Abstract Specific products containing natural resources can contribute to the innovation of complete denture hygiene. Objective: To conduct an in vitro evaluation of experimental dentifrices containing essential oils of Bowdichia virgilioides Kunth (BvK), Copaifera officinalis (Co), Eucalyptus citriodora (Ec), Melaleuca alternifolia (Ma) and Pinus strobus (Ps) at 1%. Methodology: The variables evaluated were organoleptic and physicochemical characteristics, abrasiveness (mechanical brushing machine) simulating 2.5 years, and microbial load (Colony Forming Units - CFU/mL), metabolic activity (XTT assay) and cell viability (Live/Dead® BacLight™ kit) of the multispecies biofilm (Streptococcus mutans: Sm, Staphylococcus aureus: Sa, Candida albicans: Ca and Candida glabrata: Cg). Specimens of heat-polymerized acrylic resins (n=256) (n=96 specimens for abrasiveness, n=72 for microbial load count, n=72 for biofilm metabolic activity, n=16 for cell viability and total biofilm quantification) with formed biofilm were divided into eight groups for manual brushing (20 seconds) with a dental brush and distilled water (NC: negative control), Trihydral (PC: positive control), placebo (Pl), BvK, Co, Ec, Ma or Ps. After brushing, the specimens were washed with PBS and immersed in Letheen Broth medium, and the suspension was sown in solid specific medium. The organoleptic characteristics were presented by descriptive analysis. The values of density, pH, consistency and viscosity were presented in a table. The data were analyzed with the Wald test in a generalized linear model, followed by the Kruskal-Wallis test, Dunn's test (mass change) and the Bonferroni test (UFC and XTT). The Wald test in Generalized Estimating Equations and the Bonferroni test were used to analyze cell viability. Results: All dentifrices showed stable organoleptic characteristics and adequate physicochemical properties. CN, Ec, Ps, Pl and PC showed low abrasiveness. There was a significant difference between the groups (p<0.001) for microbial load, metabolic activity and biofilm viability. Conclusions: It was concluded that the BvK, Ec and Ps dentifrices are useful for cleaning complete dentures, as they have antimicrobial activity against biofilm. The dentifrices containing Bowdichia virgilioides Kunth showed medium abrasiveness and should be used with caution. hygiene Objective BvK , (BvK) Co (Co) (Ec) (Ma (Ps 1 1% Methodology mechanical machine 25 2 5 2. years Colony CFU/mL, CFUmL CFU/mL CFU mL CFU/mL) XTT assay Live/Dead® LiveDead Live Dead (Live/Dead BacLight kit Streptococcus mutans Sm aureus Sa albicans glabrata Cg. Cg . Cg) heatpolymerized heat polymerized n=256 n256 n 256 (n=256 n=96 n96 96 (n=9 n72 72 n=7 count n16 16 n=1 quantification 20 (2 seconds NC (NC control, control control) (PC Pl, (Pl) analysis density pH table model KruskalWallis Kruskal Wallis Dunns Dunn s mass change UFC XTT. XTT) Results properties CN p<0.001 p0001 p 0 001 (p<0.001 Conclusions dentures caution (BvK (Co (Ec Live/Dead n=25 n25 (n=25 n=9 n9 9 (n= n7 7 n= n1 ( (Pl p<0.00 p000 00 (p<0.00 n=2 n2 (n=2 (n p<0.0 p00 (p<0.0 p<0. p0 (p<0. p<0 (p<0 p< (p< (p

Abstract Appropriated denture hygiene is a predictive factor for longevity of rehabilitation treatment and maintenance of the oral mucosal health. Although, disinfectant solutions are commonly used as denture cleansers, the impact of these solutions on acrylic resin-based dentures remain unclear. Objective To evaluate, in vitro, the antibiofilm activity of complete denture hygiene solutions and their effects on physical and mechanical properties of acrylic resin. Methodology For antibiofilm activity measurement acrylic resin specimens were contaminated with Candida albicans, Candida glabrata and Streptococcus mutans. After biofilm growth, the specimens were assigned to the hygiene solutions: Distilled water (Control); 0.2% Sodium hypochlorite (SH); Efferdent Power Clean Crystals (EPC) and 6.25% Ricinus communis (RC). The viability of microorganisms was evaluated by agar plate counts. In parallel, physical, and mechanical properties of the acrylic resin were evaluated after simulating a 5-year period of daily immersion in the previously mentioned solutions. The changes in surface roughness, color, microhardness, flexural strength, impact strength, sorption and solubility were evaluated. Data were compared by ANOVA followed by the Tukey test or Kruskal-Wallis followed by the Dunn test depending on the distribution (α=0.05). Results Regarding antibiofilm action, SH eliminated all microorganisms while EPC and RC exhibited moderate action against S. mutans (p=0.001) and C. glabrata (p<0.001), respectively. Relative to effects on the physical and mechanical properties of the acrylic resin, RC led to higher values of color change (p=0.030), hardness (p<0.001), surface roughness (p=0.006) and flexural strength (p<0.001). Moreover, RC induced the highest values of changes in solubility (p<0.001). EPC promoted greater changes in surface morphology, whereas immersion in SH retained the initial appearance of the acrylic resin surface. All hygiene solutions reduced the impact strength (p<0.05). Conclusion SH presented the most effective antibiofilm activity. In addition, changes on properties were observed after immersion in RC, which were considered within acceptable limits.

Abstract Understanding the behavior of Candida spp. when exposed to denture disinfectants is essential to optimize their effectiveness. Changes in the virulence factors may cause increased resistance of Candida spp. to disinfectant agents. Objective To evaluate the microbial load, cellular metabolism, hydrolytic enzyme production, hyphae formation, live cell and biofilm quantification of Candida albicans, Candida tropicalis and Candida glabrata after exposure to disinfectant solutions. Methodology Simple biofilms were grown on heat-polymerized acrylic resin specimens, and divided into groups according to solutions/strains: distilled water (control); 0.25% sodium hypochlorite (NaOCl 0.25% ); 10% Ricinus communis (RC 10%); and 0.5% Chloramine T (CT 0.5%). The virulence factors were evaluated using the CFU count (microbial load), XTT method (cell metabolism), epifluorescence microscopy (biofilm removal and live or dead cells adhered), protease and phospholipase production and hyphae formation. Data were analyzed (α=0.05) by one-way ANOVA/ Tukey post hoc test, Kruskal-Wallis test and Wilcoxon test. Results NaOCl 0.25% was the most effective solution. CT 0.5% reduced the number of CFUs more than RC 10% and the control. RC 10% was effective only against C. glabrata. RC 10% and CT 0.5% decreased the cellular metabolism of C. albicans and C. glabrata. Enzyme production was not affected. Hyphal growth in the RC 10% and CT 0.5% groups was similar to that of the control. CT 0.5% was better than RC 10% against C. albicans and C. tropicalis when measuring the total amount of biofilm and number of living cells. For C. glabrata, CT 0.5% was equal to RC 10% in the maintenance of living cells; RC 10% was superior for biofilm removal. Conclusions The CT 0.5% achieved better results than those of Ricinus communis at 10%, favoring the creation of specific products for dentures. Adjustments in the formulations of RC 10% are necessary due to efficacy against C. glabrata. The NaOCl 0.25% is the most effective and could be suitable for use as a positive control.

RESUMO O sistema CAD-CAM (computer aided design/computer aided manufacturing), que significa desenho auxiliado por computação e manufatura auxiliada por computação, é um dos recursos mais tecnológicos e modernos na reabilitação oral atualmente. É uma alternativa que otimizou a resistência e a adaptação das cerâmicas odontológicas. Dessa maneira, suas indicações de uso têm-se ampliadas, tornando-o cada vez mais conhecido e difundido. O objetivo deste estudo é apresentar uma revisão da literatura sobre estudos comparativos das propriedades mecânicas dos sistemas cerâmicos produzidos por CAD/CAM. Foi realizada a busca de artigos científicos publicados entre 2009 e 2019, em inglês, espanhol ou português, nas bases de dados SCIELO, BIREME e PUBMED, utilizando os descritores: “Ceramics”, “Computer Aided Design” e “Prótese Parcial Fixa”. Atualmente todos os sistemas cerâmicos parecem ter uma adequada resistência para reabilitações simples, mas as restaurações cerâmicas produzidas por sistemas CAD/CAM apresentam maior confiabilidade do que outros métodos de fabricação; apresentando maiores possibilidades de indicações devido aos seus maiores valores de resistência mecânica. Esta tecnologia embora apresente o custo como um fator limitante, está em pleno desenvolvimento e já apresenta propriedades mecânicas suficientes e com índices de sucesso que a qualificam como o estado da arte em reabilitação oral.

ABSTRACT Computer aided design / computer aided manufacturing (CAD/CAM) systems are among the most technological advanced techniques in oral rehab today. Compared with conventional techniques, they can optimize the resistance and the adaptation of dental ceramics. Thus, their indications of use have been widened, making the technique more and more well-known and widespread. Therefore, the aim of this study is to present a literature review on comparative studies of the mechanical properties of ceramic systems produced by CAD / CAM. A search for scientific articles published between 2009 and 2019, in English, Spanish or Portuguese, was performed through the databases SCIELO, BIREME and PUBMED, using the descriptors “Ceramics”, “Computer Aided Design” and “ Partial Fixed Prosthesis “. Currently, all ceramic systems appear to have adequate strength for simple rehabilitations, but ceramic restorations produced by CAD/CAM systems present greater reliability than other manufacturing methods, presenting a wider array of indications due to their higher mechanical resistance. These systems, besides their versatility, also present an excellent aesthetic result, guaranteeing appropriate optical properties such as translucency and fluorescence, similar to natural teeth. Although the cost is a limiting factor, CAD/CAM technology is in full development and with high success rates that qualifies it as the state of art in oral rehabilitation.

Resumo Este estudo avaliou a eficácia de soluções higienizadoras na remoção do biofilme de dentadura por meio de ensaio clínico randomizado cruzado. Trinta e dois pacientes desdentados foram instruídos a escovar suas dentaduras (escova específica e sabão líquido) três vezes ao dia (após café da manhã, almoço e jantar) e imergi-las (≥ 8 horas) em: (C) controle - água; (PA): peróxido alcalino; ou (HS) hipoclorito de sódio a 0,5%. Cada solução foi usada por 21 dias (três ciclos alternados de 7 dias). Ao final de cada ciclo, a superfície interna da dentadura maxilar foi evidenciada (vermelho neutro 1%) e fotografada (HX1- Sony). As áreas (total e corada com biofilme) foram medidas (software Image Tool), e a porcentagem de biofilme calculada como a relação entre a área do biofilme multiplicado por 100 e área da superfície total da base interna da dentadura. Os dados foram comparados por meio de equações de estimação generalizadas (α=5%) e comparações múltiplas (Bonferroni - α=1,67%). A imersão em HS reduziu o biofilme (%) (8,3 ± 13,3B) em comparação com C (18,2 ± 14,9A) e PA (18,2 ± 16,6A). A solução de hipoclorito de sódio a 0,5% foi a mais eficaz na remoção do biofilme. Peróxidos alcalinos podem não levar a maior remoção do biofilme em pacientes com hábitos adequados de manutenção de dentadura.

Abstract This study evaluated the efficacy of cleanser solutions on denture biofilm removal by a crossover randomized clinical trial. Thirty two edentulous patients were instructed to brush their dentures (specific brush and liquid soap) three times a day (after breakfast, lunch and dinner) and to soak them (≥ 8 h) in: (C) control -water; (AP): alkaline peroxide; or (SH) 0.5% sodium hypochlorite. Each solution was used for 21 days (three cycles of 7 days). At the end of each cycle, the inner surfaces of maxillary dentures were disclosed (1% neutral red) and photographed (HX1 - Sony). Areas (total and stained biofilm) were measured (Image Tool software) and the percentage of biofilm calculated as the ratio between the area of the biofilm multiplied by 100 and total surface area of the internal base of the denture. Data were compared by means of generalized estimating equation (α=5%) and multiple comparisons (Bonferroni; α=1.67%). Immersion in SH reduced biofilm (%) (8.3 ± 13.3B) compared to C (18.2 ± 14.9A) and AP (18.2 ± 16.6A). The 0.5% sodium hypochlorite solution was the most efficacious for biofilm removal. Alkaline peroxides may not lead to further biofilm removal in patients with adequate denture maintenance habits.

ABSTRACT To preserve oral health and to maintain the prosthetic devices, it is important not only to improve the properties of commonly known hygiene products, but also to investigate new materials with antimicrobial action. Objectives This study evaluated the antimicrobial activity of sodium hypochlorite (0.25% and 0.50%) and 10% Ricinus communis’ solutions against specific microorganisms. Material and Methods Sixty four maxillary complete denture wearers were instructed to brush their dentures three times a day and to soak them (20 min/day) in the solutions: SH1: 0.25% sodium hypochlorite; SH2: 0.5% sodium hypochlorite; RC: 10% R. communis oil; and C: 0.85% saline (control). The solutions were used for 7 days in a randomized sequence. Following each period of use, there was a 1-week washout period. Antimicrobial activity was determined by Colony Forming Units (CFU) counts of Streptococcus mutans, Candida spp., and gram-negative microorganisms. For collecting biofilm, the internal surface of maxillary dentures was brushed with saline solution, and biofilm suspension obtained. After dilutions (100 - 10-3), aliquots were seeded in Mitis salivarius, CHROMagar Candida®, and MacConkey agar for detecting S. mutans, Candida spp., or gram-negative microorganisms, respectively. After incubation, colonies were counted, and CFU/mL values were calculated. Then, transformation - log10 (CFU+1) - data were analyzed using the Friedman test (α=0.05). Results showed significant differences between the solutions (p<0.001). Results All three solutions showed antimicrobial activity against S. mutans. Against Candida spp., RC and SH1 solutions showed similar effect while SH2 showed superior activity. SH1 and SH2 solutions showed antimicrobial action against gram-negative microorganisms. The Candida species most frequently isolated was C. albicans, followed by C. tropicalis and C. glabrata. Conclusions The 0.5% sodium hypochlorite solution was the most effective and might be used to control denture biofilm. C. albicans was the most frequently isolated Candida sp.

<p>Este estudo avaliou as alterações de cor, rugosidade de superfície e resistência à flexão de resinas acrílicas após imersão em soluções de peróxido alcalino e hipoclorito alcalino, simulando um período de cinco anos de uso. Sessenta corpos de prova circulares (16 mm x 4 mm) e 60 retangulares (65 mm x 10 mm x 3,3 mm) de resina acrílica termicamente ativada (Lucitone 550) foram distribuídos (n=20) em três grupos de imersão (20 min): C1: água destilada; PA: solução com água morna e uma pastilha de peróxido alcalino; HS: solução de hipoclorito de sódio a 0,5%. A alteração de cor (∆E) foi determinada por meio de colorímetro e também calculada de acordo com unidades da National Bureau of Standards (NBS). Um rugosímetro foi utilizado para mensuração da rugosidade de superfície (μm) e a resistência à flexão (MPa) foi medida utilizando uma máquina universal de ensaios. Os dados foram avaliados pelo teste de Kruskal-Wallis seguido pelo teste de Dunn (estabilidade de cor e rugosidade de superfície) e análise de variância (ANOVA) a um fator seguida pelo teste de Bonferroni (resistência à flexão). Para todos os testes foi considerado α=0,05. PA {0,79 (0,66;1,42)} causou alterações de cor significativamente maiores que C1 {0,45 (0,37;0,57)} e HS {0,34 (0,25;0,42)}. Os valores médios de ∆E quantificados pela NBS foram classificados como "indiciais" para C1 (0,43) e SH (0,31), e "leves" para PA (0,96). O HS {-0,015 (-0,023;0,003)} acarretou maiores valores de ∆Ra que C1 {0,000 (-0,004;0,010)} e PA {0.000 (-0,009;0,008)} Não houve diferença estatística significante entre as soluções para a resistência à flexão (C1: 84,62±16,00, PA: 85,63±12,99, HS: 84,22±14,72). Concluiu-se que imersões em soluções de peróxido alcalino e hipoclorito de sódio simulando imersões diárias de 20 min por um período de cinco anos não causaram efeitos adversos clinicamente significantes sobre a resina acrílica termicamente ativada.</p>

<p>This study evaluated color stability, surface roughness and flexural strength of acrylic resin after immersion in alkaline peroxide and alkaline hypochlorite solutions, simulating a five-year-period of use. Sixty disc-shaped (16x4 mm) and 60 rectangular specimens (65x10x3.3 mm) were prepared from heat-polymerized acrylic resin (Lucitone 550) and assigned to 3 groups (n=20) of immersion (20 min): C1: distilled water; AP: warm water and one alkaline peroxide tablet; SH: 0.5% NaOCl solution. Color data (∆E) were determined by a colorimeter and also quantified according to the National Bureau of Standards units. A rugosimeter was used to measure roughness (μm) and the flexural strength (MPa) was measured using a universal testing machine. Data were evaluated by Kruskal-Wallis followed by Dunn tests (color stability and surface roughness) and by one-way ANOVA and Bonferroni test (flexural strength). For all tests was considered α=0.05. AP {0.79 (0.66;1.42)} caused color alteration significantly higher than C1 {0.45 (0.37;0.57)} and SH {0.34 (0.25;0.42)}. The mean ∆Ε values quantified by NBS were classified as "trace" for C1 (0.43) and SH (0.31) and "slight" for AP (0.96). SH {-0.015 (-0.023;0.003)} caused significantly higher ΔRa than the C1 {0.000 (-0.004;0.010)} and AP {0.000 (-0.009;0.008)} groups. There was no statistically significant difference among the solutions for flexural strength (C1: 84.62±16.00, AP: 85.63±12.99, SH: 84.22±14.72). It was concluded that immersion in alkaline peroxide and NaOCl solutions simulating a five-year of 20 min daily soaking did not cause clinically significant adverse effects on the heat-polymerized acrylic resin.</p>

Este estudo avaliou a abrasividade causada pelos métodos mecânico e combinado de higiene de próteses totais, por meio da análise da perda de massa e rugosidade de superfície. Corpos de prova de resina acrílica (Plexiglass) foram escovados em máquina de escovação (Mavtec) com escova macia (Tek) e água ou dentifrícios (Sorriso, Colgate, Polident e Corega) (Grupos Experimentais) por 50 min, simulando 1 ano de escovação (método mecânico). Após a escovação, os corpos de prova foram imersos em hipoclorito de sódio a 0,5%, simulando imersões diárias de 20 min por 1 ano (método combinado). Água destilada (23 °C) foi empregada como controle. A perda de massa foi analisada por meio de balança analítica de precisão (g) e a rugosidade de superfície por rugosímetro (μm). Os dados foram submetidos à ANOVA e teste de Bonferroni (α=0,05). Em relação à perda de massa, os menores valores foram obtidos para o Polident em ambos os métodos (mecânico: -0,0072±0,0017; combinado: -0,011±0,002) e o método combinado resultou em maior perda de massa que o método mecânico, exceto para o Corega. Para a rugosidade de superfície, após aplicação do método mecânico, os valores mais baixos foram registrados para água (-0,007±0,016) e Polident (0,402±0,378); para o método combinado, os valores mais baixos foram obtidos para água (-0,063±0,013); não houve diferença estatística entre os métodos. Conclui-se que o Polident foi o dentifrício menos abrasivo e que a associação do método químico ao método mecânico aumentou a perda de massa, porém não alterou a rugosidade de superfície dos corpos de prova.

This study evaluated the abrasiveness of mechanical and combined methods of denture hygiene, by the analysis of mass loss and surface roughness. Acrylic resin specimens (Plexiglass) were brushed by a tooth brushing machine (Mavtec) with a soft brush (Tek) and water (control) or four dentifrices (Sorriso, Colgate, Polident and Corega) (Experimental groups) for 50 min, representing one year of brushing (mechanical method). After brushing, the specimens were immersed in 0.5% sodium hypochlorite simulating a daily cleaning of 20 min for one year (combined method). Distilled water (23 ºC) was employed as control. The mass loss (g) was analyzed by an analytical balance and the surface roughness (μm) by a rugosimeter. Data were subjected to ANOVA and Bonferroni test (α=0.05). Polident dentifrice showed lowest values of mass loss for both methods (mechanical: -0.0072±0.0017, combined:-0.011±0.002) and the combined method resulted in greater mass loss than the mechanical, except for Corega. For the surface roughness, after the mechanical method, the lowest values were registered for water (-0.007±0.016) and Polident (0.402±0.378); for the combined method, water (-0.063±0.013) showed the lowest values; there was no statistically significant difference between methods. It was concluded that Polident was the less abrasive dentifrice and the association between chemical and mechanical methods increased the mass loss but did not change the surface roughness of specimens.

The objective of this in vitro study was to evaluate the antimicrobial action of sodium hypochlorite (0.25% and 0.50%) and 10% castor oil solutions against specific microorganisms, by counting Colony Forming Units (CFU) of clinically important bacteria and Candida species. Acrylic resin specimens (n = 320; Lucitone 550) were obtained from square metal matrices (10 x 10 x 2 mm), sterilized by microwave (650W, for 6 minutes) and contaminated by Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, Bacillus subtilis, Escherichia coli, Streptococcus mutans, Enterococcus faecalisand Candida glabrata. The specimens were immersed for 20 minutes in one of the following hygiene solutions (n = 10/each): A – 0.25% Sodium hypochlorite; B – 0.5% Sodium hypochlorite; C – 10% Castor oil solution; and D (Control) – saline. Adhered cells were suspended and inoculated into a selective solid medium (37ºC for 24 h). The Student’s t-test (α = 0.05) was performed to compare log10(CFU+1)/mL between Groups C and D. The results showed that sodium hypochlorite (0.25% and 0.5%) completely eliminated all detectable microorganisms. The castor oil solution eliminatedB. subtilisand reduced counts for other strains. Differences between C and D were significant (p < 0.05) for all species except for E. faecalis. Both sodium hypochlorite solutions (0.25% and 0.5%) were effective in eliminating all microorganisms evaluated, and may be useful as cleaning solutions for complete dentures. The castor oil solution provided moderate efficacy and performed differently on the tested species, with the strongest effect on B. subtilis and with non-significant action on E. faecalis.

Este estudo avaliou a atividade antimicrobiana de um dentifrício experimental a base de Ricinus communis para higiene de prótese contra as seguintes cepas padrão: Staphylococcus aureus, Escherichia coli, Streptococcus mutans, Enterococcus faecalis, Candida albicans e Candida glabrata. O ensaio de concentração inibitória mínima foi realizado com R. communis em óleo puro a 2,5 % . Apenas a E. coli não foi inibida por R. communis, no entanto, a concentração mínima (0,0781%) foi eficaz contra os outros microrganismos. A partir destes resultados foram determinadas as concentrações dos dentifrícios experimentais: 1, 2, 5 e 10 %, as quais foram avaliadas pelo teste de difusão em Agar. Os dentifrícios comerciais Colgate, Trihydral e Corega Brite foram testados para fins comparativos. O diâmetro dos halos de inibição do crescimento bacteriano, em torno dos poços, foi medido (em mm) com uma régua sob uma luz refletida. Os dados foram analisados estatisticamente por meio de análise de variância e teste post-hoc de Tukey (α=0,05). Nem os dentifrícios comerciais nem os experimentais foram eficazes contra E. coli. Os dentifrícios experimentais contendo R. communis a 2 , 5 e 10 % apresentaram ação contra S. mutans, S. aureaus e E. faecallis. Os dentifrícios experimentais não mostraram atividade antimicrobiana contra Candida spp e E. coli em nenhuma das concentrações testadas . O Trihydral foi o mais eficaz. Comparando os dentifrícios experimentais, o produto com 10% de R. communis produziu os maiores halos de inibição do crescimento microbiano e apresentou atividade antimicrobiana.

This study evaluated the antimicrobial activity of a Ricinus communis-based experimental dentifrice for denture hygiene against the following standard strains: Staphylococcus aureus, Escherichia coli, Streptococcus mutans, Enterococcus faecalis, Candida albicans and Candida glabrata. The minimum inhibitory concentration (MIC) assay was performed with R. communis in pure oil at 2.5%. Only E. coli was not inhibited by R. communis, but the MIC (0.0781%) was effective against the other microorganisms. From these results it was determined the R. communis concentrations for experimental dentifrices, 1, 2, 5 and 10%, which were evaluated by the test-well diffusion in agar. The commercial dentifrices Colgate, Trihydral and Corega Brite were tested for comparative purposes. The diameter of the zones of bacterial growth inhibition produced around the wells was measured (in mm) with a rule under reflected light. Data were analyzed statistically by analysis of variance and Tukey's post-hoc test (α=0.05). Neither the commercial nor the experimental dentifrices were effective against E. coli. The experimental dentifrices containing R. communis at 2, 5 and 10% presented action against S. mutans, S. aureaus and E. faecallis. The experimental dentifrices showed no antimicrobial activity against Candida spp. and E. coli in any of the tested concentrations. Trihydral was the most effective. Comparing the experimental dentifrices, the product with 10% R. communis produced the largest zones of bacterial growth inhibition and had similar antimicrobial activity to the commercial dentifrices, except against S. aureus.

A higiene de próteses totais é essencial, uma vez que o biofilme da prótese está envolvido com infecções orais e doenças sistêmicas. Apesar de existirem agentes químicos disponíveis no mercado, nenhum deles apresenta propriedades ideais e pesquisas com esses produtos ainda são necessárias. O objetivo neste estudo foi avaliar a eficácia de uma solução de mamona (Ricinus communis) para a remoção do biofilme de prótese total, comparada com dois produtos comerciais (NaOCl e peróxido alcalino). Cinqüenta usuários de próteses totais maxilares foram instruídos a escovar as próteses após as refeições e imergi-las uma vez por dia nas seguintes soluções: soro fisiológico (20 min), Peróxido alcalino Polident (3 min), NaOCl a 1% (20 min) e solução de mamona (20 min). Os participantes utilizaram cada solução por um período de sete dias consecutivos, de acordo com uma sequência aleatória. Após cada período, as superfícies internas das próteses totais superiores foram coradas com solução evidenciadora (vermelho neutro a 1%), fotografadas e o biofilme evidenciado foi quantificado com software. A influência dos tratamentos sobre os resultados foi verificada pelo teste de Friedman (α=0,05). Houve diferença significativa entre as soluções (Fr=51,67;p<0,001). O soro fisiológico e o NaOCl foram significativamente diferentes (mediana: 2,0% e 0,0%), enquanto o Polident e a solução de mamona apresentaram resultados intermediários (mediana: 1,0% e 1,5%, respectivamente). Concluiu-se que a solução de mamona avaliada é comparável ao peróxido alcalino, em termos de remoção do biofilme de prótese total.

Denture hygiene is essential because denture biofilm is involved in oral infections and systemic diseases. Although there are chemical agents available on the market, none of them have ideal properties and research on such products is still necessary. The aim of this study was to evaluate the efficacy of a castor bean (Ricinus communis)-based solution for removing denture biofilm, compared to two traditional products (sodium hypochlorite and alkaline peroxide). Fifty maxillary complete denture wearers were instructed to brush their dentures after meals and to immerse their dentures once a day in the following solutions: Saline (20 min; control), Polident alkaline peroxide (3 min), NaOCl (20 min) and 2% castor oil solution (20 min). Participants used each solution for a period of 7 consecutive days, according to a random sequence. After each period, the internal surfaces of maxillary complete dentures were stained with a disclosing solution (1% neutral red), photographed and the disclosed biofilm was quantified with the aid of specific software. The influence of treatments on results was verified by the Friedman test (α=0.05). Tested solutions presented significant difference (Fr=51.67; p<0.001). Saline and NaOCl were significantly different (median: 2.0% and 0.0%) whereas Polident and castor oil presented intermediate results (median: 1.0% and 1.5%, respectively). It can be concluded that the castor oil solution tested in this study was comparable to alkaline peroxide in terms of efficiency in denture biofilm removal.

This study evaluated the effect of cleanser solutions on the color of heat-polymerized acrylic resin (HPAR) and on the brightness of dental alloys with 180 immersion trials. Disk-shaped specimens were made with I) commercially pure titanium, II) nickel-chromium-molybdenum-titanium, III) nickel-chromium molybdenum, and IV) nickel-chromium-molybdenum beryllium. Each cast disk was invested in the flasks, incorporating the metal disk into the HPAR. The specimens (n = 5) were then immersed in solutions containing: 0.05% sodium hypochlorite, 0.12% chlorhexidine digluconate, 0.500 mg cetylpyridinium chloride, a citric acid tablet, one of two different sodium perborate/enzyme tablets, and water. The color measurements (∆E) of the HPAR were determined by a colorimeter in accordance with the National Bureau of Standards. The surface brightness of the metal was visually examined for the presence of tarnish. The results (ANOVA; Tukey test-α = 0.05) show that there was a significant difference between the groups (p < 0.001) but not among the solutions (p = 0.273). The highest mean was obtained for group III (5.06), followed by group II (2.14). The lowest averages were obtained for groups I (1.33) and IV (1.35). The color changes in groups I, II and IV were slight but noticeable, and the color change was considerable for group III. The visual analysis showed that 0.05% sodium hypochlorite caused metallic brightness changes in groups II and IV. It can be concluded that the agents had the same effect on the color of the resin and that the metallic alloys are not resistant to the action of 0.05% sodium hypochlorite.

Resumo O estudo avaliou a alteração de cor, rugosidade de superfície e força de flexão de espécimes de resina acrílica após imersão em peróxido alcalino e hipoclorito alcalino, simulando um ano e meio de uso seguindo a imersão noturna. Sessenta espécimes circulares (16 X 4 mm) e oitenta retangulares (65 X 10 X 3,3 mm) de resina acrílica termopolimerizável (Lucitone 550) foram distribuídos em 4 grupos (n=20): C1: sem imersão, C2: 8 h de imersão em água destilada; PA: 8 h de imersão em pastilhas efervescentes de peróxido alcalino; HS: 8 h de imersão em hipoclorito de sódio a 0,5%. As propriedades foram avaliadas antes e após as imersões. Os dados de alteração de cor também foram calculados de acordo com o National Bureau of Standards (NBS). Os dados foram analisados estatisticamente pelo teste ANOVA e Tukey HSD (α=0,05). O PA (2,34 ± 0,41) causou alteração de cor significativamente maior que C2 (0,39 ± 0,30) e SH (1,73 ± 0,2). A média ΔE foi classificada como indicial para C2 (0,36 ± 0,29) e perceptível para PA (2,12 ± 0,39) e HS (1,59 ± 0,48). HS (0,0195 ± 0,0150) causou significantemente maior ΔRa (p=0) do que os demais (C2: 0,0005 ± 0,0115 e PA: 0,0005 ± 0,0157). Não houve diferença estatisticamente significante (p=0,063) entre as soluções para a resistência à flexão (C1: 105,43 ± 14,93; C2: 100,30 ± 12,43, PA: 97,61 ± 11,09, HS: 95,23 ± 10,18). A imersão noturna em soluções higienizadores de próteses simulando um ano e meio de uso não alterou a resistência à flexão da resina acrílica, porém causou alterações perceptíveis de cor, sendo maiores com o peróxido alcalino. O hipoclorito de sódio a 0,5% causou aumento da rugosidade de superfície.

This study evaluated color stability, surface roughness and flexural strength of acrylic resin specimens after immersion in alkaline peroxide and alkaline hypochlorite, simulating a period of one and a half year of use of overnight immersion. Sixty disc-shaped (16X4 mm) and 80 rectangular specimens (65X10X3.3 mm) were prepared from heat-polymerized acrylic resin (Lucitone 550) and distributed into 4 groups (n=20): C1: without immersion, C2: 8 h immersion in distilled water; AP: 8 h immersion in alkaline peroxide effervescent tablet; SH: 8 h immersion in 0.5% NaOCl solution. Properties were evaluated at baseline and after the immersion. Color data were also calculated according the National Bureau of Standards (NBS). Results were analyzed statistically by ANOVA and Tukey's HSD test (α=0.05). AP (2.34 ± 0.41) caused color alteration significantly higher than C2 (0.39 ± 0.30) and SH (1.73 ± 0.52). The mean ΔE values were classified as indicial for C2 (0.36 ± 0.29) and noticeable for AP (2.12 ± 0.39) and SH (1.59 ± 0.48). SH (0.0195 ± 0.0150) caused significantly higher ΔRa (p=0.000) than the C2 (0.0005 ± 0.0115) and PA (0.0005 ± 0.0157) groups. There was no statistically significant difference (p=0.063) among the solutions for flexural strength (C1: 105.43 ± 14.93, C2: 100.30 ± 12.43, PA: 97.61 ± 11.09, SH: 95.23 ± 10.18). In conclusion, overnight immersion in denture cleansing solutions simulating a year and a half of use did not alter the flexural strength of acrylic resin but caused noticeable color alterations, higher for alkaline peroxide. The 0.5% NaOCl solution caused increase in surface roughness.

Este estudo comparou os níveis de biofilme em próteses totais maxilares e mandibulares, e analisou o número de unidades formadoras de colônias de leveduras, após o uso de agentes auxiliares da escovação e saliva artificial. Vinte e três usuários de próteses totais com hipossalivação e xerostomia foram orientados a escovar as dentaduras 3 vezes ao dia durante 3 semanas com os seguintes produtos: Corega Brite (dentifrício para prótese), sabonete líquido neutro, Corega Brite associado com o uso do Oral Balance (saliva artificial) ou água de torneira. Para a quantificação do biofilme, as superfícies internas das próteses totais foram evidenciadas, fotografadas e o biofilme quantificado com o auxílio de um software. Para a análise microbiológica, o biofilme foi removido por escovação, coletado, diluído, semeado em meio seletivo CHROMagar™ Candida e incubado a 37°C por 48 h. A análise de variância para dois fatores (p<0,05%) mostrou que as próteses mandibulares apresentaram uma média de porcentagem de biofilme (µ=26,90±21,10) maior que as maxilares (µ=18±15). O teste complementar de Tukey (0,46; p<5%) mostrou que a escovação com Corega Brite e Oral Balance (µ=15,87 ± 18,47) foi mais efetiva que o dentifrício (µ=19,47 ± 17,24), sabonete neutro (µ=23,90 ± 18,63), ou água de torneira (controle; µ=32,50 ± 20,68). Em relação á análise microbiológica, o teste de Qui-Quadrado não indicou diferença entre os produtos de higiene, para ambas as próteses. As espécies de leveduras mais comumente isoladas foram C. albicans, C. tropicalis e C. glabrata. Em conclusão, as próteses mandibulares apresentaram mais biofilme do que as maxilares. Além disso, a escovação das próteses com o Corega Brite associado ao uso do Oralbalance foi o método mais efetivo na redução dos níveis de biofilme, entretanto o uso dos produtos não demonstrou diferença no número de ufc de leveduras.

This study compared the levels of biofilm in maxillary and mandibular complete dentures and evaluated the number of colony-forming units (cfu) of yeasts, after using auxiliary brushing agents and artificial saliva. Twenty-three denture wearers with hyposalivation and xerostomia were instructed to brush the dentures 3 times a day during 3 weeks with the following products: Corega Brite denture dentifrice, neutral liquid soap, Corega Brite combined with Oral Balance (artificial saliva) or tap water. For biofilm quantification, the internal surfaces of the dentures were disclosed, photographed and measured using a software. For microbiological analysis, the biofilm was scrapped off, and the harvested material was diluted, sown in CHROMagar™ Candida and incubated at 37°C for 48 h. Data were analyzed statistically by two-way ANOVA and Tukey's test (α=0.05). Mandibular dentures presented a mean biofilm percentage (µ=26.90 ± 21.10) significantly greater than the maxillary ones (µ=18.0 ± 15.0) (p<0.05). Brushing using Corega Brite combined with Oral Balance (µ=15.87 ± 18.47) was more effective (p<0.05) than using the denture dentifrice (µ=19.47 ± 17.24), neutral soap (µ=23.90 ± 18.63) or tap water (control; µ=32.50 ± 20.68). For the microbiological analysis, the chi-square test did not indicate significant difference between the hygiene products for either type of denture. The more frequently isolated species of yeasts were C. albicans, C. tropicalis and C. glabrata. In conclusion, mandibular dentures had more biofilm formation than maxillary ones. Denture brushing with Corega Brite dentifrice combined with the use of Oral Balance was the most effective method for reduction of biofilm levels, but the use of products did not show difference in yeast cfu counts.

Este estudo analisou a perda de massa e rugosidade superficial causadas em corpos de prova de Plexiglass por dentifrícios convencionais (Sorriso, Colgate e Close Up) e específicos (Corega e Dentu Creme) para higienização de próteses totais. Os corpos de prova de Plexiglass foram distribuidos em 6 grupos (n=6), sendo um controle (água destilada - AD) e grupos experimentais. A escovação foi realizada em máquina de escovação com escovas macias e suspensões de dentifrícios ou AD, de acordo com os diferentes tempos de escovação (50, 100, 200 e 250 min -18.000, 36.000, 72.000 e 90.000 ciclos, respectivamente, calculados para corresponder a 1, 2, 4 e 5 anos de escovação regular). Os resultados de perda de massa e rugosidade superficial foram analisados por meio de ANOVA e teste de Tukey com 5% de significância. Em todos os tempos analisados, a AD teve efeito insignificante. Os dentifrícios diferiram significativamente da AD no período inicial. O dentifrício Corega causou maior perda de massa em todos os tempos estudados, seguido por Close Up. No tempo de 50 min, todos os dentifrícios causaram rugosidade superficial semelhante à AD. Nos outros tempos, Sorriso, Colgate e Corega causaram maior rugosidade superficial em relação à AD. Conclui-se que os dentifrícios específicos causaram maior perda de massa, porém menor rugosidade superficial que os dentifrícios convencionais.

This study analyzed the weight loss and surface roughness caused in Plexiglass specimens by conventional dentifrices (Sorriso, Colgate and Close Up) and specific dentifrices used for cleaning of dentures (Corega and Dentu Creme). Plexiglass specimens were divided into 6 groups (n=6) including: a control (distilled water - DW) and experimental groups. Brushing was performed in a toothbrushing machine with a soft brush and a dentifrice suspension and DW according to different brushing times (50, 100, 200 and 250 min -18,000, 36,000, 72,000 and 90,000 cycles, respectively, calculated to correspond to 1, 2, 4 and 5 years of regular brushing). The results of weight loss and surface roughness were analyzed by ANOVA and Tukey’s test at 5% significance level. In all tested times, the effect of DW was insignificant. Dentifrices differed significantly from DW in the initial period. Corega dentifrice caused greater mass loss in all studied times, followed by Close Up. Dentifrices resulted in a surface roughness similar to the DW at 50 min. In the other times, Sorriso, Colgate and Corega caused more surface roughness than DW. In conclusion, specific dentifrices caused larger mass loss and lower surface roughness as conventional dentifrice.