Aim To compare performance differences among dental surgeons in the public sector of the state of Minas Gerais regarding prenatal dental care before and after the establishment of Previne Brasil. Methods A cross-sectional study was conducted, gathering data through a structured questionnaire. The sample consisted of dental surgeons working in the public sector of municipalities in MG and utilizing the snowball effect. Data analysis was performed using the IBM-SPSS® version 29.0, employing Pearson’s Chi-square statistical test (P<0.05). Results Out of the 28 State Health System (SRS) present in MG. A total of 1696 responses were collected, but after applying the exclusion criteria, 1001 responses remained, specifically related to dental surgeons working in MG public sector. 78.6% comprised professionals who graduated before 2019, while 20.1% graduated after that year. The morning and the second or other gestational trimesters were the most common periods considered optimal for caring for pregnant women. A significant portion of the professionals had not undergone any specific courses or training on this topic, reporting an average satisfaction level of 5.52 regarding the accessibility of information during their education aimed at this audience. Conclusion Variances were observed in the care provided to pregnant women by dental surgeons working in the public sector of Minas Gerais, distinguishing those trained before and after the implementation of Previne Brasil. The debunking of myths and taboos stands as crucial for enhancing the acceptance of prenatal dentistry. Brasil crosssectional cross sectional conducted questionnaire effect IBMSPSS® IBMSPSS IBM SPSS® SPSS IBM-SPSS 290 29 0 29.0 Pearsons Pearson s Chisquare Chi square P<0.05. P005 P P<0.05 . 05 (P<0.05) 2 SRS (SRS 169 collected criteria 100 remained 786 78 6 78.6 2019 201 20 1 20.1 year topic 552 5 52 5.5 audience dentistry 29. P00 P<0.0 (P<0.05 16 10 7 78. 20. 55 5. P0 P<0. (P<0.0 P<0 (P<0. P< (P<0 (P< (P

Abstract Background Genetic polymorphisms have been shown to influence several physiological traits, including dental and craniofacial characteristics. Understanding the clinical relevance of genetic polymorphisms in dental practice is crucial to personalize treatment plans and improve treatment outcomes. Objective to evaluate the association between dental age and genetic polymorphisms in genes encoding estrogen receptors alpha and beta (ESR1 and ESR2, respectively) in a sample of Brazilian children. Methodology This retrospective cross-sectional study was performed with children undergoing orthodontic treatment. Patients with syndromes, congenital anomalies, craniofacial deformities, under hormonal or systemic treatment, and with a previous history of facial trauma were excluded. Panoramic radiographs were used to assess dental age according to the Demirjian, Goldstein, and Tanner method. A delta [dental age-chronological age (DA-CA)] was obtained, which shows whether the patient tends to have a normal, delayed (negative values), or advanced (positive values) dental age. DNA isolated from buccal cells was used to genotype four genetic polymorphisms: rs9340799 (A>G) and rs2234693 (C>T), located in ESR1; and rs1256049 (C>T) and rs4986938 (C>T), located in ESR2. A statistical analysis was performed and values of p<0.05 indicated statistical difference. Results A total of 79 patients were included, 44 (55.70%) girls and 35 (44.30%) boys. The Demirjian, Goldstein, and Tanner method, in general, overestimated patients’ age by 0.75 years. There was no difference in the delta of dental age between the sexes (p>0.05). Genetic polymorphisms in ESR1 and ESR2 were not associated with dental age (p>0.05). Conclusion The studied genetic polymorphisms in ESR1 and ESR2 were not associated with dental age in Brazilian children. traits characteristics outcomes ESR (ESR respectively crosssectional cross sectional syndromes anomalies deformities excluded Demirjian Goldstein method agechronological chronological DACA DA CA (DA-CA) obtained normal negative values, , positive rs rs934079 A>G AG G (A>G rs223469 C>T, CT C>T C T rs125604 (C>T rs498693 p005 p 0 05 p<0.0 7 included 4 55.70% 5570 55 70 (55.70% 3 44.30% 4430 30 (44.30% boys general 075 75 0.7 years p>0.05. p>0.05 . (p>0.05) (DA-CA rs93407 rs22346 rs12560 rs49869 p00 p<0. 55.70 557 5 (55.70 44.30 443 (44.30 07 0. p>0.0 (p>0.05 rs9340 rs2234 rs1256 rs4986 p0 p<0 55.7 (55.7 44.3 (44.3 p>0. (p>0.0 rs934 rs223 rs125 rs498 p< 55. (55. 44. (44. p>0 (p>0. rs93 rs22 rs12 rs49 (55 (44 p> (p>0 rs9 rs2 rs1 rs4 (5 (4 (p> ( (p

ABSTRACT Objective: To investigate the association between single nucleotide polymorphisms in the COX2 gene (rs689466 and rs5275) and local and systemic signs and symptoms of teething. Material and Methods: Forty-four pairs of mothers-babies/toddlers were included. Erupted primary teeth were evaluated during clinical examination. Local and systemic signs and symptoms of teething were obtained from mothers' reporting via anamnesis. Samples of buccal cells were retrieved for DNA genotyping using real-time PCR. The T-test, Chi-square test, logistic regression, and haplotype analyses were applied. Results: Almost all mothers (95.5%) reported at least one local or systemic sign and symptom of teething. The most common was increased salivation (79.5%), diarrhea (72.3 %), and fever (70.5 %). The mean number of signs and symptoms per child was higher in boys than girls (mean = 5.1; SD= 1.5; p=0.008). Sleep disturbance (p=0.03) and loss of appetite (p=0.05) were more reported in boys. The rs689466 and rs5275 were not associated with signs and symptoms of teething (p>0.05). Conclusion: The single nucleotide polymorphisms in the COX2 gene (rs689466 and rs5275) were not associated with local and systemic signs and symptoms of teething. Objective COX rs (rs68946 Methods Fortyfour Forty four mothersbabies/toddlers mothersbabiestoddlers babies/toddlers babies toddlers included examination anamnesis realtime real time PCR Ttest, Ttest T test T-test Chisquare Chi square regression applied Results 95.5% 955 95 5 (95.5% 79.5%, 795 79.5% , 79 (79.5%) 72.3 723 72 3 (72. %, % %) 70.5 705 70 (70. %. . 5.1 51 1 SD 1.5 15 p=0.008. p0008 p p=0.008 0 008 p=0.008) p=0.03 p003 03 (p=0.03 p=0.05 p005 05 (p=0.05 rs68946 rs527 p>0.05. p>0.05 (p>0.05) Conclusion (rs6894 mothersbabies babiestoddlers 95.5 9 (95.5 79.5 7 (79.5% 72. (72 70. (70 5. 1. p000 p=0.00 00 p=0.0 p00 (p=0.0 rs6894 rs52 p>0.0 (p>0.05 (rs689 95. (95. 79. (79.5 (7 p=0. p0 (p=0. rs689 rs5 p>0. (p>0.0 (rs68 (95 (79. ( p=0 (p=0 rs68 p>0 (p>0. (rs6 (9 (79 p= (p= rs6 p> (p>0 (rs (p (p>

RESUMO O objetivo do presente estudo foi investigar a associação entre polimorfismos de nucleotídeo único (SNPs) em genes que codificam receptores de estrógeno (ESR1 e ESR2, respectivamente) e o retardo na emergência dentária (DTE). Este estudo transversal foi composto por crianças biológicas não relacionadas de ambos os sexos, com idades entre 11 e 13 anos. O DTE foi definido pela presença do dente decíduo na cavidade bucal após seu tempo e também, quando as crianças apresentaram pelo menos um dente permanente com atraso. O DNA genômico foi usado para avaliar os SNPs em ESR1 (rs9340799 e rs2234693) e ESR2 (rs1256049 e rs4986938) usando PCR em tempo real. Foram realizados testes Qui-quadrado ou exato de Fisher e Regressão Logística ajustados por idade e sexo. A interação SNP-SNP foi acessada pela análise de redução de dimensionalidade multifatorial (MDR), também ajustada por sexo e idade. O alfa de 5% foi estabelecido. Entre 537 crianças incluídas, 296 (55%) estavam no grupo “DTE” e 241 (45%) estavam no grupo “Controle”. A idade e o sexo não foram estatisticamente diferentes entre os grupos (p> 0,05). A distribuição de genótipos dos SNPs rs9340799, rs2234693, rs1256049 e rs4986938 não foi associada ao DTE (p> 0,05). Os modelos eleitos pelo MDR também não foram estatisticamente significativos. Conclusões: Os SNPs estudados na ESR1 e ESR2 não foram associados ao DTE na dentição permanente.

Abstract The purpose of the study was to investigate the association between single nucleotide polymorphisms (SNPs) in genes encoding estrogen receptors (ESR1 and ESR2, respectively) and delayed tooth emergence (DTE). This cross-sectional study was composed of biological unrelated children of both sexes, age ranging from 11 to 13 years old. DTE was defined when the successor primary tooth was still present in the oral cavity after its exfoliation time or the absence of the permanent tooth emergence into the oral cavity. Children were diagnosed with DTE when they had at least one delayed permanent tooth, according to age of exfoliation of each tooth proposed by The American Dental Association. Genomic DNA from saliva was used to evaluate the SNPs in ESR1 (rs9340799 and rs2234693) and ESR2 (rs1256049 and rs4986938) using Real-Time PCR. Chi-square or Fisher exact tests and Logistic Regression adjusted by age and gender were performed. SNP-SNP interaction was accessed by multifactor dimensionality reduction (MDR) analysis also adjusted by gender and age. The established alpha of this study was 5%. Among 537 included children, 296 (55%) were in the “DTE” group and the 241 (45%) were in the “Control” group. Age and gender were not statistically different among the groups (p>0.05). Genotype distribution of the SNPs rs9340799, rs2234693, rs1256049 and rs4986938 were not associated with DTE (p> 0.05). The models elected by MDR were not statistically significant either. Conclusions: The studied SNPs in ESR1 and ESR2 were not associated with permanent DTE.