ABSTRACT: Bovine spongiform encephalopathy (BSE) is a transmissible progressive neurodegenerative disease characterized by the accumulation of a pathological isoform (PrpSC) of the cellular prion protein (PrpC) in the brain of cattle. Two insertion/deletion polymorphisms in the PRNP gene (23bp in the promoter and 12bp in intron 1) have been associated with resistance or susceptibility to the disease. The aim of this study was to analyze the distribution of these polymorphisms in 214 healthy bovines belonging to four different breed groups (Aberdeen Angus, Aberdeen Angus x Hereford, Holstein Friesian and Uruguayan Creole cattle). DNA samples were amplified by end-point PCR. A high frequency of the alleles and haplotype associated with susceptibility to BSE (del12 and del23, and del12-del23, respectively) were found in the Aberdeen Angus, Aberdeen Angus x Hereford and Holstein Friesian animals. At the same time, the Uruguayan Creole cattle presented a higher frequency of the alleles and haplotype associated with resistance to BSE (ins12 and ins23, and ins12-ins23, respectively). These data could indicate a greater genetic resistance of the Uruguayan Creole cattle to BSE compared to other analyzed breeds, reinforcing its value as a zoogenetic resource. ABSTRACT (BSE PrpSC (PrpSC PrpC (PrpC insertiondeletion insertion deletion 23bp bp 1 21 . cattle) endpoint end point PCR del12 del (del1 del23 del12del23, del12del23 deldel del12-del23 respectively animals time ins12 ins (ins1 ins23 ins12ins23, ins12ins23 insins ins12-ins23 respectively. breeds resource 2 del1 (del del2 del12del2 del12-del2 ins1 (ins ins2 ins12ins2 ins12-ins2 del12del del12-del ins12ins ins12-ins

RESUMO: A encefalopatia espongiforme bovina (EEB) é uma doença neurodegenerativa progressiva transmissível dos bovinos, caracterizada pelo acúmulo no cérebro de uma isoforma patológica (PrpSC) da proteína priônica celular (PrpC). Dois polimorfismos de inserção/deleção no gene PRNP (23bp no promotor e 12bp no íntron 1) foram associados à resistência ou suscetibilidade à doença. O objetivo deste trabalho foi analisar a distribuição desses polimorfismos em 214 bovinos sadios, pertencentes a quatro diferentes grupos raciais (Aberdeen Angus, Aberdeen Angus x Hereford, Holstein Friesian e Crioulo Uruguaio). As amostras de DNA foram amplificadas por PCR de tempo final. Uma alta frequência dos alelos e haplótipos associados à suscetibilidade à BSE (del12 e del23 e del12-del23, respectivamente) foram encontrados nos animais Aberdeen Angus, Aberdeen Angus x Hereford e Holstein Friesian, enquanto o gado Crioulo Uruguaio apresentou maior frequência dos alelos e haplótipos associados à resistência à BSE (ins12 e ins23 e ins12-ins23, respectivamente). Esses dados podem indicar uma maior resistência genética do gado Crioulo Uruguaio à BSE em comparação com as outras raças analisadas, reforçando seu valor como recurso zoogenético. RESUMO EEB (EEB PrpSC (PrpSC PrpC. PrpC . (PrpC) inserçãodeleção inserção deleção 23bp bp 1 21 sadios Uruguaio. Uruguaio) final del12 del (del1 del2 del12del23, del12del23 deldel del23, del12-del23 respectivamente ins12 ins (ins1 ins2 ins12ins23, ins12ins23 insins ins23, ins12-ins23 respectivamente. analisadas zoogenético (PrpC 2 del1 (del del12del2 del12-del2 ins1 (ins ins12ins2 ins12-ins2 del12del del12-del ins12ins ins12-ins

SUMMARY: This study investigated the anatomy of the arteries of the brain, including the arterial circle of the brain, its branches and junctions, in five camel (Camelus dromedarius, Linnaeus 1758) following intravascular injection of colored latex via common carotid artery. The course and distribution of the arterial supply to the brain was described and morphological analysis was made. The basilar artery contributed to the blood supply of the brain in the camel in contrast to the situation in other Artiodactyla order.

RESUMEN: En presente estudio se analizó la anatomía de las arterias del encéfalo en cinco camellos (Camelus dromedarius, Linnaeus 1758). Después de administrar una inyección intravascular de látex coloreado en la arteria carótida común se estudiaron las arterias incluyendo al círculo arterial del cerebro, sus ramas y uniones. Fueron descritos en detalle el curso y la distribución del suministro arterial al encéfalo y se realizó un análisis morfológico. La arteria basilar contribuyó al suministro de sangre del encéfalo del camello, diferenciando este aspecto en otras especies de Artiodactyla.

SUMMARY: The available information on the cardiovascular apparatus of the capybara (Hydrochoerus hydrochaeris) is scarce. The general objective of this study was to describe the cardiac anatomy of this species. Six adult animals and five newborns were used; all were free of cardiovascular system pathologies. The method of study of the animals was simple dissection. The heart of the capybara was located from the second intercostal space to the sixth rib. The frenicopericardial ligament attached the fibrous pericardium to the sternum and the diaphragm. The intervenous tubercle was poorly developed. In the right atrium, there were few pectinated muscles. The right papillary muscles had septomarginal trabeculae among them. The carnous trabeculae showed a marked development on the marginal wall of the right ventricle. There were also large, carnous trabeculae on the septal wall, which was somewhat flatter. The left papillary muscles were two, located opposite each other and on the septal wall. Both were simple and sent tendinous cords to both cusps of the left atrioventricular valve. Carnous trabeculae of the left ventricle were less numerous. Between both papillary muscles there was a large carnous trabecula that occupied much of the dorsoventral extension of the septal wall of the left ventricle. Of both coronary arteries, the left coronary artery that emerged from the left coronary sinus of the aorta was the most important, with larger size and greater distribution. The paraconal interventricular branch was directed through the paraconal interventricular groove and terminated on the right ventricular margin anastomosing with the subsinusal interventricular branch that came through the groove of the same name. With other techniques, it is necessary to perform histological, semiological and physiological studies in the heart of this species to better understand its anatomy and physiology.

RESUMEN: La información disponible sobre el aparato cardiovascular del carpincho (Hydrochoerus hydrochaeris) es escasa. El objetivo general de este estudio fue describir la anatomía cardíaca de esta especie. Se utilizaron 6 animales adultos y 5 neonatos, libres de patologías del aparato cardiovascular. El método de estudio de los animales fue la disección simple. El corazón del carpincho estaba ubicado desde el segundo espacio intercostal hasta la sexta costilla. El ligamento frenicopericárdico unía el pericardio fibroso al esternón y al diafragma. El tubérculo intervenoso era de escaso desarrollo. En la aurícula derecha se encontraban escasos músculos pectinados. Los músculos papilares derechos presentaban entre ellos trabéculas septomarginales. Las trabéculas carnosas presentaban un marcado desarrollo sobre la pared marginal del ventrículo derecho. También había grandes trabéculas carnosas sobre la pared septal, que sin embargo era algo más lisa. Los músculos papilares izquierdos eran dos, situados opuestos uno al otro y sobre la pared septal. Ambos eran simples y emitían cuerdas tendinosas hacia ambas cúspides de la válvula atrioventricular izquierda. Las trabéculas carnosas del ventrículo izquierdo eran menos numerosas. Entre ambos músculos papilares existía una gran trabécula carnosa que ocupaba gran parte de la extensión dorsoventral de la pared septal del ventrículo izquierdo. De ambas arterias coronarias, la arteria coronaria izquierda que surgía del seno coronario izquierdo de la aorta era la más importante, de mayor tamaño y de mayor distribución, encargándose de la irrigación de la mayoría del territorio cardíaco. La rama interventricular paraconal se dirigía por el surco interventricular paraconal y terminaba sobre el margen ventricular derecho anastomosándose con la rama interventricular subsinusal que venía por el surco del mismo nombre. Con otras técnicas, es necesario realizar estudios histológicos, semiológicos y fisiológicos en el corazón de esta especie para entender mejor su anatomía y fisiología.

The laboratory rat (Rattus norvegicus albinus) has been used as a model for medical, biological and molecular research, for a long time. It is an interesting fact that there are no detailed descriptions of the gross anatomy of the liver and its ways of fixation. The aim of this study is to define clearly and according to the Nomina Anatomica Veterinaria principles, the liver and its way of fixation in this species of laboratory mammal. Eighty-eight rats (Rattus norvegicus albinus) were used, with a weight between 250 and 450 g. They were dissected in fresh, after been euthanized by an overdose of thiopental sodium intraperitoneal. An incision from the xiphoid cartilage up to the pubic region was made and another on each costal arch toward dorsal.

La rata de laboratorio (Rattus norvegicus albinus) ha sido usada como modelo para investigaciones médicas, biológicas y moleculares, desde hace mucho tiempo. Sin embargo, no existen descripciones detalladas del peritoneo digestivo de la rata wistar. El objetivo de este trabajo es definir en forma clara y acorde a los principios de la Nómina Anatómica Veterinaria el peritoneo en esta especie de mamífero de laboratorio. Se utilizaron ochenta y ocho ratas (Rattus norvegicus albinus) de entre 250 y 450 g. Fueron disecadas en fresco luego de ser eutanasiadas por sobredosis de tiopental sódico intraperitoneal. Se realizó una incisión paramediana que se extendía desde el cartílago xifoides del esternón hasta el pubis y otra sobre cada arco costal hacia dorsal.

The giraffe (Giraffa camelopardalis) is both the largest extant ruminant and a strict browser. We dissect and describe the macroscopic anatomy of the mouth of the giraffe. The heads of two adult giraffes and one fetus were used in this study. The lips were well developed, the upper one was predominant and dorsally flattened near the nostrils. The tongue had a lift or lingual torus and rostrally to it a groove-shaped depression or fossa linguae. There was no adipose body of cheek (Corpus adiposum buccae). The hard palate in the giraffe had 18 Rugae palatinae. The final roughness reaches the caudal border of the premolar 3. Caudal ridges had no papillae. The parotid gland was small and consisted of two lobes, one rostral and one caudal to be separated dorsally to accommodate the parotid lymph node. The parotid duct followed the same way as in the cow, ended in front of the upper premolar tooth 2 in the parotid papilla, (not evident at mucosal surface). Mandibular gland was divided into two lobes, the rostral one placed in the intermandibular space and the caudal hidden by the parotid gland. Giraffes have the monostomatic and polistomatic sublingual glands. The monostomatic sublingual gland was located rostrally and joined to the monostomatic of the other side in the very narrow rostral intermandibular space. The polistomatic sublingual gland was caudally located and reached the level of the third molar and at a deeper level than the monostomatic. The studied giraffes had dorsal, ventral and intermediate bucal salivary glands. Leaving aside the differences caused by different dimensions, the mouth of the giraffe had in general a similar anatomical arrangement to the cow.

La jirafa (Giraffa camelopardalis) es a la vez el rumiante más grande que existe y un ramoneador estricto. Nosotros disecamos y describimos la anatomía macroscópica de la boca de la jirafa. En este estudio se utilizaron las cabezas de dos jirafas adultas y de un feto. Los labios estaban bien desarrollados, el superior era el predominante y estaba aplastado dorsalmente cerca de las narinas. La lengua tenía una protuberancia o Torus lingual y rostralmente a él una depresión en forma de surco o Fossa linguae. No había cuerpo adiposo de la mejilla (Corpus adiposum buccae). El paladar duro en la jirafa tenía 18 rugae palatinae. Las rugosidades finales alcanzaban el borde caudal del premolar 3. Las crestas caudales no tenían papilas. La glándula parótida era pequeña y consistía de dos lóbulos, uno rostral y otro caudal que se separaban dorsalmente para acomodar al nódulo linfático parotídeo. El conducto parotídeo seguía el mismo trayecto que en la vaca, terminando frente al segundo diente premolar superior en la papila parotídea (no evidente en la superficie de la mucosa). La glándula mandibular estaba dividida en dos lóbulos, el rostral se colocaba en el espacio intermandibular y el caudal estaba oculto por la glándula parótida. Las jirafas tenían glándulas sublinguales monostomática y polistomática. La glándula sublingual monostomática estaba localizada rostralmente y se unía a la monostomática del otro lado en el muy estrecho espacio intermandibular. La glándula sublingual polistomática estaba localizada caudalmente y alcanzaba el nivel del tercer molar en un plano más profundo que la monostomática. Las jirafas estudiadas tenían glándulas salivares bucales dorsales, ventrales e intermedias. Dejando de lado las diferencias causadas por las diferentes dimensiones, la boca de la jirafa tenía en general una disposición anatómica similar a la de la vaca.