ABSTRACT. The repercussions of Long COVID demand specialised rehabilitation care. Interdisciplinary interventions in a 58-year-old patient were implemented to improve the clinical, motor and cognitive complaints associated with COVID-19. The rehabilitation team performed quantitative and qualitative evaluations in the initial phase and after 12 months of follow-up. The patient’s neuropathic pain, ankle and foot muscle strength, gait pattern, general cognitive functioning, initiative, emotional expressiveness, processing speed, neuropsychiatric symptoms and quality of life improved. She demonstrated gains in metacognition and expanded the use of compensatory strategies, resuming her routine and professional activities, although still with signs of executive dysfunction. It is concluded that a rehabilitation program calibrated to the profile of the patient with Long COVID had positive effects on functionality and satisfaction with quality of life. ABSTRACT care 58yearold yearold 58 year old clinical COVID19. COVID19 19. 19 COVID-19 1 followup. followup follow up. up follow-up patients s pain strength pattern functioning initiative expressiveness speed improved strategies activities dysfunction 5 COVID1 COVID-1 COVID-

RESUMO. As repercussões da COVID Longa demandam atendimentos especializados de reabilitação. Intervenções interdisciplinares em paciente de 58 anos foram implementadas com o objetivo de melhorar as queixas clínicas, motoras e cognitivas associadas à COVID-19. A equipe de reabilitação realizou avaliações quantiqualitativas na fase inicial e após 12 meses de acompanhamento. A paciente evoluiu com melhora da dor neuropática, da força muscular de tornozelo e pé, do padrão de marcha, do funcionamento cognitivo geral, da iniciativa, da expressividade emocional, da velocidade de processamento, dos sintomas neuropsiquiátricos e da qualidade de vida (domínios físico e psicológico). Demonstrou ganhos na metacognição e ampliou o uso de estratégias compensatórias, retomando suas atividades rotineiras e profissionais, embora ainda com indícios de disfunção executiva. Conclui-se que um programa de reabilitação calibrado ao perfil do paciente com COVID longa trouxe efeitos positivos na funcionalidade e satisfação com a qualidade de vida. RESUMO 5 clínicas COVID19. COVID19 19. 19 COVID-19 1 acompanhamento neuropática pé marcha geral iniciativa emocional processamento domínios psicológico. psicológico . psicológico) compensatórias profissionais executiva Concluise Conclui se COVID1 COVID-1 COVID-

ABSTRACT This study discusses theoretical reverberations that stand out in the field of Physical Education (PE) in Brazil in the face of epistemological discussions demarcated within the scope of PE in the USA from the 1960s onwards, and due to the contributions of the Disciplinary Movement and the context by which Kinesiology then established its position as a scope in the field of American PE. An exploratory literature review was carried out with the following guiding question: what is the impact of the epistemological developments of North American PE on the configuration of the general theory of PE in Brazil? From this perspective, we verified the authors in the Brazilian field who took a position on the resonances and limits arising from the conjectures of the scientific field of PE in the USA. Through this investigation, some processes of inhibition were highlighted by which the proposal of Kinesiology could not increase reflections in Brazil, with few exceptions. (PE s onwards question perspective investigation exceptions

RESUMO Este estudo discute reverberações teóricas que se destacaram no campo da Educação Física (EF) no Brasil diante das investigações epistemológicas demarcadas no âmbito da EF nos EUA a partir da década de 1960, e devido às contribuições do Movimento Disciplinar e ao contexto pelo qual a Cinesiologia se estabeleceu então como escopo no campo da EF americana. Foi realizada uma revisão exploratória da literatura com a seguinte questão norteadora: qual impacto dos desenvolvimentos epistemológicos da EF norte-americana na configuração da teoria geral da EF no Brasil? Nessa perspectiva, verificamos os autores do campo brasileiro que adotaram uma posição sobre as ressonâncias e limites decorrentes das conjecturas do campo científico da EF nos EUA. Através desta investigação, foram destacados alguns processos de inibição pelos quais a proposta da Cinesiologia não conseguiu aumentar reflexões no Brasil, com poucas exceções. (EF 1960 americana norteadora norteamericana norte perspectiva investigação exceções 196 19 1

ABSTRACT BACKGROUND AND OBJECTIVES: In the teacher’s professional practice, there is a high level of stress, anxiety and incidence of pain. With the advent of COVID-19 and the emergence of remote teaching, it is possible that this scenario has been aggravated. The objective of this study was to evaluate chronic pain, central sensitization and pain catastrophizing among primary education teachers during remote teaching offered due to the pandemic. METHODS: A cross-sectional analytical observational study. Participants were 200 teachers from different regions of Brazil who responded through an online form a sociodemographic questionnaire and a questionnaire about their working conditions. In addition, pain intensity was assessed using the Visual Analog Pain Scale (VAS), central sensitization state was assessed using the Central Sensitization Questionnaire (CSI), and pain catastrophizing was assessed using the Catastrophic Thinking Scale about Pain (PCS). RESULTS: Most of the teachers were female, white, from the southeast region, with lato sensu specialization, had an income of 2 to 2.5 minimum wages, working 21 to 40 hours a week. Most of the teachers reported pain intensity equal to eight and the body region most affected was the lumbar spine. It was observed that teachers with low salary, uncomfortable environment and longer days dedicated to remote teaching showed a greater tendency to central sensitization and pain catastrophizing. Most teachers reported pain intensity equal to eight and the most affected body region was the lumbar spine. Teachers with uncomfortable furniture reported increased pain, especially in the lumbar spine and neck. They also showed higher levels of central sensitization to pain, inversely proportional to their salary income and, in sum, teachers with a greater feeling of discomfort catastrophized more which reflects the physical and emotional damage that pain can cause. CONCLUSION: Remote teaching during the pandemic of COVID-19 impacted physical and emotional changes in teachers of primary education. The professionals perceived that their furniture was not the most adequate for the high amount of time they had to work on academic activities using computers (in general, over 40 hours a week), they reported increased pain, especially in the lumbar spine and neck, they presented higher levels of central pain sensitization, which was influenced by low salary income, and, in sum, teachers with higher feelings of discomfort catastrophized more, which reflects the physical and emotional damage that pain may cause. All these affections tend to reduce the teachers’ quality of life and, consequently, affect the teaching and learning processes. OBJECTIVES teacher s practice stress COVID19 COVID 19 COVID-1 aggravated METHODS crosssectional cross sectional 20 conditions addition VAS, VAS , (VAS) CSI, CSI (CSI) PCS. PCS . (PCS) RESULTS female white specialization 25 5 2. wages 4 week neck sum cause CONCLUSION general week, week) consequently processes COVID1 1 COVID- (VAS (CSI (PCS

RESUMO JUSTIFICATIVA E OBJETIVOS: Na atuação profissional do professor, há elevado nível de estresse, ansiedade e incidência de dor. Com o advento do COVID-19 e a emergência do ensino remoto, é possível que este cenário tenha sido agravado. O objetivo deste estudo foi avaliar quadros de dor crônica, sensibilização central e catastrofização da dor em professores da rede básica durante o ensino remoto ofertado devido à pandemia. MÉTODOS: Trata-se de um estudo observacional analítico do tipo transversal. Participaram 200 professores de diferentes regiões do Brasil que responderam, através de um formulário online, perguntas sobre aspectos sociodemográficos e suas condições de trabalho, além de serem avaliadas a intensidade de dor por meio da escala analógica visual de dor, o estado de sensibilização central por meio do Questionário de Sensibilização Central (CSI) e a catastrofização da dor por meio da Escala de Pensamento Catastrófico sobre a Dor. RESULTADOS: A maioria dos professores eram do sexo feminino, brancos, da região sudeste, com especialização lato sensu, apresentavam renda de 2 a 2,5 salários-mínimos e jornada de trabalho de 21 a 40 horas semanais. A maioria dos professores relatou intensidade de dor igual a oito e a região do corpo mais afetada foi a coluna lombar. Observou-se que professores com baixo salário, ambiente desconfortável e maior jornada dedicada ao ensino remoto apresentaram maior tendência à sensibilização central e à catastrofização da dor. Professores com mobiliário desconfortável relataram aumento da dor, em especial, na coluna lombar e no pescoço. Apresentaram também maiores níveis de sensibilização central à dor, inversamente proporcional à sua renda salarial e, de maneira somatória, professores com maior sensação de desconforto catastrofizaram mais o que reflete os prejuízos físicos e emocionais que a dor pode causar. CONCLUSÃO: O ensino remoto durante a pandemia do COVID-19 impactou alterações físicas e emocionais nos professores da rede básica de ensino. Os profissionais perceberam que seu mobiliário não era o mais adequado para a alta permanência de tempo que tiveram de trabalhar em atividades acadêmicas pelo computador (em geral, acima de 40 horas semanais), relataram aumento da dor, em especial na coluna lombar e no pescoço, apresentaram maiores níveis de sensibilização central à dor, que foi influenciado por baixa renda salarial e, de maneira somatória, professores com maior sensação de desconforto catastrofizaram mais, o que reflete os prejuízos físicos e emocionais que a dor pode causar. Todos estes acometimentos tendem a reduzir a qualidade de vida do professor e, consequentemente, afetar o processo de ensino e aprendizagem. OBJETIVOS estresse COVID19 COVID 19 COVID-1 agravado crônica MÉTODOS Tratase Trata se transversal 20 responderam online CSI (CSI Dor RESULTADOS feminino brancos sudeste sensu 25 5 2, saláriosmínimos salários mínimos 4 semanais Observouse Observou salário pescoço somatória causar CONCLUSÃO geral semanais, , semanais) consequentemente aprendizagem COVID1 1 COVID-

Resumo Este artigo sintetiza aspectos do processo de disciplinarização da educação física (EF) nos Estados Unidos, de modo a fornecer elementos para pensar as relações entre ciência e educação em parâmetros marcadamente reflexivos. Nesse percurso, procura-se restituir alguns dos elementos fundantes do movimento disciplinar da EF norte-americana, bem como identificar suas contribuições e limites para a estruturação da área como um campo científico. Em termos metodológicos, o estudo configurou-se como teórico-bibliográfico e de vertente exploratória. A partir desse delineamento, foram inicialmente mobilizados artigos e capítulos de livros da literatura nacional e internacional atinentes ao debate epistemológico da EF e, posteriormente, analisadas as informações com base no referencial teórico de Pierre Bourdieu. Como conclusão, destaca-se que o movimento disciplinar na EF norte-americana trouxe avanços e contribuições significativas para situar a EF na hierarquia da ciência e, ao mesmo tempo, justificar sua existência no ensino superior. Em que pesem, no entanto, esses aspectos producentes, o movimento disciplinar da EF não pôde conter os efeitos colaterais da dinâmica organizacional proposta, sobretudo no que tange à fragmentação da EF em diferentes subáreas que não apenas pouco se comunicam, mas também concorrem pela definição do todo no campo. De qualquer modo, o movimento disciplinar ao reconhecer no movimento humano o conector comum das diferentes frentes de investigação da área não só induziu como também tornou crível o processo de organização da EF como uma ciência autônoma.

Abstract This article summarizes aspects of the process of disciplining physical education (PE) in the United States, to provide elements for thinking about relationships between science and education in markedly reflexive parameters. In this way, we seek to restore some of the founding elements of the disciplinary movement of North American PE, as well as to identify its contributions and limits to the structuring of the area as a scientific field. In methodological terms, the study was configured as theoretical-bibliographic and exploratory. Based on this design, articles and book chapters from national and international literature concerning the epistemological debate on PE were initially mobilized and, later, the information was analyzed based on Pierre Bourdieu’s theoretical framework. In conclusion, it is emphasized that the disciplinary movement in North American PE has brought significant advances and contributions to place PE in the hierarchy of science and, at the same time, justify its existence in higher education. However, despite these productive aspects, the PE disciplinary movement could not contain the side effects of the proposed organizational dynamics, especially concerning the fragmentation of PE in different subareas that not only communicate little but also compete for the definition in the field. In any case, the disciplinary movement, recognizing in human movement the common connector of the different research fronts in the area, not only induced but made credible the process of organizing PE as an autonomous science.

RESUMO O presente estudo objetivou agrupar algumas perspectivas sobre a atividade epistemológica de Go Tani no contexto da Educação Física (EF) brasileira, estabelecendo suas contribuições e reflexões a partir da Cinesiologia para a estruturação da graduação e da pós-graduação em Educação Física no Brasil. Trata-se de um estudo bibliográfico e exploratório, o qual priorizou a produção de Tani nas mais variadas plataformas, bem como os materiais que circularam sobre a Cinesiologia na literatura nacional e internacional. Por fim, através da referida revisão teórica, foi possível identificar que o campo da EF é um cenário repleto de disputas e que mesmo com as transformações dos paradigmas que inferem nas alternativas legítimas no contexto científico, as relações que são estabelecidas entre as distintas escolas de pensamento da EF têm eclodido num processo de interincompreensão que contribui com a circularidade de perspectivas e a manutenção de crenças no campo.

ABSTRACT This study aimed to group some perspectives on Go Tani's epistemological activity in the context of Brazilian Physical Education (PE), establishing his contributions and reflections on Kinesiology for the structuring of undergraduate and graduate Physical Education in Brazil. This is a bibliographical and exploratory study, which prioritized Tani's production on the most varied platforms and the materials that circulated about Kinesiology in national and international literature. Finally, through the theoretical review, it was possible to identify that the PE field is a scenario full of disputes and that even with the transformations of paradigms that infer the legitimate alternatives in the scientific context, the relationships that are established between the different schools of PE thought have erupted in a process of mutual incomprehension that contributes to the circularity of perspectives and the maintenance of beliefs in the field.

ABSTRACT Purpose To reduce false-negative rates (FNR) in sentinel lymph node biopsy (SLNB) of clinically positive (cN+) axilla in patients undergoing neoadjuvant chemotherapy (NAC). The removal of three or more lymph nodes with dual-tracer mapping including a radioisotope was used. However, in the Brazilian Unified Health System, the radioisotope tracer is not feasible in some hospitals. We conducted a cross-sectional study to evaluate the detection rate of sentinel lymph node (SLN) in patients who converted from cN+ to ycN0 after NAC using blue dye as a single-agent mapping tracer. Methods During the period of March 2018 to September 2019, 34 patients who underwent NAC with cN+ who converted to ycN0 were enrolled in the study. The SLNB was performed using blue dye as a single-agent mapping followed by axillary lymph node dissection (ALND). Results The detection rate of sentinel lymph node was of 85.3%, being SLNB not possible for five patients (14.7%), due to fibrosis. The mean number of removed SLN was 2.5. Conclusions The use of blue dye as a single-agent mapping tracer demonstrated an acceptable detection rate of 85.3%. Although the FNR was possible to be determined, the small sample size might overestimate this rate. The removal of three or more lymph nodes with single-agent mapping tracer might be indicated for breast cancer patients who converted to ycN0 after NAC in the Brazilian health public services, in which radioisotope tracer is not suitable.

O equilíbrio sagital descreve o alinhamento ideal da coluna vertebral no plano sagital, sendo resultante da interação entre a coluna e os membros inferiores, por meio da bacia. O entendimento do equilíbrio sagital ganhou importância principalmente na última década, uma vez que o desequilíbrio sagital se correlaciona diretamente com incapacidade e dor. Doenças que alterem este equilíbrio podem causar mal alinhamento sagital e desencadear mecanismos compensatórios, os quais tentam restaurar a postura ereta. A literatura relata parâmetros radiográficos clinicamente relevantes, que possuem relação estabelecida com escores clínicos, para avaliar o alinhamento espinopélvico. Este artigo tem como objetivo fornecer uma ampla revisão da literatura sobre os parâmetros espinopélvicos mais relevantes na prática clínica, e descrever os mecanismos compensatórios da pelve e membros inferiores.

Sagittal balance describes the optimal alignment of the spine in the sagittal plane, resulting from the interaction between the spine and lower limbs, via the pelvis. Understanding sagittal balance has gained importance, especially in the last decade, because sagittal imbalance correlates directly with disability and pain. Diseases that alter that balance cause sagittal malalignment and may trigger compensatory mechanisms. Certain radiographic parameters have been shown to be clinically relevant and to correlate with clinical scores in the evaluation of spinopelvic alignment. This article aims to provide a comprehensive review of the literature on the spinopelvic parameters that are most relevant in clinical practice, as well as to describe compensatory mechanisms of the pelvis and lower limbs.

ABSTRACT Objective To assess the utility of bioimpedance (BIA) and skinfolds thickness (SF) in body fat percentage measuring (%BF) compared to the reference method dual-energy x-ray absorptiometry (DXA) in Brazilian reproductive age women, as well as to estimate of inter- and intra-observer precision for SF. Subjects and methods 170 women aged 18-37 years with BMI between 18 and 39.9 kg/m2 were selected for this cross-sectional study. Body density was evaluated through equations proposed by Jackson, Pollock and Ward (1980) (EqJPW) and Petroski (1995) (EqPET), and %BF was estimated by BIA, DXA and Siri’s formula (1961). The SF were measured by two separate observers: A and B (to determine inter-observer variability), who measured the folds at three times with 10-minute interval between them (to determine intra-observer variability – we used only observer A). Results The %BF by DXA was higher than those measured by SF and BIA (p<0.01, for all) of 90 volunteers. The Lin coefficient of agreement was considered satisfactory for %BF values obtained by EqJPW and BIA (0.55) and moderate (0.76) for sum of SF (ΣSF) values obtained by EqJPW and EqPET. No agreement was observed for the values obtained by SF (EqJPW and EqPET), BIA and DXA. Analysis of inter- and intra-observer of 59 volunteers showed that different measures of SF thickness met acceptability standards, as well as the % BF. Conclusion BIA and SF measurements may underestimate %BF compared with DXA. In addition, BIA and SF measurements are not interchangeable with DXA. However, our results suggest the equation proposed by Jackson, Pollock and Ward (three skinfolds) compared to BIA are interchangeable to quantify the %BF in Brazilian women in reproductive age. Furthermore, our results show acceptable accuracy for intra- and inter-observer skinfold measurements. Arch Endocrinol Metab. 2020;64(3):257-68

Abstract The objective of this study was to determine how to support actions to increase the resilience and adaptation of socio-ecological systems in the semiarid region of Bahia State, focusing on the Fundo de Pasto Communities (FPCs) of the Uauá municipality. Primary (semistructured interviews with decision-makers and community leaders) and secondary data sources were used. Five dimensions of analysis were selected to organize the primary data within a framework of adaptive capacity for climate change: territorial, socio-cultural, environmental, economic and politico-institutional. For approximately 200 years, FPCs have been communally occupying and using the Caatinga biome based on agrosilvopastoral systems. The results showed the communities’ resilience in dealing with climate stresses in both the territorial and socio-cultural dimensions. This includes processes of self-organization in associations and in networks, which facilitate the flow of knowledge and collective decision-making processes. The results also identified the environmental, economic and politico-institutional dimensions of the climate risks to FPCs, which could potentially limit their adaptive capacity. socioecological socio ecological State (FPCs municipality semistructured decisionmakers decision makers leaders used change sociocultural, sociocultural cultural, cultural environmental politicoinstitutional. politicoinstitutional politico institutional. institutional 20 years communities selforganization self organization networks decisionmaking making 2

Resumo A pesquisa realizada entre 2017 e 2018 buscou produzir conhecimentos que subsidiem ações voltadas à resiliência e à adaptação de sistemas socioecológicos na região semiárida do estado da Bahia - Semiárido Baiano, com foco nas Comunidades de Fundo de Pasto (CFP) localizadas no município de Uauá. Utilizaram-se dados secundários e primários (entrevistas semiestruturadas com tomadores de decisão e lideranças comunitárias). Para a sistematização dos dados primários no marco de capacidades adaptativas para mudanças climáticas foram selecionadas cinco dimensões de análise: territorial, sociocultural, ambiental, econômica e político-institucional. As CFP ocupam e usam o bioma Caatinga há cerca de 200 anos de forma comunal, com base em sistemas agrossilvopastoris. Os resultados indicaram o caráter resiliente das comunidades para lidar com os estresses climáticos nas dimensões territorial e sociocultural, a partir de processos de auto-organização em associações e na organização em redes, por meio das quais fluem conhecimentos e são tomadas decisões coletivas. Os resultados também indicaram os riscos climáticos aos quais as CFP estão expostas nas dimensões ambiental, econômica e político-institucional, com potencial para limitar capacidades adaptativas. 201 Baiano (CFP Uauá Utilizaramse Utilizaram se entrevistas comunitárias. comunitárias . comunitárias) análise sociocultural ambiental políticoinstitucional. políticoinstitucional político institucional. institucional político-institucional 20 comunal agrossilvopastoris autoorganização auto redes coletivas políticoinstitucional, institucional, 2

ABSTRACT Introduction: Nontyphoidal Salmonella serotypes are the main cause of human food-borne infection, including several hospitalization cases in the developing countries. Aim: To detect the main serotypes and to characterize the antibiotic resistance of human non-enteric and enteric nontyphoidal Salmonella from clinical isolates in Brazil. Methods: Salmonella serotypes were identified by microbiological and molecular methods. Susceptibility testing to antibiotics was performed by agar disk diffusion. Real-time PCRs were carried out for the detection of the genus Salmonella as well as serotypes Typhimurium and Enteritidis. Results: A total of 307 nontyphoidal Salmonella were isolated from 289 different patients in a reference laboratory (LACEN-RS) from Southern Brazil in a six-year period (2010–2015). There were 45 isolates from emerging cases and 244 from sporadic cases in hospitalized patients. Non-enteric isolates were detected in 42.6% of the patients from sources such as urine, blood and other clinical fluids. Serological and PCR-specific tests demonstrated that Typhimurium (48.4%) and Enteritidis (18.3%) were the most frequent serotypes. Typhimurium isolates were generally resistant to three or more antibiotic classes, while Enteritidis isolates to one or two classes. Typhimurium was the most frequent serotype in all samples (48.4%), mainly among the hospitalized patients (55.6%), and presented the highest rates of multidrug resistance (59.3% of the isolates of this serotype). Further, the prevalence of this serotype increased along the years of the study in comparison to other nontyphoidal Salmonella serotypes. Conclusion: Greater public health attention should be given to prevent salmonellosis in the community and in hospital settings to reduce the rates of Typhimurium strains with multidrug resistance.

Resumo Analisou-se o desempenho físico-mecânico de placas e pavers com incorporação de rejeitos da coleta seletiva do município de Blumenau, SC. Tais rejeitos são compostos de orgânicos, polímeros, papéis, vidro e têxteis, que foram reduzidos de tamanho (2,4 mm a 19,0 mm) e substituíram o agregado miúdo natural nos percentuais de 5%, 10%, 20% e 30%. Para o compósito no estado fresco verificou-se o índice de consistência, e no estado endurecido determinaram-se massa específica aparente, absorção de água e resistência à compressão. As placas foram submetidas ao ensaio de resistência à flexão e à análise de microscopia eletrônica de varredura. A massa específica aparente das placas variou de 0,8 a 1,6 g.cm-3, e dos pavers, de 1,78 a 1,84 g.cm-3. Para menores quantidades de rejeitos incorporados, os dois materiais apresentaram índices de absorção de água que satisfazem as exigências de normas correlatas. As placas apresentaram valores de resistência à compressão que variaram de 4,7 MPa a 14,6 MPa. Na flexão, os valores atingidos foram de 0,1 MPa a 1,8 MPa. Os pavers alcançaram valores de resistência à compressão entre 10,8 MPa e 18,7 MPa. Pavers com 5% de incorporação de agregados de rejeitos seriam passíveis de ser aplicáveis em calçadas e meios-fios desde que estudos futuros garantam sua durabilidade.

Abstract This project analysed the physical-mechanical performance of plates and pavers with the incorporation of waste originated from the sorting process in the city of Blumenau, SC, Brazil. The waste consisted of organic waste, polymers, paper, glass and textile materials which were reduced in size (2.4 mm to 19.0 mm) and then used to replace natural aggregate at different ratios: 5%, 10%, 20% and 30%. The consistence of the fresh mortar and the physical-mechanical indices, the apparent specific gravity, water absorption and compressive strength of the hardened mortar were determined. Scanning electron microscopy analysis and flexural strength tests were also conducted. The bulk density of the plates and pavers ranged from 0.8 to 1.6 g.cm-3 and 1.78 to 1.84 g.cm-3 respectively. In the case of low incorporation of waste, both materials presented water absorption results that meet the requirements of correlated standards. The plates presented compressive strength values ranging from 4.7 MPa to 14.6 MPa. Flexural strength results reached values between 0.1 MPa and 1,8 MPa. The pavers achieved compressive strength values between 10.8 MPa and 18.7 MPa. Pavers with 5% incorporation of waste aggregates could be used in sidewalks and curbs, as long as future studies are able to demonstrate their durability.

RESUMO O sorgo é uma cultura que se destaca como alternativa ao milho devido a menor exigência em fertilidade do solo e maior tolerância ao déficit hídrico. A falta de informações quanto ao comportamento qualitativo dificulta a recomendação de cultivares de sorgo de diferentes propósitos. Objetivou-se avaliar a composição bromatológica e a digestibilidade in vitro da silagem de cultivares de sorgo de diferentes propósitos, em duas épocas de cultivo. O delineamento experimental utilizado foi em blocos casualizados, com os tratamentos dispostos em esquema de parcela subdividida, com quatro repetições. Os tratamentos da parcela corresponderam a seis híbridos de sorgo de diferentes propósitos (BRS 308 e BRS 310, graníferos; BR 655 e BRS 610, forrageiros; BR 506 e CMSXS 647, sacarinos). Os tratamentos da subparcela corresponderam a duas épocas de cultivo (primeira safra e segunda safra). A cultivar de sorgo forrageiro BRS 655 apresentou maior teor de carboidratos não fibrosos e menor teor de fibra potencialmente digestível. As cultivares de sorgo sacarino obtiveram os maiores teores de carboidratos solúveis, os menores teores de fibra insolúvel em detergente neutro indigestível na segunda safra, e as maiores digestibilidades in vitro da matéria seca. As silagens de sorgo sacarino e de sorgo forrageiro BRS 655 apresentam maior valor nutritivo.

ABSTRACT Sorghum is a crop that stands out as an alternative to corn due to lower soil fertility demand and increased tolerance to drought. Lack of information about the qualitative behaviour of sorghum hinders the recommendation of different purpose sorghum cultivars. The goal was to evaluate the chemical composition and in vitro digestibility of different purpose sorghum cultivar silages, at two cropping seasons. The trial was conducted at the Plant Production Department, Federal Institute of Education, Science and Technology of Rondônia, Colorado do Oeste campus, and chemical analyses and in vitro incubation were performed at the Laboratory of Animal Nutrition, Federal University of Mato Grosso, Cuiabá campus. The experimental design was a randomized block with a split-plot arrangement and four replications. Plot treatments consisted of six different purpose sorghum cultivars (BRS 308 and BRS 310, grain sorghum; BR 655 and BRS 610, forage sorghum; and BRS 506 and CMSXS 647, sweet sorghum). Split-plot treatments consisted of two cropping periods (first crop and second crop). Forage sorghum cultivar BRS 655 demonstrated higher non-fiber carbohydrate content and lower potentially digestible fibre content than the other cultivars did. Sweet sorghum cultivars had higher levels of water soluble carbohydrates and non-protein nitrogen based on protein, lower indigestible neutral detergent fibre content at second crop, and higher in vitro dry matter digestibility than the other cultivars. The silages of sweet sorghum cultivars BRS 506 and CMSXS 647, and forage sorghum cultivar BRS 655 presented higher nutritional values.

ABSTRACT Stevia rebaudiana (Bertoni) Bertoni, Asteraceae, is used as a food additive because its leaves are a source of steviol glycosides. There are examples of tissue culture based on micropropagation and phytochemical production of S. rebaudiana leaves but there are few studies on adventitious root culture of S. rebaudiana. More than 90% of the plants used in industry are harvested indiscriminately. In order to overcome this situation, the development of methodologies that employ biotechnology, such as root culture, provides suitable alternatives for the sustainable use of plants. The aim of this study was to compare morpho-anatomical transverse sections of S. rebaudiana roots grown in vitro and in vivo. The in vitro system used to maintain root cultures consisted of a gyratory shaker under dark and light conditions and a roller bottle system. Transverse sections of S. rebaudiana roots grown in vitro were structurally and morphologically different when compared to the control plant; roots artificially maintained in culture media can have their development affected by the degree of media aeration, sugar concentration, and light. GC–MS and TLC confirmed that S. rebaudiana roots grown in vitro have the ability to produce metabolites, which can be similar to those produced by wild plants.

PURPOSE : To investigate allometric relationships among body mass (BM), muzzle-tail length (MTL), and tibia length (TL) in Wistar rats and establish their growth rate change parameters. METHODS : Eighteen male and 18 female Wistar rats were studied from the 3rd to the 21st week of age. BM, MTL, and TL were measured daily, and relative growth was compared using allometry. RESULTS : A positive correlation between BM and MTL (p<0.05) and BM and TL (p<0.05) was observed. Males and females showed comparable curves; however, females had turning points at a younger age. The allometric relationship between BM and MTL presented a regular increase until reaching a mass of 351 g (males) and 405 g (females). BM and TL showed an initial increase until 185 g (males) and 182 g (females), and then reached a plateau that finished at 412 g (males) and 334 g (females), to display another increase. CONCLUSIONS : The allometric relationship of body mass with animal length and tibia length was comparable for male and female rats, with female rats maturing earlier. Animal longitudinal growth occurred in a single stage. In contrast, tibia length depicted two stages of accelerated growth with an intermediate period of deceleration.

<p>A reversed-phase liquid chromatographic method was developed for the assay of synephrine in <italic>Citrus aurantium</italic> dry extract. The chromatographic separation used a C18 (250 × 4.6 mm, 5 µm) column and a mobile phase consisting of acetonitrile: 0.01% orthophosphoric acid (pH 3.0) at a 15:85 ratio, flow rate of 1 mL min^-1 and detection at 220 nm. The detector response was linear in the range 15 - 45 mg mL^-1. Specificity and stability, indicating suitability of the method, were confirmed using stress conditions (acid and basic hydrolysis, oxidation and photolysis). Intraday precision yielded RSD values of less than 1.77% and interday precision of 2.5%. The average recovery accuracy of the test was 102.03%. The method was validated according to international guidelines and can be used in routine quality control and stability studies of <italic>Citrus aurantium</italic> preparations. The preliminary stability study showed that synephrine is susceptible to hydrolysis, oxidation and photolysis.</p>