ABSTRACT Mixed infection by Histoplasma capsulatum isolates with different mating types, in AIDS-patients are described in this study. Morphological, mating type-specific PCR assay and multilocus sequencing type analysis of H. capsulatum isolates recovered from two Brazilian AIDS-patients were performed. Five H. capsulatum isolates were recovered at different times from the two patients. Three isolates were obtained from bone marrow (day 1 – CE0411) and buffy coat cultures (day 1 – CE0311; day 2 – CE0511) of patient 1, and two isolates were isolated from buffy coat cultures (day 3 – CE2813; day 12 – CE2513) of patient 2. The mycelial colonies depicted different textures and pigmentation features. Dimorphic conversion to the yeast-phase in ML-Gema medium was achieved in all isolates. MAT1-1 idiomorph was identified in CE0311, CE0411 and CE2813 isolates; MAT1-2 idiomorph was found in CE0511 and CE2513 isolates. These H. capsulatum isolates were grouped within LAm A clade, highlighting that CE0311 and CE0411 isolates formed a subgroup supported by a high bootstrap value. The CE0511, CE2513, and CE2813 isolates clustered together with a Brazilian H151 isolate. This research reports mixed infections caused by H. capsulatum isolates with different mating types in Brazilian AIDS-patients for the first time in the literature.

A taxonomic review of the specimens of the genus Helvella housed at FCME (UNAM) Herbarium was carried out; 14 species were determined. The knowledge of this genus in the states of Chihuahua, Distrito Federal, Estado de Mexico, Guanajuato, Guerrero, Michoacán, Nayarit, Oaxaca, Querétaro and Tlaxcala is widened. A sinoptical key is provided for the species cited from Mexico, as well as the morphological description of selected taxa.

Se realizó una revisión taxonómica de los ejemplares del género Helvella depositados en el Herbario FCME (UNAM), a partir de la cual se determinaron 14 especies. Se amplía el conocimiento de este género para los estados de Chihuahua, Distrito Federal, Estado de México, Guanajuato, Guerrero, Michoacán, Nayarit, Oaxaca, Querétaro y Tlaxcala. Se proporciona una clave sinóptica para las especies de México y la descripción morfológica de algunas poco estudiadas.