Abstract Objective Early diagnosis of Severity Mycoplasma Pneumoniae Pneumonia (SMPP) has been a worldwide concern in clinical practice. Two cytokines, soluble Triggering Receptor Expressed on Myeloid cells (sTREM-1) and Interferon-Inducible Protein-10 (IP-10), were proved to be implicated in bacterial infection diseases. However, the diagnostic value of sTREM-1 and IP-10 in MPP was poorly known. This study aimed to investigate the diagnostic value of sTREM-1 and IP-10 for SMPP. Methods In this prospective study, the authors enrolled 44 children with MPP, along with their clinical information. Blood samples were collected, and cytokine levels of sTREM-1 and IP-10 were detected with ELISA assay. Results Serum levels of sTREM-1 and IP-10 were positively correlated with the severity of MPP. In addition, sTREM-1 and IP-10 have significant potential in the diagnosis of SMPP with an Area Under Curve (AUC) of 0.8564 (p-value = 0.0001, 95% CI 0.7461 to 0.9668) and 0.8086 (p-value = 0.0002, 95% CI 0.6918 to 0.9254) respectively. Notably, the combined diagnostic value of sTREM-1 and IP-10 is up to 0.911 in children with SMPP (p-value < 0.001, 95% CI 0.830 to 0.993). Conclusions Serum cytokine levels of sTREM-1 and IP-10 have a great potential diagnostic value in children with SMPP. (SMPP practice cytokines sTREM1 sTREM 1 (sTREM-1 InterferonInducible Interferon Inducible Protein10 Protein 10 Protein-1 IP10, IP10 IP , (IP-10) diseases However sTREM- IP-1 known 4 information collected assay addition AUC (AUC 08564 0 8564 0.856 pvalue p 00001 0001 0.0001 95 07461 7461 0.746 0.9668 09668 9668 08086 8086 0.808 00002 0002 0.0002 06918 6918 0.691 0.9254 09254 9254 respectively Notably 0911 911 0.91 001 0.001 0830 830 0.83 0.993. 0993 0.993 . 993 0.993) (sTREM- Protein1 Protein- IP1 (IP-10 IP- 0856 856 0.85 0000 000 0.000 9 0746 746 0.74 0.966 0966 966 0808 808 0.80 0691 691 0.69 0.925 0925 925 091 91 0.9 00 0.00 083 83 0.8 099 0.99 99 (sTREM (IP-1 085 85 074 74 0.7 0.96 096 96 080 80 069 69 0.6 0.92 092 92 09 0. 0.0 08 8 (IP- 07 7 06 6 (IP

Abstract Polysaccharides are a class of biological macromolecules made up of sugar chains with multiple glycosidic bonds. To explore the extracted, purified, carboxymethylated and phosphorylated modified to obtained carboxymethylated polysaccharides (HSPS) and phosphorylated polysaccharides (HSPP), and the HSPS and HSPP were characterized and analyzed, and their antioxidant activity measured. Response surface design was used for the synthesis process of HSPS and HSPP, the structure characterization and antioxidant activities of HSPS and HSPP were evaluated. The results showed that the Mw and Mn of HSP were 14.66 kDa and 8.39 kDa, respectively. The monosaccharides were mainly galactose, glucose, xylose, and arabinose in a molar ratio of 37.68%: 24.43%: 13.63%: 9.18%. The optimal synthesis conditions for HSPS were as follows: reaction time of 2.01 h, reaction temperature of 44.61°C and the amount of monochloroacetic acid necessary was 1.18 g. The optimal synthesis conditions for HSPP were: reaction time of 4.28 h, reaction temperature of 80.78°C, and reaction pH of 8.98. HSPS and HSPP have scavenging effects on ABTS, super oxideanion and hydroxyl radicals. The HSPS and HSPP were characterized and analyzed, and their antioxidant activity measured. bonds extracted purified (HSPS , (HSPP) analyzed measured evaluated 1466 14 66 14.6 839 8 39 8.3 respectively galactose glucose xylose 37.68% 3768 37 68 24.43% 2443 24 43 13.63% 1363 13 63 918 9 18 9.18% follows 201 2 01 2.0 h 4461C C 44 61 118 1 1.1 g 428 4 28 4.2 8078C 80 78 80.78°C 898 98 8.98 ABTS radicals (HSPP 146 6 14. 83 3 8. 37.68 376 24.43 244 13.63 136 91 9.18 20 0 2. 11 1. 42 4. 7 89 8.9 37.6 24.4 13.6 9.1 37. 24. 13. 9.

Abstract In this study, we extracted lotus root polysaccharide (LRP) and synthesised phosphorylated lotus root polysaccharide (PLP) using response surface methodology (RSM). RSM analyses revealed that the optimal conditions for PLP synthesis were a reaction duration of 7 h, temperature of 70 °C and pH of 11.38. Under these conditions, the predicted degree of substitution (DS%) was determined to be 9.96%. The structure of the LRP1 was examined by ultraviolet (UV) spectroscopy scan, Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (NMR) (1H and 13C). The monosaccharide composition of LRP1 was determined to be mannose (0.12%), ribose(0.18%), glucuronic acid(0.60%), galacturonic acid(0.09%), glucose(98.79%) and galactose(0.21%). The number average molecular weight (Mn) and the weight average molecular weight (Mw) of LRP1 were 10236 and 251783 g/mol. LRP1 exhibited high antioxidant activities in scavenging ABTS radicals, Superoxide anion radicals and Metal ion scavenging activity. PLP exhibited strong antioxidant activity in vitro. In addition, PLP inhibited Skov3 cancer cell proliferation and induced reactive oxygen species (ROS) production. Our data revealed that PLP is a promising natural antioxidant with potential value as a food supplement and for the treatment of cancer.