ABSTRACT Purpose: To investigate putative mechanism of wound healing for chitosan-based bisacurone gel against secondary burn wounds in rats. Methods: A second-degree burn wound with an open flame using mixed fuel (2 mL, 20 seconds) was induced in Sprague Dawley rats (male, 180-220 g, n = 15, each) followed by topical treatments with either vehicle control (white petroleum gel, 1%), silver sulfadiazine (1%) or bisacurone gel (2.5, 5, or 10%) for 20 days. Wound contraction rate and paw withdrawal threshold were monitored on various days. Oxidative stress (superoxide dismutase, glutathione, malondialdehyde, and nitric oxide), pro-inflammatory cytokines (tumour necrosis factor-alpha, interleukins by enzyme-linked immunosorbent assay), growth factors (transforming growth factor-β, vascular endothelial growth factor C using real time polymerase chain reaction and Western blot assay) levels, and histology of wound skin were assessed at the end. Results: Bisacurone gel showed 98.72% drug release with a 420.90–442.70 cps viscosity. Bisacurone gel (5 and 10%) significantly (p < 0.05) improved wound contraction rate and paw withdrawal threshold. Bisacurone gel attenuated oxidative stress, pro-inflammatory cytokines, and water content. It also enhanced angiogenesis (hydroxyproline and growth factor) and granulation in wound tissue than vehicle control. Conclusions: These findings suggested that bisacurone gel can be a potential candidate to treat burn wounds via its anti-inflammatory, antioxidant, and angiogenic properties. Purpose chitosanbased chitosan based Methods seconddegree second degree 2 ( mL seconds male, male (male 180220 180 220 180-22 g 15 each white 1%, 1 1% , 1%) (1% 2.5, 25 5 (2.5 10% 10 days superoxide dismutase glutathione malondialdehyde oxide, oxide oxide) proinflammatory pro inflammatory tumour factoralpha, factoralpha alpha, alpha factor-alpha enzymelinked enzyme linked assay, assay transforming factorβ, factorβ β, β factor-β levels end Results 9872 98 72 98.72 4209044270 420 90 442 70 420.90–442.7 viscosity p 0.05 005 0 05 content hydroxyproline Conclusions antiinflammatory, antiinflammatory anti inflammatory, anti-inflammatory antioxidant properties 18022 18 22 180-2 (1 2.5 (2. 987 9 7 98.7 420904427 42 44 420.90–442. 0.0 00 1802 180- 2. 98. 42090442 4 420.90–442 0. 4209044 420.90–44 420904 420.90–4 42090 420.90– 4209 420.90 420.9 420.