Abstract Traditional complementary and alternative therapies are the main targets for pharmaceutical agents. Natural biomolecules from medicinal plants are rich sources of drugs targeting the amelioration or prevention of different diseases with low adverse effects. Saudi Arabia's normal flora is traditionally used as an anti-inflammatory, hypoglycemic, hypolipidemic, hypotension, and GIT disturbance. In the current study, we used the GC/MS technique for the identification of the chemical composition of many medicinal herbs as Artemisia monosperma, Mentha longifolia, and Rumex nervosus, using methanol and chloroform extracts. Data obtained showed that terpenoids are the most active compound, identified as eucalyptol, menthol, 1,4-benzenediol, cyclobuta (1,2:3,4) dicyclopentene, eicosane, phytol, and heptacosane. The data endorse the conventional application of these plants for treating many diseases. The 1,8-cineole is present in various mouthwash brands, and it has been observed to exhibit anti-plaque and demonstrated antibacterial properties. Eucalyptol also has an anti-inflammatory effect that was assessed by measuring its relative potency compared to prednisolone in individuals suffering from severe asthma. It was concluded that these plant extracts are a promising new source of medications due to the presence of such phytochemicals and bioactive compounds. Further investigation is required to clarify their precise mechanisms of action in different disorders. agents effects Arabias Arabia s antiinflammatory, antiinflammatory anti inflammatory, inflammatory hypoglycemic hypolipidemic hypotension disturbance study GCMS GC MS monosperma longifolia nervosus compound eucalyptol menthol 1,4benzenediol, 14benzenediol benzenediol 1,4 benzenediol, 1 4 1,4-benzenediol 1,23,4 1234 1,2 3,4 2 3 (1,2:3,4 dicyclopentene eicosane phytol heptacosane 1,8cineole 18cineole cineole 1,8 8 brands antiplaque plaque properties asthma compounds disorders 4benzenediol 1,4benzenediol 14 1, 23 1,23, 123 12 34 3, (1,2:3, 8cineole 18 1,23 (1,2:3 (1,2: (1,2 (1, (1 (

Resumo O câncer colorretal (CCR) é um dos cânceres mais comuns, levando a comorbidades e mortalidade em todo o mundo. O racional do presente estudo foi avaliar a combinação de galato de epigalocatequina e quercetina como um agente antitumoral potente como agente de comentário para protocolo terapêutico. O presente estudo investigou o efeito de galato de epigalocatequina (EGCG) (150 mg) e quercetina (200 mg) em diferentes proporções na proliferação e indução de apoptose em células de câncer de cólon humano (HCT-116). O crescimento celular, colonogênico, anexina V, além do ciclo celular foram detectados em resposta a fitomoléculas. Os dados obtidos mostraram que a formação de colônias foi inibida significativamente no CRC a partir da concentração mais baixa testada de 10 µg/mL, resultando em nenhuma colônia conforme visualizado por um microscópio de contraste de fase. Os dados mostraram uma elevação significativa na anexina V a 100 µg/mL de EGCG (25,85%) e 150 µg/mL de quercetina (48,35%). Além disso, a análise do ciclo celular mostrou que essa combinação causou parada do ciclo celular na fase G1 na concentração de 100 µg/mL (72,7%) e 150 µg/mL (75,25%). O efeito combinado da epigalocatequina galato e quercetina exerce atividade antiproliferativa contra o CCR, é promissor como agente quimioterápico alternativo convencional.

Abstract Colorectal cancer (CRC) is one of the most common cancers leading to comorbidities and mortalities globally. The rational of current study was to evaluate the combined epigallocatechin gallate and quercetin as a potent antitumor agent as commentary agent for therapeutic protocol. The present study investigated the effect of epigallocatechin Gallate (EGCG) (150mg) and quercetin (200mg) at different proportions on proliferation and induction of apoptosis in human colon cancer cells (HCT-116). Cell growth, colonogenic, Annexin V in addition cell cycle were detected in response to phytomolecules. Data obtained showed that, the colony formation was inhibited significantly in CRC starting from the lowest concentration tested of 10 µg/mL resulting in no colonies as visualized by a phase-contrast microscope. Data showed a significant elevation in the annexin V at 100 µg/mL EGCG(25.85%) and 150 µg/mL quercetin (48.35%). Moreover, cell cycle analysis showed that this combination caused cell cycle arrest at the G1 phase at concentration of 100 µg/mL (72.7%) and 150 µg/mL (75.25%). The combined effect of epigallocatechin Gallate and quercetin exert antiproliferative activity against CRC, it is promising in alternative conventional chemotherapeutic agent.

Cinnamon is used to flavor most foods in Arabian countries. The aim of this study was to evaluate the medicinal importance, reflecting an important trend in research. The hepatoprotective activity of aqueous and ethanolic extracts of cinnamon was investigated against carbon tetrachloride (CC1(4)) induced lipid peroxidation and hepatic injury in rats. The elevated serum AST and ALT enzymatic activities induced by CC1(4) were significantly restored to near normal by oral administration of 200 mg/kg of either extracts once daily for 7 days, as compared to untreated rats. There was a significant elevation in the level of liver malondialdhyde (MDA), while the activities of antioxidant enzymes superoxide dismutase and catalase (SOD and CAT) were significantly decreased in CC1(4) intoxicated rats. The results obtained indicated that ethanolic extract has more potent hepatoprotective action than water extract against CC1(4) by lowering the MDA level and elevating antioxidants enzymes activities (SOD and CAT). The possible mechanism of this activity may be free radical-scavenging polyphenol compounds. The hepatoprotective properties were documented by the histopathological data obtained. Consequently, this extract can be used as a therapeutic regime in treatment of some hepatic disorders without any side effects. Further study will be done for separation and identification of active components and for testing antitumor activity.