Abstract Based on debilitating recent budget cuts for science, Brazilian researchers had to find alternative ways to continue scientific production. Here we provide a perspective for the use of citizen-science data deposited in the iNaturalist platform as an alternative source of data to support biodiversity research. Observations contributed by volunteers can be analyzed at large spatial and temporal scales and can respond to questions in behavioral and population ecology. We analyzed this potential through the example of Brazilian amphibians, a group that is less studied worldwide than birds. In fact, to our knowledge, only two studies have been published that are based on citizen-science data for Brazilian amphibians. At the time of writing, the iNaturalist platform has over 14,800 research grade observations from Brazil, representing 698 species, a number increasing daily. Compared to other species-rich countries, volunteer-collected datasets from Brazil cover a relatively high taxonomic diversity (61%), providing a plethora of valuable data. Despite this potential, there are large spatial gaps in sampling in Brazil. Here we encourage established and budding herpetologists not only to use the platform to retrieve data, but also to contribute to iNaturalist actively, with new observations, as well as by identifying species in existing records.

PURPOSE: In the pathophysiology of sepsis tissue perfusion dysfunction is a crucial driving force. Thus the early recognition is highly important. Concerning the early hours of bacteremia, and the systemic inflammatory response reaction leading to sepsis we aimed to investigate the micro- and macrocirculatory changes. METHODS: In 20 juvenile Hungahib pigs were anesthetized and the femoral artery and external jugular vein were prepared unilaterally and cannulated. For assisted ventilation tracheostomy was performed. In Sepsis group (n=11) live E. coli was intravenously administered (increasing concentration, 9.5x10∧6 in 3h). In Control group (n=9) bacteria-free saline was administered at the same volume. Modified shock index (MSI), core and skin temperature, and skin microcirculation (laser Doppler) were measured before inducing bacteremia then hourly for 4h. RESULTS: In Control group parameters were stable, while six animals in the Sepsis group died before the 4th hour. Core and skin temperature did not show significant alterations. In Sepsis group microcirculation showed a large impairment already by the 1st hour, while in MSI only by the 3rd hour. CONCLUSION: During bacteremia and the early phase of sepsis microcirculatory impairment can be detected soon, even hours before the deterioration in hemodynamic parameters in this porcine model.