RESUMO: A distribuição espacial e a abundância de carrapatos em estágio de vida livre foram examinadas em fragmentos de Mata Atlântica no Nordeste do Brasil. O estudo foi realizado em Unidades de Conservação de Proteção Integral Mata do Tapacurá e Mata do Camucim, localizadas no município de São Lourenço da Mata, Pernambuco. Os carrapatos foram capturados pelo método da bandeira de flanela em seis parcelas de 200m2, sendo três parcelas estabelecidas em uma borda de mata adjacente a uma matriz agropastoril e outras três parcelas estabelecidas na borda de mata adjacente ao lago de barragem. Foram capturados 2652 carrapatos do gênero Amblyomma, sendo duas ninfas identificadas como Amblyomma dissimile com distribuição espacialmente agregada, demonstrados pela relação variância/média e o índice de agregação de Lloyd. Houve variação significativa na abundância de carrapatos entre as parcelas (H=26,79, p<0,005) sendo encontrados mais carrapatos na borda da mata adjacente à matriz agropastoril. Quanto as variáveis ambientais, houve correlação positiva entre a cobertura de dossel e a densidade de carrapatos (rs=0,31, p<0,05). No presente estudo observou-se que em estágios de vida livre, notadamente larvas e ninfas, a distribuição espacial e a abundância de carrapatos são fortemente influenciadas por áreas mais fechadas das matas. A maior presença de carrapatos próximos à matriz agropastoril poderia facilitar a migração de carrapatos entre ambientes silvestres e ambientes antrópicos.

ABSTRACT: The spatial distribution and abundance of free-living ticks were examined in Atlantic Forest fragments in northeastern Brazil. The study was conducted on Integral Protection Conservation Units of Mata do Tapacurá and Mata do Camucim, located in the municipality of São Lourenço da Mata, Pernambuco. Ticks were captured by flagging method in six plots of 200m2, three plots set in a forest edge adjacent to an agropastoral matrix and three plots set in the forest edge adjacent to a dam lake. Were captured 2652 ticks of the genus Amblyomma, two nymphs identified as Amblyomma dissimile with spatially aggregated distribution, shown by the variance/mean ratio and Lloyd aggregate index. There was significant variation in the abundance of ticks between plots (H=26.79, p<0.005) and more ticks was found in the woods adjacent to the edge agropastoral matrix. There was a positive correlation between canopy cover and tick density (rs=0.31, p<0.05). In the present study, it was observed that in free-living stages, especially larvae and nymphs, the spatial distribution and abundance of ticks are strongly influenced by more enclosed areas of forests. Most ticks near agropastoral matrix could facilitate the migration of ticks between wild environments and man-made or modified environments.

ABSTRACT In the semi-arid environment, the reproductive success of anurans depends on adaptations in their life cycle, which synchronizes with ideal environmental conditions to maximize the number of offspring. In this study changes in the histological and morphometric aspects of the testes of Leptodactylus macrosternum Miranda-Ribeiro, 1926 are characterized, to evaluate the influence of rainfall and temperature on them. Specimens were collected at Horto Florestal Olho d’Água da Bica - HFOB (06°49’20”S, 36°15’85”W) area, municipality of Cuité, state of Paraíba, Brazil. Search for specimens was active, happened at night, and amounted to 15 days from January to December 2013. The densities of spermatids (primary and secondary), sperm and area of the seminiferous locules of the testes were registered. The influence of climate variables (rainfall and temperature) on the density of primary and secondary spermatids, sperm and locular area were verified using Simple Linear Regression. Primary spermatids had the lowest density in July (57.90 ± 51.54 mm2), with a peak in November (300.32 ± 117.35 mm2); secondary spermatids had the lowest density in December (287.87 ± 79.05 mm2), with a peak in May (135,727.00 ± 301.13 mm2); sperm was in the lowest density in July (237.37 ± 121.10 mm2), with a peak in June (2,270.45 ± 602.62 mm2) and the locular area had the lowest density in December (40,292.9 ± 8,174.20 µm2) and highest density in June (338,875.01 ± 2,262.10 µm2). A notable decrease in sperm density was evident between June and July. That, associated with the observation of a larger locular area in June, allowed us to identify as June as the peak of spermatogenesis and the following month as the most potentially reproductive. The density of secondary spermatids(r = 0.02), sperm (r = 0.21) and locular area (r = 0.01) showed dependency on rainfall whereas only sperm (r = 0.09) showed dependency on temperature. Therefore, we can state that the reproductive cycle of L. macrosternum is potentially continuous, with a reproductive peak in July.

ABSTRACT The Romualdo Member of the Santana Formation, a lithostatigraphic unit attributed to a marine intrusion, is famous for its preserved fossils in calcareous concretions, which stand out for their diversity and excellent preservation levels. This paper aims to record the first occurrence of the Class Insecta in the Romualdo Member of the Santana Formation of the Araripe Basin, as well as to describe and discuss the paleo-ecological implications of such finding. The first occurrence of the order Orthoptera (family Gryllidae) is presented for this unit. This new species is attributed to the genus Araripegryllus, that lasted throughout the deposition of the Crato Member, which is under the Romualdo Member. In reference to its statigraphic origin, the specimens was named Araripegryllus romualdoi sp. nov.