For biotechnological purposes, protein expression refers to the directed synthesis of large amounts of desired proteins. The aim of the present work was to produce reverse transcriptase Moloney murine Leukaemia Virus retro-transcriptase and Taq DNA polymerase, as bioactive products. In the present paper, we report the preparation of recombinant enzymes, expressed in E. coli strains. The enzymes produced exhibited quite good activity, compared with commercial enzymes, allowing us to replace the last ones for several lab applications. We are reporting changes and modifications to standard protocols described. The standard protocols were modified, i.e. for the purification step of Taq, a temperature dependent procedure was designed. The enzymes produced were used in different applications, such as PCR, RT-PCR, PCR Multiplex and RAPDs molecular markers.

O presente trabalho tem como objetivo estudar o sistema de franquia como difusor de inovações tecnológicas e organizacionais no processo produtivo de uma empresa de construção civil, no subsetor de edificações. Para atingir este objetivo, realizou-se um estudo de caso na Construtora Akyo Ltda., franqueada da Construtora Rossi Residencial com o Plano 100 de Habitação. Analisaram-se as inovações organizacionais e tecnológicas introduzidas pela franquia, tomando como base o processo tradicional executado pela empresa anteriormente; e o impacto causado na produtividade, através da comparação de índices obtidos com a franquia e índices da construção tradicional.

The present work is intended to analyze the franchise system´s role on the promotion and diffusion of organizational and technological innovations in the productive process of a building firm (contractor). In order to do that, a case study was conducted in Akyo's "Plano 100", where were analyzed the technological and organizational innovations introduced by the franchise. The traditional process previously executed by the company was used for productivity comparison purposes.