Abstract TVB-N is one of the important indexes of the freshness of chilled pork. To present the freshness more accurately and completely, a visualization technology of chemical composition was applied and optimized for pork freshness detection. Two hyperspectral cameras covering visible and near-infrared wavebands were used to detect the TVB-N content. GA, SPA, iPLSR, and siPLSR were employed to select characteristic wavebands from the spectra data extracted from the hyperspectral images, and PLSR models were established on full wavebands and characteristic wavebands. According to the models established on the spectral data, TVB-N distribution maps were obtained, and URV and ICV were employed to evaluate the performance of distribution maps. The results showed that, both PLSR models established on the characteristic wavebands and full wavebands achieved good accuracy, and that the characteristic wavebands cluster was in the spectral range of 600-750 nm, 1020-1120 nm, and 1450-1570 nm. To optimize the performance of distribution maps, prediction models were reconstructed under the guidance of URV and ICV. This study provided a method of predicting TVB-N content of pork in both spectral and imaging aspect for online testing the freshness of chilled pork, which improves the efficiency and quality of testing. TVBN TVB N completely detection nearinfrared near infrared GA SPA iPLSR images obtained accuracy 600750 600 750 600-75 nm 10201120 1020 1120 1020-112 14501570 1450 1570 1450-157 60075 60 75 600-7 1020112 102 112 1020-11 1450157 145 157 1450-15 6007 6 7 600- 102011 10 11 1020-1 145015 14 15 1450-1 10201 1 1020- 14501 1450-

Abstract Background: G protein coupled receptor kinase 2 (GRK2) has been demonstrated to play a crucial role in the development of chronic pain. Acupuncture is an alternative therapy widely used for pain management. In this study, we investigated the role of spinal neuronal GRK2 in electroacupuncture (EA) analgesia. Methods: The mice model of inflammatory pain was built by subcutaneous injection of Complete Freund's Adjuvant (CFA) into the plantar surface of the hind paws. The mechanical allodynia of mice was examined by von Frey test. The mice were subjected to EA treatment (BL60 and ST36 acupuncture points) for 1 week. Overexpression and down-regulation of spinal neuronal GRK2 were achieved by intraspinal injection of adeno associated virus (AAV) containing neuron–specific promoters, and microglial activation and neuroinflammation were evaluated by real–time PCR. Results: Intraplantar injection with CFA in mice induced the decrease of GRK2 and microglial activation along with neuroinflammation in spinal cord. EA treatment increased the spinal GRK2, reduced neuroinflammation, and significantly decreased CFA–induced mechanical allodynia. The effects of EA were markedly weakened by non–cell–specific downregulation of spinal GRK2. Further, intraspinal injection of AAV containing neuron–specific promoters specifically downregulated neuronal GRK2, and weakened the regulatory effect of EA on CFA–induced mechanical allodynia and microglial activation. Meanwhile, overexpression of spinal neuronal GRK2 decreased mechanical allodynia. All these indicated that the neuronal GRK2 mediated microglial activation and neuroinflammation, and subsequently contributed to CFA–induced inflammatory pain. Conclusion: The restoration of the spinal GRK2 and subsequent suppression of microglial activation and neuroinflammation might be an important mechanism for EA analgesia. Our findings further suggested that the spinal GRK2, especially neuronal GRK2, might be the potential target for EA analgesia and pain management, and we provided a new experimental basis for the EA treatment of pain.

Abstract Objective: The purpose of this study was to explore the clinical effect of preoperative health education based on the WeChat platform for parents of children with simple congenital heart disease. Methods: In this study, participants were randomly divided into an intervention group (WeChat group, n=40) and a control group (leaflet group, n=40) in our center. All parents were required to complete the Family Caregiver Task Inventory (FCTI) on the first visit and the Zarit Burden Interview (ZBI) and FCTI before the operation. Clinical, family and relevant data from children and parents were collected and subsequently analyzed. Results: Before the operation, the FCTI score and the ZBI score in the WeChat group were significantly lower than those in the control group (P=0.010, P=0.027, respectively). Compared to the FCTI score on the first visit, the preoperative status score was significantly lower in the WeChat group (P=0.008). The rate of loss to follow-up and complications in the WeChat group was also significantly lower than in the control group (P=0.003). Conclusion: Preoperative health education assisted by the WeChat platform for parents of children with simple congenital heart disease can effectively improve the parents' care ability and reduce the burden of care, preoperative complications and the rate of loss to follow-up. Objective Methods n=40 n40 n 40 leaflet center (FCTI (ZBI operation Clinical analyzed Results P=0.010, P0010 P 0 010 (P=0.010 P0027 027 P=0.027 respectively. respectively . respectively) P=0.008. P0008 P=0.008 008 (P=0.008) followup follow up P=0.003. P0003 P=0.003 003 (P=0.003) Conclusion followup. up. n=4 n4 4 P=0.010 P001 01 (P=0.01 P002 02 P=0.02 P000 P=0.00 00 (P=0.008 (P=0.003 n= P=0.01 P00 (P=0.0 P=0.0 (P=0.00 P0 (P=0. P=0. (P=0 P=0 (P= P= (P

Abstract Objective: To investigate the effect of WeChat-based telehealth services on the postoperative follow-up of children who underwent congenital heart surgery during the COVID-19 epidemic. Methods: This study retrospectively analyzed the clinical and family data of 108 children who underwent congenital heart surgery and underwent remote follow-up via the WeChat platform from December 2019 to March 2020 in our hospital. Results: During the follow-up period, the WeChat platform was used to refer 8 children with respiratory infection symptoms to local hospitals for treatment. Two children with poor incision healing were healed after we used the WeChat platform to guide the parents in dressing the wounds on a regular basis at home. Nutritional guidance was given via the WeChat platform to 13 patients with poor growth and development. The psychological evaluation results of the parents showed that the median (range) SDS score was 43 (34-59), and 7 parents (6.5%) were classified as depressed; the median (range) SAS score was 41 (32-58), and 12 parents (11.1%) were classified as having mild anxiety. Conclusion: The use of WeChat-based telehealth services was effective for the remote postoperative follow-up of children who underwent congenital cardiac surgery during the COVID-19 epidemic. Providing WeChat-based telehealth services can reduce the amount of travel required for these children and their families, which is helpful for controlling and preventing the spread of COVID-19. Objective WeChatbased based followup follow up COVID19 COVID 19 COVID-1 epidemic Methods 10 201 202 hospital Results period treatment home 1 development range (range 4 3459, 3459 34 59 , (34-59) 6.5% 65 6 5 (6.5% depressed 3258, 3258 32 58 (32-58) 11.1% 111 11 (11.1% anxiety Conclusion families COVID19. 19. COVID1 COVID- 20 345 3 (34-59 6.5 (6.5 325 (32-58 11.1 (11.1 2 (34-5 6. (6. (32-5 11. (11. (34- (6 (32- (11 (34 ( (32 (1 (3

Abstract Objective: To analyze and summarize the clinical safety and feasibility of minimally invasive video-assisted mitral valve replacement via a right thoracic minimal incision in patients aged over 65 years. Methods: The clinical data of 45 patients over 65 years old who had mitral valve disease were analyzed retrospectively from January 2014 to January 2017 at Union Hospital, Fujian Medical University. The patients were divided into two groups; 20 patients in group A, who underwent minimally invasive video-assisted mitral valve replacement via a right thoracic minimal incision, and 25 patients in group B, who underwent conventional mitral valve replacement. We collected and analyzed their relevant clinical data. Results: The operation was completed successfully in both groups. Compared with group B, group A was clearly superior for postoperative analgesia time, postoperative hospital length of stay, thoracic drainage liquid, blood transfusion, and length of incision. There were no differences between the two groups in postoperative severe complications and mortality. More patients in group B had pulmonary infections and poor incision healing, while more patients in group A had postoperative pneumothorax and subcutaneous emphysema. Conclusion: In patients aged over 65 years, minimally invasive video-assisted mitral valve replacement with a small incision in the right chest had the same clinical safety and efficacy as the conventional method.