservas de gas metano de carbón (CBM, del inglés Coalbed Methane) en la cuenca Qinshui son más que prometedoras, pero la producción en los pozos es muy pequeña, incluso después de fracturas hidráulicas masivas. En este trabajo se analizaron los sistemas de fractura de las vetas de carbón #3 y #15 de la cuenca Qinshui y se encontró que tanto las fracturas a macroescala como aquellas a microescala están cubiertas con arcillas y minerales carbonatos, lo que explica la baja productividad de los pozos de gas metano de carbón después de la fractura hidráulica convencional. La fractura ácida ha sido un método efectivo en los depósitos de gas carbonato para mejorar la producción en el pozo de gas. Sin embargo, existen pocos informes sobre la aplicación de la fractura ácida en el campo del gas metano de carbón. De acuerdo con la identificación mineral y las pruebas de sensibilidad ácida, la factibilidad de la fractura ácida demostró que el ácido es reparador en el incremento de la permeabilidad en las vetas de carbón de la cuenca Qinshui. Las operaciones in situ han demostrado que la fractura ácida es aplicable para los pozos de gas metano de carbón en el área minera de Jincheng. También se observó en el sondeo microsísmico que cuando se aplica un tratamiento de fractura ácida, el área del depósito estimulada depende del volumen de ácido bombeado en primera instancia, lo que es determinante en el éxito de la estimulación.

ABSTRACT The reserves of Coalbed Methane (CBM) in Qinshui Basin are quite promising, but the outputs from CBM wells are quite small even after massive hydraulic fracturing. Herein the fracture system with #3 and #15 coal seams in Qinshui basin was analyzed, and it was found that both of the macro-scale fractures and micro-scale fractures are filled with clay and carbonate minerals, which explains the low productivity of CBM wells after conventional hydraulic fracturing. Acid fracturing has long been an effective method for carbonate gas reservoir to improve the gas well production. However, there were few reports about the application of acid fracturing in coal bed methane field. Based on the mineral identification and acid sensitivity test, the feasibility of acid fracturing demonstrated that the acid does more help than damage to increase the permeability of coal seams in Qinshui basin. Onsite operations have shown that acid fracturing is applicable for the CBM wells in Jincheng Mining Area. It was also observed from the microseismic survey that when applying the acid fracturing treatment, the stimulated reservoir area depends on the acid volume pumped in the first stage, which is crucial to the success of the stimulation.

Abstract Zea mays L. has been the most cultivated crop and the crop with the largest yield in China since 2012. We constructed a bacterial artificial chromosome (BAC) library for the maize inbred line Qi319, which may be used as a key source for disease-resistant maize breeding in China. The BAC contains 270,720 clones, with an average insert size of 90 kb. The coverage of the library is about 10.43 genome equivalents when considering a haploid genome size of 2300 Mb, providing a 99.99% likelihood of isolating any maize gene or sequence in the library. An average of 12 clones were obtained by polymerase chain reaction screening by using primer pairs linked to the genes for resistance to maize southern rust and rough dwarf. The results indicate that the library can satisfy the requirements for recovering specific sequences. The library is available to researchers to whom it may be of interest.

The aim of this work was to study the genetic diversity among flue-cured tobacco cultivars. RAPD and AFLP analyses were used to assess the genetic similarity among selected accessions of flue-cured tobacco. Seventy eight RAPD and 154 AFLP polymorphic bands were obtained and used to assess the genetic diversity among 28 tobacco accessions. The cultivar relationships were estimated through the cluster analysis (UPGMA) based on RAPD data and AFLP data. The accessions were grouped into three major clusters and these shared common ancestry clustered together.

This study presents bacterial population analyses of microbial communities inhabiting three sites of acid mine drainage (AMD) in the Shen-bu copper mine, Gansu Province, China. These sites were located next to acid-leached chalcopyrite slagheaps that had been abandoned since 1995. The pH values of these samples with high concentrations of metals ranged from 2.0 to 3.5. Amplified ribosomal DNA restriction analysis (ARDRA) was used to characterize the bacterial population by amplifying the 16S rRNA gene of microorganisms. A total of 39 operational taxonomic units (OTUs) were obtained from the three samples and sequenced from 384 clones. Sequence data and phylogenetic analyses showed that two dominant clones (JYC-1B, JYC-1D) in sample JYC-1 represented 69.5% of the total clones affiliated with Acidithiobacillus ferrooxidans (γ-Proteobacteria), and the most dominant clones of JYC-2 and JYC-3 were affiliated with Caulobacter crescentus (α-Protebacteria). At the level of bacterial divisions, differences in the relative incidence of particular phylogenetic groups among the three samples and discrepancies in physicochemical characteristics suggested that the physico-chemical characteristics had an influence on phylogenetic diversity. Furthermore, the relationships between the discrepancies of physicochemical characteristics and the diversity of the bacteria communities in the three samples suggested that the biogeochemical properties, pH and concentration of soluble metal, could be key factors in controlling the structure of the bacterial population