Abstract Objective: To investigate the clinical implications of Golgi glycoprotein 73 (GP73) and granulocyte colony-stimulating factor (G-CSF) in children with bronchopneumonia (BP). Methods: Seventy-two children with BP (observation group) and 81 healthy children (control group) consecutively brought to the present study's hospital between June 2019 and October 2020 were enrolled. GP73 and G-CSF levels were determined to analyze their diagnostic value for pediatric BP. High-sensitivity C-reactive protein (hs-CRP) was also measured. The clinical implications of GP73 and G-CSF in pediatric BP complicated with respiratory failure and their connections with the inflammatory response were discussed. Results: GP73 and G-CSF levels were remarkably higher in the observation group (p< 0.05). The sensitivity and specificity of combined detection (GP73+G-CSF) in predicting pediatric BP were 72.22% and 86.42%, respectively (p < 0.001 ). GP73 and G-CSF, which are closely related to X-ray classification and complications in the observation group, decreased after treatment and were positively correlated with hs-CRP (p < 0.05), especially in children complicated with respiratory failure. Regression analysis identified the independence of the course of the disease, hs-CRP, X-ray classification, GP73, and G-CSF as influencing factors of respiratory failure in children with BP (p < 0.05). Conclusion: GP73 and G-CSF, with elevated levels in children with BP, are strongly linked to disease progression and are independent influencing factors of respiratory failure, which may be the key to diagnosing and treating pediatric BP in the future.

ABSTRACT Introduction: Early special training has been widely used to improve athletic performance in young Chinese boxing athletes. For athletes to make better use of training time and accumulate maximum special competitive skills, intensive training is required from their youth. Objective: Study the competitive ability of Chinese boxing athletes under strengthening through early physical training. Methods: Through literature method, experimental methods, and mathematical statistical analysis, the athletes’ body composition and their performance in specific and functional physical activities were analyzed. results: Young athletes significantly differed in BMI, height, and limb length (P<0.05). There was no significant difference in height, the distance between fingers, Achilles tendon length (P>0.05). No significant difference was found in flexibility and sensitivity among volunteers (P>0.05). Conclusion: The athletes participating in early training are uniformly matched, the level of tactical ability of the athletes under training has a differential that can directly impact future sports performance, and this practice is recommended to improve the athletes’ sports performance. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMEN Introducción: El entrenamiento especial temprano se ha utilizado ampliamente con el objetivo de mejorar el rendimiento deportivo en los jóvenes atletas de boxeo chinos. Para que los deportistas aprovechen mejor su tiempo de entrenamiento y acumulen el máximo de habilidades competitivas especiales, es necesario un entrenamiento intensivo desde su juventud. Objetivo: Estudiar la capacidad competitiva de los atletas chinos de boxeo bajo fortalecimiento a través del entrenamiento físico temprano. Métodos: Mediante el método bibliográfico, los métodos experimentales y el análisis estadístico matemático, se analizó la composición corporal de los atletas y su rendimiento en actividades físicas específicas y funcionales. Resultados: Hubo diferencias significativas en el IMC, la altura y la longitud de las extremidades entre los jóvenes atletas (P<0,05). No hubo diferencias significativas en la altura, la distancia entre los dedos y la longitud del tendón de Aquiles (P>0,05). No se encontraron diferencias significativas en la flexibilidad y la sensibilidad entre los voluntarios (P>0,05). Conclusión: Los atletas que participan en el entrenamiento temprano están uniformemente emparejados, el nivel de habilidad táctica de los atletas en entrenamiento tiene un diferencial que puede impactar directamente en el futuro rendimiento deportivo, siendo recomendada esta práctica para mejorar el rendimiento deportivo de los atletas. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

RESUMO Introdução: O treinamento especial precoce tem sido amplamente utilizado visando melhorar o desempenho atlético em jovens atletas de boxe chinês. No intuito dos atletas aproveitarem melhor o tempo de treino e acumular o máximo de habilidades competitivas especiais, é requerido um treinamento intensivo desde sua juventude. Objetivo: Estudar a capacidade competitiva dos atletas de boxe chinês sob o fortalecimento através do treinamento físico precoce. Métodos: Através do método de literatura, métodos experimentais e análise estatística matemática, analisou-se a composição corporal dos atletas, seu desempenho em atividades físicas específicas e funcionais. Resultados: Houve diferenças significativas no IMC, altura e comprimento de membros entre os jovens atletas (P<0,05). Não houve diferença significativa na altura, distância entre os dedos, comprimento do tendão de Aquiles (P>0,05). Não foi encontrada diferença significativa na flexibilidade e na sensibilidade entre os voluntários (P>0,05). Conclusão: Os atletas participantes do treinamento precoce são uniformemente equiparados, o nível de habilidade tática dos atletas sob capacitação nesse treinamento tem um diferencial que pode impactar diretamente sobre o desempenho esportivo futuro, sendo recomendada essa prática para melhorar o desempenho esportivo dos atletas. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

SUMMARY OBJECTIVE: This study aimed to explore the correlation between different body components and bone mineral density in healthy adults. METHODS: A total of 306 non-manual subjects, 161 males and 145 females, were selected from the physical examination center of our hospital from June to September 2019. They were divided into control group, overweight group, and obese group according to body mass index. The muscle mass and fat mass, body fat content, trunk fat mass, upper limb and thigh fat mass, bone density of femoral neck and lumbar vertebra, and bone mineral salt content of the whole body were measured by dual-energy X-ray absorptiometry. RESULTS: Body mass index, systolic blood pressure, diastolic blood pressure, femoral neck bone mineral density, bone mineral salt content, fat mass, muscle mass, upper limb fat mass, thigh fat mass, and trunk fat mass in the overweight group and obese group were all higher than those in the control group (P<0.05). The fat mass, muscle mass, upper limb fat mass, and trunk fat mass were positively correlated with the femoral neck bone mineral density, total lumbar vertebra bone mineral density, and bone mineral salt content (P<0.05). In addition, thigh fat mass was positively correlated with femoral neck bone mineral density and total lumbar spine bone mineral density, whereas body fat content was negatively correlated with bone mineral salt content. CONCLUSION: Body composition was related to bone mineral density and bone mineral salt content, and the correlation between different body composition indexes, and bone mineral density, and bone mineral salt content was different.

ABSTRACT Soil moisture (SM) plays an important role in regulating the global water cycle, especially in arid areas, and is one of the main indicators of ecological environmental health. Although traditional methods can accurately measure SM at a single sample site, they are limited in large-scale and dynamic SM monitoring. Therefore, we used the Landsat images as the data source and the soil adjusted vegetation index (SAVI) to build the adjusted SAVI (aSAVI) index by modifying the soil adjustment parameter L and introducing the short-wave infrared band. According to the theory of temperature vegetation dryness index (TVDI) and feature space, we introduced a model, combined the measured SM data (Minqin Basin, China) through a comparative analysis of four vegetation indices (NDVI, SAVI, MSAVI, aSAVI) determine the optimal model. Taking the Minqin Basin as the study area, the spatiotemporal variation characteristics of SM in three sub-regions (the entire study area, irrigated region, and periphery of the irrigated regions) were quantitatively analyzed and compared in four different periods: pre-Comprehensive Treatment Program of the Shiyang River Basin (pre-CTSRB) (2000–2005), CTSRB I (2006–2010), CTSRB II (2011–2016), and CTSRB-end (2017–2021) to evaluate the ecological restoration effects of treatment programs from the SM perspective. The results showed that: 1) SM values derived from TVDI inversion and the aSAVI were more accurate, and the model sensitivity decreased with soil depth; 2) the mean value of SM fluctuated across the four periods but decreased slightly over the entire time series. The spatial variations of the SM were characterized by a “descending then ascending” trend. Soil moisture increased in 21.35 % of areas at 0.00-0.10 m in the past 22 years, and 59.66 % at 0.10-0.20 m. There was a negative correlation between the mean variation trend of SM and the percentage of area where SM fell in different periods; 3) the treatment program positively affected the ecological restoration of the Minqin Basin from the SM perspective. The area where SM increased was larger than that of decreasing SM, especially in 0.10-0.20 m soil layer. The increase can promote growth and confer resistance to desertification.

OBJECTIVES: To investigate the role of miR-139-5p and the TLR4/MyD88/NF-κB signaling pathway in acute lung injury in septic mice. METHOD: A total of 140 healthy male SPF C57BL/6 mice were divided into seven groups, i.e., Normal, Control, NC, miR-139-5p mimic, miR-139-5p inhibitor, TAK-242, and miR-139-5p inhibitor+TAK-242 groups. The levels of miR-139-5p, proteins related to the TLR4/MyD88/NF-κB signaling pathway (TLR4, MyD88, and p-NF-κB p50), and MPO, SOD, GSH, and MDA in lung tissue were measured. The lung tissue wet-to-dry mass ratio (W/D), arterial oxygen partial pressure (PaO2), and carbon dioxide partial pressure (PaCO2) were measured. RESULTS: A web-based bioinformatic tool predicted that MyD88 was a target of miR-139-5p, which was verified by a dual luciferase reporter assay. Compared with those in the Normal group, the levels of miR-139-5p, PaO2, SOD, and GSH were significantly lower, while those of TLR4, MyD88, p-NF-κB p50, W/D, PaCO2, IL-1β, TNF-α, IL-6, MPO, and MDA were higher in all other groups. Moreover, compared with their levels in the Control group, these indicators exhibited contrasting results in the miR-139-5p mimic and TAK-242 groups, but were similar in the miR-139-5p inhibitor group. In the miR-139-5p inhibitor+TAK-242 group, acute lung injury, aggravated by miR-139-5p inhibitor, was partially rescued by TAK-242. CONCLUSION: miR-139-5p inhibits the TLR4/MyD88/NF-κB signaling pathway to alleviate acute lung injury in septic mice.

Abstract This study aimed to elucidate the anti-inflammatory and antioxidant properties of resveratrol (RSV) in human gingival fibroblasts (HGFs) following stimulation by P. gingivalis lipopolysaccharide (LPS). The levels of the inflammatory cytokines IL-1β, IL-6, IL-8 and TNFα, the activity of the antioxidant enzymes SOD and GSH-Px, and the levels of MDA, were evaluated by ELISA. It was observed that the expression of IL-1β, IL-6, IL-8 and TNFα in LPS-induced HGFs was significantly downregulated by RSV in a dose-dependent manner. RSV also partly increased oxidative stress (OS)-related factors, including SOD and GSH-Px, which was accompanied by a decrease in MDA production, although the results were not statistically significant. Additionally, RSV-induced deactivation of the PI3K/AKT and Wnt/β-catenin pathways in LPS-induced HGFs was observed by western blot analysis. Subsequently, it was demonstrated treatment with PI3K/AKT pathway inhibitor (LY294002) or Wnt/β-catenin pathway inhibitor (Dickkopf-1, DKK-1) could further enhance the anti-inflammatory and antioxidant effects of RSV by downregulating the expression of IL-1β, IL-6, IL-8 and TNFα, and the production of MDA, and increasing the activity of SOD and GSH-Px in LPS-induced HGFs. These results suggested RSV attenuated the inflammation and OS injury of P. gingivalis LPS-treated HGFs by deactivating the PI3K/AKT and Wnt/β-catenin signaling pathways.

ABSTRACT Objective: The clinical significance of coexistence of HBsAg/anti-HBs in chronic hepatitis B (CHB) patients remains controversial. This study was aimed to assess the association of this serological pattern with hepatocellular carcinoma (HCC) in patients with CHB. Methods: In this cross-section study, 206 CHB patients with coexistence of HBsAg/anti-HBs and 206 CHB patients with HBsAg alone were included to evaluate the risk of HCC development by logistic regression analysis. In addition, a retrospective cohort of 260 patients with CHB was recruited to estimate the cumulative incidence of HCC by Kaplan-Meier analysis. Results: The serological pattern of coexistence of HBsAg/anti-HBs, with high levels of ("High") HBsAg/low levels of ("Low") anti-HBs, were considered as independent risk factors for HCC. In particular, patients with "High" HBsAg/"High" anti-HBs [odds ratio (OR), 4.295; 95% confidence interval (CI), 1.104-16.699; p = 0.035] and "Low" HBsAg/ "High" anti-HBs (OR, 3.207; 95%CI, 1.299-7.919; p = 0.012) exhibited significantly higher risk for HCC development. However, only "Low" HBsAg /"High" anti-HBs might increase risk of HCC in CHB patients with high HBV load (logrank p < 0.001) in our cohort study. Conclusion: The coexistence of "Low" HBsAg /"High" anti-HBs might increase the risk of HCC development in CHB patients with high HBV load, which reflected that the long-term interaction between immune response and virus might lead to the development of HCC. The identification of the patients with poor prognosis will help clinicians to refine the therapeutic decisions and individualize follow-up strategies.

'Stammering tongue' is the governing metaphor we offer in our reading of the border. The border, we read as a central technique of both the modern state and the violence that produces it. Our project is a diffractive encounter with the modality of implicating and complicating reading and writing. The paper offers a reading of two recent texts, Christina Sharpe's In the Wake: On Blackness and Being that draws from the metaphor/practice of the Middle Passage to offer "The Wake," "The Ship," "The Hold," and "The Weather," to theorize black violability, black death, and black living. We read Sharpe beside Jasbir K. Puar's The Right to Maim: Debility, Capacity, Disability where she uses the notion of debility to stress the relations between harm, gender, race, war, and labor. We offer the 'stammering tongue,' in pursuit of a conversation between ourselves, Sharpe, and Puar. The stammering tongue is a racialized, sexualized border that produces (im)possible readings and utterances. We frame the stammering tongue as one that turns to negativity and reclaims lack to generate potentiality from that lack.

The heterogeneity of berry heterogeneity is a commonly occurring phenomenon that has a big influence on fruit composition and wine quality. To clarify this relationship, 'Cabernet Sauvignon' grapes were collected at harvest from a single vineyard and divided into three categories in two consecutive years: small (≤ 0.75 g), medium (0.76-1.25 g), and large (> 1.25 g). The medium berries were present in the highest frequency, accounting for more than 50% of the berry populations. The standard physicochemical parameters of the fruit were significantly affected by berry size. The relative skin mass and soluble solids contents, as well as total phenolic and anthocyanin concentrations, decreased with the berry size, while the relative seed mass, pH and malic acid content were positively correlated with berry weight. Accordingly, the wine composition also varied with berry size, as the wines made from small berries showed the highest alcohol and residual sugar content. CIELab parameters of the resulting wines showed the small berries were more desirable for making wine with a deeper and more saturated colour. With regard to volatile compounds, berry size showed a limited effect. Only 1-hexanol and /aevo-2,3-butanediol showed consistent and significant trends across vintages for wine volatiles, which showed the highest levels in wines made from the small category of berries.

Abstract Engyodontium album is a widespread pathogen that causes different kinds of dermatoses and respiratory tract diseases in humans and animals. In spite of its perniciousness, the basic genetic and molecular background of this species remains poorly understood. In this study, the mitochondrial genome sequence of E. album was determined using a high-throughput sequencing platform. The circular mitogenome was found to be 28,081 nucleotides in length and comprised of 17 protein-coding genes, 24 tRNA genes, and 2 rRNA genes. The nucleotide composition of the genome was A+T-biased (74.13%). Group-II introns were found in the nad1, nad5, and cob genes. The most frequently used codon of protein-coding genes was UAU. Isoleucine was identified as the most common amino acid, while proline was the least common amino acid in protein-coding genes. The gene-arrangement order is nearly the same when compared with other Ascomycota mitogenomes. Phylogenetic relationships based on the shared protein-coding genes revealed that E. album is closely related to the Cordycipitaceae family, with a high-confidence support value (100%). The availability of the mitogenome of E. album will shed light on the molecular systematic and genetic differentiation of this species.

Las Desviaciones de la Vertical (DOV, del inglés Deflections of the Vertical) son información esencial en la simplificación de la observación geodésica, el campo gravitatorio terrestre y el perfeccionamiento del modelo geoide, y el cambio de la masa terrestre. Se estimaron los componentes meridional y principal vertical de las desviaciones de la vertical oceánica global con la información altimétrica de TOPEX/Poseidón, Jason 1 y Jason 2, de 1992 a 2013, con un método de cruce para analizar la distribución espacio-temporal de las desviaciones de la vertical marinas. Comparadas con las desviaciones de la vertical obtenidas con el modelo EGM2008 (Modelo de Gravitación de la Tierra), la precisión de los componentes meridional y principal vertical puede aumentarse 0.98" y 1.02", respectivamente. Se estudiaron las series temporales de la media anual de las desviaciones de la vertical desde 1992 a 2013 para obtener la ley anual de variación. Los cambios anuales de los componentes meridional y principal vertical son muy pequeñas en la mayoría de los océanos. Sin embargo, los cambios anuales son mayores en aquellos océanos con una topografía submarina de grandes ondulaciones. También se analizó la distribución espacial de las desviaciones de la vertical oceánicas. Las desviaciones de la vertical absolutas alrededor de los continentes, islas y fosas oceánicas mayores son superiores que aquellas sobre los otros océanos. Los componentes meridional y vertical principal son consistentes con las direcciones de la longitud y la latitud, respectivamente.

Deflections of the Vertical(DOV) are essential data in the geodetic observation reduce, the earth gravity field and geoid model refinement, and the mass earth change. The meridional and prime vertical components of global oceanic DOVs are estimated from altimetry data of TOPEX/Poseidon, Jason-1, and Jason-2, through 1992 to 2013 with the crossover method, to analyze the temporal-spatial distribution of marine DOVs. Comparing with the EGM2008-modelled DOVs, precisions of meridional and prime vertical components can be up to 0.98” and 1.02”, respectively. The time series of annual mean DOVs from 1992 to 2013 are studied to get the annual variation law. Annual changes of meridional and prime vertical components are small over most oceans. But the annual changes are significantover oceans with large submarine topography undulations.The spatial distribution of oceanic DOVs is also analyzed. The absolute DOVs over oceans around lands, islands, and large oceanic trenches are greater than those over the other oceans. The meridional and prime vertical components are consistent withthe longitude and latitude directions, respectively.

Background The fermentation conditions of recombinant maltose-binding protein fused to neutrophil-activating protein (rMBP-NAP) of Helicobacter pylori were optimized from Escherichia coli TB1 with varying medium, inoculum age and size, time, inducer, pH and temperature in batch fermentation. Results It was revealed that the optimal conditions for the production of rMBP-NAP in shake flask were as follows: M9 medium (with 3% yeast extract powder added), inoculum age of 19 h, inoculum size of 6%, initial pH of 6.6, temperature of 37°C, and 0.7 mmoL/L IPTG inducted 21 h in a 50 mL/250 mL shake flask. The recombinant protein yield was increased from 59 to 592 mg/L after optimization. Fermentation process conducted in a 10 L fermenter with similar conditions could get 30 g/L wet cell and 1.738 g/L soluble protein with the rMBP-NAP expression level of 11.9%. Conclusion The results improve the expression level of rMBP-NAP, and it is expected that these optimized conditions can be well applied for large scale production of rMBP-NAP.

This study aimed to construct a bicistronic DNA vaccine expressing fusion antigen Hsp65-Esat-6 of Mycobacterium tuberculosis with cytokine GM-CSF as a molecular adjuvant (pIRES-Hsp65-ESAT-6-GM-CSF, pIRHEG), and the immune response in mice. C57BL/6 mice were immunized with the recombinant plasmid to detect the titer of antibodies, lymphocyte proliferation, the ratio of CD4+, CD8+T cell and IFN ~ γ，IL-2 secretion. The titer of antibody, lymphocyte proliferation, the ratio of CD4+T and CD8+T cells and IFN ~ γ, IL-2 secretion of pIRHEG group was significant higher than other recombinant plasmid groups, which significant differed by statistical mean. The bicistronic DNA vaccine could induce an effective immune response in mice and could be used as vital ingredient of a new tuberculosis vaccine candidate.