AbstractThe aim of this study was to analyze the impact that heat treatment with salts and freezing processes on the sensory, instrumental, and physico-chemical characteristics of fried potatoes of the Monalisa cultivar. The potatoes were blanched in distilled water (P); sodium chloride solution (B1); calcium chloride solution (B2), and a solution with both of these salts (B3). They were then pre-cooked and frozen for 24 hours and for 30 days. After frying, sensory characteristics were analyzed (color, texture, flavor, oiliness), along with overall preference and instrumental determinations of texture, color, and oil content. Further tests were conducted on the sample with the best results in the sensory analysis (B1), along with sample P as a control, to determine granule microstructure, carbohydrate fractions, glycemic index, and glycemic load. Blanching B3, despite reducing oil absorption and providing less oiliness, obtained lesser overall preference. Freezing for 30 days increased the lightness, except for when sodium chloride was used, which intensified the color yellow. The use of sodium chloride did not interfere with the type of starch granules, nor with the formation of resistant starch; however, longer freezing time reduced the glycemic index and concentrated the dietary fiber content. All samples exhibited low glycemic index and moderate glycemic loads.

Microbiological processes were used for chitin and chitosan productions by Cunninghamella elegans (UCP 542) grown in a new economic culture medium. The assay was carried out to evaluate the growth of C. elegans using yam bean (Pachyrhizus erosus L. Urban) medium, in different times of growth (24, 48, 72 and 96 hrs), incubated at 28ºC in an orbital shaker at 150 rpm. The lyophilized biomass was determined by gravimetry. The polysaccharides were extracted by alkali-acid treatment, and characterized by infrared spectroscopy, titration and viscosity. C. elegans grown in the yam bean medium and produced higher yields of biomass (24.3 g/ mL) in 96 hrs. The high level was chitosan (66 mg/g), and chitin (440 mg/g) were produced at 48 and 72 hrs of growth, respectively. The polysaccharides showed degree of deacetilation and viscosimetric molecular weight as: 6.2% and 3.25 x10(4) g/mol for chitin, and 85% and 2.72 x 10(4) g/mol for chitosan, respectively. The results obtained suggest high biotechnological potential of yam bean as an economic source to produce chitin and chitosan by C. elegans. In addition, the new medium using yam bean for production of the chitin and chitosan may be used for many purposes to reduce the cost price of fermentation processes.

A criptosporidiose é uma doença de importância para a saúde pública. A via de transmissão do Cryptosporidium parvum é associada freqüentemente à contaminação de água. Porém, estudos epidemiológicos revelam que sua incidência no ambiente aquático é subestimada. A falta de método apropriado de detecção para a pesquisa dos oocistos em amostras de água contribui para isso. O artigo discute vários aspectos do tema, dentre eles, a ocorrência desse patógeno no ambiente aquático e os avanços nos métodos de detecção. Apesar da existência de técnicas de biologia molecular para a identificação do agente, métodos parasitológicos ainda apresentam resultados satisfatórios. A reação em cadeia de polimerase (PCR) é um método de detecção promissor. A identificação dos oocistos de Cryptosporidium spp. na amostra de água é influenciada pelo número de oocistos presentes, pela sensibilidade do método e pelo método de concentração dos oocistos na amostra, cujo aperfeiçoamento é fundamental para a precisão da análise e pesquisa do parasito.

Criptosporidiosis is an important problem of public health. The role of Cryptosporidium parvum as a waterborne pathogen has been documented. But epidemiological features of this parasitic protozoon lead to the assumption that the incidence of Cryptosporidium spp. in aquatic environmental is under-estimated. The lack of sensitive detection methods adapted for oocysts detection in water contributed to this under-reporting. This review paper discusses the theme, including the occurrence of these pathogens in aquatic environmental and the available detection methods. Although the molecular biology technique used for identification of the agent, the parasitological diagnostics' method continue to show satisfactory results. Polimerase chain reaction (PCR) is a promising detection method and the potencial use in the detection of C. parvum in water has been described. The Cryptosporidium spp. oocysts identification in water samples depends on the number of oocysts, sensibilty of methods, and the concentration technique and this step is fundamentally to precision of test.

A criptosporidiose é uma doença de importância para a saúde pública. A via de transmissão do Cryptosporidium parvum é associada freqüentemente à contaminação de água. Porém, estudos epidemiológicos revelam que sua incidência no ambiente aquático é subestimada. A falta de método apropriado de detecção para a pesquisa dos oocistos em amostras de água contribui para isso. O artigo discute vários aspectos do tema, dentre eles, a ocorrência desse patógeno no ambiente aquático e os avanços nos métodos de detecção. Apesar da existência de técnicas de biologia molecular para a identificação do agente, métodos parasitológicos ainda apresentam resultados satisfatórios. A reação em cadeia de polimerase (PCR) é um método de detecção promissor. A identificação dos oocistos de Cryptosporidium spp. na amostra de água é influenciada pelo número de oocistos presentes, pela sensibilidade do método e pelo método de concentração dos oocistos na amostra, cujo aperfeiçoamento é fundamental para a precisão da análise e pesquisa do parasito.

Criptosporidiosis is an important problem of public health. The role of Cryptosporidium parvum as a waterborne pathogen has been documented. But epidemiological features of this parasitic protozoon lead to the assumption that the incidence of Cryptosporidium spp. in aquatic environmental is under-estimated. The lack of sensitive detection methods adapted for oocysts detection in water contributed to this under-reporting. This review paper discusses the theme, including the occurrence of these pathogens in aquatic environmental and the available detection methods. Although the molecular biology technique used for identification of the agent, the parasitological diagnostics' method continue to show satisfactory results. Polimerase chain reaction (PCR) is a promising detection method and the potencial use in the detection of C. parvum in water has been described. The Cryptosporidium spp. oocysts identification in water samples depends on the number of oocysts, sensibilty of methods, and the concentration technique and this step is fundamentally to precision of test.