ABSTRACT Purpose: To characterize the extracellular vesicle protein cargo in the aqueous humor and plasma of patients with ocular toxoplasmosis. Methods: Aqueous humor and plasma were collected from six patients with active ocular toxoplasmosis and six patients with cataract. Extracellular vesicles were isolated, and western blotting and mass spectrometry were performed for protein analysis. Results: All plasma samples from patients with ocular toxoplasmosis and cataract were positive for the tetraspanins CD63 and TSG101. However, the aqueous humor from patients with ocular toxoplasmosis was positive only for CD63. Sixty-seven new unreported proteins were identified in the aqueous humor and plasma of patients with the ocular toxoplasmosis and cataract. Of the 67 proteins, 10 and 7 were found only in the cataract and ocular toxoplasmosis groups, respectively. In general, these proteins were involved in immune system activation and retina homeostasis and were related to infections and retina-associated diseases. Conclusion: The distinct protein signatures between ocular toxoplasmosis and cataract may be helpful in the differential diagnosis of ocular toxoplasmosis. However, more studies are needed to better understand the role of these proteins in the pathogenesis of ocular toxoplasmosis. Purpose Methods isolated analysis Results CD CD6 TSG101 TSG However Sixtyseven Sixty seven 6 1 groups respectively general retinaassociated associated diseases Conclusion TSG10 TSG1

ABSTRACT New management practices applied to coffee crops may influence the soil’s capacity to tolerate vertical stresses. This paper aimed to evaluate the influence of two coffee crop management systems on the soil load-bearing capacity and critical soil water content to agricultural machinery traffic. This study was performed in the experimental area of the Federal Institute of the Southeast of Minas Gerais - Rio Pomba college, in Rio Pomba city, Minas Gerais, Brazil. Dystrophic Red-Yellow Oxisol (Latossolo Vermelho-Amarelo distrófico) (LVA7) with clayed texture predominating in the experimental unit. Undisturbed soil samples were collected from layers of 0.00-0.03, 0.12-0.15 and 0.27-0.30 m, randomly, in the center of the interrows of coffee plants (Coffea arabica L.) in monoculture plots under traditional management (in full sun) and in the plots of coffee plants intercropped with gliricidia (Gliricidia sepium) (shaded) to estimate pre-consolidation pressures, through uniaxial compression tests and adjustment of soil load-bearing capacity models. The average and maximum normal stresses applied to the soil and the vertical stress distribution of three agricultural tractors used in mechanized farming operations were estimated, and the critical soil water content to the traffic of these tractors was determined for both treatments, aiding in the decision-making process regarding additional compaction risks in the area. Cultivation of gliricidia in consortium with coffee did not influence the soil load-bearing capacity. The soil layer of 0.12-0.15 m was the most vulnerable to vertical stresses in both treatments. Agricultural tractors Agrale 4100, MF 265 and MF 275 presented values of vertical stresses of 335.76, 200.24 and 245.55 kPa, respectively, and the soil water content for the traffic of agricultural machines without plastic deformation was higher in the coffee plants in full sun for all studied depths.

A estimativa da área foliar total (AFT) é uma importante característica das culturas cuja avaliação é um desafio visto que métodos diretos de obtê-la são destrutivos e consome tempo. Métodos não destrutivos têm sido explorados com vista a se obter a AFT por meio de abordagens indiretas e, por sua vez, a criação de outros recursos, como o índice de área foliar. O desenvolvimento de tecnologia não destrutiva para acessar a AFT de uma planta tem sido objeto de muitas pesquisas e a metrologia óptica é uma abordagem promissora. Neste trabalho alguns métodos indiretos associados com as abordagens ópticas foram avaliados como alternativa para obtenção da AFT do cafeeiro. Equipamentos comerciais foram usados, como uma câmera com lente olho de peixe e medidas de lux, associadas às dimensões das copas, testados e comparados com outros métodos não destrutivos e com métodos propostos na literatura. A associação entre produção e a AFT estimada também foi avaliada. Os resultados mostraram que os equipamentos comerciais, geralmente utilizados em silvicultura, não se coadunam como as melhores aproximações para cafeeiros enquanto métodos relacionados às dimensões e valores de lux das plantas foram as melhores alternativas para estimar a AFT do cafeeiro.

The total leaf area (TLA) estimation is an important feature of the crops and their assessment a challenge, since the direct methods of obtaining it are destructive and time consuming. Non-destructive methods have been explored to obtain the TLA by indirect approaches, in turn creating other features, as the leaf area index. The development of non-destructive technology to access the TLA of a plant has been the subject of much research, and the optical metrology is an promising approach. In this work, some indirect methods associated with optical approaches were evaluated as an alternative to obtain the TLA of the coffee plant. Commercial equipment were used, such as a camera with a fish eye lens and lux meters, associated to the sizes of the canopies were tested and compared to another non-destructive method and with methods proposed in the literature. The association between production and the TLA estimated was also evaluated. The results showed that the commercial equipment, generally used in forestry, was not the best approach in coffee plants, and that the methods related to the size and lux values of the plants were the best alternatives to estimate the TLA of the coffee plant.