Aim This study aimed to evaluate how different study designs influences the presence and characteristics of diagnosis of oral manifestations in patients with COVID-19. Methods This study is a meta-search conducted with studies evaluating the relationship between patients with oral manifestations and COVID-19. A search was conducted in three databases (PubMed, Web of Science, and Scopus), using terms specific to COVID-19 and oral lesions, including only primary studies. Study selection was undertaken by two reviewers independently. A descriptive analysis was performed. Results The results included 55 studies in the data synthesis analysis. Most of the studies were case reports and case series. Some of the studies did not relate a biological test to confirm COVID-19. The ulcer lesion and that clinical examination by specialists were the most reported lesion in case reports and case series studies, while taste alterations and patient self-report were more reported in observational studies. Conclusions This review concluded that the oral lesions in patients with COVID-19 differ according to the study design. The lack of high-quality scientific evidence in the dental literature on COVID-19 makes the findings inconsistent by the lack of sample selection criteria, diagnostic criteria and classification of oral lesions. COVID19. COVID19 COVID 19. 19 metasearch meta PubMed, PubMed (PubMed Science Scopus, Scopus , Scopus) COVID-1 independently performed 5 selfreport self report design highquality high quality COVID1 1 COVID-

Resumo O objetivo deste estudo foi avaliar a resistência à fadiga de molares sem um remanescente coronário, utilizando zero, um, dois ou três pinos de fibra de vidro. Quarenta molares humanos livres de cárie e fissuras, com porções coronárias removidas ao nível do assoalho da câmara pulpar, foram alocados aleatoriamente em quatro grupos (n=10). Após o tratamento endodôntico, os pinos (Whitepost DC/DC.E 0.5, 1.0, FGM) foram silanizados (agente silano, Angelus) e cimentados com um cimento resinoso autoadesivo (RelyX U200, 3M ESPE). Núcleos de resina composta (Z350, 3M ESPE) foram construídos, coroas metálicas foram fabricadas e cimentadas usando o mesmo sistema de cimentação. As amostras foram submetidas a testes de fadiga cíclica a 45°, aplicando carga na superfície oclusal a 10 Hz e carga inicial de 100 N por 10.000 ciclos, com incrementos de 50 N a cada 10.000 ciclos até a ocorrência da falha. Os dados de carga de falha por fadiga e ciclos até a falha foram registrados e submetidos à análise de sobrevivência por meio de testes post hoc de Kaplan-Meier e Mantel-Cox, além de análise de Weibull. Os padrões de fratura das coroas que falharam foram analisados qualitativamente. O grupo sem pinos apresentou o desempenho mais baixo em fadiga (p < 0,05) tanto para carga de falha por fadiga quanto para ciclos até a falha. Um desempenho superior em fadiga foi observado no grupo com três pinos, seguido pelos grupos com um ou dois pinos, corroborado pelo parâmetro de resistência característica de Weibull. Os módulos de Weibull foram semelhantes entre as condições. Todas as amostras apresentaram falha envolvendo desprendimento do conjunto restaurador (pinos/núcleo/coroa) com uma porção do remanescente dentário, sem fratura do dente. Portanto, ao restaurar molares mandibulares sem remanescente coronário, o uso de pinos de fibra de vidro promove uma maior resistência à fadiga a cargas oblíquas. coronário zero fissuras pulpar n=10. n10 n n=10 . (n=10) endodôntico Whitepost DCDCE DC E 05 0 5 0.5 1 1.0 FGM agente silano Angelus RelyX U200 U M ESPE. ESPE Z350, Z350 Z (Z350 construídos cimentação 45 45° 10000 000 10.00 KaplanMeier Kaplan Meier MantelCox, MantelCox Mantel Cox, Cox Mantel-Cox qualitativamente p 0,05 005 condições pinos/núcleo/coroa pinosnúcleocoroa núcleo coroa (pinos/núcleo/coroa dentário dente Portanto oblíquas n1 n=1 (n=10 0. 1. U20 Z35 (Z35 4 1000 00 10.0 0,0 n= (n=1 U2 Z3 (Z3 10. 0, (n= (Z (n

Abstract This study aimed to assess the fatigue resistance of molars lacking a coronary remnant, using zero, one, two, or three fiberglass posts. Forty caries/crack-free human molars with coronal portions removed at the pulp chamber floor were randomly allocated into four groups (n=10). Following endodontic treatment, posts (Whitepost DC/DC.E 0.5, 1.0, FGM) were silanized (silane agent, Angelus) and cemented with a resin cement (RelyX U200, 3M ESPE). Resin composite cores (Z350, 3M ESPE) were built, and metal crowns were fabricated and cemented using the same luting system. Samples underwent cyclic fatigue testing at 45°, applying load in the occlusal surface at 10 Hz and 100 N initial load for 10,000 cycles, with 50 N increments every 10,000 cycles until failure. Fatigue failure load and cycles for failure data were recorded and subjected to survival analysis through Kaplan-Meier and Mantel-Cox post hoc tests, and Weibull analysis. Fractography patterns of failed crowns were qualitatively analyzed. The group without posts exhibited the lowest fatigue performance (p < 0.05) for both fatigue failure load and cycles to failure. Superior fatigue performance was observed in the three-post group, followed by groups with one or two posts, corroborated by the Weibull characteristic strength parameter. Weibull moduli were similar among conditions. All specimens exhibited failure involving detachment of the restorative set (posts/core/crown) with a portion of the dental remnant, without tooth fracture. Thus, when restoring mandibular molars without crown remnants, the use of fiberglass posts promotes greater fatigue resistance to oblique loads. remnant zero caries/crackfree cariescrackfree caries/crack free caries crack n=10. n10 n n=10 . (n=10) treatment Whitepost DCDCE DC E 05 0 5 0.5 1 1.0 FGM silane agent Angelus RelyX U200 U M ESPE. ESPE Z350, Z350 Z (Z350 built system 45 45° 10000 000 10,00 KaplanMeier Kaplan Meier MantelCox Mantel Cox tests analyzed p 0.05 005 threepost parameter conditions posts/core/crown postscorecrown core (posts/core/crown fracture Thus remnants loads crackfree cariescrack n1 n=1 (n=10 0. 1. U20 Z35 (Z35 4 1000 00 10,0 0.0 n= (n=1 U2 Z3 (Z3 10, (n= (Z (n

Resumo Esta meta-pesquisa buscou avaliar a conduta, o relato e as principais características de ensaios clínicos randomizados (ECRs) publicados na odontologia. Foi realizada uma busca no PubMed por ECRs em odontologia indexados de 31 de dezembro de 2016 a 31 de dezembro de 2021. Dois revisores avaliaram independentemente os estudos quanto à presença dos critérios de elegibilidade. Apenas estudos em inglês foram considerados. Dados do periódico e do autor, especialidade, métricas de citação e publicação, detalhes de relato e conduta dos estudos foram coletados. Uma análise descritiva dos dados, um mapa mostrando o número de ECRs por país e um gráfico de rede da colaboração científica entre diferentes países foram apresentados. Incluímos 844 artigos. A principal área de pesquisa foi a periodontia (16,35%). O maior número de ECRs foi atribuído ao Brasil (16,59%). Autores dos EUA estabeleceram a maioria das conexões com outros países. A maioria dos estudos não relatou o uso do CONSORT (67,89%), o tipo de randomização (69,31%) ou o mecanismo usado para implementar a sequência de alocação randomizada (52,37%). No entanto, a maioria dos estudos incluiu "randomized" no título (71,92%) e relatou o método para gerar uma sequência de alocação randomizada (65,88%) e o cegamento (74,88%). Os ECRs são a base para a tomada de decisões clínicas. Nossos resultados fornecem uma visão mais clara dos ECRs atuais e identificam áreas que requerem melhorias. O Brasil foi o país que mais produziu ECRs, e os EUA foram o principal colaborador. Ressaltamos a variabilidade das características de relato e conduta do estudo. metapesquisa meta (ECRs 3 201 2021 elegibilidade considerados autor especialidade publicação coletados dados apresentados 84 artigos 16,35%. 1635 16,35% . 16 35 (16,35%) 16,59%. 1659 16,59% 59 (16,59%) 67,89%, 6789 67,89% , 67 89 (67,89%) 69,31% 6931 69 (69,31% 52,37%. 5237 52,37% 52 37 (52,37%) entanto randomized "randomized 71,92% 7192 71 92 (71,92% 65,88% 6588 65 88 (65,88% 74,88%. 7488 74,88% 74 (74,88%) clínicas melhorias colaborador estudo 20 202 8 163 16,35 1 (16,35% 165 16,59 5 (16,59% 678 67,89 6 (67,89% 69,31 693 (69,31 523 52,37 (52,37% 71,92 719 7 9 (71,92 65,88 658 (65,88 748 74,88 (74,88% 2 16,3 (16,35 16,5 (16,59 67,8 (67,89 69,3 (69,3 52,3 (52,37 71,9 (71,9 65,8 (65,8 74,8 (74,88 16, (16,3 (16,5 67, (67,8 69, (69, 52, (52,3 71, (71, 65, (65, 74, (74,8 (16, (67, (69 (52, (71 (65 (74, (16 (67 (6 (52 (7 (74 (1 ( (5

Abstract This meta-research sought to evaluate the conduct, reporting, and main characteristics of published randomized controlled trials (RCTs) in dentistry. A search was performed on PubMed for RCTs in dentistry indexed from 31 December 2016 to 31 December 2021. Two reviewers independently screened the studies for the presence of eligibility criteria. Only studies in English were considered. Journal and author data, subject, citation and publishing metrics, reporting, and details of study conduct were collected. A descriptive analysis of the data, a map depicting the number of RCTs per country, and a network graph of scientific collaboration among different countries were presented. We included 844 articles. The main research area was periodontics (16.35%). The highest number of RCTs was attributed to Brazil (16.59%). Authors from the USA established the most links with other countries. Most studies did not report the use of CONSORT (67.89%), the type of randomization (69.31%), or the mechanism used to implement the random allocation sequence (52.37%). However, most studies included “randomized” in the title (71.92%) and reported the method for generating a random allocation sequence (65.88%) and blinding (74.88%). RCTs are the basis for clinical decision-making. Our results provide a better view of current RCTs and identify areas that require improvement. Brazil was the country that produced the most RCTs, and the USA was the main collaborator. We emphasize the variability of reporting characteristics and study conduct. metaresearch meta (RCTs 3 201 2021 criteria considered data subject metrics collected presented 84 articles 16.35%. 1635 16.35% . 16 35 (16.35%) 16.59%. 1659 16.59% 59 (16.59%) 67.89%, 6789 67.89% , 67 89 (67.89%) 69.31%, 6931 69.31% 69 (69.31%) 52.37%. 5237 52.37% 52 37 (52.37%) However “randomized 71.92% 7192 71 92 (71.92% 65.88% 6588 65 88 (65.88% 74.88%. 7488 74.88% 74 (74.88%) decisionmaking. decisionmaking decision making. making decision-making improvement collaborator 20 202 8 163 16.35 1 (16.35% 165 16.59 5 (16.59% 678 67.89 6 (67.89% 693 69.31 (69.31% 523 52.37 (52.37% 71.92 719 7 9 (71.92 65.88 658 (65.88 748 74.88 (74.88% 2 16.3 (16.35 16.5 (16.59 67.8 (67.89 69.3 (69.31 52.3 (52.37 71.9 (71.9 65.8 (65.8 74.8 (74.88 16. (16.3 (16.5 67. (67.8 69. (69.3 52. (52.3 71. (71. 65. (65. 74. (74.8 (16. (67. (69. (52. (71 (65 (74. (16 (67 (69 (52 (7 (6 (74 (1 (5 (

Resumo O objetivo do estudo foi mapear, através de uma revisão de escopo, as evidências disponíveis na literatura sobre o uso de agregrantes plaquetários em pacientes comprometidos e que realizaram cirurgias odontológicas. Pesquisas foram realizadas em bases de dados por estudos clínicos com pacientes comprometidos que realizaram cirurgia odontológica e usaram agragantes plaquetários. Apenas estudos em inglês foram incluídos. Dois pesquisadores independentes realizaram a seleção dos estudos. Os seguintes dados foram extraídos: desenho do estudo, objetivo, procedimento cirúrgico, agregante plaquetário usado, envolvimento sistêmico, desfecho analisado e principais resultados. Uma análise descritiva dos dados foi realizada. Vinte e dois estudos preencheram os critérios de elegibilidade e foram incluídos. Série de casos foi o desenho de estudo mais frequente entre os estudos incluídos (41,0%). Em relação ao comprometimento sistêmico, 19 estudos reportaram pacientes com câncer e em relação ao tratamento cirúrgico, 16 estudos reportaram pacientes que realizavam tratamento para osteonecrose relacionada ao uso de medicamentos. O agregante mais utilizado foi o plasma rico em fribina (P-PRF). Em geral, maioria dos estudos recomendou o uso dos agregantes plaquetários. Assim, os resultados desse estudo sugerem que a evidência relacionada ao uso de agregantes plaquetários em pacientes comprometidos que realizam cirurgia odontológica é ainda inicial. Ainda, a maioria dos estudos avaliaram o uso de agregantes plaquetários em pacientes com osteonecrose. mapear escopo odontológicas extraídos cirúrgico usado sistêmico realizada 41,0%. 410 41,0% . 41 0 (41,0%) 1 medicamentos PPRF. PPRF P PRF (P-PRF) geral Assim inicial Ainda 41,0 4 (41,0% (P-PRF 41, (41,0 (41, (41 (4 (

Abstract The objective of this study was to map, through a scoping review, the evidence available in the literature on the use of platelet concentrates in compromised patients undergoing oral surgeries. Searches were performed in electronic databases for clinical studies with compromised patients undergoing oral surgery who used platelet concentrates. Only studies published in English were included. Two independent researchers carried out the selection of studies. The study design and objective, surgical procedure and platelet concentrate used, systemic involvement, analyzed outcome, and main results were extracted. A descriptive analysis of the data was performed. Twenty-two studies met the eligibility criteria and were included. Case series was the most frequent study design among the included studies (41.0%). In terms of systemic disability, 19 studies reported patients with cancer and related to surgical treatment 16 studies reported patients underwent treatment for osteonecrosis related to the use of the drug. The most used platelet concentrate was pure platelet-rich fibrin (P-PRF). In general, most studies recommend the use of platelet concentrates. Thus, the results of this study suggest that the evidence related to the use of platelet concentrates in compromised patients when undergoing oral surgeries is still initial. Also, most studies assessed the use of platelet concentrates in patients with osteonecrosis. map review involvement outcome extracted Twentytwo Twenty two 41.0%. 410 41.0% . 41 0 (41.0%) disability 1 drug plateletrich rich PPRF. PPRF P PRF (P-PRF) general Thus initial Also 41.0 4 (41.0% (P-PRF 41. (41.0 (41. (41 (4 (

Aim This study aimed to assess the reporting characteristics of systematic review abstracts published in the proceedings of the Sociedade Brasileira de Pesquisa Odontológica (SBPqO) meeting. Methods We selected abstracts published in the SBPqO meeting proceedings of 2019 and 2020, mentioning that a systematic review was conducted in the title, objective or methods sections. One researcher performed the screening and the data extraction after a pilot test training. The following data were extracted: affiliation of the primary author, dental specialization, the term “systematic review” mentioned in the title, reporting of the objective, reporting of eligibility criteria, reporting of information sources, reporting of the number of included studies and if a meta-analysis was performed. A descriptive analysis of the data was performed with data summarized as frequencies. Results We included 235 abstracts. A total of 20 studies were from the Universidade de Uberlândia (8.5%), and the main specialization was Restorative and Esthetic Dentistry, with 47 studies (20%). Most of the studies mentioned the term “systematic review” in the title (n=219; 93.2%) and reported the objective (n=231; 98.3%). A great majority of studies did not report the eligibility criteria (n=97; 41.3%) or it was classified as unclear (n=96; 40.8%). The great majority of studies only reported the databases searched (n=103; 43.8%) or databases and date of search (n=74; 31.5%). Most of the studies reported the number of included studies (n=204; 86.8%). Conclusion Based on this study, the reporting characteristics of systematic review abstracts published in the proceedings of the SBPqO meeting are satisfactory. However, there is room for improvement.

Resumo O efeito da técnica de preparo do canal radicular na iniciação e/ou propagação de microfissuras dentinárias é um tema controverso. Essa revisão sistemática teve como objetivo avaliar o papel das técnicas de preparo do canal radicular com diferentes cinemáticas (manual, rotatória, reciprocante, adaptativa e self-adjusting file) na iniciação e/ou propagação de microfissuras dentinárias. Estudos in vitro e in situ comparando a influência de pelo menos duas técnicas diferentes de preparo do canal radicular foram identificados no PubMed/MEDLINE e SCOPUS até 05 de junho de 2018 sem restrição de idioma e tempo. Dois autores revisaram de maneira independente todos títulos e resumos para elegibilidade dos estudos. Tabelas foram criadas para sumarizar os estudos incluídos e os estudos foram avaliados quanto ao risco de viés. Cinquenta e quatro (54) estudos enquadraram-se nos critérios de elegibilidade. Os resultados foram classificados de acordo com o método utilizado para avaliação das microfissuras. A maioria dos estudos que utilizaram micro-tomografia não demonstraram formação de novas microfissuras após preparo do canal radicular. Em geral, técnicas de instrumentação induziram formação de microfissuras quando os métodos de avaliação foram destrutivos, independente da cinemática. Em relação a região do ápice, quando comprimento de trabalho foi definido como o comprimento do canal menos 1 mm, o risco de indução de microfissuras é reduzido. A maioria dos estudos incluídos apresentaram baixo risco de viés para todos os domínios apresentados. Nossos resultados parecem indicar que as diferentes técnicas de preparo do canal radicular não causam danos a estrutura dental quando adequadamente utilizadas e a metodologia adequada é utilizada.

Abstract The effect of root canal preparation technique on microcrack initiation is a controversial issue. This systematic review aimed to assess the role of root canal preparation techniques with different kinematics (manual, rotary, reciprocating, adaptive, self-adjusting file) on microcrack initiation. In vitro and in situ studies comparing the influence of at least two different root canal preparation techniques on the initiation of dentin microcracks were searched in PubMed/MEDLINE and SCOPUS up to June 5, 2018 without language and period restriction. Two authors independently reviewed all identified titles and abstracts for eligibility. Tables were generated to summarize the included studies, and the included studies were assessed for bias. Fifty-four (n=54) articles met the eligibility criteria. The results were classified according to the method used for microcrack evaluation, and most studies that used micro-computed tomography showed no formation of new cracks after root canal preparation. In general, the instrumentation techniques induced microcrack formation when the methods were destructive, irrespective of kinematics. In relation to the apex region, when the preparation working length was set as the root canal length subtracted of 1 mm, the risk of microcrack initiation reduces. The majority of the included studies had low risk of bias for all assessed domains. Our results seem to indicate that the various root canal preparation techniques considered in this study will not cause damage to the dental structure when adequately employed and the proper methodology is applied.

Resumo Dois casos clínicos são apresentados para explorar as diferenças técnicas e discutir as vantagens e desvantagens de se utilizar zircônia com cerâmica estratificada ou monolítica para confeccionar coroas unitárias. O primeiro caso descreve os passos clínicos na confecção de uma coroa monolítica no segundo pré-molar esquerdo utilizando uma zircônia altamente translúcida. Neste caso é apresentado a confecção de um preparo conservador devido as propriedades mecânicas deste material bem como suas características ópticas encontradas com a pigmentação e maquiagem. No segundo caso, um tratamento restaurador convencional de estratificação foi realizado utilizando uma infraestrutura de zircônia seguido pelo recobrimento com cerâmica feldspática em um primeiro molar inferior esquerdo. A literatura recente indica que cada uma dessas alternativas restauradoras apresenta vantagens e desvantagens específicas. Fatores como desempenho mecânico, fratura, características estéticas, sucesso clínico, taxas de complicações, adesão e desgaste do antagonista são discutidos comparando as duas possibilidades restauradoras. Os achados da literatura destacam que coroas monolíticas impedem um grande problema relatado em restaurações estratificadas: o lascamento da cerâmica de recobrimento. Coroas monolíticas também permitem preparos dentários minimamente invasivos, aumentando assim a preservação do remanescente dentário. No entanto, dados suportando um desempenho estético similar entre coroas monolíticas e estratificadas são inexistentes, assim a previsibilidade de uso é restrita para a região posterior, pois casos exigindo alta demanda estética restaurações estratificadas continuam ser principalmente utilizadas. Falhas não foram encontradas e a satisfação do paciente foi relatada em ambas as técnicas após o acompanhamento de 12 meses.

Abstract Two clinical cases are presented to explore technical differences and discusses the advantages and disadvantages of using veneered or monolithic zirconia to manufacture posterior single crowns. The first case describes the clinical steps in manufacturing a monolithic crown on a mandibular left second premolar using high translucency zirconia. It shows the use of a conservative tooth preparation based on the superior mechanical properties that this material presents as well as the final optical characteristics achieved by shading and staining. In the second case, a conventional bilayer restorative treatment was made using zirconia framework followed by veneering with feldspar ceramic on a mandibular left first molar. Recent literature indicates that each of these restorative alternatives presents specific advantages and disadvantages. Factors such as mechanical performance, fracture, esthetic characteristics, clinical success, complication rates, adhesion and antagonist wear performance are discussed comparing the two restorative assemblies. The data highlight that monolithic crowns prevent a major problem reported on bilayer restorations: the chipping of veneering ceramic. Monolithic crowns also allow minimally invasive tooth preparations, thus increasing tooth remnant preservation. However, data that support esthetic performance similarity between monolithic and bilayer assemblies are lacking, thus the predictability of use is restricted for the posterior region, as cases demanding high esthetic appeal continue to fundamentally use bilayer restorations. Failures were not found, and patient satisfaction was reported in both techniques after the 12-month follow up.

Resumo O uso de guias de reporte tem um papel importante no desenvolvimento das pesquisas na saúde, melhorando a qualidade e a precisão das publicações. Esse estudo avaliou como periódicos de odontologia usam os guias de reporte. Todos editores de periódicos de odontologia registrados na lista do 2013 Journal of Citation Reports (n=81) foram convidados a participar. Dados foram coletados através de um questionário online autoaplicável. Informações sobre o perfil do periódico/editor e do uso de guias de reporte pelos periódicos foram coletados. Informações/recomendações sobre o uso de guias de reporte foram também coletados dos sites de todos os periódicos. Dados foram analisados descritivamente e frequências foram sumarizadas. Trinta e quatro (42%) editores completaram o questionário. Maioria dos periódicos é membro do Committee on Publication Ethics (64,7%) e/ou seguem as recomendações do International Committee of Medical Journal Editors (20,6%), enquanto 26,5% dos periódicos não são membros de nenhum grupo editorial. A maioria dos editores não é familiar com o EQUATOR Network (55,9%), não trabalha em tempo integral como editor (85,3%) e 88,2% recebem algum pagamento. A maioria deles recebeu treinamento para a posição de editor (55,9%). O CONSORT Statement foi endossado por 61,8% dos periódicos. Informações oriundas dos sites demonstraram que 44,4% dos periódicos não recomendam nenhum guia de reporte, 51,9% mencionam o CONSORT Statement no site e 28,4 apenas recomenda o CONSORT Statement. Existe um espaço claro para melhora no uso de guias de reporte em periódicos de odontologia. Um maior entendimento e endosso/aderência/implementação de guias de reporte por periódicos de odontologia pode promover a qualidade e transparência das pesquisas odontológicas publicadas.

Abstract The use of reporting guidelines has an important role in the development of health research, improving the quality and precision of the publications. This study evaluated how dental journals use reporting guidelines. All editors of dental journals registered on the 2013 Journal Citation Reports list (n=81) were invited to participate. The data were collected by a self-reported web-based questionnaire. Information about the profile of journal/editor and on the use of reporting guidelines by journals was gathered. Information/recommendations about the use of reporting guidelines were collected from the websites of all journals. Data were descriptively analyzed and frequencies were summarized. Thirty-four (42%) editors completed the questionnaire. Most journals are members of Committee on Publication Ethics (64.7%) and/or follow the International Committee of Medical Journal Editors recommendations (20.6%), while 26.5% are not members of any editorial group. Most editors are unfamiliar with the EQUATOR Network (55.9%), do not work full time (85.3%) and 88.2% have some income/payment. Most of them received educational training for this position (55.9%). The CONSORT Statement was endorsed by 61.8% of journals. Information from websites showed that 44.4% journals do not recommend any reporting guideline, 51.9% mention CONSORT Statement in the website and 28.4% only recommend the use of CONSORT Statement. There is clearly room for improving the use of reporting guidelines in dental journals. Broadening the understanding and the endorsement/adherence/implementation of reporting guidelines by journals may promote quality and transparence of published dental research.

Abstract: The objective of this study was to realize a scoping review the literature in order to identify the profile of DPSCs isolation and analyze the possible risk factors that could change the native behavior of these cells. An initial search was conducted using the following MeSH terms: “(dental pulp stem cell [MeSH])”; “(dental pulp [MeSH])” AND “(stem cell [MeSH])”; “(“dental pulp stem cell” [MeSH]”)”. The electronic search was done without date restriction up to and including April 2014, in PubMed, Scopus, Scielo and ISI Web of Knowledge databases. Studies were submitted to inclusion and exclusion criteria and 222 articles were included. Data showed that over the past 15 years many studies have been conducted using DPSCs. However this is the first systematic review regarding the isolation of stem cell, and more specifically of dental pulp stem cells. The isolation of dental pulp stem cells showed great variability, hampering the development of standard protocols to achieve in vitro dental pulp stem cells with similar characteristics. This scoping review combined, for the first time, the methodologies used for dental pulp stem isolation, highlighting the most frequently used.

The aim of this study was to evaluate if clinical experience, whether in relation to length of practice time and/or level of specialization influences the dentist’s preference for using posts to restore endodontically treated teeth. A cross sectional study was carried out using a questionnaire with dentists (n = 276) in Pelotas, southern Brazil. Data were collected regarding clinical experience, post-graduate training, and variables related to restorations (posts/cements and use of rubber dam) for endodontically treated teeth. The data were submitted to a descriptive analysis and associations were tested. The response rate was 68%. Cast metal posts (24.53%), glass fiber posts (20.75%) and resin cement (66.67%) were the most commonly selected materials. In relation to rubber dams, 93.05% of the dentists were found not use them to lute posts. There was a significant association between the level of training of post-graduate dentists and the type of post used (p = 0.027), in that dentists without post-graduate training used cast metal posts more frequently, whereas dentists with post-graduate training reported glass fiber posts as their first choice. The results of the study showed that dentists preferred cast metal posts, glass fiber posts and resin cement. Continuing education influenced the decision of the dentists on their choice of dental posts.

This study aimed to identify factors that can affect the retention of glass fiber posts to intra-radicular dentin based on in vitro studies that compared the bond strength (BS) of GFPs cemented with resin cements. Searches were carried out in PubMed and Scopus until December 2013. Bond strength values and variables as type of tooth, presence of endodontic treatment, pretreatment of the post, type of bonding agent (if present), type of cement and mode of cement application were extracted from the 34 included studies. A linear regression model was used to evaluate the influence of these parameters on BS. The presence of endodontic treatment decreased the BS values in 22.7% considering the pooled data (p = 0.013). For regular cement, cleaning the post increased BS when compared to silane application without cleaning (p = 0.032), considering cleaning as ethanol, air abrasion, or phosphoric acid application. Applying the cement around the post and into root canal decreased the resistance compared to only around the post (p = 0.02) or only into root canal (p = 0.041), on the other hand, no difference was found for self-adhesive resin cement for the same comparisons (p = 0.858 and p = 0.067). Endodontic treatment, method of cement application, and post pretreatment are factors that might significantly affect the retention of glass-fiber posts into root canals mainly when cemented with regular resin cement. Self-adhesive resin cements were found to be less technique-sensitive to luting procedures as compared with regular resin cements.

Different microbial identification methods have shown that the microbial community profiles in endodontic infections are diverse and assorted. The aim of this study was to evaluate the frequency of selected endodontic pathogens in the pulp chambers (PCs) and root canals (RCs) of infected primary teeth using PCR methods. Paired PC and RC samples were collected from 15 subjects and analyzed by PCR for the presence of Filifactor alocis, Fusobacterium nucleatum, Parvimonas micra, Porphyromonas endodontalis, Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens, Prevotella tannerae, Tanerella forsythia, Treponema denticola, and Treponema socranskii. The frequency of each species was determined in the PC and RC of each case. The species most frequently detected in PCs were P. nigrescens (86.7%), P. gingivalis (73.3%), and F. alocis (73.3%). Of the PC samples, 13.3% contained P. micra and T. denticola, and 6.7% contained T. forsythia. The species most frequently detected in RCs were P. gingivalis (100%) and P. nigrescens (93.3%). P. tannerae, P. micra, and T. denticola were found in 40% of the RC samples; T. forsythia was found in 26.7% of the RC samples. The “red complex”, which comprises P. gingivalis, T. denticola, and T. forsythia, was not found in the PC of any tooth but was found in 30% of the RC samples. The detection of P. nigrescens in the PC was statistically associated with the presence of P. nigrescens in the RC (p = 0.04). The results suggest high heterogeneity among the samples, even among those from the same subject.