Abstract We announce the mitochondrial genomes of seven species of the genus Sporophila (S. bouvreuil, S. iberaensis, S. melanogaster, S. minuta, S. nigrorufa, S. pileata, and S. ruficollis) which were validated by comparative genomic and phylogenetic analysis with related species. The mitochondrial genomes of seven passerines of the genus Sporophila were assembled (three complete and four nearly complete genomes) and were validated by reconstructing phylogenetic relations within Thraupidae. The complete mitogenomes ranged from 16,781 bp in S. ruficollis to 16,791 bp in S. minuta. We identified a conserved genome composition within all mitogenomes with 13 protein-coding genes, 22 tRNAs and two rRNAs. We observed a bias in the nucleotide composition and six mutational hotspots in Sporophila mitogenomes. Our mitogenome-based phylogenetic tree has S. minuta, S. maximiliani and S. nigricollis as sister species of the remaining species in the genus. We present new mitogenome sequences for seven Sporophila species, providing new genomic resources that may be useful for research on the evolution, comparative genetics, and conservation of this threatened group. S (S bouvreuil iberaensis melanogaster minuta nigrorufa pileata three Thraupidae 16781 16 781 16,78 16791 791 16,79 1 proteincoding protein coding genes 2 rRNAs mitogenomebased based evolution genetics group 1678 78 16,7 1679 79 167 7 16,

Abstract Until recently, no native mushroom was considered food in the Cerrado biome, while there are records of the occurrence of about 638 species of fungi so far, many of which have numerous undefined potentials, with the potential for food among them. Our work presents the nutritional aspects of two mushroom species found in an agroforestry system in the Cerrado (Auricularia nigricans and Schizophyllum commune), and a morphological identification key for the edible mushroom species highlighted in this study. The nutritional composition analysis revealed a moisture content of 65 and 41%, proteins of 7 and 10%, ether extract (fat) of 1.3 and 1.5% and fiber of 2 and 2.2% for A. nigricans and S. common, respectively. In this work we highlight the nutritional potential of two species of edible mushrooms, A. nigricans and S. commune. These mushrooms show similar nutritional characteristics to those of commercially produced exotic mushrooms, being rich in proteins and minerals. These data, added to other reports of this nature recently presented, reinforce the importance of knowledge of local biodiversity and native mushrooms as a food source.

RESUMO Este estudo avaliou a qualidade da silagem de duas cultivares de cana-de-energia (Saccharumspontaneum) produzidas com diferentes aditivos. O experimento foi instalado em delineamento inteiramente casualizado, com arranjo fatorial 2 × 5 de tratamentos composto por duas cultivares (VG3, VG1126) e quatro aditivos (1% uréia; 1% NaOH; 1% CaO; e 1,0 × 1011 UFC g -1 de Lactobacillus plantarum- LP) + controle, com oito repetições. Foram utilizados minisilos experimentais, os quais foram abertos 72 dias após a ensilagem. Os teores de matéria seca (MS), proteína bruta (PB), matéria orgânica (MO), matéria mineral (MM), extrato etéreo (EE), fibra em detergente neutro (FDN) e fibra em detergente ácido (FDA) e degradabilidade da silagem foram avaliados. Identificou-se efeito de interação significativo entre cultivar e tipo de aditivo para MS, PB, EE, FDN. ADF. As porcentagens de matéria orgânica e mineral foram semelhantes entre as duas cultivares. LP foi o aditivo menos benéfico para a degradação da MS. Após 72 h de fermentação, a degradação in vitro da FDN da cultivar VG3 aumentou devido à uréia, NaOH e CaO, enquanto a degradação da cultivar VG1126 diminuiu quando inoculada com LP ou CaO. O NaOH e o CaO melhoraram a degradabilidade da silagem, melhorando assim sua qualidade. O uso da LP no presente estudo não beneficiou a silagem para cultivares de cana-de-energia. O uso de NaOH e CaO melhora a qualidade da silagem, duas variedades de energia da cana-de-açúcar VG3 e VG1126, possibilitando seu uso com melhor eficiência para ração animal

ABSTRACT This study examined the quality of silage from two cultivars of energy cane (Saccharum spontaneum) produced with different additives. The experiment was laid out in a completely randomized design with a 2×5 factorial arrangement of treatments consisting of two cultivars (VG3, VG1126) and four additives (1% urea; 1% NaOH; 1% CaO; and 1.0 × 1011 CFU g-1 of Lactobacillus plantarum- LP) + control, with eight replicates. Experimental mini-silos were used, which were opened 72 days after ensiling. The dry matter (DM), crude protein (CP), organic matter (OM), mineral matter (MM), ether extract (EE), neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents, and silage degradability were. We identified significant interactions effect between cultivar and type of additive for DM, CP, EE, NDF. ADF. Organic and mineral matter percentage were similar between the two cultivars. LP was the least beneficial additive for DM degradation.After 72 h of fermentation, in vitro NDF degradation of cultivar VG3 increased due to urea, NaOH, and CaO, whereas degradation cultivar VG1126 decreased when inoculated with LP or CaO. The NaOH and CaO improved silage degradability thus improving its quality. The use of LP in the present study did not benefit silage for energy-cane cultivars. The use of NaOH and CaO improves the quality of the silage, two varieties of sugarcane energy VG3 and VG1126, enabling its use with better efficiency for animal feed.

RESUMO: Objetivou-se estimar o desempenho fisiológico e produtivo de genótipos de batata em dois ambientes de cultivo em sistema orgânico. Foram conduzidos dois ensaios, sendo um em condições de ambiente protegido e outro em campo aberto. Empregou-se o delineamento em blocos completos casualizados com quatro repetições e cinco tratamentos referentes aos genótipos ‘Agata’, ‘Atlantic’, ‘BRS Ana’, ‘BRSIPR Bel’ e ‘BRS Clara’. Avaliaram-se atributos fisiológicos, produtivos e defeitos associados aos tubérculos. O ambiente protegido é o mais adequado para a produção de batata orgânica nas condições avaliadas. Os genótipos ‘BRSIPR Bel’ e ‘BRS Ana’ possuem alto potencial produtivo em condições de sistema orgânico.

ABSTRACT: The objective was to estimate the physiological and productive performance of potato genotypes in two organic cultivation systems. Two trials were conducted, being one in a protected and on in an open-field environment. A randomized complete block design with four replicates and five treatments (genotypes ‘Agata’, ‘Atlantic’, ‘BRS Ana’, ‘BRSIPR Bel’ and ‘BRS Clara’) was used. Physiological, productive characteristics and defects associated with tubers were obtained. The protected environment is the most appropriate to produce organic potatoes under the evaluated conditions. Genotypes ‘BRSIPR Bel’ and ‘BRS Ana’ exhibit a high productive potential in organic conditions.

ABSTRACT In Brazil, Anodontites trapesialis is a mussel species that occurs spontaneously in fish breeding sites. This species feeds on the remains of cultural treatments of other aquaculture species. The objective of the work is to understand the bromatological composition of mussel A. trapesialis. Individuals were selected to compose three samples of 500 g each of A. trapesialis for chemical composition, with viscera and viscera + shells being evaluated. The species presented 40.42 to 62.76 g of viscera per individual with a moisture content of 5.16% for viscera + shells and 86.5% for viscera. The mussel contains 42.6 and 12.96% crude protein, 3.16 and 1.76 of ether extract, 0.76 and 2.5% of crude fiber for viscera and viscera + shells. The total digestible nutrients were 74.16 and 36.96% for viscera and viscera + shells and 18.43 and 52.83% of mineral material for viscera and viscera + shells. A. trapesialis has relevant characteristics in its chemical composition as high protein content that gives the species potential for the human, animal, and other byproducts production.

Abstract The Amazon region has shown commercial potential for native mushroom species, such as mushrooms produced by the Yanomami people, who already sell more than 10 Amazon species. Among the species collected and consumed by the Yanomami people is Favolus brasiliensis (Fr.) Fr. (Basidiomycota: Polyporaceae), which occurs naturally in tropical areas of Central and South America. Thus, the objective of this work is to carry out the bromatological characterization of F. brasiliensis, contributing to a better understanding of the nutritional and food potential, as well as registering the natural occurrence of the species in the Cerrado biome and in the state of Goiás. The F. brasiliensis mushrooms collected in the present study showed an average of 7.4% humidity, 27% crude protein, 1.5% ether extract, 17% crude fiber, and 1.7% mineral matter. Compared with other species of edible mushrooms, both wild and cultivated, the moisture content of F. brasiliensis (93.60%) is low for species of the genus Pleurotus. For example, the humidity varies from 87% to more than 90%, similar to that found in Lentinus crinitus, another mushroom native to Brazil and also consumed by the Yanomami people. Thus, F. brasiliensis has the potential to be used in human foods.

RESUMEN El objetivo fue determinar el efecto de los sustratos comerciales y las fuentes orgánicas en la producción de plántulas de uchuva (Physalis peruviana L.). El diseño fue completamente aleatorizado, en un esquema factorial de 2×3, con dos sustratos comerciales la producción de plántulas (Carolina®, Bioplant®) y tres fuentes orgánicas (control, vermicompost enriquecido con termofosfato yoorin y estiércol de aves de corral). Agregar estiércol de aves de corral a sustratos comerciales causa efectos negativos sobre la precocidad y la emergencia. Carolina® es un sustrato adecuado para producir plántulas de uchuva sin necesidad de suplementos con fuentes orgánicas. Bioplant® se comporta más como un acondicionador de emergencia, que requiere suplementos con fuentes orgánicas (preferiblemente vermicompost). Las plántulas adecuadas para el trasplante se pueden obtener a los 47 días después de la siembra.

ABSTRACT The objective was to determine the effect of commercial substrates and organic sources on the production of cape gooseberry seedlings (Physalis peruviana L.). The design was completely randomized with a 2×3 factorial scheme using two commercial substrates (Carolina®, Bioplant®) and three organic sources (control, vermicompost enriched with yoorin thermophosphate and poultry manure) for seedling production. Adding poultry manure to commercial substrates negatively affects precocity and emergence. Carolina® is a suitable substrate for cape gooseberry seedling production without supplements using organic sources. Bioplant® behaves more like an emergence conditioner, requiring supplementation with organic sources (preferably vermicompost). Seedlings that are suitable for transplant can be obtained at 47 days after sowing.

Abstract Much of the diversity of mushrooms is still unknown to science. In the search for new edible mushrooms, we began to look at the traditions of Amazonian forest peoples in order to learn about the potential for edible mushrooms among other species. In particular, the Yanomami people launched the encyclopedia of food (Sanoma) in 2016 to preserve their knowledge of their own food. They demonstrated how food production can be balanced with the forest and present dozens of mushrooms that are consumed by their communities. In view of the above information, the objective of this work is to present bromatological aspects of the Lentinus crinitus mushroom from an agroforestry homegarden in the Cerrado. The specimen was collected from an agroforestry homegarden with biome forest species and agronomic species, located in the Brazilian Cerrado, in the city of Goiânia, Goiás. L. crinitus mushrooms presented 61% humidity, containing 14% crude protein, 1.5% ether extract, 26% crude fiber, and 3.4% mineral matter. Its bromatological characteristics are similar to others of commercial fungi. There is great potential for the use of the Lentinus crinitus mushroom in agroforestry yards in the Brazilian Cerrado, reconciling environmental conservation with the diversification of the production of agroextractive products.

Resumo Córregos de baixa ordem localizados perto de áreas urbanas geralmente recebem águas residuais domésticas, industriais e agrícolas que afetam negativamente a qualidade da água do rio. Além disso, a poluição da água está associada à variações do uso da terra ao redor do rio, caracterizada pela urbanização não planejada, atividades agrícolas intensas e desmatamento. O objetivo deste trabalho foi correlacionar o padrão de uso do solo com a qualidade físico-química e o potencial genotóxico da água em quatro pontos (P1 a P4) ao longo do rio Extrema, localizado em uma área industrial e agrícola do Brasil Central. Análises físico-químicas indicaram que a água coletada no rio Extrema é inadequada para consumo humano. Usando o modelo de Allium cepa, nenhuma evidência de citotoxicidade foi observada em nenhum momento; por outro lado, foi observado o potencial genotóxico dessas amostras de água. A correlação desses resultados com o uso do solo mostrou que a água coletada no P3 foi a mais contaminada; isso é provável devido ao influxo de águas residuais municipais e industriais e de águas residuais das atividades agrícolas. Resultados diferentes foram observados para P2 e P4, onde a análise do uso do solo atribuiu a qualidade da água às queimadas na floresta. Concluímos que diferentes usos do solo alteraram as características da água do rio e que os resultados obtidos pelo teste com A. cepa estavam mais relacionados às características do uso do solo do que aos parâmetros físico-químicos. Este estudo destaca a importância de associar o uso do solo ao potencial citogenotóxico da água.

Abstract Low-order streams located near urban areas usually receive domestic, industrial, and agricultural wastewaters that negatively affect river water quality. Additionally, water pollution is associated with land-use variations around the river, which is characterized by unplanned urbanization, intense agricultural activities, and deforestation. This work correlated land-use patterns with physicochemical quality and genotoxic potential of water at four points (P1 to P4) along the Extrema River, located in an industrial and agricultural area of Central Brazil. Physicochemical analyses indicated that the water collected from the Extrema River is inappropriate for human consumption. Using the Allium cepa model, no evidence of cytotoxicity was observed at any point; in contrast, the genotoxic potential of these water samples was observed. The correlation of these results with land use showed that the water collected at P3 was the most contaminated; this is probably due to the inflow of wastewater from municipal, industrial, and agriculture activities. Different results were observed for P2 and P4, where land-use analysis attributed the water quality to forest burns. We concluded that differential use of the land changed the characteristics of the associated river water, and A. cepa parameters were more related to land-use characteristics than to physicochemical parameters. This study highlighted the importance of associating land use with the cyto genotoxic potential of water.

Abstract The objective of this study was to analyze the effect of different calcium chloride concentrations on the post-harvest conservation of jabuticaba variety Pingo de Mel at different storage temperatures. The fruit were collected 30 days after the anthesis and subjected to immersions in calcium chloride solution at different concentrations (0%, 2%, 4%, and 6%) for 30 minutes at room temperature. Then, the fruit were dried and packed in polypropylene bags and incubated in a chamber at different temperatures (6 °C, 12 °C, and 25 °C). Samples were analyzed at time 0 and every two days, up to 12 days of storage. No effect of different calcium chloride concentrations was observed in the fruit characteristics at different storage temperatures over time. During storage, an increase of soluble and total pectin contents, antioxidant activity, CO2, total sugars, acidity, and weight loss was observed. Higher storage temperatures affected both the increase in weight loss, pH, and O2 production and the reduction of vitamin C content. Refrigeration was important for the post-harvest conservation of jabuticaba variety Pingo de Mel , once the fruit stored at 6 °C suffered minor variations with improvements or little changes during the 12 days.

RESUMEN El cultivo de Crambe abyssinica, es explotado comercialmente en Brasil, así la importancia de desarrollar estudios que posibiliten el perfeccionamiento de las técnicas involucradas en su producción. La plantación sobre restos culturales de diferentes especies de cobertura puede aumentar el desarrollo vegetativo y productivo del crambe. Así, el objetivo de este trabajo fue evaluar el desarrollo vegetativo y reproductivo del crambe cultivado sobre restos culturales de diferentes especies, en sucesión al cultivo de lechuga crespa. El delineamiento experimental utilizado fue en bloques al azar. Los tratamientos correspondieron a restos culturales de plantas de cobertura, siendo estas: crotalária (Crotalaria juncea), frijol guandu (Cajanus cajan), frijol de porco (Canavalia ensiformis), milheto (Pennisetum glaucum) y sorgo (Sorghum bicolor), además de un tratamiento control, con uso de vegetación espontánea. A los 49 días se evaluó altura de planta, diámetro de tallo, número de hojas, contenido relativo de clorofila, número de ramas laterales, masa seca y fresca de la parte aérea. Las variables productivas se evaluaron a los 95 días después de la siembra, obteniendo la masa de granos por planta, el peso hectolitro de los granos. Posteriormente fue estimada la productividad de cada tratamiento y el ingreso bruto. Se verificó que mantener los restos culturales de plantas de cobertura beneficia el desarrollo vegetativo de la cultura de crambe y no altera la productividad de los granos. Esta técnica aumenta el beneficio económico del cultivo, siendo recomendada durante el barbecho, en detrimento del manejo de plantas espontáneas.

ABSTRACT Although the crambe is commercially exploited in Brazil, there is a demand for studies that allow the improvement of the techniques involved in its cultivation. Planting on residues of different species can provide greater vegetative and reproductive development. Thus, the objective of this work was to evaluate the vegetative and reproductive development of crambe cultivated on residue of different species, in succession to the cultivation of lettuce. The experimental design was a randomized block design, with six treatments containing the following cover crops residues: crotalaria (Crotalaria juncea), pigeon pea (Cajanus cajan), porcine bean (Canavalia ensiformis), millet (Pennisetum glaucum) and sorghum (Sorghum bicolor), besides a control treatment, with the use of spontaneous vegetation. At 49 days the vegetative development was evaluated by measuring plant height, stem diameter at the soil level, number of leaves, relative content of leaf chlorophyll, number of lateral branches, fresh and dry biomass of shoot. The productive variables were evaluated at 95 days after planting, obtaining the grain mass per plant, the hectoliter weight of the grains and estimating the productivity of each treatment, as well as its gross revenue. It was verified that the maintenance of residue of cover crops benefits the vegetative development of the crambe crop, without statistically altering grain yield. However, this technique results in an economic benefit, with the increase in gross revenue, being recommended during the fallow, to the detriment of the management of spontaneous plants.

RESUMEN Chloridea (Heliothis) subflexa (Guenée) (Lepidoptera: Noctuidae: Heliothinae) es una especie frugívora y monófoga con hospederos que consta de las plantas del género Physalis. En los últimos años, gusano del tomato de cascara se convirtió en la principal plagas-insectos en cultivos de tomatillo (Physalis ixocarpa Brotero de México) y cultivo de la uchuva (Physalis peruviana L.) en Argentina. El objetivo fue reportar el primer registro de C. subflexa en P. peruviana en Brasil. Los especímenes se recogieron en la fase larvaria y se mantuvieron en una dieta natural hasta llegar a la etapa adulta. La identificación de especies se realizó en base a criterios morfológicos. Se registraron la presencia y daños causados por C. subflexa en frutos de P. peruviana, producidos en un sistema orgánico. Este es el primer registro para Brasil.

ABSTRACT Chloridea (Heliothis) subflexa (Guenée) (Lepidoptera: Noctuidae: Heliothinae) is a frugivorous and monophagous species with plant hosts in the genus Physalis. In the last few years, the subflexus straw moth became the main pest-insect in tomatillo crops (Physalis ixocarpa Brotero) in Mexico and cape gooseberry (Physalis peruviana L.) crops in Argentina. The objective was to provide the first record of C. subflexa on P. peruviana in Brazil. The specimens were collected in the larval stage and kept on a natural diet until reaching the adult stage. Species identification was carried out based on morphological criteria. Damage from C. subflexa on P. peruviana fruits cultivated in an organic system were recorded. This is the first record in Brazil.