Nanocomposites based on superhydrophilic vertically aligned multi-walled carbon nanotubes (VAMWCNT-O2) and nanohydroxyapatite (nHAp) are of great interest in bone regenerative medicine. The biomineralization using simulated body fluid (SBF) has been extensively studied to evaluate the bioactivity of biomaterials. Thus, the combination of nHAp and VAMWCNT-O2 is attractive and promising. The aim of this study was to evaluate the in vitro calcification of nHAp/VAMWCNT-O2 nanocomposites before and after the period of biomineralization in SBF. In vitro calcification of the extracellular matrix (ECM) of HOB cells in culture after 24 hours was investigated through the assay of alkaline phosphatase. These promising in vitro results validate biomineralized nHAp/VAMWCNT-O2 as possible scaffolds for bone tissue regeneration.

Neste estudo investigamos o efeito da irradiação da lâmpada infravermelha durante o processo de fadiga muscular. Foram utilizados 3 grupos diferentes (n=5), sendo 1 grupo controle e 2 irradiados (Lâmpada de Infravermelho 780-1400nm), nas densidades de energia 0,5 e 1,0J/cm² e tempo de irradiação 300 segundos. Os animais irradiados receberam 1 irradiação em 1 ponto, diretamente no músculo tibial, após a primeira contração tetânica de um total de seis, com um intervalo entre cada tetania. Os resultados foram registrados em eletrofisiógrafo e analisado a intensidade da força de contração em gramas. Foi observado que o grupo controle apresentou uma redução na intensidade da força de contração, já os grupos irradiados conseguiram mantê-la, sendo mais evidente quando irradiado com densidade de energia (DE) de 0,5J/cm². Conclui-se que a utilização da irradiação da lâmpada infravermelha é eficaz na resistência a fadiga muscular.

In this study the effects of infrared lamp illumination during the muscle fatigue process was studied. Three different groups (n=5) were used: one control group and two treated (Infrared Lamp 780-1400nm), with the energy densities of 0.5 and 1.0 J/cm² and time of illumination of 300 seconds. The treated animals were illuminated in one point directly in the tibialis muscle, after the first tetanic contraction out of six, with an interval between each tetany. The results were registered in an electrophysiograph and the intensity of the force of contraction in grams was analysed. It was observed that the control group presented a reduction in the intensity of the force of contraction, while the treated group managed to maintain it, which was clearly evident in the energy density of 0.5 J/cm². It was concluded that the use of the infrared lamp illumination was efficient concerning resistance to muscle fatigue.

The effects of Photodynamic Therapy using 2nd generation photosensitizers have been widely investigated aiming clinical application treatment of solid neoplasms. In this work, ultrastructure changes caused by the action of two 2nd generation photosensitizers and laser irradiation on CHO-K1 and HeLa (neoplastic) cells were analyzed by transmission electron microscopy. Aluminum phthalocyanine chloride, aluminum phthalocyanine tetrasulfonate chloride and radiation from a semiconductor laser at a fluency of 0.5 J/cm² (Power=26mW; l=670nm) were used. The results showed induction of apoptosis. Such alterations where observed in HeLa but not in CHO-K1 cells after Aluminum phthalocyanine tetrasulfonate chloride (AlPcS4) photodynamic treatment. The Aluminum phthalocyanine chloride (AlPc) photodynamic treatment induced necrosis on the neoplastic cell line, and cytoplasm and nuclear alterations on the normal cell line.