ABSTRACT The Cerambycidae Oncideres impluviata (Germar, 1823) is an important insect pest for Acacia mearnsii De Wild in Southern Brazil. The damage caused by their girdling behavior reduces tree productivity, specially in the early years of plant establishment, when girdling is performed on the main trunk of trees. Here, we used a fragment of the mtDNA COI gene to analyze the genetic diversity, population structure and demography of O. impluviata in Southern Brazil, as well as to present the first hypothesis of phylogenetic relationships among species of the genus Oncideres. Our results identified five distinct haplotypes among the populations of O. impluviata, with the most common haplotype identified as O.imp_COI_01. The phylogenetic inferences corroborated the monophyly of O. impluviata with maximum statistical support. In addition, the phylogeny recovered three main population strains that are largely congruent with the haplotype network, which includes two lineages that are found in different edaphic regions of Rio Grande do Sul (Serra do Sudeste and Encosta Inferior do Nordeste). This is the first molecular phylogenetic assessment of O. impluviata. Our findings provide insights into the evolution of a significant species for the Brazilian forestry sector, as well as new resources for planning of pest management strategies.

Resumo O controle biológico é uma importante ferramenta para o controle de insetos-praga pelas inúmeras vantagens apresentadas e por diminuir os riscos ambientais provocados pelas técnicas de controle químico. No entanto, são escassos estudos referentes à morfofisiologia de microrganismos utilizados nesse método de controle, principalmente no que se refere ao Oncideres impluviata, principal inseto-praga da acácia-negra. Assim, o objetivo do presente estudo foi realizar a caracterização morfofisiológica de isolados de Beauveria bassiana e Metarhizium anisopliae, como uma potencial alternativa para o controle biológico do anelador-da-acácia-negra. Para tanto, foram utilizados cinco isolados de fungos entomopatogênicos (três isolados de Beauveria bassiana e dois de Metarhizium anisopliae) e avaliados o crescimento micelial, esporulação, coloração e caracterização dimensional dos conídios em diferentes meios de cultura (BDA, V8CaCO3 e meio completo). Os isolados de Beauveria bassiana apresentaram melhor crescimento micelial no meio V8CaCO3 e maior esporulação no meio de cultura completo; o micélio aéreo se apresentou de cor branca em todos os isolados. Em relação aos isolados de Metarhizium anisopliae, o maior crescimento micelial e esporulação ocorreram no meio de cultura completo e a coloração do micélio aéreo variou entre tons de cinza e verde-oliva. Esses resultados vêm a gerar informações preliminares a respeito do uso de fungos entomopatogênicos para o controle do anelador-da-acácia-negra.

Abstract Biological control is a useful tool for insect pests control due to the many advantages set out and further to reduce the environmental risks caused by chemical control techniques. However, there is a gap in the scientific literature that describes microorganisms' morphophysiology used in this control method, especially in Oncideres impluviata, the main pest insect of black wattle (Acacia mearnsii De Wild.). Thus, this study aimed to perform morphophysiology characterization of isolates of Beauveria bassiana and Metarhizium anisopliae as a potential alternative for the biological control of twig girdler of black wattle. So, five isolates of entomopathogenic fungi (three isolates of Beauveria bassiana and two of Metarhizium anisopliae) were used. Also, mycelial growth, sporulation, staining was evaluated. Additionally, a dimensional characterization of conidia in different culture media (BDA, V8CaCO3, and complete medium) was carried out. Beauveria bassiana isolates showed better mycelial growth in the V8CaCO3 medium and greater sporulation in the complete culture medium; the aerial mycelium presented a white color in all isolates. These results allow producing preliminary information regarding the use of entomopathogenic fungi for the control of the twig girdler of black wattle.

ABSTRACT Forest species can have their seeds damaged by granivorous insects, especially by those in their larval stage. In this context, this study aims to report the occurrence of Amblycerus species in Cordia trichotoma seeds, to describe their main damage to seeds and effects on germination, as well as their associated hymenopteran parasitoids. Therefore, seven trees were selected in the municipality of Taquaruçu do Sul, RS, Brazil. Fruits were collected weekly from the medium third of the tree crown, from the beginning of their formation until total dehiscence. To examine the damage caused by granivorous insects within the fruits, 15 fruits from each tree were sectioned with a scalpel. Furthermore, 10 fruits from each tree were stored individually in clear plates to verify the occurrence and identification of granivorous insect species. Evidence of the damage caused to seeds was verified through the germination test by comparing preserved and damaged seeds, with four repetitions of 25 seeds each. The species Amblycerus longesuturalis and Amblycerus profaupar (Chrysomelidae: Bruchinae) were found associated with fruits of C. trichotoma. Female insects predominantly laid eggs on the superior part between the marcescent calyx and the fruit, and larvae perforated the fruit tegument to start consuming seed embryos and reserves. Bruquine larvae are parasitized by Hymenoptera of Bracon, Mirax, Omeganastatus and Triapsis genera. In conclusion, the germination of C. trichotoma seeds is significantly affected by emergence orifices caused by granivorous species.

ABSTRACT This work aimed to characterize the composition of the soil macrofauna in non-mined and mined areas recovered with native vegetation, Eucalyptus dunnii Maiden and Acacia mearnsii De Wild. plantations, in a coal mine. Five areas were evaluated, namely, three non-mined areas and two mined and recovered areas. Six soil samples per area were collected using a 10x10 cm circular probe, every month during one year. Defragmentation of the soil and removal of specimens was carried out in a laboratory. A total of 3,195 specimens from 11 taxa were collected. The most abundant groups were Hymenoptera, Isoptera and Coleoptera. There were significant correlations (p<0.05) between the total abundance of Isoptera and Coleoptera with some meteorological variables. The recovery process of the mined areas with black-wattle and eucalyptus plantations proved to be efficient because there was similarity of macrofauna groups between the non-mined, mined and native vegetation areas.

Abstract Heteropsylla caldwelli Burckhardt (Psyllidae, Ciriacreminae) is reported for the first time from Brazil (States of Minas Gerais, Paraná, Rio Grande do Sul) from Enterolobium contortisiliquum (Vell.) Morong. The earpod tree, from Albizia edwallii (Hoehne) Barneby and J.W. Grimes and Senegalia polyphylla (DC.) Britton (Fabaceae, Mimosoideae), all previously unknown hosts. The population dynamics of the psyllid were investigated in a seven-year-old plantation of E. contortisiliquum in an abandoned open-pit coal mine in Candiota, Rio Grande do Sul during two years. The population showed peaks in spring and summer, correlating directly with the mean air temperature and the population size of microhymenoptera.

Resumo: O objetivo deste trabalho foi avaliar a composição da mesofauna do solo em áreas não mineradas e mineradas, sob recuperação ambiental com vegetação nativa e silvicultura, em mina de carvão mineral no Sul do Brasil. Cinco tratamentos foram avaliados: vegetação nativa em área não minerada; plantação de Acacia mearnsii em área não minerada; plantação de A. mearnsii em área minerada; plantação de Eucalyptus dunnii em área não minerada; e plantação de E. dunnii em área minerada. Durante um ano, quatro amostras de solo foram retiradas mensalmente, por tratamento. Os organismos foram extraídos com uso de funil de Berlese e luz incandescente de 50 W. Foram coletados 23.911 organismos da mesofauna do solo, identificados e distribuídos em 13 grupos taxonômicos. A abundância de espécimes foi similar entre os tratamentos. A maior diversidade de grupos taxonômicos foi encontrada sob plantação de A. mearnsii, em área minerada, e nas plantações de E. dunnii em área não minerada e minerada, todas com 13 grupos. Eucalyptus dunnii e A. mearnsii auxiliam na mitigação dos impactos da mineração de carvão sobre a diversidade dos grupos taxonômicos da mesofauna do solo, pois a composição dos mesoartrópodes é semelhante à das áreas que não foram diretamente afetadas pelos efeitos da mineração.

Abstract: The objective of this work was to evaluate the soil mesofauna composition in non-mining and in mining areas under environmental recovery with native vegetation and forest planting, in a coalmine in Southern Brazil. Five treatments were evaluated: native vegetation in non-mining area; plantation of Acacia mearnsii in non-mining area; plantation of A. mearnsii in mining area; plantation of Eucalyptus dunnii in non-mining area; and plantation of E. dunnii in mining area. Four soil samples per treatment were collected monthly, during one year. The organisms were extracted using a Berlese funnel with 50-W incandescent light. A total of 23,911 organisms were collected from the soil mesofauna, which were identified and distributed into 13 taxonomic groups. The abundance of specimens was similar between treatments. The greatest diversity of taxonomic groups was found under A. mearnsii plantation, in the mining area, and under E. dunnii plantations in non-mining and mining areas, all with 13 groups. Eucalyptus dunnii and A. mearnsii help to mitigate the impact of coal mining on the diversity of the soil mesofauna taxonomic groups, since the composition of mesoarthropods is similar to that of areas that have not been directly affected by mining.

RESUMO O estudo objetivou avaliar a eficiência de inseticidas no controle de Thaumastocoris peregrinus em laboratório. Para tanto, 15 plantas de Eucalyptus dunnii receberam aplicações a campo de: imidacloprido - 78,8 (g/ha), tiametoxam - 37,5 (g/ha), lambda-cialotrina+tiametoxam - 21,2+25,8 (g/ha), lambda-cialotrina - 24,9 (g/ha), e somente água (testemunha). Para determinar a eficiência dos inseticidas, utilizou-se o teste de confinamento, onde folhas de E. dunnii foram pulverizadas uma única vez, e em seguida, coletadas em dois períodos distintos. Em laboratório, foram utilizadas oito folhas para ninfas e oito para adultos, por tratamento, individualizadas em placas de petri; cada repetição recebeu 10 exemplares da espécie. As avaliações da sobrevivência foram realizadas no segundo, quarto, vigésimo segundo e vigésimo quarto dias após a aplicação dos tratamentos (DAT). Conclui-se que os inseticidas testados, nas suas respectivas doses, apresentam eficiência de controle de ninfas e adultos de T. peregrinus.

ABSTRACT The study aimed to evaluate the control efficiency of insecticides on Thaumastocoris peregrinus in the laboratory conditions. Therefore, 15 plants of Eucalyptus dunnii received the follow field spray: imidacloprid (78.8g ai / ha), thiamethoxam (37.5g ai / ha), lambda-cyhalothrin + thiamethoxam (21.2 + 25.8g ai / ha), lambda-cyhalothrin (24.9g ai / ha), and the untreated control only water. To determine the insecticides efficiency, we used a containment test, with E. dunnii leaves sprayed and collected at two different times. In the laboratory was used eight reps of leaves to adult and eight to nymphs by treatment individually placed in petri dishes; each repetition received 10 insect specimens. Assessments of alive insects were carried out at second, fourth, twentieth, twenty-fourth days after spray treatment (DAT). We conclude that the insecticides tested, on respective rate, presented efficient control of T. peregrinus nymphs and adults.

Leptoglossus stigma, Leptoglossus zonatus (Hemiptera: Coreidae) y Loxa deducta (Hemiptera: Pentatomidae), fueron encontrados atacando frutos de Carya illinoinensis (Junglandaceae), en dos plantaciones con diez años de edad, localizadas en áreas del municipio de Paraíso do Sul, Rio Grande do Sul, Brasil. Los registros fueron realizados en marzo de 2014 para las especies del género Leptoglossus y en marzo de 2015, para Loxa deducta. Este es el primer registro de estas especies de chinches Coreidae y Pentatomidae consumiendo frutos de nogal pecanero en el país.

Leptoglossus stigma, Leptoglossus zonatus (Hemiptera: Coreidae) and Loxa deducta (Hemiptera: Pentatomidae), have been found attacking Carya illinoinensis (Junglandaceae) fruit, in two 10-year-old plantings, located in areas in the city of Paraíso do Sul, Rio Grande do Sul, Brazil. The record was made in March, 2014, concerning the species from genus Leptoglossus and, in March, 2015, Loxa deducta. This represents, therefore, the first record of these Coreidae and Pentatomidae species bugs-feed on pecan tree fruit in the country.

Este estudo teve como objetivo avaliar os efeitos de diferentes tipos de controle de plantas infestantes sobre a mirmecofauna em um plantio inicial de Eucalyptus grandis. Para tanto, em março de 2011 foi realizado o início da implantação da cultura, em uma área localizada no município de Santa Maria - RS. Os seis tratamentos constituíram-se em controle químico total de plantas infestantes, na linha e na entrelinha de plantio, com glifosato (T1); controle químico total de plantas infestantes, na linha de plantio, com glifosato (T2); controle químico de monocotiledôneas na linha e entrelinha de plantio, com setoxidim (T3); controle químico de dicotiledôneas na linha e entrelinha de plantio, com bentazona (T4); controle químico total de plantas infestantes em faixa de um metro paralela à linha de plantio, com glifosato, e de um metro na parte central da entrelinha, sem controle (T5); e testemunha, sem controle de plantas infestantes (T6). O levantamento da mirmecofauna foi realizado no período de um ano utilizando-se três métodos de coleta: isca atrativa, armadilha de solo e funil de Berlese, com seis repetições por tratamento, em cada data de coleta. Nesse período foram coletadas 46.675 formigas, distribuídas em 37 espécies, não sendo verificada diferença significativa entre o total de espécimens coletados. Na área do tratamento constituído pelo controle de plantas infestantes somente na linha de plantio verificou-se eficiência amostral de 99,0% e S obs = 35. Entre os índices de Diversidade de Shannon (H'), destacou-se o valor encontrado para a área do tratamento T2 (H'= 1,34) em detrimento dos valores das áreas nas quais foram instalados os tratamentos T1 (H'= 1,25) e T5 (H'= 1,23). Havendo assim, uma maior coexistência de espécies de formigas entre as áreas com estrutura florística menos alterada e entre as áreas mais simplificadas. Não tendo sido verificada correlação significativa (r = 0,0463) entre a riqueza de espécies de formigas coletadas e o número de plantas infestantes encontradas ao final do experimento. Assim, conclui-se que os efeitos indiretos da ação dos herbicidas afetam mais a composição local de espécies de formigas do que sua riqueza.

The aim of this study was to evaluated the effects of different types of weed control on the ant fauna in an initial planting of Eucalyptus grandis. Therefore, in march of 2011 was performed the implantation of culture, in an area located in the city of Santa Maria - RS. The six treatments were constituted by the total chemical control of weeds in row and interrow planting, with glyphosate (T1); total chemical control of weeds in the crop row, with glyphosate (T2); chemical control of monocotyledonous in row and interrow planting, with sethoxydim (T3); chemical control of dicotyledonous line interrow and planting, with bentazone (T4); total chemical control of weeds in a yard track parallel to the row, with glyphosate, and a meter in the central part of interrow without control (T5); and without control weeds (T6). The survey of the ant fauna was conducted in one year using three collection methods: attractive bait, trap soil and Berlese funnel, with six replicates per treatment in each collection date. In this period were collected 46,675 ants, belonging to 37 species, there being no significant difference between the total specimens collected. In the area of treatment consists of the control of weeds in the crop row only verified efficiency of 99.0% and sample S obs = 35. Among the indices of Shannon Diversity (H'), highlighted the value found for the area of treatment T2 (H'= 1.34) at the expense of the values of the areas where they were installed the T1 (H'= 1,25) and T5 (H'= 1,23). Having thus a higher co-existence of ant species between the areas with changed structure less flora, and between the areas most simplified. There were significant correlation (r = 0.0463) between the species richness of ants collected and the number of weeds found at the end of the experiment. Thus, we conclude that the indirect effects of the herbicides affect more the local composition ant species than his richness.

O Eucalyptus grandis destaca-se como uma das espécies mais cultivadas no Brasil em razão de sua produtividade e da qualidade da madeira. Objetivou-se com este estudo avaliar a coleopterofauna edáfica em um plantio inicial de E. grandis sob diferentes sistemas de controle de plantas daninhas em Santa Maria, RS, utilizando-se armadilhas de solo. Os tratamentos avaliados basearam-se no controle químico de plantas daninhas: total, na linha e entrelinha de plantio (CT); total, na linha de plantio (CL); monocotiledôneas, na linha e entrelinha de plantio (CM); dicotiledôneas, na linha e entrelinha de plantio (CD); em faixas, com controle de um metro ao lado da linha de plantio, deixando-se um metro na entrelinha sem controle (CF); e sem controle (SC). No período de março de 2011 a agosto de 2012 foram coletados 1,190 coleópteros, distribuídos em sete famílias. Houve diferença entre a coleopterofauna edáfica capturada nos tratamentos avaliados. A aplicação de uma menor quantidade de herbicidas mantém a diversidade vegetal, possibilitando maior diversidade de coleópteros na área.

Eucalyptus grandis stands out as one of the most cultivated species in Brazil because of its productivity and wood quality. The objective of this study was to assess the edaphic coleopteran fauna in an initial planting of E. grandis under different systems of weed control in Santa Maria, Rio Grande do Sul state, using pitfall traps. The treatments were based on the chemical control of weeds: total, in and between planting rows (CT); total, in the planting row (CL); monocotyledons, in and inter-rows (CM); dicotyledons, in and inter-rows (CD); control of one meter next to the planting row, leaving one meter between rows without control (CF); and without control (SC) . From March 2011 to August 2012, 1190 beetles of seven families were collected. There were differences between the edaphic coleopteran fauna captured in the evaluated treatments. The application of a smaller amount of herbicide maintains plant diversity, allowing greater diversity of beetle fauna in the area.