ABSTRACT This study aimed to develop a method to treat and reuse non-biodegradable organophosphorus industrial wastewater using the micro-channel reactor. As an important pharmaceutical and fine chemical intermediate, diethyl hydroxymethylphosphonate (DHP) produces large amounts of industrial wastewater, which poses a serious threat to natural environment and human health. It is of great value to find a treatment method of the DHP industrial wastewater with simple operation, good safety, low cost, and environment friendly. In this study, hydrogen peroxide (H2O2) and DHP industrial wastewater were reacted in the micro-channel reactor. Reaction conditions were optimized by adjusting reaction temperature, reaction time, reaction pressure, and sampling flow rate. The treated wastewater was further neutralized by sodium hydroxide (NaOH). After the oxidation and neutralization reaction of the DHP industrial wastewater, the removal rate of chemical oxygen demand (COD) in treatment wastewater can reach 97.3%. Moreover, after the treated solution being dried, the inorganic phosphate solid was obtained with the phosphorus content as high as 92.1%. It can realize the reuse of phosphorus resources for the DHP industrial wastewater.

RESUMO Este trabalho teve como objetivo desenvolver um método para tratar e reutilizar efluentes industriais organofosforados não biodegradáveis usando o reator de microcanal. Como um importante intermediário farmacêutico e químico fino, o dietil hidroximetilfosfonato (DHP) produz grandes quantidades de efluentes industriais, o que representa uma séria ameaça ao ambiente natural e à saúde humana. É de grande valia encontrar um método de tratamento de efluentes industriais DHP com operação simples, boa segurança, baixo custo e ecologicamente correto. Neste estudo, o peróxido de hidrogênio e o efluente industrial DHP foram reagidos no reator de microcanal. As condições de reação foram otimizadas ajustando-se a temperatura de reação, o tempo de reação, a pressão de reação e a taxa de fluxo de amostragem. O efluente tratado foi posteriormente neutralizado por hidróxido de sódio. Após a reação de oxidação e neutralização das águas residuais industriais DHP, a taxa de remoção da demanda química de oxigênio nas águas residuais de tratamento pode chegar a 97,3%. Além disso, depois da secagem da solução tratada, o fosfato inorgânico sólido foi obtido com teor de fósforo tão alto quanto 92,1%. Pode-se reutilizar recursos de fósforo para as águas residuais industriais DHP.

The aim of this study was to enhance the production of vitamin K2 by using N-methyl-N-nitro-N-nitroso-guanidine (NTG) and low energy ion beam implantation and optimizing the fermentation medium. Mutation resulted in 1.66-fold higher production of vitamin K2 than that of the parentl strain. The production by the mutant BN-P15-11-1was increased 55% and reached 3.593±0.107 mg/L by using the Plackett-Burman and Box-Behnken designs to optimize the fermentation medium. The optimal fermentation culture medium was composed of (g/L) glycerol 69.6, sucrose 34.5, K2HPO4 4.0, peptone 20, yeast extract 25 and fermented at 37 °C and 150 rpm for 72 h. The results showed that the NTG and low energy ion beam implantation mutations and optimizing fermentation medium were effective methods to enhance vitamin K2production.