The essential oil extracted from Anemia tomentosa (EOAT) has shown larvicidal potential against Aedes aegypti, based on methods in vitro and in silico. Chromatographic and spectrometric techniques (gas chromatography-mass spectrometry (CG-MS), gas chromatography-flame ionization detector (GC-FID)), as well as mono and bidimensional nuclear magnetic resonance spectroscopy (NMR) were used to assess 10 essential oil components. Chemical composition of essential oil comprised 87.51% sesquiterpenes, with emphasis on presilphiperfolan-1-ol (42.13%) and silphiperfol-5-ene (19.47%). Larvicidal assay conducted in vitro with EOAT has evidenced potential cytotoxic activity up to 48 h exposure to it; mortality rate observed for A. aegypti larvae exposed to essential oil reached 100%. Study conducted in silico with chemical compounds deriving from the herein investigated plant species has evidenced its potential to inhibit acetylcholinesterase in A. aegypti. Activity of triquinane sesquiterpenes ranging from -6.8 to -8.2 kcal mol-1 stood out in comparison to that of temephos (-7.5 kcal mol-1). Chemical compounds identified in the investigated essential oil presented low human and environmental toxicity, as observed in absorption, distribution, metabolism and excretion, and toxicity (ADMETox) predictions. (EOAT chromatographymass chromatography mass CGMS, CGMS CG MS , (CG-MS) chromatographyflame flame GCFID, GCFID GC FID (GC-FID)) NMR (NMR 1 components 8751 87 51 87.51 presilphiperfolan1ol presilphiperfolanol presilphiperfolan ol 42.13% 4213 42 13 (42.13% silphiperfol5ene silphiperfolene silphiperfol 5 ene 19.47%. 1947 19.47% . 19 47 (19.47%) 4 it A 100 100% 6.8 68 6 8 -6. 8.2 82 2 -8. mol1 mol mol- 7.5 75 7 (-7. mol1. mol-1) absorption distribution excretion ADMETox (ADMETox predictions (CG-MS (GC-FID) 875 87.5 42.13 421 (42.13 194 19.47 (19.47% 6. -6 8. -8 7. (-7 (GC-FID 87. 42.1 (42.1 19.4 (19.47 - (- 42. (42. 19. (19.4 ( (42 (19. (4 (19 (1

ABSTRAT: Feline injection-site sarcomas in felines account for more than 40% of cutaneous and subcutaneous neoplasms in felines. The present study aimed to describe the macroscopic and histomorphological findings of feline application/injection sarcomas. Samples from 31 feline tumors with a history of feline application/inflammation sarcoma were re-evaluated regarding histological subtype, mitotic index and score, depth of tissue invasion, and presence of inflammation considering the location, intensity and predominant cell types. Of the 31 samples from felines diagnosed with sarcoma at the application/inflammation site, 87.15% were cats with no defined breed (NDB), with a mean age of 8.5 years. The predominant anatomical sites were the back and flank/abdomen, both with 29% (9/31), and the prevalent histological subtype was fibrosarcoma at 77.4% (24/31), followed by anaplastic giant cell sarcoma at 12.9% (4/31) and myxosarcoma 9.6% (3/31). The histological grade with the highest number of cases was III (51.6%), followed by Grade II (35.4%) and I (12.9%). The mean of the longest axis measurements varied between the different tumor grades without being significant, with the average being 2.5±2.79cm in Grade I tumors and 3.2±2.28cm in Grade II tumors. and 4.68±2.07cm in Grade III tumors. Necrosis was observed in 74.2% of tumors. The tissue inflammation score was mild to moderate in 58% of cases and severe in 32.2%, with lymphocytic and lymphoplasmacytic infiltrates being prevalent, with 25.8% each, followed by lymphoplasmohistiocytic with 22.6%. The infiltration depth was 38.7% in muscle tissue, followed by 32.2% in the subcutaneous tissue. Pleomorphism was accentuated in 51.6%. Desmoplasia was moderate in 45.1%. Satellite nodules were present in 29% of cases, and 19.4% had macrophages with intracytoplasmic content suggestive of adjuvants. Surgical margins were infiltrated (M1) in 48.4% and narrowed in 25.8% (M2). The anatomical locations observed were different from those recommended by the Vaccine-Associated Feline Sarcoma Task Force (VAFST); in most cases, the adjuvanted macrophage was not present. From this data, we can suggest that sarcomas in felines are not only correlated to the vaccine application, corroborating the hypothesis that any material, whether liquid or solid, and any chronic inflammatory process in the subcutaneous tissue of cats can induce the entity if they are predisposed to do so. The detailed histomorphological data evaluated in this study were key points and provided important information about tumor behavior, being a tool for clinical-oncological decision-making. ABSTRAT injectionsite injection site 40 applicationinjection application 3 applicationinflammation reevaluated re invasion location types 8715 87 15 87.15 NDB, NDB , (NDB) 85 8 5 8. years flankabdomen flank abdomen flank/abdomen 29 9/31, 931 9/31 9 (9/31) 774 77 4 77.4 24/31, 2431 24/31 24 (24/31) 129 12 12.9 4/31 431 (4/31 96 6 9.6 3/31. 331 3/31 . (3/31) 51.6%, 516 51.6% 51 (51.6%) 35.4% 354 35 (35.4% 12.9%. (12.9%) significant 25279cm cm 2 79cm 32228cm 28cm 468207cm 68 07cm 742 74 74.2 58 322 32 258 25 25.8 each 226 22 22.6% 387 38 7 38.7 32.2 451 45 1 45.1% 194 19 19.4 adjuvants M1 M (M1 484 48 48.4 M2. M2 (M2) VaccineAssociated Vaccine Associated VAFST (VAFST) material solid so behavior clinicaloncological clinical oncological decisionmaking. decisionmaking decision making. making decision-making 871 87.1 (NDB 93 9/3 (9/31 77. 243 24/3 (24/31 12. 4/3 43 (4/3 9. 33 3/3 (3/31 51.6 (51.6% 35.4 (35.4 (12.9% 74. 25. 22.6 38. 32. 45.1 19. (M 48. (M2 (VAFST 87. 9/ (9/3 24/ (24/3 4/ (4/ 3/ (3/3 51. (51.6 35. (35. (12.9 22. 45. (9/ (24/ (4 (3/ (51. (35 (12. (9 (24 ( (3 (51 (12 (2 (5 (1

RESUMEN El surgimiento de la denominada curricularización de la extensión universitaria, expresada en un marco legal reciente, al tiempo que refuerza el carácter inductor de la extensión a través de cambios concretos en la investigación y la docencia tradicionales, plantea una serie de desafíos a las instituciones de educación superior para adaptarse a esta nueva realidad. Este artículo se propone promover la reflexión crítica sobre iniciativas de integración entre prácticas pedagógicas y extensionistas en el campo de las Ciencias Públicas. Nuestro objetivo es problematizar y reflexionar sobre los desafíos de la curricularización de la extensión en este campo a la luz de la experiencia de la carrera de Administración Pública en la Facultad de Ciencias Aplicadas de la Universidade Estadual de Campinas (Unicamp), a través del método de observación participante. La carrera ha ido mejorando sus prácticas pedagógicas desde las nuevas Directrices Curriculares Nacionales de 2014, y este movimiento cobra impulso a partir de la resolución de 2018 (CNE/CES nº 7), con la implementación de iniciativas que integran las disciplinas en proyectos de impacto social. universitaria reciente tradicionales realidad Públicas Unicamp, Unicamp , (Unicamp) participante 2014 201 CNE/CES CNECES CNE CES 7, 7 7) social (Unicamp 20 2

ABSTRACT The so-called curricularization of university extension was expressed in a recent legal framework passed in Brazil. It entails concrete changes in traditional research and teaching, which reinforces the inducing characteristic of university extension. However, the curricularization of university extension poses a series of challenges for higher education institutions to adapt to this new reality. This article critically reflects on initiatives integrating pedagogical and extension practices in the Public Field. The objectives are to problematize and reflect on the challenges of curricularization of university extension in this field in light of the experience of the public administration program of the School of Applied Sciences at the University of Campinas (Unicamp), using the participant observation method. The program has been improving its pedagogical practices since the new National Curriculum Guidelines in 2014, and this movement has been strengthened after the Resolution CNE/CES 7/2018, with the implementation of initiatives that connect university courses to social impact projects. socalled so called Brazil teaching However reality Field Unicamp, Unicamp , (Unicamp) method 2014 CNECES CNE CES 72018 7 2018 7/2018 projects (Unicamp 201 7201 7/201 20 720 7/20 2 72 7/2 7/

RESUMO A emergência da chamada curricularização da extensão universitária, expressa em um marco legal recente, ao passo que vem reforçando o caráter indutor da extensão por mudanças concretas na pesquisa e no ensino tradicionais, coloca uma série de desafios para que as instituições de ensino superior (IES) se adaptem a essa nova realidade. É no esforço de promover a reflexão crítica sobre iniciativas de integração entre práticas pedagógicas e extensionistas no Campo de Públicas que este artigo se insere. Nossos objetivos são problematizar e refletir a respeito dos desafios da curricularização da extensão nesse campo à luz da experiência do curso de Administração Pública da Faculdade de Ciências Aplicadas da Universidade Estadual de Campinas (Unicamp) mediante metodologia de observação participante. O curso vem aprimorando suas práticas pedagógicas desde as novas Diretrizes Curriculares Nacionais, de 2014, e esse movimento ganhou impulso após a Resolução CNE/CES nº 7, de 2018, com a implementação de iniciativas que integram disciplinas em projetos de impacto social. universitária recente tradicionais IES (IES realidade insere Unicamp (Unicamp participante Nacionais 2014 CNECES CNE CES 7 2018 social 201 20 2

ABSTRACT The obstacles in Phakopsora pachyrhizi management result especially from susceptible soybean genotypes and resistant fungal strains. The objective of the current study was to evaluate the applicability of the emission of extremely low and specific frequencies by Effatha technology in the soybean Asian rust control, nutrition, and its impact on yield. The in-vivo test followed the detached leaves method, with six treatments: frequencies 1 and 2 individually and in association; the conventional chemical treatment (fungicide azoxystrobin + benzovindiflupyr); and witnesses in presence and absence of the fungus. Frequency 1 relates to inhibition of the enzyme succinate dehydrogenase and 2 to ubiquinone oxidase. In the field, frequencies 1 and 2 associated (with the same fungicidal action of the in-vivo study); nutritional frequency; application of azoxystrobin + benzovindiflupyr + mancozeb, and control without application were evaluated. In vivo, the fungicide provided 85% control of the disease symptoms, against 65% of frequencies 1 and 2 in association, which showed a higher efficiency compared to the isolated frequencies. In the field, the rate of increase of symptoms were reduced by all treatments compared to the control. At the end of the soybean cycle, the conventional fungicide resulted in 33% severity against 56% of frequencies 1 and 2 associated, and 69.2% of the control. The emission of the frequency for increased nutrient efficiency stood out positively on yield in relation to all the other ones. The conventional application provided the highest weight of 1,000 grains, possibly a direct reflection of the better control of the disease. strains nutrition invivo vivo method association benzovindiflupyr) fungus oxidase field study) mancozeb evaluated 85 65 cycle 33 56 692 69 69.2 ones 1000 000 1,00 grains 8 6 3 5 69. 100 00 1,0 10 0 1,

RESUMO: A esporotricose é uma infecção fúngica crônica causada por espécies do gênero Sporothrix. A ocorrência de casos refratários ao tratamento de longo prazo, especialmente na região nasal de gatos, alerta para a importância do estudo de sua patogênese. Este estudo teve como objetivo analisar o processo inflamatório das lesões cutâneas de esporotricose felina refratária ao tratamento, por meio de exames citopatológico e histopalógico, e comparar com aspectos clínicos. Treze gatos com lesões cutâneas refratárias ao tratamento com Itraconazol por mais de um ano foram incluídos no estudo. Amostras de lesões cutâneas foram coletadas para análises citopatológicas, histopatológicas e cultura fúngica. Fragmentos de tecidos foram processados e corados pela hematoxilina-eosina (HE) e Prata Metenamina de Grocott (GMS). Duas apresentações clínicas tiveram maior ocorrência: a forma localizada cutânea em animais com bom estado geral e doença estável (n=9, 69,2%); e a forma disseminada cutânea em gatos com estado geral ruim (n=4, 30,8%). A região nasal (84,6%) foi a localização mais frequente das lesões nos gatos com esporotricose refratária ao tratamento. No estudo citopatológico, felinos com menos de duas lesões, e em bom estado geral, (n=9, 69,2%) revelaram ausência ou leve intensidade de leveduras (até 5 leveduras por campo), menor intensidade de macrófagos e neutrófilos, e maior intensidade de células epitelióides, linfócitos, plasmócitos e eosinófilos. Enquanto, (n=4, 30,8%) dos felinos que apresentavam a forma disseminada da esporotricose associada a um estado geral ruim, revelaram acentuada intensidade de leveduras em sua maioria fagocitadas por acentuado número de macrófagos e neutrófilos. Dos animais com bom estado geral, a maioria (n=6, 66,7%) apresentava maior intensidade de eosinófilos. Na histopatologia, o granuloma supurativo mal formado foi o tipo predominante (n=9, 69,2%) nas lesões de esporotricose felina, e (n=4, 30,8%) foram de granulomas bem formados. Granulomas mal formados apresentaram leve a moderada intensidade fúngica (55,6%) nos animais com bom estado geral e lesões localizadas, e acentuada intensidade fúngica (44,4%) nos gatos com a forma disseminada da doença e estado geral ruim. Granulomas bem formados (n=4, 30,7%) apresentaram leve a moderada intensidade de carga fúngica, sendo 75% dos animais com esse tipo de granuloma com apenas uma lesão e bom estadogeral. O tratamento de longo prazo com itraconazol, nesses gatos com esporotricose refratária, pode manter a infecção controlada e lesões localizadas estáveis de forma temporária, contudo a reativação do fungo pode ocorrer levando a uma resposta imunológica exuberante e ineficiente.

ABSTRACT: Sporotrichosis is a chronic fungal infection caused by Sporothrix species. The occurrence of cases that are resistant to long-term treatment, especially in the nasal planum of cats, emphasizes the importance of studying its pathogenesis. The purpose of this study was to analyze and compare the inflammatory process of cutaneous lesions of feline refractory sporotrichosis to clinical aspects through cytopathological and histopathological examination. Moreover, the study included 13 cats with cutaneous lesions that had been resistant to itraconazole treatment for more than a year. Cutaneous lesions samples were collected for cytopathological, histopathological, and fungal culture analyses. Tissue fragments were processed and stained with hematoxylin-eosin (HE) and Grocott methenamine silver (GMS). Further, two clinical presentations had the highest occurrence: the localized cutaneous form in animals with good general condition and stable disease (n=9, 69.2%) and the disseminated cutaneous form in cats with poor general condition (n=4, 30.8%). In cats with refractory sporotrichosis, the nasal planum (84.6%) was the most common location of lesions. In the cytopathological study, cats with fewer than two lesions and in good general condition (n=9, 69.2%) showed absence or mild yeast intensity (up to 5 yeasts per field), lower intensity of macrophages and neutrophils, and higher intensity of epithelioid cells, lymphocytes, plasma cells, and eosinophils. On the other hand, (n=4, 30.8%) of the cats with disseminated sporotrichosis and a poor general condition had a marked intensity of yeasts, which were mostly phagocytosed by an increased number of macrophages and neutrophils. Of those animals with good general condition, the majority (n=6, 66.7%) had higher eosinophil intensity. In histopathology, malformed suppurative granuloma was the predominant type (n=9, 69.2%) in feline sporotrichosis lesions, followed by well-formed granulomas (n=4, 30.8%). Malformed granulomas showed mild to moderate fungal intensity (55.6%) in animals with good general condition and localized lesions while marked fungal intensity (44.4%) in cats with the disseminated form of the disease and poor general condition. Well-formed granulomas (n=4, 30.7%) had mild to moderate intensity of fungal load, and 75% of the animals with this type of granuloma had only one lesion and were in good general condition. Long-term itraconazole treatment in these cats with refractory sporotrichosis can keep the infection under control and localized lesions stable; however, fungus reactivation can occur, resulting in an exuberant and inefficient immune response.

RESUMO O trabalho teve por objetivo avaliar a ação do fungicida sítio-específico carbendazim + tebuconazole e do fungicida multissítio clorotalonil, in vitro, sobre a germinação dos uredosporos de Phakopsora pachyrhizi, e in vivo sobre a severidade dos sintomas da doença em folíolos de soja. Os uredosporos de Phakopsora pachyrhizi empregados nos experimentos foram provenientes de Artur Nogueira-SP, coletados a partir de ensaios de campo realizados em três épocas de cultivo, dentro da safra 2016/17. Para cada época de semeadura foram utilizadas três cultivares distintas, obedecendo-se às recomendações segundo o grupo de maturação de cada uma (precoce, médio e tardio), todas suscetíveis à ferrugem asiática, perfazendo um total de 9 cultivares, ou seja, de 9 coletas. Avaliou-se a ação do fungicida multissítio clorotalonil e sítio-específico carbendazim + tebuconazole sobre a germinação in vitro de urediniosporos e a severidade da doença, pelo método de folhas destacadas, in vivo, tratadas preventivamente, antes da inoculação. Determinou-se as concentrações letais CL50 e CL95 pelo método Probit. O fungicida multissítio clorotalonil foi mais fungitóxico in vitro e in vivo que o fungicida específico carbendazim + tebuconazole para os uredosporos de P. pachyrhizi, em todas as coletas referentes, independente das safras e épocas de semeadura. A partir dos testes in vivo e in vitro, há indicação de haver interação significativa dos cultivares com cada fungicida.

ABSTRACT The present study aimed to evaluate the action of both the site-specific fungicide carbendazim + tebuconazole and the multisite fungicide chlorothalonil, in vitro, on the germination of Phakopsora pachyrhizi uredospores and, in vivo, on the severity of symptoms of the disease in soybean leaflets. The uredospores of Phakopsora pachyrhizi used in the experiments were collected from Artur Nogueira, São Paulo State, during field trials carried out in three growing periods within the 2016/17 season. For each sowing period, three different cultivars, susceptible to Asian soybean rust, were used according to the recommendations for their respective maturation group (early, medium and late), totaling 9 cultivars, i.e., 9 samplings. The action of both the multisite fungicide chlorothalonil and the site-specific fungicide carbendazim + tebuconazole was evaluated on the germination of urediniospores, in vitro, and on the disease severity, in vivo, by the method of detached leaves, preventively treated before inoculation. Lethal concentrations LC50 and LC95 were determined by the Probit method. The multisite fungicide chlorothalonil was more fungitoxic in vitro and in vivo than the specific fungicide carbendazim + tebuconazole to P. pachyrhizi uredospores in all samplings, regardless of seasons and sowing times. The in vivo and in vitro tests indicated significant interaction of the cultivars with each fungicide.

Resumo O artigo tem por objetivo monitorar a produção ambulatorial em Odontologia e avaliar a associação de indicadores de ação coletiva e exodontia com o número de Equipes de Saúde Bucal (ESB) entre 2006 e 2015, no Paraná. Neste estudo ecológico longitudinal foram analisados dados secundários consolidados (ações coletivas de escovação supervisionada-ES, aplicação tópica de flúor-ATF, bochecho fluorado-BF, exame bucal com finalidade epidemiológica-EB e exodontia de dente permanente-EXO) do Sistema de Informação Ambulatorial e números de ESB no Sistema de Cadastro Nacional de Estabelecimentos de Saúde. Foram realizadas análises descritivas e correlação de Pearson, com nível de significância de p<0,05. Verificou-se um aumento de ESB implantadas ao longo do tempo e correlação forte positiva com procedimentos coletivos de ES (r=0,78; p=0,007) e BF (r=0,76; p=0,011) e correlação moderada negativa com EXO (r=-0,53). Conclui-se que os indicadores avaliados demonstraram que a implantação de Equipes de Saúde Bucal pode ter contribuído para mudanças no modelo de atenção, com aumento dos procedimentos coletivos preventivos e redução de perdas dentárias no Paraná.

Abstract This article aims to monitor the indicators of outpatient production of Dentistry and to evaluate the association of collective action and extraction indicators with the number of Oral Health Teams (OHT) between 2006 and 2015 in Paraná. In this longitudinal ecological study, consolidated secondary data were analyzed (collective actions of supervised brushing-SB, topical application of fluoride-TAF, fluoride mouthwash-FM, oral examination for epidemiological purposes-OE and extractions of permanent teeth-EX) from the Ambulatory Information System (SIA-SUS) and OHT numbers from the National Registry System of Health Establishments. Descriptive analyzes and Pearson’s correlation were performed, with significance level of p<0.05. It was verified the increase of the OHT implantation over time and a strong positive correlation with collective procedures of SB (r=0.78; p=0.007) and FM (r=0.76; p=0.011) and moderate negative correlation with EX (r=-0.53). It was concluded that the evaluated indicators showed that the implementation of Oral Health Teams may have contributed to changes to the healthcare model, with an increase in preventive collective procedures and reduction of tooth loss in Paraná.

Resumo O artigo tem por objetivo monitorar a produção ambulatorial em Odontologia e avaliar a associação de indicadores de ação coletiva e exodontia com o número de Equipes de Saúde Bucal (ESB) entre 2006 e 2015, no Paraná. Neste estudo ecológico longitudinal foram analisados dados secundários consolidados (ações coletivas de escovação supervisionada-ES, aplicação tópica de flúor-ATF, bochecho fluorado-BF, exame bucal com finalidade epidemiológica-EB e exodontia de dente permanente-EXO) do Sistema de Informação Ambulatorial e números de ESB no Sistema de Cadastro Nacional de Estabelecimentos de Saúde. Foram realizadas análises descritivas e correlação de Pearson, com nível de significância de p<0,05. Verificou-se um aumento de ESB implantadas ao longo do tempo e correlação forte positiva com procedimentos coletivos de ES (r=0,78; p=0,007) e BF (r=0,76; p=0,011) e correlação moderada negativa com EXO (r=-0,53). Conclui-se que os indicadores avaliados demonstraram que a implantação de Equipes de Saúde Bucal pode ter contribuído para mudanças no modelo de atenção, com aumento dos procedimentos coletivos preventivos e redução de perdas dentárias no Paraná.

Abstract This article aims to monitor the indicators of outpatient production of Dentistry and to evaluate the association of collective action and extraction indicators with the number of Oral Health Teams (OHT) between 2006 and 2015 in Paraná. In this longitudinal ecological study, consolidated secondary data were analyzed (collective actions of supervised brushing-SB, topical application of fluoride-TAF, fluoride mouthwash-FM, oral examination for epidemiological purposes-OE and extractions of permanent teeth-EX) from the Ambulatory Information System (SIA-SUS) and OHT numbers from the National Registry System of Health Establishments. Descriptive analyzes and Pearson’s correlation were performed, with significance level of p<0.05. It was verified the increase of the OHT implantation over time and a strong positive correlation with collective procedures of SB (r=0.78; p=0.007) and FM (r=0.76; p=0.011) and moderate negative correlation with EX (r=-0.53). It was concluded that the evaluated indicators showed that the implementation of Oral Health Teams may have contributed to changes to the healthcare model, with an increase in preventive collective procedures and reduction of tooth loss in Paraná.

This work describes the importance of using renewable sources in experimental classes for undergraduate students, as well as to spread the ideas of using biomass wastes as a source of products of interest. Therefore, in the proposed experiment, citrus fruit peel waste was employed to obtain pure flavonoids by extraction and recristalization. These procedures are important to reveal to students how bioactive products can be easily obtained from natural sources. In this way, naringin and hesperidin could be efficiently extracted from grapefruit (Citrus x. paradisi L.) and common orange (Citrus sinensis L. osbeck) peel wastes, respectively, using simple alcohols as solvents.

Resumo O óleo essencial de Melaleuca alternifolia (Maiden & Betche) (óleo da árvore do chá) foi eficaz em estudos anteriores, no tratamento da infestação por ácaros Demodex em humanos. O presente estudo teve por objetivo avaliar o tempo do efeito acaricida, in vitro, desse fitoterápico sobre Demodex canis. Para o exame parasitológico, amostras foram coletadas da pele de um cão e examinadas por microscopia óptica. Apenas amostras com ácaros íntegros e com evidente movimentação de quelíceras e tarsos foram selecionadas. Vinte e uma amostras foram direcionadas para teste com o óleo, em sete concentrações diferentes: 100%; 50%; 25%; 12,5%; 6,25%; 5,0% e 3,13%. Três amostras foram testadas com o controle positivo, amitraz, e três com o controle negativo, xampu neutro. O intervalo entre o momento em que a solução foi adicionada e o instante em que cessou o movimento do último ácaro definiu o tempo de sobrevivência na amostra. Os tempos nas diferentes concentrações e nos controles foram comparados. Quanto mais alta a concentração do óleo, mais rapidamente ocorreu seu efeito letal. Os tempos de sobrevivência nos controles foram maiores que nas diferentes concentrações do óleo de melaleuca.

Abstract The essential oil of the Melaleuca alternifolia (Maiden & Betche) (tea tree oil) has been effective in previous studies, in the treatment of infestation by Demodex mites in humans. The present study aimed at evaluating the in vitro acaricidal effect of this herbal medicine on Demodex canis. For the parasitological examination, samples were collected from a dog’s skin and examined using optical microscopy. Only samples with intact mites and with evident movement of chelicerae and tarsi were selected. Twenty-one samples were tested with the oil, in seven different concentrations: 100%; 50%; 25%; 12.5%; 6.25%; 5.0% and 3.13%. Three samples were tested with the positive control amitraz, and three with the negative control neutral shampoo. The interval between the time the solution was added and the moment the movement of the last mite ceased defined the survival time in the sample. By comparing the times at different concentrations and controls, the results showed that the higher the concentration of the oil, the more quickly its lethal effect occurred, and that the survival times in the controls were longer than in the different concentrations of melaleuca oil.

Resumo: O objetivo deste trabalho foi caracterizar bacteriófagos autóctones e determinar sua atividade lítica em Staphylococcus aureus. Seis fagos foram isolados de água de lavagem de pisos de estábulos por meio do enriquecimento de cultura com três estirpes de S. aureus. Todos os fagos foram caracterizados pela digestão do DNA com enzimas de restrição e pelo sequenciamento do fragmento de DNA que codifica a endolisina. Cada fago foi testado contra 100 estirpes de S. aureus isoladas de casos de mastite bovina e de produtos lácteos pelo método de lise em placa. Sequências do gene de endolisina apresentaram alta conservação, com mais de 99% de similaridade a nível do nucleotídeo entre os fagos isolados. Foram identificados três domínios envolvidos no reconhecimento e na lise da parede celular bacteriana. Dois bacteriófagos isolados de estábulos apresentam alta atividade lítica em S. aureus, em ampla gama de estirpes, o que indica seu potencial para estudos de fagoterapia em gado leiteiro ou como agente de controle biológico para produtos lácteos.

Abstract: The objective of this work was to characterize autochthonous bacteriophages and to determine their lytic activity on Staphylococcus aureus. Six phages were isolated from dairy barn flush water through enrichment cultures with three S. aureus strains. All phages were characterized by DNA digestion by restriction enzymes and sequencing of the DNA fragment encoding endolysin. Each phage was tested against 100 S. aureus strains isolated from bovine mastitis and from dairy products using the lysis-plate method. The sequences of the endolysin gene were highly conserved, with nucleotide similarity higher than 99% among the isolated phages. Three domains involved in the recognition and lysis of the bacterial cell wall were identified. Two bacteriophages isolated from the dairy barns present high lytic activity on S. aureus, on a wide range of host strains, indicating their potential for studies on phage therapy in dairy cattle or as a biological control agent for dairy products.

ABSTRACT Objective To describe the sociodemographic and clinical characterization of patients with chronic hepatitis C followed at the outpatient clinic of a reference hospital in infectology. Method A cross-sectional, descriptive, quantitative study with chronic hepatitis C patients attended at a referral hospital during November 2015 to April 2016 with a sample of 47 users. Results The participants were male (76.6%), 57 years old (57.5%), brown (38.3%), married (55.3%), (61.7%), with a discovery time of up to 6 years (68.1%), not knowing the form of contamination (57.5%), immunized against hepatitis B (65.9%), undergoing drug therapy (85.1%) with Ribavirin (55.6%); And 70.2% had adverse effects. Conclusion Sociodemographic and clinical characterization assist the clinical practice of the multiprofessional team with patients with chronic hepatitis C.

RESUMO Objetivo Descrever a caracterização sociodemográfica e clínica dos pacientes portadores de hepatite C crônica acompanhados no ambulatório de um hospital referência em infectologia. Método Estudo transversal, descritivo, quantitativo, com usuários portadores de hepatite C crônica assistidos no ambulatório de um hospital referência durante Novembro 2015 a Abril de 2016 com uma amostra de 47 usuários. Resultados Os participantes encontram-se no sexo masculino (76,6%) com faixa etária acima de 57 anos (57,5%), pardo (38,3%), casado (55,3%), com grau de escolaridade fundamental incompleto (31,9%), e residente na capital (61,7%), com tempo de descoberta de até 6 anos (68,1%), desconhecendo a forma de contaminação (57,5%), realizando tratamento medicamentoso (85,1%) com Ribavirina (55,6%); e 70,2% apresentaram efeitos adversos. Conclusões A caracterização sociodemográfica e clínica auxiliam na prática clínica da equipe multiprofissional com os portadores de hepatite C crônica.

RESUMEN Objetivo Describir la caracterización sociodemográfica y clínica de los pacientes portadores de hepatitis C crónica acompañados en el ambulatorio de un hospital de referencia en infectología. Método Estudio transversal, descriptivo, cuantitativo, con usuarios portadores de hepatitis C crónica asistidos en el ambulatorio de un hospital de referencia durante noviembre de 2015 a abril de 2016 con una muestra de 47 usuarios. Resultados Los participantes son de sexo masculino (76,6%) con rango de edad superior a 57 años (57,5%), pardo (38,3%), casado (55,3%), con grado de escolaridad (31,9%), y residente en la capital (61,7%), con tiempo de descubrimiento de hasta 6 años (68,1%), desconociendo la forma de contaminación (57,5%), inmunizado contra la hepatitis B (65,9%), realizando tratamiento medicamentoso (85,1%) con Ribavirina (55,6%); Y el 70,2% presentó efectos adversos. Conclusión La caracterización sociodemográfica y clínica auxilian en la práctica clínica del equipo multiprofesional con los portadores de hepatitis C crónica.

Resumo A concepção de Rede de Proteção e Defesa da Pessoa Idosa (RPDI) se destaca como espaço de luta coletiva pelo direito dos idosos, por meio de reuniões sistemáticas destes com profissionais de diferentes instituições e pela articulação para a implantação de políticas públicas. Este estudo, caracterizado como pesquisa qualitativa pautada no método psicanalítico, teve o objetivo de compreender o imaginário coletivo de idosos que participam de encontros organizados pela RPDI sobre o envelhecimento. Os participantes foram convidados para entrevista coletiva, na qual foi utilizado o procedimento de desenhos-estórias com tema como recurso facilitador da comunicação emocional. A análise dos desenhos e narrativas possibilitou reconhecer dois campos de sentido afetivo-emocional denominados “velhice como desamparo” e “velhice como responsabilidade pessoal”. Os caminhos que levaram aos campos de sentido apontaram dinâmicas heterogêneas no imaginário coletivo sobre o envelhecimento, além de contradições inerentes ao ser idoso. Apesar do desamparo, da luta contínua e persistente, das fatalidades e da negação da velhice, está também presente a força que a experiência de vida lhes dá e a motivação que encontram na união com seus pares para enfrentar os desafios da vida e lutar por seus direitos.

Abstract The conception of the Protection and Defense Network of the Older Person (RPDI) stands out as a space of collective struggle for the right of the older people, through systematic meetings between them and professionals from different institutions and by articulation for the implantation of public policy. This study, characterized as qualitative research and based on the psychoanalytic method, aimed at understanding the collective imaginary of the older adults, who attend meetings on aging organized by the RPDI. The RPDI participants were invited to a collective interview, in which the Drawing-Story with Theme Procedure was used as a resource for facilitating emotional communication. The analysis of these drawings and narratives made it possible to recognize two affective-emotional meaning fields, named “Old age as helplessness” and “Old age as personal responsibility”. The paths that led to the fields of meaning indicated heterogeneous dynamics in the collective imaginary about aging, and contradictions in aging have been identified. Despite the helplessness, continuous and persistent struggle, fatalities, and the denial of old age, there is also the strength that the experience of life gives them and the motivation they find in union with their pairs to face the challenges of life and fight for their rights.

Resumo A concepção de Rede de Proteção e Defesa da Pessoa Idosa (RPDI) se destaca como espaço de luta coletiva pelo direito dos idosos, por meio de reuniões sistemáticas destes com profissionais de diferentes instituições e pela articulação para a implantação de políticas públicas. Este estudo, caracterizado como pesquisa qualitativa pautada no método psicanalítico, teve o objetivo de compreender o imaginário coletivo de idosos que participam de encontros organizados pela RPDI sobre o envelhecimento. Os participantes foram convidados para entrevista coletiva, na qual foi utilizado o procedimento de desenhos-estórias com tema como recurso facilitador da comunicação emocional. A análise dos desenhos e narrativas possibilitou reconhecer dois campos de sentido afetivo-emocional denominados “velhice como desamparo” e “velhice como responsabilidade pessoal”. Os caminhos que levaram aos campos de sentido apontaram dinâmicas heterogêneas no imaginário coletivo sobre o envelhecimento, além de contradições inerentes ao ser idoso. Apesar do desamparo, da luta contínua e persistente, das fatalidades e da negação da velhice, está também presente a força que a experiência de vida lhes dá e a motivação que encontram na união com seus pares para enfrentar os desafios da vida e lutar por seus direitos.

Abstract The conception of the Protection and Defense Network of the Older Person (RPDI) stands out as a space of collective struggle for the right of the older people, through systematic meetings between them and professionals from different institutions and by articulation for the implantation of public policy. This study, characterized as qualitative research and based on the psychoanalytic method, aimed at understanding the collective imaginary of the older adults, who attend meetings on aging organized by the RPDI. The RPDI participants were invited to a collective interview, in which the Drawing-Story with Theme Procedure was used as a resource for facilitating emotional communication. The analysis of these drawings and narratives made it possible to recognize two affective-emotional meaning fields, named “Old age as helplessness” and “Old age as personal responsibility”. The paths that led to the fields of meaning indicated heterogeneous dynamics in the collective imaginary about aging, and contradictions in aging have been identified. Despite the helplessness, continuous and persistent struggle, fatalities, and the denial of old age, there is also the strength that the experience of life gives them and the motivation they find in union with their pairs to face the challenges of life and fight for their rights.