Abstract The antibacterial secondary metabolites of the fungus Penicillium chrysogenum associated with the beetle Aspongopus chinensis were investigated through chromatographic fractionation methods of ethyl acetate extracts of the fungal cultures. Five compounds were isolated, and their structures were determined as emodin, 4-(methoxymethyl)benzoic acid, isoochracinic acid, secalonic acid D, and dicerandrol A using mass spectroscopy and nuclear magnetic resonance spectroscopic analyses. Emodin exhibited strong antimicrobial activity, especially against Staphylococcus aureus even when growing on cooked pork, with a minimal inhibitory concentration (MIC) of 6.3 μg/mL. Dimeric tetrahydroxanthones, such as secalonic acid D and dicerandrol A, also exhibited potent activity, with MIC values ranging from 9.5 to 28.5 μg/mL. In summary, P. chrysogenum was isolated as a symbiotic fungus of the beetle A. chinensis for the first time and this strain could generate antibacterial secondary metabolites, which could potently inhibit gram-positive bacteria growth in vitro. cultures emodin 4methoxymethylbenzoic methoxymethylbenzoic 4 methoxymethyl benzoic analyses activity pork (MIC 63 6 3 6. μgmL μg mL μg/mL tetrahydroxanthones 95 9 5 9. 285 28 28. summary P grampositive gram positive vitro 2

Abstract In this study, type II collagen was used to induce arthritis in mice to test the effect and mechanism of dandelion tea. The visual manifestations of arthritis, histopathological conditions of spleen, serum inflammatory factors and gene expression of synovium of foot and ankle joints in mice were detected by the methods of arthritis index (AI), spleen index, enzyme linked immunosorbent assay (ELISA), hematoxylin-eosin (H&E) staining and quantitative polymerase chain reaction (qPCR). The compound components of dandelion tea were determined by high performance liquid chromatography (HPLC). The experimental results show that dandelion tea can reduce the AI value, spleen index and paw swelling degree of arthritis mice, reduce the pathological damage of spleen, and increase the serum IL-10 and TGF-β levels, and can up-regulate Foxp3 expression and down regulate ROR-γt expression in the synovium of ankle joint. At the same time, the results of component analysis showed that the efficacy of dandelion tea came from the contents of gallic acid, protocatechuic acid, chlorogenic acid, caffeic acid, p-coumaric acid, rutin, myricetin, isoquercitrin, isochlorogenic acid and luteolin. It can be seen that dandelion tea is a health drink with good intervention effect on arthritis.

Abstract Background: Retinal neurodegeneration is induced by a variety of environmental insults and stresses, but the exact mechanisms are unclear. In the present study, we explored the involvement of cytosolic mitochondrial DNA (mtDNA), resulting in the cGAS-STING dependent inflammatory response and apoptosis in retinal damage in vivo. Methods: Retinal injury was induced with white light or intravitreal injection of lipopolysaccharide (LPS). After light-or LPS-induced injury, the amount of cytosolic mtDNA in the retina was detected by PCR. The mtDNA was isolated and used to transfect retinas in vivo. WB and real-time PCR were used to evaluate the activation of cGAS-STING path-way and the levels of apoptosis-associated protein at different times after mtDNA injection. Retinal cell apoptosis rate was detected by TUNEL staining. Full-field electroretinography (ERG) was used to assess the retinal function. Results: Light injury and the intravitreal injection of LPS both caused the leakage of mtDNA into the cytoplasm in retinal tissue. After the transfection of mtDNA in vivo, the levels of cGAS, STING, and IFN-β mRNAs and the protein levels of STING, phosph-TBK1, phospho-IRF3, and IFN-β were upregulated. mtDNA injection also induced the activation of caspase 3 and caspase 9. BAX and BAK were increased at both the mRNA and protein levels. The release of cytochrome c from the mitochondria to the cytosol was increased after mtDNA injection. The wave amplitudes on ERG decreased and retinal cell apoptosis was detected after mtDNA injection. Conclusions: Cytosolic mtDNA triggers an inflammatory response. It also promotes apoptosis and the dysfunction of the retina.

ABSTRACT Fulvic acid (FA) and superabsorbent polymer (SAP) are widely applied to improve crop growth and yield under water deficit conditions, but little is known about the changes in crop physiological parameters related to water deficit tolerance when SAP and FA are combined. A pot test with maize (Zea mays L.) plants was conducted to examine the combined effect on photosynthesis, leaf water, proline, and growth under soil water deficit. Maize plants were subjected to two soil moisture conditions at the late crop growth phase: water deficit (WD, 50% field capacity) and well-watered (WW, 80% field capacity). The SAP (4.5 g m-2) was mixed into the soil layer at sowing and the FA solution (2 g L-1) was sprayed twice during water control. The combined application significantly improved maize grain yield under both watering conditions. The net photosynthesis rate, intrinsic quantum yield, fluorescent parameter (Fv/Fm), and chlorophyll content all improved with the combined application under both watering regimes. The compensating effect of combining chemicals on yield and photosynthesis parameters was higher than when applied alone under the two watering conditions. For prolonged and WD conditions, leaf proline and water content were higher under the combined treatment than when used separately. Under the WD conditions treated with FA and SAP, Fv/Fm had positive significant correlations with leaf water content and osmotic potential; leaf proline did not show any correlations with either the osmotic potential or leaf water content. This result demonstrated that SAP and FA could be combined to maintain high leaf proline and improve photosynthesis to mitigate adverse effects of moderate water storage on maize growth