Nos estudos de obtenção de materiais zeolíticos é comum a formação de misturas de fases cristalinas devido à estabilidade relativa destes materiais no meio reacional empregado na síntese. Desta forma, é extremamente importante quantificar as diversas fases presentes no sistema para otimizar os métodos de sínteses de uma determinada zeólita sem a presença de contaminantes. Especificamente, foi verificado que durante a síntese das zeólitas ZSM-5(MFI), mordenita (MOR) e ferrierita (FER), em diversas condições experimentais, ocorreram a formação de misturas de fases binárias (FER+MOR) e (FER+MFI), às vezes ternárias (FER+MOR+MFI). O propósito do trabalho foi a realização de várias misturas binárias em diversos percentuais destes materiais, a fim de se obter uma metodologia para quantificar a presença de cada uma das fases (FER, MOR e MFI) em amostras sintetizadas sob diferentes condições experimentais. Com este objetivo, foram preparadas misturas físicas com diversos teores das fases MFI, MOR e FER, a partir de amostras altamente cristalinas de cada fase. Estas fases de composições conhecidas foram analisadas por difração de raios X para quantificar as áreas dos picos característicos de cada material, podendo-se assim obter uma relação de percentual de fase/área de cada componente da mistura.

In studies for obtaining zeolitic materials common to the formation of mixtures of crystalline phases due to the relative stability of these materials in the medium used in the synthesis. Thus, it is extremely important to quantify the various phases present in the system to optimize methods of synthesis of a zeolite without determining the presence of contaminants. Specifically, it was found that during the synthesis of zeolites ZSM-5(MFI), mordenite (MOR) and ferrierite (FER) in various experimental conditions, is the formation of binary mixing phases (FER + MOR) and (FER + MFI), sometimes ternary (FER + MOR + MFI). The purpose of this work was to perform various binary mixtures at various percentages of these materials in order to obtain a methodology to quantify the presence of each of the stages (FER, MOR and MFI) samples synthesized under different experimental conditions. With this objective, physical mixtures were prepared with different contents of the phases MFI, MOR and FER, from highly crystalline samples of each phase. These known compositions phases were analyzed by X-ray diffraction to quantify the areas of the peaks characteristic of each material, thus being able to obtain a ratio of the percentage of phase/area of each blend component.

The main purpose of this study was to investigate the effect of thyroxine and PTU in ascitic Ehrlich tumor cells. Tumor was implanted in 30 female mice distributed in three groups: treated with PTU, treated with thyroxine and control. Each group received an intraperitoneal injection of neoplastic cells, pre-incubated with sterile solutions of PTU, thyroxine and distilled water, respectively. On the fifth and seventh days after inoculation, animals received an intraperitoneal injection of the respective solutions. On the tenth day after inoculation, animals were sacrificed. Volume of ascitic liquid, number of neoplastic cells/ml and percentage of viable cells were determined. Ascitic liquid smears were carried out for tumor cytological evaluation. There was no difference among groups regarding ascitic liquid and as for the number and viability of tumor cells. However, cells under the effect of thyroxine presented significantly larger mean of nuclear diameter, size and number of nucleolus organizer regions. In this group, there was a predominance of clear, round cells with abundant eosinophilic and very vacuolated cytoplasm with little defined edges. Under the PTU effect, tumor cells were small with hyperchromatic nucleus and the same number of NORs as the control group. It was concluded that PTU and thyroxine have not changed the number and viability of cells after 10 days of tumor inoculation but they changed significantly cell characteristics. Whilst thyroxine increases cell size and the number of NORs of ascitic Ehrlich tumor cells, PTU causes an opposite effect

El propósito principal de este estudio ha sido investigar el efecto de la tiroxina y del propiltiouracilo (PTU) en las células del tumor de Ehrlich. El tumor fue implantado en 30 ratones hembras distribuidas en tres grupos: tratado con PTU, tratado con tiroxina y control. Cada grupo recibió una inyección intraperitoneal de células neoplásicas, pre-incubadas con las soluciones estériles de PTU, tiroxina y agua, respectivamente. En el quinto y séptimo días después de la inoculación, los animales recibieron una inyección intraperitoneal de las soluciones respectivas. En el décimo día después de la inoculación, se sacrificaron los animales. Fueron determinados el volumen de líquido ascítico, el número de células/ml y el porcentaje de células viables. Además se realizaron frotis del líquido ascítico para la evaluación de la citología del tumor. No hubo ninguna diferencia entre los grupos con respecto al volumen del líquido ascítico y el número y viabilidad de las células del tumor. Sin embargo, las células bajo el efecto de la tiroxina presentaron una media significativamente superior del diámetro nuclear, tamaño y número de regiones organizadoras de nucleélos. En este grupo, había un predominio de células claras, redondas con citoplasma abundante, eosinofílico y vacuolado con poca definición de los bordes. Bajo el efecto del PTU, las células del tumor eran pequeñas con el núcleo hipercromático y el mismo número de NORs como el grupo control. Se concluye que el PTU y tiroxina no afectaron el número y viabilidad de las células después de 10 días de inoculación del tumor, pero sí cambiaron las características celulares. Aunque la tiroxina aumenta el tamaño celular y el número de NORs de las celulas del tumor de Ehrlich, PTU causa efectos opuestos