Abstract Intratumoral similarities and differences between large-cell neuroendocrine carcinomas (LCNECs) and small-cell lung carcinomas (SCLCs) are determined partially by the Notch signaling pathway, which controls the switch from neuroendocrine to slight/non-neuroendocrine cell fate. LCNECs are divided into two subgroups according to genomic alterations: type I LCNECs exhibit a neuroendocrine profile characterized by achaete‐scute homolog 1 (ASCL1)high/delta-like protein 3 (DLL3)high/NOTCHlow and type II LCNECs show the pattern ASCL1low/DLL3low/NOTCHhigh. Here, we used immunohistochemistry, transmission electron microscopy, and digital analysis to examine the role of the Notch ligand DLL3 as an immunomarker of the neuroendocrine state and ASCL1 as a regulator of cell-cell interactions in SCLCs and LCNECs. High DLL3 and ASCL1 expression was associated with atypical submicroscopic characteristics involving nuclear size, chromatin arrangement, Golgi apparatus, and endoplasmic reticulum, and was characteristic of type I LCNECs with similarity to SCLCs, whereas low DLL3 and ASCL1 expression was found in both SCLCs and type II LCNECs. In patients diagnosed at an early stage who did not have metastasis and who underwent chemotherapy, DLL3high and ASCL1high SCLCs and type I LCNECs were associated with a better prognosis and a lower risk of death. The present findings suggested that DLL3/ASCL1 are potential therapeutic targets and prognostic indicators in patients with SCLCs or LCNECs. largecell large (LCNECs smallcell small (SCLCs pathway slight/nonneuroendocrine slightnonneuroendocrine slight/non slight non fate alterations achaetescute achaete scute ASCL1high/deltalike ASCL1highdeltalike ASCLhighdeltalike high/delta like high delta ASCL DLL3high/NOTCHlow DLL3highNOTCHlow DLLhighNOTCHlow high/NOTCHlow NOTCHlow DLL ASCL1lowDLL3lowNOTCHhigh ASCLlowDLLlowNOTCHhigh ASCL1low DLL3low NOTCHhigh ASCL1low/DLL3low/NOTCHhigh Here immunohistochemistry microscopy cellcell size arrangement apparatus reticulum chemotherapy DLLhigh ASCLhigh death DLL3ASCL1 DLLASCL DLL3/ASCL nonneuroendocrine slightnon deltalike highdeltalike highdelta highNOTCHlow lowDLL lowNOTCHhigh ASCLlow DLLlow DLL3ASCL

Foram realizados dois ensaios nos quais compararam-se plantas de girassol, do cultivar Anhandy, livremente visitadas por insetos(testemunhas) e outras impedidas de visitação por dois métodos (cobertura de plantas com gaiolas teladas, de 3m de largura, 4m de comprimento e 2m de altura, e ensacamento de capítulos com filó). O número, o peso e a porcentagem de sementes granadas foram significativamente maiores (P<0,05) nas plantas visitadas (579,28; 41,17g e 82,37%; 456,97; 23,44g 79,70%, nos dois ensaios), em relação às plantas cobertas com gaiolas teladas (81,53; 2,23g e 1,16% no primeiro ensaio e 111,92; 3,16g e 28,49%, no segundo). O número e o peso de sementes não granadas foram significativamente maiores (P<0,05) nas plantas cobertas com gaiolas teladas (416,29 e 6,58g; 375,25 e 5,04g, nos dois ensaios) em relação à testemunha (142,69 e 1,71g; 182,38 e 2,53g). Os capítulos ensacados com filó apresentaram valores de porcentagem de sementes granadas intermediários entre as plantas testemunhas e as cobertas com gaiolas teladas (64,56 no primeiro ensaio e 68,61, no segundo). Pode-se concluir que o cultivar de girassol em estudo apresenta significativo aumento de produção de sementes devido a visitas de insetos polinizadores, principalmente abelhas, e que as gaiolas teladas são mais indicadas como método para impedir o acesso de insetos polinizadores.

Sunflower plants, cv Anhandy, freely visited by insects (control) were compared to plants protected from insects. Two flower isolation methods were used: covering the plants with net cages (width 3m, lenght 4m, height 2m) and bagging the flowers with gauze. The number, the weight and the percentage of seed setting were significantly higher (P<0.05) in the visited plants (579.28; 41.17g and 82.37%; 456.97; 23.44g 79.70%, in two trials) than in plants protected by cages (81.53; 2.23g and 1.16% in the first trial and 111.92; 3.16g e 28.49%, in the second). The number and the weight of no seed settings were significantly higher (P<0.05) in the plants covered with cages (416.29 and 6.58g; 375.25 and 5.04g in two trials) than in the control (142.69 and 1.71g; 182.38 and 2.53g). The percentage of seed setting in the bagged flowers with gauze was intermediate to the control plants and the covered plants. (64.56% in the first trial and 68.61%, in the second). It is concluded that the sunflower, cv Anhandy, presents a significant increase of seed production due to visits of pollinating insects, mainly honey bees. Cage is the most indicated method to protect plants from pollinating insects.