ABSTRACT. Bahiagrass (Paspalum notatum Flügge) is an important forage in South America and the United States because of its high palatability, resistance to trampling and grazing, and tolerance to cold, but it exhibits low seed yield and poor seed quality. Previous studies reported improvements in forage production and nutritive value in hybrids and ecotypes; however, information about seed yield and quality in forage bahiagrass is limited. This study aimed to characterize the seed yield and quality of nine P. notatum intraspecific hybrids and three controls: P. notatum ecotypes V4 and Bagual and cultivar Pensacola. Inflorescence density, 1,000-seed weight, seed yield and germination rate decreased in year 2 influenced by weather conditions and ergot. Seed yield ranged from 139 (Pensacola) to 1,158 (Bagual) kg ha-1 among all entries, where Bagual, C18, and V4 produced more than 974 kg ha-1, which was approximately seven times more than Pensacola. Bagual, C18, V4, 336, C15, 225, and D3 showed germination rates greater than 83%. In conclusion, Bagual had the highest seed yield and germination rate, whereas hybrids C18 and 336 showed high seed yield, 1,000-seed weight, and germination rate in both years. Bagual, C18, and 336 should be used in future breeding programs to improve seed production traits. Our study revealed that selection for seed production traits can result in improvements in seed yield and quality in bahiagrass. ABSTRACT Paspalum Flügge palatability grazing cold however limited P controls V Pensacola density 1,000seed 1000seed 1,000 1 000 weight ergot 13 (Pensacola 1158 158 1,15 (Bagual ha1 ha ha- entries C 97 ha1, 1, C15 225 D 83 83% conclusion C1 33 years 000seed 1000 1,00 00 115 15 1,1 9 22 8 3 100 1,0 0 11 10

ABSTRACT The objective of this study was to estimate the genetic parameters and predict selection of genetic gains and genetic diversity of 12 Andropogon lateralis ecotypes collected in the State of Rio Grande do Sul, Brazil. To estimate genetic parameters and predict selection gains, the REML/BLUP technique was applied. Genetic diversity among the ecotypes was evaluated by two clustering methods (optimization and hierarchical) and principal component (PC) analysis, the latter method also used to discard variables. The genetic parameters studied showed high potential for selection of important agronomic forage traits for livestock production. Results showed that the 12 A. lateralis Nees ecotypes exhibited high genetic variability for the studied forage characters and indicated parental prosperity for crosses within the genetic breeding program. Principal component analysis showed that number of total vegetative tillers, leaf:stem ratio, number of reproductive tillers, and leaf dry matter yield accounted for 80.6% of the observed variation in PC1. These variables are important characteristics for quantifying the dry matter production and nutritional value of forage plants, and they can help to discriminate amongst ecotypes. Ecotypes sourced from the Pelotas, Piratini, Passo Fundo, Bagé, and Montenegro regions showed superior forage production when evaluated by the BLUP methodology. Therefore, this group was identified as the most suitable for selection and crossing purposes. Tocher’s cluster analysis grouped the ecotypes into five divergent groups. Principal component and UPGMA hierarchical methods were also efficient at separating the ecotypes. 1 Sul Brazil REMLBLUP REML applied optimization PC (PC A program tillers leafstem stem ratio 806 80 6 80.6 PC1 plants Pelotas Piratini Fundo Bagé methodology Therefore purposes Tochers Tocher s groups 8 80.

ABSTRACT The objective of this study was to verify the repeatability of the expression of forage characters in intraspecific hybrids of Paspalum notatum Flügge to aid early selection. Across five harvests, plant height, tiller population density, leaf dry matter, stem dry matter, inflorescence dry matter, total dry matter, and growth habit were quantified for five parents, 189 hybrids, and a commercially available cultivar as a control (n = 195). Analysis of variance, principal components analysis, and structural analysis methods were used to determine the repeatability coefficients. The repeatability coefficients ( ρ ^) for all evaluated characteristics generated by the different methods were between 0.05 (ANOVA II) and 0.95 (PCACov). For most of the characteristics studied, repeatability coefficients and determination coefficients were considered high. The repeatability coefficients estimates obtained for the eight characteristics evaluated with the ANOVA I and II methods were almost always lower than those obtained by PCA and structural analysis methods. Based on the covariance matrix, the principal component method generated higher estimates than those produced by ANOVA or structural analysis. Assuming a minimum 80% reliability to verify the relative superiority of the hybrids across all assessed traits, the five harvests proved adequate for selecting the optimal plant materials to advance to the next phase of the breeding program. However, reliable early selection for leaf dry matter, leaf:stem ratio, and total dry matter required a minimum of two harvests. The genetic parameters (h2 and CVg) showed a favorable scenario for direct selection to increase forage production. height density parents 18 n 195. 195 . 195) variance ^ 005 0 05 0.0 095 95 0.9 PCACov. PCACov (PCACov) studied high matrix 80 traits program However leafstem ratio h2 h (h CVg production 1 19 00 0. 09 9 (PCACov 8

Abstract The Paspalum genus has potential for further genetic improvement because of its adaptability to different ecosystems and production of high yields for grazing livestock. We estimate the genetic parameters of 195 intraspecific P. notatum hybrids using Restricted Maximum Likelihood (REML), followed by selection based on Best Linear Unbiased Prediction (BLUP) through multivariate analysis. The intraspecific hybrids studied showed considerable genetic variability in the evaluated forage traits, displaying their potential for progression in subsequent stages of the genetic improvement program. Notably, plant height emerged as an important trait for indirect selection to enhance forage production. The use of the REML/BLUP procedure proves to be a robust tool for data analysis, particularly for perennial species. Furthermore, multivariate analysis based on BLUPs should be used in the selection process within breeding programs. Based on the BLUP values, hybrids D3, D16, C17, C2 and B17 were identified as superior for forage production, and they hold promise for future breeding programs for future breeding initiatives aimed at direct selection to improve yield. livestock 19 P REML, REML , (REML) (BLUP traits program Notably REMLBLUP species Furthermore values D3 D D16 C17 C B B1 yield 1 (REML D1 C1

RESUMO: Andropogon lateralis Nees é uma gramínea nativa do sul do Brasil e uma das espécies mais frequentes na flora dos campos nativos da região. Possui ampla distribuição e alto grau de plasticidade fenotípica, tornando-o adaptável as condições edafoclimáticas e a práticas de desfolha. Este estudo teve como objetivo avaliar a dissimilaridade entre doze ecótipos de A. lateralis N., coletados em diferentes regiões do Rio Grande do Sul, submetidos a duas frequências de desfolha e três alturas de corte. No período de setembro a fevereiro, os ecótipos foram avaliados quanto à produção de matéria seca total, de lâminas foliares e de colmo. Além disso, foram medidos o número de perfilhos totais, vegetativos e reprodutivos e a altura das plantas. Essas características são importantes para a seleção de genótipos superiores em termos de variabilidade genética e produção de forragem. A matéria seca total e a matéria seca foliar são características com importância agronômica e apresentaram a maior correlação (r = 0,77), possibilitando uma seleção indireta para uma dessas características. A seleção natural das plantas resultou em características estruturais, morfológicas e produtivas distintas com uma heterogeneidade que permite a seleção e agrupamento de acordo com as características, ecótipos com características agronômicas superiores podem ser incluídos em programas de melhoramento.

ABSTRACT: Andropogon lateralis Nees is a native grass of southern Brazil and is one of the most frequent specie found in native grasslands. The species is widely distributed and has a high degree of phenotypic plasticity, which makes it highly adaptable to different edaphoclimatic conditions and management. This study aimed to evaluate the behavior of twelve ecotypes of A. lateralis, collected in different regions of the state of Rio Grande do Sul and cut to three different heights and subjected to two different defoliation frequencies. From September to February, the ecotypes were evaluated for total dry matter, leaf and stem yields. In addition, total, vegetative and reproductive tillers and plant height were measured. These characteristics are important for the selection of superior genotypes in terms of genetic variability and forage production. Total dry matter and leaf dry matter are characteristics with agronomic importance and they showed the highest correlation (r = 0.77), enabling an indirect selection for one of these characteristics. The natural selection of plants resulted in distinct structural, morphological and productive characteristics with heterogeneity that allows the selection and grouping according to the characteristics, ecotypes with superior agronomic characteristics can be included in breeding programs.

ABSTRACT The objective of this study was to evaluate 94 Paspalum notatum genotypes over two growing seasons to estimate genetic dissimilarity through agronomic traits and the distance between genotypes. This information is used to create an ideotype from the best averages obtained for the set of characteristics evaluated. Seven apomitic, three sexual, and 81 hybrid genotypes were compared with native genotypes “André da Rocha”, “Bagual”, and cultivar “Pensacola” as controls. There is genetic variability in P. notatum for the studied characteristics, and distinct genotypes with superior characteristics can be used in new combinations between apomictic and sexual plants to obtain hybrids. The characters with the greatest relative contribution to the dissimilarity between the genotypes were tiller density, stem dry mass, and leaf dry mass yield. Thus, these characteristics are suitable criteria to infer genetic distance studies in P. notatum . The selection index based on the ideotype is an auxiliary tool in the breeding process. The ideotype must be based on characteristics of interest according to the objective of the breeding program, as well as on the breeder’s prior knowledge in relation to culture.

Abstract Paspalum notatum is an important forage native to Southern Brazil and one of the most promising fodder species in terms of productivity, quality, resistance and growth speed. The objective of this study was to evaluate the reproductive mode and fertility of hybrid progenies resulting from artificial crosses among artificially duplicated sexual plants, selected intraspecific hybrids of the UFRGS breeding program and apomictic ecotypes of the species. The resulting F1 progenies were represented by 24 plants with high pollen viability (varying from 82.15 to 99.67%) and 1.2:1 segregation for the sexual: apomictic reproduction mode, indicating predominantly sexual hybrids, due to distorted segregation. In the apomictic plants, most chromosomes paired as bivalents in diakinesis. Since the low presence of abnormalities observed in the meiotic chromosome pairing and the high pollen viability of the hybrids, all plants were confirmed as promising parents for future crosses.

Abstract Angelonia integerrima Sprengel is a native species of the state of Mato Grosso do Sul and the Southern region (Paraná, Santa Catarina and Rio Grande do Sul) of Brazil, with features such as an unusual appearance and color of the flowers, indicating an ornamental use. To optimize the use of this species and to fill in gaps regarding its cytogenetic characterization, this study determined the chromosome number, meiotic index and pollen viability of plant individuals of four A. integerrima populations. All plant individuals of the four populations had 2n = 20 chromosomes. Still, the meiotic index of most analyzed plant individuals exceeded 90%, while pollen viability of all plant individuals was higher than 80%. These data suggest considerable cytological stability of the analyzed A. integerrima plant individuals, which may favor the selection of future genotypes for commercial purposes or their use in conservation and breeding programs of the species.

Abstract Paspalum notatum is a polymorphic species with two accepted varieties, according to the ploidy level. The ploidy level is closely related to the reproductive mode of this species. Usually, diploid cytotypes have sexual reproduction, and tetraploid cytotypes have an apomictic reproduction. Apomixis limits genetic recombination, hindering the development of new cultivars. The identification of sexual plants represents many possibilities in breeding programs. This study aimed to analyze the chromosome number in P. notatum accessions under agronomic evaluation. Ploidy level was determined in 25 accessions. Two ploidy levels were detected, resulting in 21 tetraploid and four diploid accessions. The four diploid accessions originate from Argentina and Uruguay, in a region close to the origin of Pensacola, which is a diploid sexual cultivar of P. notatum. These new diploid accessions may be useful in the development of breeding programs of Paspalum species.

ABSTRACT: For many decades, animal production in southern Brazil has been based on native forage grasses. Paspalum notatum Flügge (bahiagrass) is one of the most frequently used native grass in southern Brazil. The native germplasm of P. notatum is tetraploid and displays apomictic reproduction; hence, chromosome doubling of sexual diploid accessions is required to facilitate hybridization. The main goal of this study was to evaluate forage production and other agronomical evaluation of P. notatum intraspecific hybrids in order to obtain new varieties that could be registered and/or protected and launched as new cultivars in the future. Three tetraploid sexual genotypes were crossed with the ecotypes Bagual and André da Rocha. Biomass production (leaf, stem, and inflorescence fractions), plant growth habit, plant height, number of tillers, and frost damage were evaluated in the resulting hybrids. The general combining ability (GCA) and specific combining ability (SCA) were calculated for the six families generated. We evaluated 196 hybrids and the most productive yielded 42 % more total dry mass than the most productive parent (ecotype Bagual), and Bagual production was seven-fold higher than that of cultivar ‘Pensacola’. The highest GCA values were observed for Bagual and Q4205. No correlation was observed between leaf coloration and the frost effect or regrowth in the following winter. Similarly, no correlation between plant growth habit and dry mass production was observed. The reproduction mode of the most productive hybrids are currently being analyzed in larger plots to identify apomictic and sexual genotypes for future registration and protection.

ABSTRACT The objective of this study was to assess the genetic diversity among accessions of Paspalum notatum based on morphological marker traits and identify those morphological traits that contribute most to the variability among accessions. Fifty-three accessions were evaluated for the following traits: length, width, color, and pubescence of sheath; length, width, insertion angle, color of midrib, and pubescence of leaves; number, length, and angle of racemes; length of flowering stems; and plant growth habit (erect or prostrate). Multivariate analysis of the quantitative morphological data based on the Mahalanobis distance and UPGMA method grouped the accessions into 19 heterogeneous clusters. The relative contribution of each trait to cluster formation was obtained using the Singh method. Qualitative morphological traits were used to construct a dendrogram based on Jaccard's similarity, generating 13 clusters. Based on the morphological traits investigated, there is high genetic variation among accessions of P. notatum, demonstrating that these accessions have a good potential for a breeding program. Length of racemes, width of sheath, length of flowering stems, and length of leaves were found to contribute most to the variability among accessions. A number of traits contributed to a lesser degree. The set of characters used are representative of the vast phenotypic plasticity observed in P. notatum, a diversity which is likely to be related to the species apomictic form of reproduction and the origin of the accessions.

ABSTRACT The Paspalum genus includes several species that are important for livestock in Rio Grande do Sul, such as P. notatum and P. guenoarum, typical of native pastures of the Pampa biome. The aim of this study was to investigate forage production and chemical composition of four ecotypes of these species in relation to the cv. ‘Pensacola’ (P. notatum). Ecotypes of P. guenoarum (Azulão and Baio) and P. notatum (André da Rocha and Bagual) and the cv. ‘Pensacola’ were evaluated for two years, during which four cuts/year were made. The work was carried out under field conditions at the Agronomic Experimental Station of the Federal University of Rio Grande do Sul (30°05’S; 51°39’W), in a completely randomized design. P. guenoarum stood out for higher productivity and greater tolerance to cold; the Azulão ecotype showed more autumn production in relation to the other ecotypes. Crude protein content ranged from 14 (Baio) to 15% (‘Pensacola’); for neutral detergent fiber, the variation was 68 (Azulão) to 71% (‘Pensacola’) and for acid detergent fiber there was a variation of 38 (‘Pensacola’) to 43% (Baio). The data demonstrates the potential of native genotypes for use as cattle feeding in southern Brazil.

ABSTRACT The aim of this study was to determine the mode of reproduction of a hybrid progeny derived from intraspecific crosses of Paspalum notatum through cytoembryological analysis and use of RAPD (random amplification of polymorphic DNA) molecular markers. Cytoembryological analysis allowed identification of the mode of reproduction of 28 plants that were selected after agronomic productivity evaluations. Of these, 19 had embryo sac morphology compatible with an apomictic mode of reproduction and nine had embryo sac morphology compatible with a sexual mode of reproduction. Meanwhile, molecular marker analysis for 194 individuals showed 54 sexual and 140 apomictic plants; of the 28 plants analyzed by the two methods, ten results (35.7%) were in disagreement. In this paper, through cytoembryological analyses, a ratio of 1:2.1 of sexual to apomictic plants was found. The BCU 243 marker showed a stable pattern of amplification, but some results differed with cytoembryological analyses, demonstrating that these analyses are more reliable when determining the mode of sexual reproduction for the plants of P. notatum. Apomictic plants characterized in this work can be tested in the field to check their agronomic value and registration as plant varieties, while the sexual plants can be used as potential parents in future crosses.

RESUMO O objetivo deste estudo foi avaliar a expressão de caracteres agronômicos e a tolerância ao frio de híbridos apomíticos de Paspalum plicatulum x P. guenoarum, bem como estimar suas correlações fenotípicas, em duas regiões edafoclimáticas distintas. O estudo foi conduzido em diferentes locais e anos de avaliação, em delineamento de blocos ao acaso, com três repetições. Foram avaliados quatro híbridos interespecíficos de Paspalum, dois genótipos de P. guenoarum e a cv. Aruana (Panicum maximum), utilizada como testemunha. Os híbridos H12, H13, H20 e H22 apresentaram superioridade para os caracteres agronômicos avaliados e tolerância ao frio, em relação às cvs. Rojas e Aruana e ao ecótipo Azulão, e são indicados para novas etapas de avaliação, como estimativas da produção de sementes e estudos de DHE e VCU, visando o lançamento como novas cultivares. As estimativas de correlações fenotípicas são importantes ferramentas para a seleção de progênies, com destaque para o caráter MSF que apresentou alta correlação positiva com os caracteres MST, DPP, rebrote e altura, indicando ser desnecessária a realização de separação morfológica, economizando tempo e mão de obra em um programa de melhoramento de forrageiras.

ABSTRACT The aim of this study was to evaluate the expression of agronomic characteristics and tolerance to cold in apomictic Paspalum plicatulum x P. guenoarum hybrids, and estimate their phenotypic correlations in two different regions of soil and climate. The study was conducted at different locations and evaluated in different years, in a randomised block design with three replications. Four interspecific hybrids of Paspalum and two genotypes of P. guenoarum were evaluated, with the Aruana cultivar (Panicum maximum) used as a control. The H12, H13, H20 and H22 hybrids proved to be superior in tolerance for cold and the agronomic traits evaluated relative to the Rojas and Aruana cultivars and the Azulão ecotype, and are suitable for new stages of assessment, such as estimates of seed production and studies of DHS and VCU, with a view to their release as new cultivars. Estimates of phenotypic correlations are important tools in progeny selection, especially the characteristic of LDM, which showed a high positive correlation with the characteristics TDM, PDP, regrowth and height, demonstrating that carrying out morphological separation is unnecessary, saving time and labour in a forage breeding program.

RESUMO O objetivo do trabalho foi fornecer subsídios para obtenção de ganho genético em espécies apomíticas do gênero Paspalum pela identificação de caracteres que evidenciem simultaneamente alta correlação e herdabilidade. Assim, dimensionar o quanto da variação fenotípica é devido a efeitos genéticos e ambientais e o grau de associação existente entre a produção da matéria seca de folha como outros caracteres de interesse forrageiro de mais fácil seleção e aferição, proporcionará agilidade e economia no processo de seleção de plantas forrageiras. O experimento foi conduzido nos anos de 2009/10 e 2010/11 nos municípios de Eldorado do Sul e Augusto Pestana, RS, Brasil, em um delineamento experimental de blocos casualizados com três repetições. Nos acessos apomíticos do gênero Paspalum o aumento da pressão de seleção direta sobre a matéria seca total e de folhas é fortalecida pela maior contribuição dos efeitos genéticos do que ambientais sobre a composição da variância fenotípica. O ganho genético na produção de folhas se mostra eficiente via seleção indireta pela matéria seca total, caráter de elevada herdabilidade e de mais fácil seleção e aferição.

ABSTRACT The objective of this work was to provide means to obtain genetic gain in apomictic species of the genus Paspalum by identifying traits that simultaneously show high correlation and heritability. Thus, to determine the amount of phenotypic variation due to genetic and environmental effects and the degree of association between leaf dry matter production and other traits of forage interest that are easy to select and measure will bring agility and economy in the selection of forage species. The experiment was conducted in the years 2009/10 and 2010/11 in the municipalities of Eldorado do Sul and Augusto Pestana, RS, Brazil, in a randomized complete block design with three replicates. The increase in the direct selection pressure on total and leaf dry matter of apomictic accesses of the genus Paspalum is enhanced by a greater contribution of genetic effects than environmental effects on the composition of the phenotypic variance. Efficient genetic gain in leaf production is achieved by indirect selection of total dry matter, which is a trait of high heritability and easy to select and measure.