To expand the variety of density functionalized compounds evaluated against severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2), we decided to prepare new acetylated and disubstituted 3-hydroxy bis-oxindoles and isoindigos coupling compounds using known protocols. The corresponding isatin derivatives were synthesized by ZrCl4/EtOH/reflux or HCl/AcOH/reflux coupling conditions using oxindole and functionalized isatins, furnishing new 3-hydroxy bis-oxindoles, which were dehydrated into new isoindigos. A total of 27 compounds bearing halogen, nitro and/or hydroxy groups on the isatin moiety at the 3-, 5- and 7-positions, were prepared, including 5 new 3-hydroxy bis-oxindoles and 3 new halogenated isoindigos prepared according to adapted procedures described in the literature. This library of nitrogen-isatin derivatives was evaluated against SARS-CoV-2 using a phenotypic screening assay. In this investigation, isatin derivatives 3d, 3e, 3h and 3i showed antiviral activity when tested at a single concentration. Compound 3e showed antiviral activity against SARS-CoV-2 in the concentration-response assay; however, it showed cellular toxicity in Vero cells. The present study identified substituted isatins as a promising new starting point for the development of anti-SARS-CoV-2 agents.

Abstract Objective To investigate the clinicopathological significance and prognosis of the expression of the anterior gradient 3 (AGR3) protein in women with breast cancer. Data Sources The PubMed, CINAHL, EMBASE, Scopus, and Web of Science databases were searched for studies published in English and without restrictions regarding the year of publication. The search terms were: breast cancer AND anterior gradient 3 OR AGR3 expression. Study Selection We included observational or interventional studies, studies on AGR3 protein expression by immunohistochemistry, and studies on invasive breast cancer. Case reports, studies with animals, and reviews were excluded. In total, 4 studies were included, containing 713 cases of breast cancer. Data Collection Data were extracted on clinicopathological characteristics and survival. A meta-analysis of the prevalence of AGR3 expression was performed according to the clinicopathological characteristics, hazard ratios (HRs), and overall survival and disease-free survival. Data Synthesis The expression of AGR3 was found in 62% of the cases, and it was associated with histological grade II, positivity of estrogen and progesterone receptors, low expression of ki67, recurrence or distant metastasis, and lumen subtypes. In patients with low and intermediate histological grades, AGR3 expression was associated with worse overall survival (HR: 2.39; 95% confidence interval [95%CI]: 0.628–4.159; p= 0.008) and worse disease-free survival (HR: 3.856; 95%CI: 1.026–6.686; p= 0.008). Conclusion The AGR3 protein may be a biomarker for the early detection of breast cancer and predict prognosis in luminal subtypes. In addition, in patients with low and intermediate histological grades, AGR3 protein expression may indicate an unfavorable prognosis in relation to survival.

Resumo Objetivo Investigar o significado clinicopatológico e prognóstico da expressão da proteína anterior gradient 3 (AGR3) em mulheres com câncer de mama. Fontes de Dados Utilizamos as bases de dados PubMed, CINAHL, EMBASE, Scopus e Web of Science para pesquisar estudos em inglês, sem restrições quanto ao ano de publicação. Os termos buscados foram: breast cancer AND anterior gradient 3 OR AGR3 expression. Seleção dos Estudos Foram incluídos estudos observacionais ou intervencionais, estudos sobre a expressão da proteína AGR3 por imuno-histoquímica, e estudos sobre câncer de mama invasivo. Excluíram-se relatos de casos, estudos com animais e revisões. Quatro estudos foram selecionados, que continham 713 casos de câncer de mama. Coleta de Dados Foram extraídos dados relativos a características clinicopatológicas e sobrevida. A metanálise da prevalência da expressão de AGR3 foi realizada conforme as características clinicopatológicas, razões de risco (RRs) e sobrevida global (SG) e sobrevida livre de doença (SLD). Síntese dos Dados Encontrou-se expressão de AGR3 em 62% dos casos, que se associou com grau histológico II, positividade de receptores de estrogênio e progesterona, baixa expressão de ki67, recorrência ou metástase à distância e subtipos luminais. Em pacientes com graus histológicos baixo e intermediário, a expressão de AGR3 conferiu pior SG (RR: 2,39; intervalo de confiança de 95% [IC95%]: 0,628–4,159; p= 0,008) e pior SLD (RR: 3,856; IC95%: 1,026–6,686; p= 0,008). Conclusão A AGR3 pode ser um biomarcador para a detecção precoce do câncer de mama e predizer o prognóstico em subtipos luminais. Em graus histológicos baixo e intermediário, a expressão da proteína AGR3 pode indicar um prognóstico desfavorável em relação à sobrevida.

Chagas disease is a neglected tropical disease caused by the protozoan parasite Trypanosoma cruzi. There is an urgent need for safe, effective, and accessible new treatments since the currently approved drugs have serious limitations. Drug development for Chagas disease has historically been hampered by the complexity of the disease, critical knowledge gaps, and lack of coordinated R&D efforts. This review covers some of the translational challenges associated with the progression of new chemical entities from preclinical to clinical phases of development, and discusses how recent technological advances might allow the research community to answer key questions relevant to the disease and to overcome hurdles in R&D for Chagas disease. cruzi safe effective limitations gaps RD R D efforts

Resumo O objetivo do trabalho foi avaliar o efeito do hidrogel no plantio de mudas clonais de Eucalyptus urograndis em vasos, relacionados com diferentes manejos hídricos, buscando fornecer informações sobre a eficiência do uso do hidrogel. O experimento foi conduzido em viveiro florestal, esquema fatorial, em que o fator “A” se referiu à ausência e à presença de hidrogel e o fator “B” ao manejo hídrico (irrigação somente no plantio, 8 dias, 4 dias e diariamente). Foram realizadas avaliações da sintomatologia do estresse hídrico, características morfológicas, teores de clorofila, teor de água no solo, trocas gasosas, incremento em diâmetro e altura e biomassa seca. O hidrogel proporcionou um aumento no teor de água no solo e um atraso nos sintomas de estresse hídrico, aumentando a taxa de sobrevivência. O estresse hídrico foi um fator limitante para a abertura estomática, contribuindo para a queda na assimilação de carbono pela fotossíntese.

Abstract The objective of this study was to evaluate the effect of the hydrogel on the planting of clonal Eucalyptus urograndis seedlings in pots, related to different water management, to provide information about the hydrogel use efficiency. The experiment was carried out in a forest nursery in a factorial scheme, as factor “A” referred to the absence and presence of hydrogel and factor “B” to water management (0 - irrigation only at planting, 1 - daily, 4 - every 4 days, and 8 - every 8 days). Evaluations of the water stress symptomatology, morphological characteristics, chlorophyll content, soil water content, gas exchange, increase in diameter and height, and dry biomass were performed. The hydrogel provided an increase in water content in the soil and a delay in the symptoms of water stress, increasing the survival rate. Water stress was a limiting factor for stomatal opening, contributing to the decrease in carbon assimilation by photosynthesis.

Resumo Sequoia sempervirens (sequoia) é uma espécie conhecida pela qualidade superior de sua madeira, sendo a produção de mudas por miniestaquia uma alternativa viável. O objetivo do trabalho foi avaliar a posição de coleta, presença ou ausência de folhas aciculares em miniestacas e a produção de mudas cultivadas em diferentes substratos e utilização de AIB. Foram utilizadas miniestacas de diferentes clones, oriundas de minijardim clonal. Para o padrão de miniestacas utilizou-se a porção lignificada, com média lignificação e pouca lignificação, na presença ou ausência de folhas aciculares, avaliando-se um clone. Em relação aos substratos, observou-se o comportamento de três clones e quatro substratos de marcas comerciais. Para o emprego de regulador de crescimento utilizou-se um clone e diferentes concentrações de AIB (0,0 a 5000 mg L-1). As miniestacas foram colocadas para enraizar em estufim e avaliadas aos 90 dias. Todos os experimentos foram instalados em delineamento inteiramente casualizado, com posterior avaliação da porcentagem de sobrevivência; porcentagem de miniestacas com calos; porcentagem de miniestacas enraizadas e número de raízes emitidas por miniestaca enraizada. Miniestacas pouco lignificadas (ápice) com folhas aciculares apresentaram os melhores resultados para sobrevivência (96%) e enraizamento (94%); miniestacas muito lignificadas sem folhas aciculares obtiveram baixo enraizamento (10%). Houve considerável variação de enraizamento entre os substratos (66 a 100%) e o número de raízes foi mais influenciado pelo clone utilizado. Os melhores resultados foram observados naqueles com componentes mais orgânicos. O emprego de AIB na miniestaquia não foi significativo para nenhuma das variáveis analisadas. Miniestacas coletadas em porções apicais e com presença de folhas aciculares mostraram-se mais indicadas para trabalhos com a espécie. Para a seleção do melhor substrato deve-se observar o efeito clonal, priorizando-se substratos com composição mais orgânica. A utilização de AIB não influenciou positivamente o enraizamento de miniestacas, não sendo recomendado o seu uso.

Abstract Sequoia sempervirens (sequoia) is a species known for the superior quality of its wood, making plants production by minicuttings a viable alternative. The objective of the work was to evaluate the position of collection, presence or absence of acicular leaves in minicuttings and production of plants grown on different substrates and use of IBA. Minicuttings from different clones, from a clonal minigarden, were used. For the minicuttings pattern, use a lignified portion, with medium lignification and little lignification, in the presence or absence of acicular leaves, available if a clone. Regarding substrates, the behavior of three clones and four substrates of commercial brands. For the growth regulator, was used a clone and different concentrations of IBA (0.0 to 5000 mg L-1). Minicuttings were placed to root in mini-tunnel and evaluated at 90 days. All experiments were installed in completely randomized design, with subsequent assessment of the percentage of survival; percentage of minicuttings with calluses; percentage of rooted minicuttings and number of roots emitted by rooted minicuttings. Little lignified minicuttings (apex) with acicular leaves, showed the best results for survival (96%) and rooting (94%); very lignified minicuttings without acicular leaves obtained low rooting (10%). There was considerable variation in rooting between substrates (66 to 100%) and the number of roots was more influenced by the clone used. The best results were observed in those with more organic components. The use of IBA in the minicutting was not significant for any of the variables analyzed. Minicuttings collected in apical portions and with the presence of acicular leaves are more suitable for work with a species. To select the best substrate, must be observe the clonal effect, prioritizing substrates with a more organic composition. The use of IBA did not positively influence or reduce minicuttings, it was not recommended or its use.

Abstract The emergence of the new corona virus (SARS-CoV-2) and the resulting COVID-19 pandemic requires fast development of novel prevention and therapeutic strategies. These rely on understanding the biology of the virus and its interaction with the host, and on agnostic phenotypic screening for compounds that prevent viral infection. In vitro screenings of compounds are usually performed in human or animal-derived tumor or immortalized cell lines due to their ease of culturing. However, these platforms may not represent the tissues affected by the disease in vivo, and therefore better models are needed to validate and expedite drug development, especially in face of the COVID-19 pandemic. In this scenario, human induced pluripotent stem cells (hiPSCs) are a powerful research tool due to their ability to generate normal differentiated cell types relevant for the disease. Here we discuss the different ways hiPSCs can contribute to COVID-19 related research, including modeling the disease in vitro and serving as a platform for drug screening.

Resumo: Este estudo objetivou apreender as representações sociais (RSs) de estudantes da Educação de Jovens e Adultos (EJA) acerca da escola, da própria modalidade “educação de jovens e adultos” e do futuro. Os resultados indicaram que a RS da escola é ancorada à aquisição de conhecimento e também à socialização. A EJA é tida como uma oportunidade de concluir os estudos, de obter conhecimento e uma formação. O futuro por vezes é representado pela família, trabalho, possibilidade de emprego, casar e cuidar dos filhos.

Abstract: This study aimed at apprehending the social representations (RSs) of students of Youth and Adult Education (EJA) about the school, the modality “youth and adult education” itself, and the future. The results indicated that the RS of the school is anchored to the acquisition of knowledge and the socialization. The EJA is an opportunity to complete the studies, obtain knowledge, and training. The future, by several times, is represented by family, work, the possibility of employment, marriage, and care of children.

Resumen: Este estudio objetivó aprehender las representaciones sociales (RSs) de estudiantes de la Educación de Jóvenes y Adultos (EJA) acerca de la escuela, de la propia modalidad “educación de jóvenes y adultos” y del futuro. Los resultados indicaron que la RS de la escuela está anclada a la adquisición de conocimiento y también a la socialización. La EJA se considera como una oportunidad de concluir los estudios, de obtener conocimiento y una formación. El futuro, por su vez, es representado por la familia, trabajo, posibilidad de empleo, casarse y cuidar de los hijos.

Resumo A adição de polímeros hidroretentores visa aumentar a capacidade de retenção de água no solo, propiciando maior taxa de sobrevivência das mudas e permitindo o plantio em épocas distintas do ano. O objetivo do presente estudo foi avaliar o efeito do uso do hidrogel no plantio de mudas de Eucalyptus dunnii em vasos, relacionado com diferentes manejos hídricos, simulando condições de campo em solo do tipo Cambissolo Húmico. O experimento foi conduzido em delineamento inteiramente casualizado, com arranjo fatorial (hidrogel e frequência de irrigação). O fator hidrogel foi composto por presença ou ausência do polímero, e a frequência de irrigação por irrigação somente no plantio, a cada 8 dias, 4 dias e diariamente. Foram realizadas avaliações da sintomatologia do estresse hídrico, aspectos morfométricos, teor de água no solo e trocas gasosas. O hidrogel proporcionou aumento no teor de água no solo e o adiamento nos sintomas de estresse hídrico, principalmente quando a irrigação foi efetuada com menor frequência. A adição do polímero também proporcionou melhoria na qualidade das mudas, com maior acúmulo de massa seca. O estresse hídrico foi um fator limitante para a abertura estomática. Quanto menor a disponibilidade de água, menores foram os valores de fotossíntese, condutância estomática e transpiração. Em geral, os resultados obtidos confirmam que a adição de hidrogel no solo otimiza a disponibilidade de água, com possibilidade de redução da mortalidade no plantio e maximização do crescimento das plantas na fase inicial de estabelecimento.

Abstract The addition of water-repelling polymers aims to increase water retention capacity in the soil, providing higher seedling survival rate and allowing planting at different times of the year. The objective of the present work was to evaluate the effect of hydrogel use on planting Eucalyptus dunnii seedlings in pots, related to different water management, simulating field conditions in a Humic Cambisol soil. The experiment was conducted in a completely randomized design with factorial arrangement (hydrogel and irrigation frequency). The hydrogel factor was composed by the presence or absence of the polymer, and the frequency of irrigation by irrigation only at planting, every 8 days, 4 days and daily. Water stress symptomatology, morphometric aspects, soil water content and gas exchange were evaluated. The hydrogel provided an increase in soil water content and delayed symptoms of water stress, especially when irrigation was less frequent. The addition of the polymer also provided improved seedling quality, more accumulated dry mass. Water stress was a limiting factor for stomatal opening. The lower the water availability, the lower the photosynthesis, stomatal conductance and transpiration values. In general, the results confirm that the addition of hydrogel in the soil optimizes water availability, with the possibility of reducing planting mortality and maximizing plant growth in the initial phase of establishment.

Resumo Objetivo Avaliar a frequência de anomalias congênitas (ACs) estruturais no centro-oeste brasileiro e a associação com fatores de risco maternos. Métodos Estudo prospectivo, observacional, caso-controle, baseado em uma população hospitalar. Foram analisadas gestantes atendidas em um serviço de medicina fetal no Brasil, no período de outubro de 2014 a fevereiro de 2016. Foram analisadas 357 gestantes, dentre as quais 223 tiveram fetos com ACs estruturais (grupo controle) e 134 tiveram fetos estruturalmente normais (grupo controle). A história clínica foi feita antes da consulta de pré-natal, e o diagnóstico da AC estrutural foi realizado por ultrassonografia. Resultados Observou-se uma frequência de 64,27% (n = 223) de gestantes com fetos com ACs estruturais. As ACs estruturais mais frequentes foram as do sistema nervoso central (30,94%), seguidas das anomalias do sistema gênito-urinário (23,80%), e, por fim, das ACs múltiplas (16,60%). Antecedentes de crianças com AC (razão de probabiliade [RP]: 3,85; p = 0,022), antecedentes familiares (RP: 6,03; p = < 0,001), e consanguinidade entre os grupos progenitores (RP: 4,43; p = 0,034) influenciaram a ocorrência de AC estrutural. Conclusão As ACs mais frequentes foram as do sistema nervoso central, as do sistema gênito-urinário, e as ACs múltiplas. Os fatores de risco maternos que podem ter influenciado a ocorrência de AC estrutural foram antecedentes de crianças com AC, história familiar, e a consanguinidade entre os pais.

Abstract Objective To evaluate the frequency of structural congenital anomalies (CAs) in the midwest of Brazil and its association with maternal risk factors. Methods This was a prospective, observational, case-control study based on a hospital population. Pregnant women attended at a fetal medicine service in Brazil were analyzed in the period from October 2014 to February 2016.A total of 357 pregnant women were included, 223 of whom had fetuses with structural anomalies (group case), and 134 of whom had structurally normal fetuses (control group). The clinical history was made previous to prenatal consultation, and the diagnosis of the structural CA was performed through ultrasound. Results A frequency of 64.27% (n = 223) of pregnant women with fetuses with structural anomalies was observed. The most frequent structural CAs were those of the central nervous system (30.94%), followed by anomalies of the genitourinary system (23.80%), and, finally, by multiple CAs (16.60%). The background of previous children with CAs (odds ratio [OR]: 3.85; p = 0.022), family history (OR: 6.03; p = < 0.001), and consanguinity between the progenitors (OR: 4.43; p = 0.034) influenced the occurrence of structural CA. Conclusion The most frequent CAs are those of the central nervous system, followed by those of the genitourinary system, and then multiple anomalies. The maternal risk factors that may have influenced the occurrence of structural CA were previous children with CA, family history, and consanguinity among the parents.

Abstract Introduction: obesity is increasing among women at reproductive age in Brazil. Excess body weight during pregnancy negatively impacts women's health. Objectives: to identify and analyze the publications that showed the effects of pregestational excess weight on pregnancy, delivery, and post-delivery in Brazilian women. Methods: this systematic review was performed including studies that involve Brazilian pregnant women with adverse outcomes caused by pregestational excess weight. Search, selection, and reporting were conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement. The study was conducted by manually searching and screening the databases LILACS, PubMed, Scopus, Cochrane, and Periodicos CAPES. The selected articles were evaluated according to the quality of evidence using the Grading of Recommendations, Assessment, Development and Evaluation (GRADE), and categorized as studies with high (A), moderate (B), low (C), or extremely low (D) evidence quality. Results: a total of 1,582 studies were found, of which 39 were included for final reading and evaluation. Among these, 12.8 %, 69.2 %, and 18.0 % were classified as A, B, and C or D for evidence quality, respectively. Hypertensive disorders of pregnancy, caesarean section, excessive weight gain, and gestational diabetes mellitus were commonly associated with pregestational excess weight in Brazilian women. Conclusions: the negative effects of excess body weight during pregnancy reflect the need for effective public policies that can address the problem, focusing on interventions that promote the health of women at reproductive age.

Resumen Introducción: la obesidad está aumentando entre las mujeres en edad reproductiva en Brasil. El exceso de peso corporal durante el embarazo afecta negativamente a la salud de las mujeres. Objetivos: identificar y analizar publicaciones que mostraran los efectos del exceso de peso pregestacional sobre el embarazo, el parto y el posparto en mujeres brasileñas. Métodos: esta revisión sistemática incluye estudios de mujeres embarazadas brasileñas con resultados adversos causados por el exceso de peso pregestacional. La búsqueda, la selección y la presentación de los resultados se realizaron de acuerdo con el sistema Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). El estudio se realizó mediante la búsqueda manual y el cribado de las bases de datos LILACS, PubMed, Scopus, Cochrane y Periódicos CAPES. Los artículos seleccionados se evaluaron de acuerdo con la calidad de la evidencia utilizando el sistema Grading of Recommendations, Assessment, Development and Evaluation (GRADE), y se clasificaron como estudios de alta (A), moderada (B), baja (C) o extremadamente baja (D) calidad de la evidencia. Resultados: se encontraron un total de 1582 estudios, de los cuales 39 se incluyeron para su lectura final y evaluación. Entre estos, el 12,8 %, 69,2 % y 18,0 % se clasificaron como A, B y C o D en cuanto a calidad de evidencia, respectivamente. Los trastornos hipertensivos del embarazo, la cesárea, el aumento de peso excesivo y la diabetes mellitus gestacional se asociaron comúnmente con el exceso de peso pregestacional en las mujeres brasileñas. Conclusiones: los efectos negativos del exceso de peso corporal durante el embarazo reflejan la necesidad de políticas públicas efectivas que puedan abordar el problema, centrándose en intervenciones que promuevan la salud de las mujeres en edad reproductiva.

ABSTRACT Sequoia sempervirens is different from other species not only for its excellent vigor and longevity, but also for quality wood productivity. The objective of this work was to evaluate the rooting of Sequoia sempervirens mini-cuttings in different cultivation environments. In both experiments, rooting was evaluated in time to determine the root development in each environment. Mini-cuttings from clone A228 from clonal mini-garden were used. In the first experiment the material was placed to take root in three different environments: greenhouse (GH), shadehouse (SH) and mini-tunnel, with evaluation performed every 30 days (from 30 to 150 days). In the second experiment, we compared the development and rooting in mini-tunnel, greenhouse with intermittent mist (GWIM), with weekly evaluations (from 30 to 93 days). Both experiments were installed in a completely randomized design, with subsequent evaluation of the survival percentage, percentage of rooted cuttings and number of roots emitted by rooted cuttings. Humidity and temperature data were also collected in all environments observed. The mini-tunnel was the most efficient place for sequoia mini-cutting, presenting higher survival, rooting and number of roots when compared to the shadehouse and the greenhouse. In addition, it provided rooting similar to the greenhouse with intermittent mist (close to 90%). It is recommended to keep mini-cuttings in a mini-tunnel or greenhouse with intermittent mist for a period of 90 days for proper rooting to occur. Good temperature control was also observed in this structure, as well as excellent humidity control. The mini-tunnel can be used for sequoia propagation by mini-cuttings as it has shown good rooting results (> 80%).

RESUMO Pesquisas com nutrição de plantas são essenciais para a correta fertilização, melhorando produtividade e qualidade do produto, além de poder contribuir para a atualização da recomendação. O objetivo do experimento foi avaliar o crescimento, acúmulo e exportação de nutrientes, soma térmica e produtividade da cultivar Corona F1. O trabalho foi conduzido no período de 2 de agosto a 25 de outubro de 2013, em Santo Antônio de Posse-SP. Ao final do experimento, 84 dias após o transplante (DAT), a altura média observada foi de 95 cm com 36 folhas e produtividade máxima obtida de 45,8 t ha-1. Concluiu-se que o acúmulo de nutrientes, em g planta-1, foi de: N (14,2) > K (9,3) > Ca (8,0) > Mg (2,5) > S (1,2) > P (0,9) e em mg planta-1, de Mn (49,3) > Fe (34,9) > Zn (19,6) > B (16,4) > Cu (2,1) e a exportação, em kg ha-1, foi de N (94,9) > K (44,1) > Ca (43,0) > Mg (12,6) > S (6,3 ) > P (5,3), em g ha-1, Mn (271,0) > Fe (159,2) > Zn (105,6) > B (82,8) > Cu (11,9). A maior demanda por nutrientes ocorreu entre 40 e 50 DAT. A cultivar Corona F1 precisou de 1.538 graus dias para completar seu ciclo.

ABSTRACT Studies on plant nutrition are essential for a correct fertilizer management, improving yield and quality of the product, and it also may contribute for an updating recommendation. The aim of this study was to evaluate growth, nutrient accumulation and export, thermal sum and yield of zucchini cultivar Corona F1. The experiment was carried out from August 8 to October 25, 2013, in Santo Antônio de Posse, São Paulo State. At the end of the research, 84 days after transplanting (DAT), we observed 95 cm average height with 36 leaves and 45.8 t ha-1 maximum yield. The nutrient accumulation, in g plant-1, was: N (14.2) > K (9.3) > Ca (8.0) > Mg (2.5) > S (1.2) > P (0.9) and in mg plant-1, Mn (49.3) > Fe (34.9) > Zn (19.6) > B (16.4) > Cu (2.1) and export, in kg ha-1, was N (94.9) > K (44.1) > Ca (43.0) > Mg (12.6) > S (6.3 ) > P (5.3), in g ha-1, Mn (271.0) > Fe (159.2) > Zn (105.6) > B (82.8) > Cu (11.9). The highest nutrient demand occurred between 40 and 50 DAT. Cultivar Corona F1 cycle was completed within 1.538 degree-days.

Resumo Objetivo Avaliar e comparar a prevalência de anomalias congênitas (ACs) estruturais de acordo com o índice de massa corporal (IMC) materno. Métodos Estudo transversal envolvendo gestantes com fetos diagnosticados com ACs estruturais por ultrassonografia morfológica entre novembro de 2014 e janeiro de 2016. O estado nutricional das gestantes foi classificado de acordo como valor bruto do índice de massa corporal. As gestantes foram categorizadas em quatro grupos: baixo peso, peso adequado, sobrepeso e obesidade. A análise estatística foi realizada no programa Stata/SE versão 12.0 (Stata Corporation, College Station, TX), comvalores de p ≤ 0,05 considerados estatisticamente significantes. Resultados Um total de 223 gestantes tiveram fetos diagnosticados com ACs. A prevalência de AC estrutural emgestantes combaixo peso foi de 20,18%, emgestantes com peso adequado foi de 43,50%, em gestantes com sobrepeso foi de 22,87%, e em gestantes com obesidade foi de 13,45%. A prevalência de anomalias do sistema nervoso central (SNC) e do sistema geniturinário foi alta para os quatro grupos. Observou-se associação positiva entre múltiplas anomalias em gestantes com peso adequado (razão de prevalência [RP] = 1,65; p ≤ 0,004) e entre anomalias do sistema linfático em gestantes obesas (RP = 4,04, p ≤ 0,000). Conclusão A prevalência das anomalias do SNC e do sistema geniturinário foi alta em todas as categorias de IMC. Gestantes obesas foram associadas a anomalias do sistema linfático. Portanto, o rastreamento e a identificação dos fatores de risco para as AC são importantes, independentemente do IMC materno. Nossos achados reforçam a importância de discutir com gestantes sobre a nutrição materna e seu efeito no desenvolvimento fetal e no desfecho neonatal.

Abstract Objective To evaluate and compare the prevalence of structural congenital anomalies (CAs) according to maternal body mass index (BMI). Methods The present cross-sectional study involved pregnant women with fetuses diagnosed with structural CAs through morphological ultrasonography between November 2014 and January 2016. The nutritional status of the pregnant women was classified according to the gross value of the body mass index. The pregnant women were categorized into four groups: low weight, adequate weight, overweight, and obesity. Statistical analysis was performed using Stata/SE version 12.0 (Stata Corporation, College Station, TX), with values of p ≤ 0.05 considered statistically significant. Results A total of 223 pregnant women had fetuses diagnosed with CAs. The prevalence of structural CAs in pregnant women with lowweight was of 20.18%, of 43.50% in pregnant women with adequate weight, of 22.87% in pregnant women with overweight, and of 13.45% in pregnant women with obesity. The prevalence of central nervous system (CNS) anomalies and of genitourinary systemanomalieswas high for the four groups of pregnant women. A positive association was observed between multiple anomalies in pregnant women with adequate weight (prevalence ratio [PR] = 1.65; p ≤ 0.004) and between anomalies of the lymphatic system in obese pregnant women (PR = 4.04, p ≤ 0.000). Conclusion The prevalence of CNS and genitourinary systemanomalies was high in all of the BMI categories. Obese pregnancies were associated with lymphatic system anomalies. Therefore, screening and identification of the risk factors for CAs are important, regardless of the maternal BMI. Our findings reinforce the importance of discussing with pregnant women maternal nutrition and its effect on fetal development and on neonatal outcome.

Resumo: O objetivo deste trabalho foi avaliar o cultivo de melancia (Citrullus lanatus) em áreas de renovação de canavial, em diferentes sistemas de preparo do solo e adubação nitrogenada. Foram realizados dois experimentos nas safras de 2014/2015 e 2015/2016, em áreas de plantio de cana-de-açúcar, em Andradina, no Estado de São Paulo, Brasil. Os cultivos foram feitos em delineamento experimental de blocos ao acaso, com parcelas, subparcelas e quatro repetições. As parcelas representaram os sistemas de preparo do solo (plantio convencional, cultivo mínimo e plantio direto), e as subparcelas, as diferentes doses de N aplicadas em cobertura (0, 100, 200 e 300 kg ha-1). Em 2014/2015, o cultivo mínimo apresentou a maior produtividade comercial de 70,2 Mg ha-1. Em 2015/2016, não houve diferenças quanto à produção entre os tratamentos de preparo de solo; porém, a produção diferiu entre os tratamentos com N. As maiores produtividades comerciais de 64,1 e 31,1 Mg ha-1 foram obtidas com as doses de 253 e 209 kg ha-1 de N em cobertura, respectivamente, em 2014/2015 e 2015/2016. A melancia pode ser cultivada em áreas de renovação de canavial, e a demanda de N pela planta não depende do sistema de preparo de solo.

Abstract: The objective of this work was to evaluate watermelon (Citrullus lanatus) cultivation in regeneration areas of a sugarcane field, under different soil management systems and N fertilization regimes. Two experiments were carried out in the 2014/2015 and 2015/2016 harvest seasons, in areas of sugarcane plantation in Andradina, in the state of São Paulo, Brazil. Cultivations were performed in a randomized complete block design, with plots and subplots, and four replicates. The plots represented the tillage systems (conventional, minimum tillage, and no-tillage), and the subplots, the different N fertilization rates (0, 100, 200, and 300 kg ha-1) applied as topdressing. In 2014/2015, the minimum tillage system resulted in the highest commercial yield of 70.2 Mg ha-1. In 2015/2016, there were no differences for yield among tillage systems; however, yield differed among N treatments. The highest commercial yields of 64.1 and 31.1 Mg ha-1 were achieved with the N doses of 253 and 209 kg ha-1 as topdressing, respectively, in 2014/2015 and 2015/2016. Watermelon can be cultivated in regeneration areas of sugarcane field, and the demand of N by the plant does not depend on the soil tillage system.

RESUMO Novos híbridos de cebola mais produtivos estão sendo cultivados atualmente. Assim, informações sobre o crescimento e absorção de nutrientes devem ser atualizadas para auxiliar no refinamento das recomendações existentes, possibilitando melhor explorar o potencial produtivo desses novos genótipos. Objetivou-se com o trabalho determinar o crescimento, acúmulo e exportação de nutrientes do híbrido de cebola Soberana no sistema de semeadura direta. O delineamento experimental foi o de blocos casualizados, com quatro repetições, onde os tratamentos foram épocas de avaliação. Avaliou-se o número de folhas, massa seca, acúmulo de nutrientes e a taxa máxima de acumulo diário. A sequência de acúmulo de nutrientes foi (g planta-1): K (0,72) > Ca (0,38) > N (0,32) > S (0,14) > P (0,08) > Mg (0,06) e (mg planta-1) Fe (2,26) > Mn (1,43) > Cu (0,93) > Zn (0,91) > B (0,85). A exigência máxima dos macronutrientes ocorreu entre 61 e 148 dias após a semeadura (DAS) e dos micronutrientes entre 70 e 148 DAS.

ABSTRACT New hybrid onions that are more productive are currently being cultivated. Information on growth and nutrient accumulation must thus be updated to assist in the refinement of existing recommendations, enabling the better exploitation of the productive potential of these new genotypes. We determined the growth of plants and the accumulation and export of nutrients of the short-day onion ‘Soberana’ established by direct seeding. The experimental design was a randomized block with four replicates and the treatments were evaluation times. Leaf number, tissue dry weight, nutrient accumulation, and the maximum daily rate of accumulation were evaluated throughout the growing cycle. The order of nutrient accumulation was (g plant-1) K (0.72) > Ca (0.38) > N (0.32) > S (0.14) > P (0.08) > Mg (0.06) and (mg plant-1) Fe (2.26) > Mn (1.43) > Cu (0.93) > Zn (0.91) > B (0.85). Macronutrient demand was highest between 61 and 148 days after sowing (DAS), and micronutrient demand was highest between 70 and 148 DAS.