Abstract: Background: Amaranthus hybridus L. (Amaranthaceae) is an annual, dicotyledonous species that is considered one of the main weed infesting agricultural production systems. Some weeds species are considered host plants to insect pests, serving as “green bridges” for subsequent attacks on the main crop during the season, which can cause significant losses. Recording the occurrence of insects that can reach pest status is important for the deployment of mitigation measures against possible damage and economic losses to crops. The presence of Spodoptera eridania (Cramer) (Lepidoptera: Noctuidae) has not been previously registered in association with A. hybridus plants. Objective: Thus, the present study is the first report to describe the occurrence of S. eridania on A. hybridus in the municipality of Piracicaba, São Paulo, Brazil. Methods: Images of the larvae while feeding on A. hybrids were obtained using an Olympus® E-410 digital camera. The pictures were then sent to the Laboratory of Ecotoxicology and IPM (LEMIP) of the Department of Entomology of Lavras Federal University (UFLA) for species identification. Results: We observed S. eridania larvae larger than 7 cm feeding on the inflorescences and leaves of A. hybridus . Conclusions: This note reports the first occurrence of S. eridania larvae feeding on A. hybridus in the city of Piracicaba, SP, Brazil.

Plant flavonoids have been increasingly studied for its importance in plant metabolism, inter-species interactions and human health-promoting effects. Each botanical species has a distinct flavonoid profile, and as such, this work aimed to develop and apply a high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) method to quantify specific flavonoids in different plants and parts. Six flavonoids (isoquercitrin, quercetin, naringin, naringenin, hesperidin and rutin), two isoflavonoids (daidzin and daidzein) and one coumarin (umbelliferone) were targeted. Calibration curves showed good correlations (R2 > 0.994) and limits of quantification (≤ 500 ng mL-1). The method was applied for detection of analytes in common bean seeds (Phaseolus vulgaris), soybean leaves (Glycine max), and sour orange (Citrus aurantium), sweet orange (Citrus sinensis), and Tahiti lime (Citrus latifolia) flavedo after ultrasound assisted acidic hydroalcoholic extraction. The method was tuned for quick and efficient extraction. Principal component analysis was helpful for the discrimination of matrices based on their flavonoid profiles.

Avaliou-se, em condições de laboratório, a resistência de genótipos de tomateiro, Lycopersicum esculentum(Miller) (cv. ‘Santa Clara' e híbridos: Saladete Italiano Ty Tyna, Santa Cruz Débora Ty, Salada Lumi e Saladete Italiano Andrea Victory), Lycopersicum hirsutum f. glabratum (C.H. Mull.) (linhagens PI 134417 e PI 134418) e Lycopersicum pimpinellifolium (Just) (linhagens NAV 1062 e PI 126931) sobre Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae). A linhagem PI 134418 foi, dentre os genótipos avaliados, a que apresentou efeito mais negativo sobre T. absoluta, em relação à testemunha (Santa Clara), alongando a fase larval, reduzindo a sobrevivência larval e a sobrevivência global. A linhagem PI 134417 também prejudicou o desenvolvimento de T. absoluta, alongando a fase larval e reduzindo a sobrevivência larval. Os demais genótipos não apresentaram efeito negativo sobre a biologia de T. absoluta. Concluiu-se que PI 126931 é suscetível, e NAV 1062, Santa Clara, Tyna, Débora, Lumi e Andrea Victory são altamente suscetíveis a T. absoluta, enquanto PI 134418 e PI 134417 destacaram-se com alta resistência e moderada resistência, respectivamente, a T. absoluta, do tipo antibiose.

The resistance of tomato genotypes, Lycopersicon esculentum (Miller) (cv. Santa Clara, hybrids: Saladette Italian Ty Tyna, Santa Cruz Débora Ty, Salad Lumi and Saladette Italian Andrea Victory), Lycopersicon hirsutum f. glabratum (C,H, Mull.) (lines PI 134417 and PI 134418) and Lycopersicum pimpinellifolium (Just) (lines NAV 1062 and PI 126931) to Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) was evaluated under laboratory conditions. The line PI 134418 had the most negative effect on T. absoluta in comparison to the control (Santa Clara), lengthening the larval stage and reducing larval and global survival. The line PI 134417 also affected the development of T. absoluta lengthening the larval stage and reducing the larval survival. The other genotypes showed no negative effect on the biology of T. absoluta. We concluded that PI 126931 is susceptible and NAV 1062, Santa Clara, Tyna, Débora, Lumi and Andrea Victory are highly susceptible to T. absoluta, whereas PI 134418 and PI 134417 highlighted with high resistance and moderate resistance, respectively, to T. absoluta of antibiosis-type.