ABSTRACT Purpose: To evaluate using a biocellulose-based hydrogel as an adjuvant in the healing process of arterial ulcers. Methods: A prospective single group quasi-experimental study was carried out with chronic lower limb arterial ulcer patients. These patients received biocellulose-based hydrogel dressings and outpatient guidance on dressing and periodic reassessments. The primary outcomes were the ulcer-healing rate and product safety, which were assessed by ulcer area measured in photographic records of pre-treatment and posttreatment after 7, 30, and 60 days. Secondary outcomes were related to clinical assessment by the quality-of-life scores (SF-36 and EQ-5D) and pain, evaluated by the visual analogue scale (VAS). Results: Seventeen participants were included, and one of them was excluded. Six patients (37%) had complete wound healing, and all patients had a significant reduction in the ulcer area during follow-up (233.6mm2 versus 2.7mm2) and reduction on the score PUSH 3.0 (p < 0.0001). The analysis of the SF-36 and EQ-5D questionnaires showed a statistically significant improvement in almost all parameters analyzed and with a reduction of pain assessed by the VAS. Conclusions: The biocellulose-based hydrogel was safe and showed a good perspective to promoting the necessary conditions to facilitate partial or complete healing of chronic arterial ulcers within a 60-day follow-up. Quality of life and pain were positively affected by the treatment. Purpose biocellulosebased biocellulose based Methods quasiexperimental quasi experimental reassessments ulcerhealing safety pretreatment pre treatment 7 30 6 days qualityoflife quality SF36 SF 36 (SF-3 EQ5D EQD EQ 5D D VAS . (VAS) Results included excluded 37% 37 (37% followup follow up 233.6mm2 2336mm2 mm 233 6mm2 (233.6mm 2.7mm2 27mm2 2 7mm2 3 0 3. p 0.0001. 00001 0.0001 0001 0.0001) SF-3 Conclusions 60day day followup. up. SF3 (SF- (VAS (37 233.6mm 2336mm 23 6mm 2.7mm 27mm 7mm 0000 0.000 000 SF- (SF (3 0.00 00 ( 0.0 0.