Borate bioactive glasses are more soluble than silicate’s and convert rapidly and completely into hydroxyapatite (Ca5(PO4)3(OH)), being more suitable for wound healing applications than their silicate counterparts. In this work, the 45B5 composition (46.1 B2O3 – 26.9 CaO – 24.4 NaO – 2.6 P2O5, mol%)) were embedded into electrospun PHBV (Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)) nanofibers by encapsulation and/or electrospray deposition aiming to produce a wound dressing with optimized bioactivity and antibacterial properties for wound healing applications. The fibers were characterized regarding their physical, structural, and thermal properties, and in vitro by L929 Mouse Fibroblast Cell Line adhesion, migration, and cytotoxicity and by its antibacterial activity against the bacteria S. aureus. The set of characterizations evidences that the encapsulation method was the most promising for the 45B5 embedding into PHBV nanofibers, as it produced a wound dressing with great loading efficiency (70%) with a highly hydrophilic surface, leading to expressive adhesion, migration, and viability of L929 cells and antibacterial activity. Thus, the nanofibers produced by the encapsulation method alone provided a dressing with high potential in wound healing management. silicates s Ca5PO43OH, Ca5PO43OH CaPOOH Ca5 PO4 3 OH , Ca PO (Ca5(PO4)3(OH)) counterparts work B 45B 46.1 461 46 1 (46. BO O B2O 269 26 9 26. 244 24 4 24. 2 6 2. P2O5 P mol% mol mol%) Poly3hydroxybutyrateco3hydroxyvalerate Polyhydroxybutyratecohydroxyvalerate Poly hydroxybutyrate co hydroxyvalerate (Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) andor or physical structural L L92 adhesion migration S aureus 70% 70 (70% surface Thus management (Ca5(PO4)3(OH) 46. (46 P2O hydroxybutyrateco (Poly(3-hydroxybutyrate-co-3-hydroxyvalerate L9 7 (70 (Ca5(PO4)3(OH (4 (7 (

Abstract Aiming to produce an antimicrobial dressing for wound healing applications, in this work Tea Tree oil (TTO) was incorporated into PHBV nanofibers by absorption. It was observed increase in the nanofiber diameter due to 5% TTO absorption efficiency, which also led to a 54% decrease in the contact angle. The releasing assay indicates a 6.8% oil release in the first 24 h – being probably the oil deposited at the polymer surface – followed by a minimal release at 48 h. The set of antimicrobial assays performed suggests the incorporation of TTO optimized the antimicrobial activity of the polymer for E. coli and C. albicans, while against S. aureus no significant difference was observed. The MTT assay showed no cytotoxicity of PHBV, but the incubation of L929 fibroblast cells with PHBV-TTO reduced cell viability. Overall, the PHBV nanofibers containing TTO present great potential as an antimicrobial dressing. applications (TTO 5 efficiency 54 angle 68 6 8 6.8 2 4 E C albicans S L L92 PHBVTTO viability Overall 6. L9