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Pancreatic cystic neoplasms (PCN) are frequently detected on abdominal images performed for non-pancreatic indications. Their prevalence in asymptomatic population ranges from 2.7 to 24.8%, and increases with age. There are several types of pancreatic cysts. Some may contain cancer or have malignant potential, such as mucinous cystic neoplasms, including mucinous cystadenoma (MCN) and intraductal papillary mucinous neoplasms (IPMN). In contrast, others are benign, such as serous cystadenoma (SCA). However, even those cysts with malignant potential rarely progress to cancer. Currently, the only treatment for pancreatic cysts is surgery, which is associated with high morbidity and occasional mortality. The Board of the Chilean Pancreas Club of the Chilean Gastroenterology Society developed the first Chilean multidisciplinary consensus for diagnosis, management, and surveillance of PCN. Thirty experts were invited and answered 21 statements with five possible alternatives: 1) fully agree; 2) partially agree; 3) undecided; 4) disagree and 5) strongly disagree. A consensus was adopted when at least 80% of the sum of the answers “fully agree” and “partially agree” was reached. The consensus was approved by the Board of Directors of the Chilean Pancreas Club for publication.

https://doi.org/10.4067/s0034-98872021001201773 73 downloads

Functional and genomic characterization of three novel cell lines derived from a metastatic gallbladder cancer tumor
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García, Patricia

; Bizama, Carolina

; Rosa, Lorena

; Espinoza, Jaime A.

; Weber, Helga

; Cerda-Infante, Javier

; Sánchez, Marianela

; Montecinos, Viviana P.

; Lorenzo-Bermejo, Justo

; Boekstegers, Felix

; Dávila-López, Marcela

; Alfaro, Francisca

; Leiva-Acevedo, Claudia

; Parra, Zasha

; Romero, Diego

; Kato, Sumie

; Leal, Pamela

; Lagos, Marcela

; Roa, Juan Carlos

Biological Research

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2020, Volume 53 elocation 13

Resumo: En | Texto: En | PDF: En

Abstract Background: Gallbladder cancer (GBC) is the most common tumor of the biliary tract. The incidence of GBC shows a large geographic variability, being particularly frequent in Native American populations. In Chile, GBC represents the second cause of cancer-related death among women. We describe here the establishment of three novel cell lines derived from the ascitic fluid of a Chilean GBC patient, who presented 46% European, 36% Mapuche, 12% Aymara and 6% African ancestry. Results: After immunocytochemical staining of the primary cell culture, we isolated and comprehensively characterized three independent clones (PUC-GBC1, PUC-GBC2 and PUC-GBC3) by short tandem repeat DNA profiling and RNA sequencing as well as karyotype, doubling time, chemosensitivity, in vitro migration capability and in vivo tumorigenicity assay. Primary culture cells showed high expression of CK7, CK19, CA 19-9, MUC1 and MUC16, and negative expression of mesothelial markers. The three isolated clones displayed an epithelial phenotype and an abnormal structure and number of chromosomes. RNA sequencing confirmed the increased expression of cytokeratin and mucin genes, and also of TP53 and ERBB2 with some differences among the three cells lines, and revealed a novel exonic mutation in NF1. The PUC-GBC3 clone was the most aggressive according to histopathological features and the tumorigenic capacity in NSG mice. Conclusions: The first cell lines established from a Chilean GBC patient represent a new model for studying GBC in patients of Native American descent.

https://doi.org/10.1186/s40659-020-00282-7 1047 downloads

Functional and transcriptomic characterization of carboplatin-resistant A2780 ovarian cancer cell lines
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Viscarra, Tamara

; Buchegger, Kurt

; Jofre, Ignacio

; Riquelme, Ismael

; Zanella, Louise

; Abanto, Michel

; Parker, Alyssa C.

; Piccolo, Stephen R.

; Roa, Juan Carlos

; Ili, Carmen

; Brebi, Priscilla

Biological Research

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2019, Volume 52 elocation 13

Resumo: En | Texto: En | PDF: En

Abstract Background: Ovarian cancer is a significant cancer-related cause of death in women worldwide. The most used chemotherapeutic regimen is based on carboplatin (CBDCA). However, CBDCA resistance is the main obstacle to a better prognosis. An in vitro drug-resistant cell model would help in the understanding of molecular mechanisms underlying this drug-resistance phenomenon. The aim of this study was to characterize cellular and molecular changes of induced CBDCA-resistant ovarian cancer cell line A2780. Methods: The cell selection strategy used in this study was a dose-per-pulse method using a concentration of 100 μM for 2 h. Once 20 cycles of exposure to the drug were completed, the cell cultures showed a resistant phenotype. Then, the ovarian cancer cell line A2780 was grown with 100 μM of CBDCA (CBDCA-resistant cells) or without CBDCA (parental cells). After, a drug sensitivity assay, morphological analyses, cell death assays and a RNA-seq analysis were performed in CBDCA-resistant A2780 cells. Results: Microscopy on both parental and CBDCA-resistant A2780 cells showed similar characteristics in morphology and F-actin distribution within cells. In cell-death assays, parental A2780 cells showed a significant increase in phosphatidylserine translocation and caspase-3/7 cleavage compared to CBDCA-resistant A2780 cells (P < 0.05 and P < 0.005, respectively). Cell viability in parental A2780 cells was significantly decreased compared to CBDCA-resistant A2780 cells (P < 0.0005). The RNA-seq analysis showed 156 differentially expressed genes (DEGs) associated mainly to molecular functions. Conclusion: CBDCA-resistant A2780 ovarian cancer cells is a reliable model of CBDCA resistance that shows several DEGs involved in molecular functions such as transmembrane activity, protein binding to cell surface receptor and catalytic activity. Also, we found that the Wnt/3-catenin and integrin signaling pathway are the main metabolic pathway dysregulated in CBDCA-resistant A2780 cells.

https://doi.org/10.1186/s40659-019-0220-0 482 downloads

Recomendaciones sobre el manejo del cáncer de páncreas tipo adenocarcinoma en Latinoamérica: Reunión del Consenso del Simposio Latinoamericano de Gastroenterología Oncológica (SLAGO) y de la Asociación Ibero Latinoamericana de Terapia Radiante (ALATRO), Viña del Mar, Chile 2015
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Caglevic, Christian

; de la Torre, Marcela

; Mahave, Mauricio

; Müller, Bettina

; Solé, Sebastián

; Moscoso, Yuri

; De La Fuente, Hernán

; Roa, Juan Carlos

; Hoefler, Sebastián

; Butte, Jean M

; González M, Pablo

; O'Connor, Juan Manuel

; Torres, Javiera

; Pérez Encalada, Verónica

; Alarcón Cano, Daniel

; Ubillos, Luis

; Rolfo, Christian

; Lingua, Alejo

; Díaz Romero, Consuelo

; Padilla Rosciano, Alejandro

; Cuartero, Viviana

; Calderillo Ruiz, Germán

; Schwartsmann, Gilberto

; Kon Jara, Xavier

; Andrade G, Andrés

; Mas López, Luis

; Barajas, Olga

; Carballido, Marcela

; Lembach, Hanns

; Morillas G, Lena

; Roca, Enrique

; Lobatón, José

; Montenegro B, Paola

; Yepes, Andrés

; Marsiglia, Hugo

Revista médica de Chile

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Out 2016, Volume 144 Nº 10 Páginas 1305 - 1318

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Pancreatic cancer is a malignancy of great impact in developed countries and is having an increasing impact in Latin America. Incidence and mortality rates are similar for this cancer. This is an important reason to offer to the patients the best treatments available. During the Latin American Symposium of Gastroenterology Oncology (SLAGO) held in Viña del Mar, Chile, in April 2015, a multidisciplinary group of specialists in the field met to discuss about this disease. The main conclusions of this meeting, where practitioners from most of Latin American countries participated, are listed in this consensus that seek to serve as a guide for better decision making for patients with pancreatic cancer in Latin America.

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Reprimo as a modulator of cell migration and invasion in the MDA-MB-231 breast cancer cell line
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Buchegger, Kurt

; Ili, Carmen

; Riquelme, Ismael

; Letelier, Pablo

; Corvalán, Alejandro H.

; Brebi, Priscilla

; Huang, Tim Hui-Ming

; Roa, Juan Carlos

Biological Research

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2016, Volume 49 Páginas 1 - 10

Resumo: En | Texto: En | PDF: En

BACKGROUND: Reprimo (RPRM), a highly glycosylated protein, is a new downstream effector of p53-induced cell cycle arrest at the G2/M checkpoint, and a putative tumor suppressor gene frequently silenced via methylation of its promoter region in several malignances. The aim of this study was to characterize the epigenetic inactivation and its biological function in BC cell lines. METHODS: The correlation between RPRM methylation and loss of mRNA expression was assessed in six breast cancer cell lines by methylation specific PCR (MSP), 5'-Aza-2'-deoxycytidine treatment and RT-PCR assays. MDA-MB-231 cells were chosen to investigate the phenotypic effect of RPRM in cell proliferation, cell cycle, cell death, cell migration and invasion. RESULTS: In the cancer methylome system (CMS) (web-based system for visualizing and analyzing genome-wide methylation data of human cancers), the CpG island region of RPRM (1.1 kb) was hypermethylated in breast cancer compared to normal breast tissue; more interesting still was that ERa(+) tumors showed higher methylation intensity than ERa(-). Downregulation of RPRM mRNA by methylation was confirmed in MDA-MB-231 and BT-20 cell lines. In addition, overexpression of RPRM in MDA-MB-231 cells resulted in decreased rates of cell migration, wound healing and invasion in vitro. However, RPRM overexpression did not alter cell viability, phosphatidylserine (PS) translocation or G2/M cell cycle transition. CONCLUSION: Taken together, these data suggest that RPRM is involved in decreased cell migration and invasion in vitro, acting as a potential tumor suppressor gene in the MDA-MB-231 cell line.

https://doi.org/10.1186/s40659-016-0066-7 1430 downloads

Disección submucosa endoscópica en cáncer gástrico incipiente: experiencia inicial en el Hospital Clínico de la Pontificia Universidad Católica de Chile
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Donoso D, Andrés

; Sharp, Allan

; Parra-Blanco, Adolfo

; Roa, Juan Carlos

; Bächler, Jean Phillipe

; Crovari, Fernando

; Funke, Ricardo

; Pimentel, Fernando

; Ibáñez, Luis

; Guzmán, Sergio

Revista médica de Chile

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Out 2015, Volume 143 Nº 10 Páginas 1277 - 1285

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Background: Endoscopic submucosal dissection (ESD) is a minimally invasive procedure that allows curative treatment of early gastric cancer (EGC) in selected patients. Aim: To report our initial experience with ESD. Material and Methods: Analysis of prospective data from 16 patients aged 61 to 84 years, who underwent ESD between December 2011 and June 2014. Tumor type, operative time, hospitalization length, oncologic outcomes, complications and short-term follow up were registered. Results: En-block resection was achieved in all cases. The median operative time was 135 min (range: 50-320 min). Specimens' median size was 3.5 cm (range: 3-10). All the resections were R0. In 14 patients ESD was considered curative. In two patients, ESD was considered potentially non-curative due to the presence pathological risk factors for lymph-node metastases in the biopsy specimen. Both patients underwent laparoscopic gastrectomy with lymph-node dissection. There was one case of gastric wall perforation that was repaired by laparoscopic suture. There was no mortality. The median follow-up time was 15 months (range: 2-30 months). Conclusions: ESD is a feasible and safe procedure in our institution with good results in this initial experience.

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Los Pericitos: Nuevos Enfoques en la Terapia Regenerativa, Patología Cerebrovascular y Cáncer
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Montiel-Eulefi, Enrique

; Barrientos Díaz, Leticia

; Leal, Pamela

; Roa, Juan Carlos

; Risopatrón, Jennie

; Salazar, Luis A

; Romero, Fernando

; Sánchez, Raúl

International Journal of Morphology

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Set 2011, Volume 29 Nº 3 Páginas 769 - 781

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Mesenchymal stem cells are a multipotent population of cells that have extensive capacity for differentiation, plasticity and immunosuppressive potential, making them an important tool in cell-based therapies. According to their potential, it has been determined that perivascular cells have stem cell characteristics of great clinical potential, however, the biological properties that lead to their differentiation are less understood. Recent advances in understanding the relationship between pericytes and mesenchymal stem cells pose tissue-specific functions, as well as their potential therapeutic use in ischemia, such as cerebro-vascular and a better understanding of the pathological tumor vascularization. Despite the growing acceptance that perivascular cells are related, or that they are mesenchymal stem cells, there is little experimental evidence to show the differentiation of pericytes in different cell types (Feng et al., 2010). In this review we document the biological basis of pericytes that support their use in regenerative therapy.

Las células madre mesenquimales son poblaciones multipotentes de células que tienen amplia capacidad de diferenciación, plasticidad y potencial inmunosupresor, lo que las hace una herramienta de gran importancia en las terapias basadas en células.En función de su potencial, se ha determinado que las células perivasculares poseen características de células madre de gran potencial clínico, no obstante, las propiedades biológicas que llevan a su diferenciación son menos comprendidas. Los últimos avances en la comprensión de la relación entre pericitos y las células madre mesenquimales plantean funciones específicas de tejido, así como, su potencial uso terapéutico en las isquemia, tales como, la cerebro-vascular y un mejor entendimiento de la vascularización patológica tumoral. A pesar de la creciente aceptación que las células perivasculares están relacionada o son células madre mesenquimales, existen escasas pruebas experimentales que muestren la diferenciación de pericitos en diferentes tipos de células (Feng et al., 2010). En esta revisión documentamos los fundamentos biológicos de los pericitos que respaldan su uso en terapia regenerativa.

28413 downloads

Survival of a cohort of patients with Intermediate and advanced gall bladder cancer treated with a prospective therapeutic protocol
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Manterola, Carlos

; Vial, Manuel

; Roa, Juan Carlos

Acta Cirúrgica Brasileira

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Jun 2010, Volume 25 Nº 3 Páginas 225 - 230

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

OBJETIVO: Avaliar os resultados de resultados da aplicação de um protocolo terapêutico de natureza prospectiva, com seguimento em longo prazo nos termos de taxas de sobrevivência em uma coorte de pacientes operados com carcinoma vesícula biliar (CVB) intermédio e avançado. MÉTODOS: A coorte prospectiva de pacientes com estágios intermediários e avançados de CVB tratados entre 1996 e 2006. Todos os casos foram tratados com uma segmentectomia hepática parcial em segmentos IVb e V e uma dissecção linfonodal regional e seis ciclos de quimioterapia de ambulatório (5-FU e leukovorin). Com um tempo de seguimento médio de 31,5 meses, a morbidade, mortalidade operatória, hepático e infiltração linfática e atuarial de sobrevida foram medidas. Estatísticas descritivas foram aplicadas, bem como análise bivariada aplicando o teste exato de Fisher, testes não-paramétricos, curvas de sobrevida Kaplan Meier e técnica de regressão logística e risco proporcional de Cox. RESULTADOS: Foram incluídos 40 pacientes neste protocolo, com uma média de idade de 59,5 anos (40-85 anos), dos quais 28 eram mulheres (70%). Profundidade de infiltração parede: muscular 8 pacientes (20%), subserosal 12 pacientes (30%), serosas 12 pacientes (30%) e perivesicular no tecido adiposo, 8 pacientes (20%). A série morbidade foi de 27,5%. Não houve mortalidade operatória. A quimioterapia foi bem tolerada. A sobrevida global atuarial da série foi de 50% em 60 meses. CONCLUSÃO: Nosso protocolo tem tratamento morbidade, mortalidade e taxas de sobrevivência semelhantes às relatadas anteriormente série.

PURPOSE: To evaluate the results of a prospective therapeutic protocol with long-term follow up in terms of survival rates in a cohort of patients treated with Intermediate and Advanced GBC (GBC). METHODS: Prospective cohort of patients with intermediate and advanced stages of GBC treated between 1996 and 2006. All cases were treated with a partial hepatic segmentectomy on segments IVb and V and a regional lymph node dissection and six cycles of out-patient chemotherapy (5-FU and leukovorin). With an average follow-up of 31.5 months, the morbidity, operative mortality, hepatic and lymphatic infiltration and actuarial survival were measured. Descriptive statistics were applied as well as bivariate analysis applying Fisher's exact test and non-parametrical tests and Kaplan Meier survival curves. Also logistic regression and proportional risk of Cox were applied. RESULTS: 40 patients were included in this protocol, with an average age of 59.5 years (40-85 years), of which 28 were women (70%). Depth of wall infiltration: muscular 8 patients (20%), subserosal 12 patients (30%), serosal 12 patients (30%) and perivesicular adipose tissue 8 patients (20%). The series morbidity was 27.5%. There was no operative mortality. The chemotherapy was well tolerated. The overall actuarial survival in the series was 50% at 60 months. CONCLUSION: Our protocol treatment has morbidity, mortality and survival rates similar to previously reported series.

2314 downloads Citado 2 vezes em SciELO

Efectos de la reforma laboral: ¿más trabajo y menos empleos?
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Guataquí Roa, Juan Carlos

; García Suaza, Andrés Felipe

Ensayos sobre POLÍTICA ECONÓMICA

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Dez 2009, Volume 27 Nº 60 Páginas 46 - 79

Resumo: Es En Pt | Texto: Es En Pt | PDF: Es | PDF: En | PDF: Pt

La reforma laboral de 2002 apuntó a dos objetivos específicos: la implementación de mecanismos de protección social y la flexibilización laboral. En este documento se analizan algunos de sus efectos, haciendo énfasis en la variación de las modalidades de contratación, los cambios en las horas de trabajo demandadas y los efectos en la duración del desempleo. De este análisis se concluye que la reforma tuvo un efecto intensivo en generar mayor demanda de horas de trabajo, pero que su efecto extensivo de generación de más empleos sólo fue marginal. Por otra parte, la duración del desempleo se redujo en el agregado. Clasificación JEL: J23, J64, J68.

The Colombian 2002 labor reform was aimed at two specific purposes: implementation of social protection measures and labor law flexibilization. On this document we analyze some of the effects of the reform, with specific emphasis on the potential changes on labor contract modalities, working hours and unemployment duration. From our analysis we conclude that the reform had an intensive effect (higher demand of working hours) yet it only had a marginal extensive effect over employment. It reduced unemployment duration as well. JEL classification: J23, J64, J68.

A Reforma Trabalhista de 2002 apontou a dois objetivos específicos: implementação de mecanismos de proteção social e flexibilização trabalhista. Neste documento se analisam alguns dos seus efeitos fazendo ênfase na variação das modalidades de contratação, nas mudanças nas horas de trabalho demandadas e os efeitos na duração do desemprego. Desta análise se conclui que a Reforma teve um efeito intensivo em gerar maior demanda de horas de trabalho mas que o seu efeito extensivo de geração de mais empregos só foi marginal. Por outro lado, a duração do desemprego reduziu-se no agregado. Classificação JEL: J23, J64, J68.

2381 downloads Citado 1 vez em SciELO

10.

Caracterización instrumental del desplazamiento forzado en Colombia: hechos estilizados y la Encuesta Continua de Hogares (2001-2006)
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Silva Arias, Adriana Carolina

; Guataquí Roa, Juan Carlos

Revista de Ciencias Sociales

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Dez 2008, Volume 14 Nº 3 Páginas 439 - 452

Resumo: Es En | Texto: Es En | PDF: Es | PDF: En

Como una alternativa a la problemática que gira en torno a la dimensión real del desplazamiento forzado en Colombia, este artículo analiza el poder explicativo de la caracterización instrumental del desplazamiento forzado a partir de la Encuesta Continua de Hogares (ECH) como un sistema alternativo de información. Este estudio gira en torno a la capacidad que tendría la ECH de replicar, a nivel de microdatos, algunos patrones recurrentes detectados en múltiples estudios a comunidades desplazadas. Para esto, evaluamos algunos elementos particulares de la población desplazada, tales como su nivel educativo, patrones de género, tamaño y tasas de dependencia de las familias desplazadas, así como patrones de inserción laboral. En general, la caracterización del desplazamiento forzado a través de la ECH para el periodo 2001-2006, refleja patrones identificados en la literatura sobre el desplazamiento en Colombia; presenta perfiles empíricos similares a los encontrados en estudios muestrales y en sistemas de información sobre desplazamiento forzado. Adicionalmente, este sistema de información exhibe comportamientos diferenciales entre los migrantes económicos voluntarios e individuos desplazados (IDPs - Internally Displaced Individuals) acorde con el análisis teórico de selección del migrante postulado por Borjas (1982). De esta manera, se evidenció entre los desplazados una alta proporción de menores de edad, así como una alta tasa de dependencia económica; además una dimensión de género ligada a la descomposición familiar, debido a que se encontró mayor proporción de hogares desplazados con jefatura femenina, así como mayor proporción de viudas, siendo las mujeres las que devengaron menores ingresos. El tratamiento empírico ofrece dos conclusiones destacables. La primera de ellas es reforzar la existencia de ciertos hechos estilizados en el perfil de los desplazados forzados en Colombia. La segunda es que la caracterización del desplazamiento a partir de la migración involuntaria detectada en la ECH refuerza los hechos estilizados y permite el desarrollo de ejercicios analíticos mucho más elaborados.

As an alternative to the problem revolving around the real dimension of forced displacement in Colombia, this paper analyze the explanatory power of instrumental characterization of forced displacement from the Continuous Household Survey (ECH) as an alternative information system. This study focuses on the capacity that ECH would have to replicate on the microdata level, some of the recurrent patterns detected in multiple studies of displaced communities. Some particular elements of the displaced population were evaluated, such as their level of education, patterns of gender, size and dependency rates of displaced households, as well as labor insertion patterns. In general, the characterization of forced displacement through the ECH for 2001-2006 reflected patterns identified in the literature on displacement in Colombia; it presented empirical profiles similar to those found in sample studies and information systems about forced displacement. Additionally, this information system displays differential behaviors between voluntary economic migrants and displaced individuals (IDPs-Internally Displaced Persons) consistent with the theoretical analysis of migrant selection postulated by Borjas (1982). Among the displaced population, a greater proportion of minors as well as a high economic dependency rate were shown; moreover, a gender dimension linked to family decomposition was found, because a higher proportion of displaced households were headed by women, as well as a higher proportion of widows, and women earned lower revenues. The empirical treatment offers two significant conclusions. The first reinforces the existence of certain facts stylized in the forced displacement profile in Colombia. The second is that the characterization of displacement from involuntary migration detected in the ECH reinforces stylized facts and enables the development of much more elaborate analytical exercises.

400 downloads

11.

Prevalencia de la infección genital por virus papiloma humano en hombres universitarios voluntarios de la IX Región, Chile
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Guzmán, Pablo

; Ili, Carmen

; Rifo, Patricio

; Briceño, Gastón

; Araya, Juan

; Villaseca, Miguel

; Roa, Juan Carlos

Revista médica de Chile

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Nov 2008, Volume 136 Nº 11 Páginas 1381 - 1389

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Background: Human papillomavirus (HPV) infection is the most common sexually transmitted disease. Aim: To determine prevalence of HPV genital infection in voluntary asymptomatic male university students. Material and methods: A cross-sectional study in 62 asymptomatic, sexually active male students. Exfoliated cells were obtained from the penüe shaft and coronal sulcus. Samples were analyzed for HPV DNA detection and genotyping by polymerase chain reaction and Reverse Line Blot. Results: The prevalence of HPV infection was 84%. HPV detection was 77% in penile shaft and 66% in coronal sulcus. The most commonly detected types were HPV-16 (45%), HPV-11 (19%), HPV-6 (10%) and HPV-18 (9%). Múltiple infection wasfoundin 54%. The most frequent combinations were VPH11/16 (18%) and VPH16/18 (5%). Conclusions: HPV infection is highly frequent in asymptomatic male university students, high rísk HPV types were greatly predominant.

19033 downloads Citado 4 vezes em SciELO

12.

Hepatocarcinoma con componente sarcomatoide: Caso clínico
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Losada M, Héctor

; Roa, Juan Carlos

; García M, Darío

; Araya, Juan Carlos

; Burgos SJ, Luis

; Silva A, Jorge

Revista médica de Chile

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Jun 2007, Volume 135 Nº 6 Páginas 768 - 772

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Hepatocarcinoma with sarcomatoid transformation is uncommon. It presents clinically with ¡eucocytosis and fever, resembling a liver abscess. We report a 40 year-old male that presented pain in the right upper quadrant and fever. Abdominal imaging showed an hypodense image in the liver that resembled a liver abscess. The patient was subjected to a percutaneous drainage obtaining 150 ml of an hemorrhagic fluid whose culture was negative. The clinical picture persisted and the patient was subjected to a right hepatectomy. The pathological study of the surgical piece disclosed a hepatocarcinoma with sarcomatoid transformation. The patient was discharged sixteen days after surgery

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13.

Descripción de genotipos de Echinococcus granulosus obtenidos de especímenes de hidatidosis humana
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MANTEROLA, CARLOS

; MELO, ANGÉLICA

; VIAL, MANUEL

; ROA, JUAN CARLOS

; MORA, JAVIER

Revista chilena de cirugía

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Dez 2006, Volume 58 Nº 6 Páginas 441 - 446

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Introduction: Environmental variability of Echinococcus granulosus (Eg) has been described. 10 genotypes have been reported as well as a certain intergenotype heterogenicity in studies with material of animal origin. The aim of this study is to describe the results of an Eg genotypification protocol in samples of human hydatidosis. Materials and methods: Cross-sectional study. The hydatid fluid was collected from a consecutive sample of patients operated on for hepatic and pulmonary hydatidosis at hospitals in Temuco between July, 2004 and September, 2005. A DNA extraction protocol was designed for Eg in homogenized samples of hydatid cyst aspirate. 2 polymerase chain reaction (PCR) systems were used: PCREg9 and PCREg16; both in concentrations of 2mM MgCl2. For PCREg9, Eg9F and Eg9R primers were used at concentrations of 0.5 mM, using 35 cycles with a hybridization temperature of 60°C. For PCREg16 Eg16F and Eg16R primers were used at concentrations of 0.5 mM, using 35 cycles with a hybridization temperature of 65°C. The PCR products were absorbed with a restriction enzyme (Rsa1) in order to distinguish the genotypes. Results: 25 samples were analyzed, 4 from pulmonary cysts and 21 from hepatic cysts. Eg amplification was achieved in 22 of the 25 samples (88%). The enzymatic digestion revealed the presence of 3 possible genotypes: in 21 of 22 samples (95.45%) a restriction pattern was observed corresponding to the G1 or G7 genotypes, and the remaining samples to the G4 or G7 genotypes. Conclusion: With the PCR systems used, Eg DNA was detected

Introducción: Se ha descrito variabilidad ambiental de Echinococcus granulosus (Eg). Se han reportado 10 genotipos y cierta heterogenicidad intergenotipo en estudios con material proveniente de animales. El objetivo de este estudio es describir los resultados de un protocolo de genotipificación de Eg en muestras de hidatidosis humana. Material y método: Estudio de corte transversal. Se recolectó el líquido hidatídico de una muestra consecutiva de pacientes intervenidos quirúrgicamente por hidatidosis hepática y pulmonar en hospitales de Temuco entre julio de 2004 y septiembre de 2005. Se diseñó un protocolo de extracción de ADN para Eg en muestras homogeneizadas de aspirado de quistes hidatídicos. Se emplearon 2 sistemas de reacción de polimerasa en cadena (PCR): PCREg9 y PCREg16; ambos en concentraciones de MgCl2 al 2mM. Para PCREg9, se utilizaron los primers Eg9F y Eg9R a concentraciones de 0.5 mM, empleándose 35 ciclos con temperatura de hibridación a 60°C. Para PCREg16 se utilizaron los primers Eg16F y Eg16R a concentraciones de 0,5 mM, empleándose 35 ciclos con temperatura de hibridación a 65°C. Los productos de las PCR fueron digeridos con una enzima de restricción (Rsa1) para la discriminación de los genotipos. Resultados: Se analizaron 25 muestras, 4 provenientes de quistes pulmonares y 21 de quistes hepáticos. Se logró amplificación de Eg en 22 de 25 muestras (88%). La digestión enzimática reveló la presencia de 3 genotipos posibles: en 21 de 22 muestras (95,45%) se observó un patrón de restricción correspondiente a los genotipos G1 ó G7 y en la muestra restante a los genotipos G4 ó G7. Conclusión: Con los sistemas de PCR empleados se detectó ADN de Eg

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TRANSCRIPTOS DE FUSIÓN DEL GEN BCR/ABL EN PACIENTES CON LEUCEMIA MIELOIDE CRÓNICA
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Artigas, Carmen Gloria

; Melo, Angélica

; Roa, Juan Carlos

; Roa, Iván

; Quijada, Ingrid

; Vittini, Cecilia

; Cabrera, María Elena

; Risueño, Concepción

International Journal of Morphology

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2003, Volume 21 Nº 3 Páginas 205 - 209

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

The most frequent cytogenetic aberration in the chronic myeloid leukemia (CML) is the Philadelphia chromosome, which is produced by the translocation t(9;22), molecular expression of BCR-ABL fusion gene, encodes a protein tyrosine kinase activity. Two different fusion proteins are produced depending on the breakpoint 210-kD (p210) and 190-kD (p190). The detection of this gene in the CML has diagnostic importance. Our goal was to detect fusion transcripts in patients with CML from the IX region of Chile. We studied 14 samples of blood from 11 patients with CML. Two of these patients were treated with Gleevec. The BCR-ABL gene was detected by reverse transcriptase polymerase chain reaction (RT-PCR). We found p210 fusion transcripts in 9/14 samples and p210 and p190 co-expression in 5/14. In two patients with Gleevec treatment, disappeared p190 transcript and remained p210 transcript. Our results reaffirm the importance of BCR-ABL fusion transcripts detection for diagnostic and following CML treatment

La anormalidad citogenética más común en la leucemia mieloide crónica (LMC) es el cromosoma Philadelphia, producida por la t(9;22), cuya expresión molecular es el gen de fusión BCR-ABL, que codifica proteínas con actividad tirosinquinasa. Según el punto de ruptura de los genes BCR o ABL se produce una proteína de fusión de 210-kD(p210) o 190-kD(p190). La presencia de este gen de fusión en pacientes con LMC tiene implicancia diagnóstica. Con el propósito de detectar transcriptos de fusión del gen BCR/ABL en pacientes con leucemia mieloide crónica, procedentes de la IX Región de Chile, se estudiaron 14 muestras de sangre de 11 pacientes con LMC. A 2 de ellos, se les realizó seguimiento durante su tratamiento con Gleevec. Se aplicó la técnica de reacción en cadena de la polimerasa con transcriptasa reversa (RT-PCR), usando una PCR en nido. Para la detección de los transcriptos de fusión p210 y p190 del gen BCR/ABL, se utilizaron 4 pares de iniciadores. En 9/14 muestras se detectó el transcripto de fusión p210 y en 5/14 los transcriptos de fusión p210 y p190. En los 2 pacientes en seguimiento, hubo desaparición del transcripto p190, permaneciendo el transcripto p210. Estos resultados reafirman la importancia de detectar transcriptos de fusión BCR/ABL para el diagnóstico y seguimiento durante el tratamiento de la LMC

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