Connaraceae is a plant family usually used in traditional medicine. Recently, we have reported the chemical composition and biological activity of 39 species around the world. This work highlights the application of these species to diabetes control, which has been reported at least to half of this family. In this line, there are several species of the Connaraceae without scientific evidence of traditional use, among these the four species study here. Thus, this work aims the chemical composition prospection of Connarus blanchetii, Connarus regnellii, Connarus suberosus and Rourea glazioui, as well as the identification of possible bioactivity. The leaves of these four species were collected, prepared and submitted to different extractive methods (aqueous decoction and infusion and ethanolic maceration), these being tested in a preliminary phytochemical investigation and subjected to antioxidant assay with 2,2-diphenyl-1-picrylhydrazyl and ferric reducing antioxidant power. Then, we selected the maceration extract to continue for fractions, with the ethyl acetate and n-butanol presenting the best results with antioxidant and anti-advanced glycation end products (AGEs) activity potential. Finally, the chemical composition was determinate by high-resolution mass spectrometry (HRMS) in combination with Global Natural Products Service (GNPS) data bank. As a result, this study indicated the presence of 29 phenolics compounds with reported activity as antioxidant and with potential of protein glycation, supported the diabetes activity of this plants. The n-butanolic fraction of R. glazioui showed compounds such as apigenin, kaempferol, quercetin, myricetin and chlorogenic acid, which have generated a half maximal inhibitory concentration (IC50) of 36.5 µg mL 1 for oxidative glycation inhibition, being highlighted as the most active. medicine Recently 3 world control line use here Thus blanchetii regnellii bioactivity collected aqueous maceration, , maceration) 2,2diphenyl1picrylhydrazyl 22diphenyl1picrylhydrazyl diphenylpicrylhydrazyl 2,2 diphenyl picrylhydrazyl 2 power Then fractions nbutanol n butanol antiadvanced anti advanced AGEs (AGEs Finally highresolution high resolution HRMS (HRMS GNPS (GNPS bank result plants nbutanolic butanolic R apigenin kaempferol quercetin acid IC50 IC (IC50 365 36 5 36. inhibition active 2diphenyl1picrylhydrazyl 22 2, IC5 (IC5 (IC

Resumo Introdução O conhecimento dos fatores relacionados à perda dentária contribui para o planejamento das ações preventivas realizadas pelas Equipes de Saúde Bucal (ESBs). Objetivo Avaliar o indicador de proporção de exodontia nos municípios do estado de São Paulo e a sua relação com os indicadores socioeconômicos e cobertura das ESBs. Método Estudo ecológico com dados secundários dos 645 municípios do estado de São Paulo do ano de 2012. A variável dependente foi o percentual de exodontia mensurado pelo indicador de proporção de exodontia em relação aos procedimentos individuais realizados pelas ESBs, disponibilizado pelo Sistema de Informação da Atenção Básica. Foram considerados como variáveis independentes a cobertura populacional estimada pelas ESBs, o Produto Interno Bruto (PIB) dos municípios per capita, o Índice de Desenvolvimento Humano (IDH) e o Índice Paulista de Responsabilidade Social (IPRS). Foram aplicados modelos lineares generalizados, avaliados pelo p-valor do teste de Wald, AICC e grau de liberdade. Resultados Apresentaram maior proporção de extrações dentárias as cidades do estado de São Paulo com menor cobertura de ESBs (p < 0,0001), com menor valor de IDH (p < 0,0001) e com maior valor de IPRS (p = 0,0018). Conclusão A baixa cobertura de ESBs e as desigualdades socioeconômicas contribuem para aumentar as extrações dentárias no estado de São Paulo.

Abstract Background Knowledge about factors associated with tooth loss contributes to the planning of preventive actions carried out by Oral Health Teams (OHT). Objective To evaluate the proportion of dental extraction indicator in the cities of the state of São Paulo, Brazil, and its relationship with socioeconomic indicators and coverage of the OHT. Method Ecological study using secondary data from 654 municipalities in the state of São Paulo conducted in 2012. The dependent variable was the percentage of dental extraction measured by the proportion of this indicator in relation to individual procedures performed by the OHT provided by the Primary Health Care (PHC) Information System. The population coverage by the OHT, the per capita Gross Domestic Product (GDP), the Human Development Index (HDI), and the São Paulo Social Responsibility Index (IPRS) of the municipalities were evaluated as independent variables. Generalized linear models, formulated by the Wald test p-value, Corrected Akaike Information Criterion (AICc), and degree of freedom, were applied. Results Higher proportions of dental extraction were found in the municipalities with lower coverage by OHT (p<0.0001), lower HDI value (p<0.0001), and higher IPRS value (p=0. 0018). Conclusion Low coverage by OHT and socioeconomic inequalities contribute to increased proportion of dental extractions in the state of São Paulo.

Abstract Breast cancer is one of the leading types of cancer worldwide, and the search for new treatment options are crucial. Nonsteroidal anti-inflammatory drugs (NSAIDs) -specially ibuprofen and diclofenac-, have shown antitumoral effect against several types of cancer. The synthesis of organometallic compounds has shown significant improvements in pharmacological properties and efficacy of organic molecules. Two zinc II ternary complexes containing the NSAIDs diclofenac and ibuprofen and nicotinamide neutral linker (Nic) were obtained by the two-step solvent metalligand complexation method. The compounds Zn2(Diclof)4(Nic)2 (complex 1) and Zn2(Ibup)4(Nic)2 (complex 2) were tested in breast cancer cell lines (4T1, MCF-7 and MDA-MB-231) to evaluate their cytotoxicity, comparing to ibuprofen and diclofenac as controls. We found that both complex 1 and 2 exerted more than 60% reduction in 4T1 viability at 250µM, and complex 2 decreased cell viability at 250 µM and 137.5 µM in MCF-7 (34.35% and 26.42% reduction, respectively) and in MDA-MB-231 (57.2% and 22.88% reduction, respectively), all compared to controls. Complex 1 was selective only in MCF-7, and complex 2 was selective in both MCF-7 and MDA-MB-231. In summary, our data showed that the cytotoxic effect of complex 1 and 2 is increased comparing to their original NSAID in different breast cancer cell lines, highlighting their potential anti-tumoral activity.