Abstract Objectives: To screen the COL1A1 and COL1A2 gene mutation sites in a family with type I osteogenesis imperfecta (OI)/hearing loss and analyze the characteristics and recovery of hearing loss in patients with osteogenesis imperfecta. Methods: The basic clinical data of Ol proband and her parents were collected, and the COL1A1 and COL1A2 genes were detected in peripheral blood by PCR amplification and generation Sanger sequencing. Literature of stapedial surgery in patients with osteogenesis imperfecta was collected. Results: The heterozygous mutation of the 26 exon c.1922_1923 ins C in the Ol progenitor COL1A1 gene led to the amino acid frameshift mutation of p.Pro 601FS, which was not detected in the phenotypic parents. The homozygous of exon 28 c.1782>G in COL1A2 was detected in the proband and her parents, resulting in changes in the protein p.Pro 549Ala. Conclusion: The clinical symptoms of the Ol proband is caused by heterozygous mutation of the 26 exon c.1922_1923 ins C in COL1A1 gene. Stapedial surgery can provide short-term and long-term hearing benefits for Ol patients with hearing loss. Level of evidence: Level 4. Objectives COLA COL A COL1A OI/hearing OIhearing OI /hearing Methods collected sequencing Results 2 c19221923 c 1922 1923 c.1922_192 pPro p Pro 601FS FS c1782G cG 1782 G 549Ala Ala Conclusion shortterm short term longterm long evidence 4 c1922192 192 c.1922_19 178 c192219 19 c.1922_1 17 c19221 1 c.1922_ c1922 c.1922 c192 c.192 c19 c.19 c1 c.1 c.

RESUMEN El método eléctrico de corriente continua (DC) se usa frecuentemente para la detección avanzada de cuerpos geológicos anormales ricos en agua y ocultos en la calzada de la mina y así asegurar la operación extractiva de carbón. Sin embargo, en cierto alcance, la exactitud de la detección puede estar alterada por la cavidad de la calzada, lo que significa una desviación en la ubicación de la anomalía. Para investigar este fenómeno se analizó el principio de detección avanzada, que está basado en el modelo físico esférico, a través de métodos de "análisis comparativos" y "simulados" a través del software Cosmol Multiphysics. De acuerdo con los principios de simulaciones numéricas, se introdujo la influencia de la cavidad de la calzada en la exactitud de la detección de corriente continua avanzada y se computó la proporción de resistividad aparente calculada con este método frente a aquella calculada solo con información de la cavidad de la calzada para así obtener el coeficiente de corrección. Este coeficiente se empleó para corregir la información de resistividad aparente en la detección 3-D avanzada. Adicionalmente, se discutieron las características de respuesta de las anomalías en varias posiciones con un diseño de electrodos en la calzada. Los resultados muestran que el método de proporción corrige efectivamente la influencia de la calzada durante el avance de la detección a través del método eléctrico de corriente continua y simultáneamente mejora la visualización de las anomalías geológicas y la exactitud del posicionamiento de estas anomalías. Además, la influencia de la calzada se ve reflejada en las posiciones relativas de los electrodos a lo largo de la calzada. Cuando los electrodos están en la zona donde confluyen el suelo y la pared, la curva de resistividad obtenida se ve menos menos afectada. Por lo tanto, la resolución de las anomalías es mayor y sus posiciones son más exactas. Además, una aplicación práctica del método de proporción indicó que las curvas corregidas reflejaron mejor las anomalías geológicas.

ABSTRACT To ensure safety during coal mining, the DC electric method is frequently used for the advanced detection of concealed water-rich abnormal geological bodies located ahead of a drivage roadway. However, to some extent, the accuracy of the detection is influenced by the roadway cavity, resulting in a deviation from the actual location of the anomaly. To investigate this phenomenon, the principle of advanced detection based on a spheroidal physical model was analyzed via "comparative analyses" and "simulated" methods using COSMOL Multiphysics software. Following the principle of numerical simulations, the influence of the roadway cavity on the accuracy of the advanced DC detection method was introduced, and the ratio of apparent resistivity calculated from actual data to that calculated only with the roadway cavity was carried out to obtain the roadway correction coefficient. This coefficient was employed to correct the apparent resistivity data for advanced 3-D detection. Additionally, the response characteristics of the anomalies at different electrode layout locations in the roadway were discussed. The results show that the ratio method effectively corrects the influence of the roadway during advance detection using the DC electric method and simultaneously improves the resolution of geological anomalies and the accuracy of positioning. Additionally, the influence of the roadway was related to the relative positions of the electrodes along the roadway. When the electrodes are set at the junction of the floor and the sidewall, the obtained resistivity curve is less affected. Therefore, the resolutions of the anomalies are higher, and their positioning is more accurate. Furthermore, a practical application of the ratio method indicated that the corrected curves reflected the geological anomalies better.

A new spectrophotometric method was developed for the determination of taurine using 7,7,8,8-tetracyanoquinodimethane (TCNQ). The method was based on the formation of charge transfer (CT) complex of this drug as n-electron donor with the π-acceptor TCNQ. TCNQ was found to react with taurine to produce a kind of yellow complex. The spectrum obtained for taurine/TCNQ system showed the maximum absorption band at a wavelength of 420 nm. The formation of such complex was also confirmed by both infrared and 1H NMR measurements. Different variables affecting the reaction were studies and optimized. Beer’s law was obeyed in a concentration limit of 1~9 µg mL-1 for taurine. R.S.D. was less than 1.5%, and average recovery was over 100.3%. The stoichiometry of the complex was found to be 1:1 ratio by Job’s and straight line methods between the donor and the acceptor. The thermodynamic standard reaction quantities of the complex between the donor and the acceptor were estimated. The developed method could be applied successfully for the determination of the studied compound in the pharmaceutical dosage form with a good precision and accuracy compared to official and reported method as revealed by t- and F-tests.

Abstract This study aimed to investigate the potential of a novel preparation strategy for glycosylated hydrolysate by liquid fermentation in order to provide a high-quality glycosylated hydrolysate. Protein solubility, surface hydrophobicity and antioxidant activity were evaluated. The results of Fourier transform infrared spectra demonstrated the occurrence of glycosylation reaction. The results of Fluorescence emission spectroscope revealed the decrease of tertiary conformation stability. Circular dichroism spectra revealed significant decrease in α-helix and increase in β-sheet and random coil by glycosylation reaction. In comparison with zein hydrolysate, glycosylated hydrolysate had lower surface hydrophobicity but higher in solubility and in vitro digestibility due to glycosylation. Glycosylated hydrolysate presented higher ABTS (2,2'-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt) and superoxide radicals scavenging activities as well as Cu2+ chelating capacity. It is concluded that the glycosylation confer an open structure and modified properties of zein, and glycosylated hydrolysate is a potential ingredient with better hydration and antioxidant activities than zein hydrolysate.

ABSTRACT In this study, our goal was to clarify the role of proteasomal subunit α-1 (PSMA1) in both the differentiation of preadipocytes and the accumulation of lipids in adipocytes. Preadipocytes from healthy one-day-old calves were collected, isolated, and cultured in vitro. The expression pattern of the PSMA1 gene was explored during the differentiation of bovine preadipocytes firstly. Then, the expression of the PSMA1 gene was inhibited by transfection of a chemically synthesized small interfering RNA (siRNA) before differentiation. After induction of differentiation, the mRNA levels of key regulating genes involved in preadipocyte differentiation and the lipid content of mature adipocytes with and without inhibition of PSMA1 were detected by qRT-PCR and oil red O staining, respectively. The data showed that PSMA1 mRNA was differentially expressed during the differentiation of bovine preadipocytes under normal culture conditions in vitro. The expression level of peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT enhancer-binding protein alpha (C/EBPα), and lipoprotein lipase (LPL) were significantly decreased in the transfected PSMA1-siRNA group compared with those in the control group, and the mRNA levels of the preadipocyte factor-1 (Pref-1) were significantly upregulated in the transfected PSMA1-siRNA group compared with those in the control group. In addition, significantly fewer lipid droplets were formed by adipocytes transfected with PSMA1-siRNA than by the negative control group (adipocytes transfected with NC-siRNA). Therefore, PSMA1 plays an important role in differentiation and lipid deposition.