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ABSTRACT Enteropatogenic Escherichia coli (EPEC) and shigatoxigenic E. coli (STEC), are generally poultry and poultry product isolate and can cause serious human infections. Many strains may become resistant to various antimicrobials, which can hinder the treatment of bacterial diseases. Organic farming seeks to avoid the selection and frequency of antimicrobial-resistant bacteria. This study aims to verify the resistance of EPEC and STEC from organic and conventional (industrial) broiler isolates to antimicrobials. All isolates were submitted to disk diffusion test with tetracycline, gentamicin, enrofloxacin, ceftriaxone and amoxicillin + clavulanate (TET, GEN, ENO, CTX, AMC) and PCR to detect specific virulence genes for EPEC and STEC. A total of 297 E. coli strains were isolated, 213 from conventional. In organic broiler, 84 strains were isolated. The strains from the conventional broiler isolates were resistant to five antimicrobials tested: TET 48.82% (104/213), ENO 28.17% (60/213), CTX 15.49% (33/213), GEN 14.55% (31/213), and AMC 7.04% (15/213), and 9.86% (21/213) were considered multidrug-resistant. Organic chicken strains were resistant to four of the antimicrobials tested: TET 35.7% (30/84), ENO 9.5% (8/84), CTX 2.4% (2/84), GEN 4.8% (4/84). Of the strains from the organic broiler chicken isolates, only 1.2% (1/84) was considered multidrug-resistant. No EPEC and STEC were found in the organic chicken samples. The multidrug resistance was characterized in 9.52% (2/21) of the EPEC and 4.76% (1/21) of the STEC. The study demonstrated the absence of EPEC and STEC strains in organic broilers and carcasses and a lower frequency of multiresistant strains compared to conventional breeding. (EPEC E STEC, , (STEC) infections diseases antimicrobialresistant antimicrobial bacteria industrial (industrial tetracycline gentamicin enrofloxacin TET, (TET 29 isolated 21 8 tested 4882 48 82 48.82 104/213, 104213 104/213 104 (104/213) 2817 28 17 28.17 60/213, 60213 60/213 60 (60/213) 1549 15 49 15.49 33/213, 33213 33/213 33 (33/213) 1455 14 55 14.55 31/213, 31213 31/213 31 (31/213) 704 7 04 7.04 15/213, 15213 15/213 (15/213) 986 9 86 9.86 21/213 21213 (21/213 multidrugresistant. multidrugresistant resistant. multidrug-resistant 357 35 35.7 30/84, 3084 30/84 30 (30/84) 95 5 9.5 8/84, 884 8/84 (8/84) 24 2 4 2.4 2/84, 284 2/84 (2/84) 4.8 4/84. 484 4/84 . (4/84) 12 1 1.2 1/84 184 (1/84 samples 952 52 9.52 2/21 221 (2/21 476 76 4.76 1/21 121 (1/21 breeding (STEC 488 48.8 10421 104/21 10 (104/213 281 28.1 6021 60/21 6 (60/213 154 15.4 3321 33/21 3 (33/213 145 14.5 3121 31/21 (31/213 70 0 7.0 1521 15/21 (15/213 98 9.8 21/21 2121 (21/21 35. 308 30/8 (30/84 9. 88 8/8 (8/84 2. 2/8 (2/84 4. 4/8 (4/84 1. 1/8 18 (1/8 2/2 22 (2/2 47 4.7 1/2 (1/2 48. 1042 104/2 (104/21 28. 602 60/2 (60/21 15. 332 33/2 (33/21 14. 312 31/2 (31/21 7. 152 15/2 (15/21 21/2 212 (21/2 30/ (30/8 8/ (8/8 2/ (2/8 4/ (4/8 1/ (1/ (2/ 104/ (104/2 60/ (60/2 33/ (33/2 31/ (31/2 15/ (15/2 21/ (21/ (30/ (8/ (4/ (1 (2 (104/ (60/ (33/ (31/ (15/ (21 (30 (8 (4 ( (104 (60 (33 (31 (15 (3 (10 (6