ABSTRACT Returning agricultural waste/by-products to the field is an effective management measure to improve soil fertility and maintain crop productivity in agroecosystems. However, we still have limited understanding of the complex response of sisal (Agave sisalana Perrine) growth, soil nutrients and microbial communities to the long-term sisal residue return to the ecological system. This study aimed to investigate the comprehensive effects of returning sisal residue to the field on sisal yield, soil nutrient, and microbial community. Based on a four-year field experiment, the results showed that returning sisal residue to the field increased the annual yield of sisal by 30.77–33.36%. Compared with control group, sisal residue inputs significantly improved soil total nitrogen, available phosphorus, and available potassium by 46.59, 3.02, and 1.21%, respectively. Under sisal residue input condition, the activities of soil catalase, urease, acid phosphatase, and sucrase also significantly increased by 2.01, 17.20, 17.40 and 16.60%, respectively. A higher ratio of bacteria/fungi was observed in the soil amended with sisal residue. The microbial diversity analysis showed that the α-diversity of the bacterial community increased, while the α-diversity of the fungal community decreased with the sisal residue treatment. However, sisal residue return had a higher impact on the α-diversity of fungi. This study provides evidence that returning sisal residue to the field affects sisal productivity by regulating nutrient cycling and soil microbial community. Moreover, the study suggests that microbial α-diversity has significant influence on sisal yield. waste/byproducts wastebyproducts waste/by products waste agroecosystems However Agave Perrine growth longterm long term system fouryear four year experiment 30773336 30 77 33 36 30.77–33.36% group nitrogen phosphorus 4659 46 59 46.59 302 3 02 3.02 121 1 21 1.21% respectively condition catalase urease phosphatase 201 2 01 2.01 1720 17 20 17.20 1740 40 17.4 1660 16 60 16.60% bacteriafungi bacteria fungi αdiversity α treatment Moreover byproducts wasteby 3077333 7 30.77–33.36 465 4 5 46.5 0 3.0 12 1.21 2.0 172 17.2 174 17. 166 6 16.60 307733 30.77–33.3 46. 3. 1.2 2. 16.6 30773 30.77–33. 1. 16. 3077 30.77–33 307 30.77–3 30.77– 30.77 30.7 30.

Abstract The purple glume-tip is an essential morphological marker for selective rice breeding, aiding in assisted selection and variety purification. However, the inheritance of purple glume-tip in japonica rice landrace Donglan Black Rice (DBR) has not yet been explored deeply. The F2 and F4 populations were constructed from crossing between Huazhan with colorless glume-tip and DBR to identify the associated genomic region(s). Genetic analysis displayed two highly comparable and perplexing phenotypes in the purple glume-tip of rice. Two significant genes with recessive epistasis predominantly regulated the two phenotypes. The two target gene loci were located in the intervals of 5315163-5316875 bp on chr6 and 27915598-27939357 bp on chr4, respectively, where reported genes associated with the purple color trait in rice, Os06g0205100 and Os04g0557500, are present. The two genes may be potential target genes. However, the role of Os04g0557500 in the glume-tip coloration remains unreported. glumetip glume tip breeding purification However (DBR deeply F regions. regions region s . region(s) 53151635316875 5315163 5316875 5315163-531687 chr 2791559827939357 27915598 27939357 27915598-2793935 chr4 respectively Osg Os g Os06g020510 present Os04g055750 unreported region(s 5315163531687 531516 531687 5315163-53168 279155982793935 2791559 2793935 27915598-279393 Os06g02051 Os04g05575 531516353168 53151 53168 5315163-5316 27915598279393 279155 279393 27915598-27939 Os06g0205 Os04g0557 53151635316 5315 5316 5315163-531 2791559827939 27915 27939 27915598-2793 Os06g020 Os04g055 5315163531 531 5315163-53 279155982793 2791 2793 27915598-279 Os06g02 Os04g05 531516353 53 5315163-5 27915598279 279 27915598-27 Os06g0 Os04g0 53151635 5 5315163- 2791559827 27 27915598-2 Os06g Os04g 279155982 2 27915598-

BACKGROUND: Hepcidin, encoding by HAMP gene, is the pivotal regulator of iron metabolism, controlling the systemic absorption and transportation of irons from intracellular stores. Abnormal levels of HAMP expression alter plasma iron parameters and lead to iron metabolism disorders. Therefore,itis animportant goal to understand the mechanisms controlling HAMP gene expression. RESULTS: Overexpression of Sox2 decrease basal expression of HAMP or induced by IL-6 or BMP-2, whereas, knockdown of Sox2 can increase HAMP expression, furthermore, two potential Sox2-binding sites were identified within the human HAMP promoter. Indeed, luciferase experiments demonstrated that deletion of any Sox2-binding site impaired the negative regulation of Sox2 on HAMP promoter transcriptional activity in basal conditions. ChIP experiments showed that Sox2 could directly bind to these sites. Finally, we verified the role of Sox2 to negatively regulate HAMP expression in human primary hepatocytes. CONCLUSION: We found that Sox2 as a novel factor to bind with HAMP promoter to negatively regulate HAMP expression, which may be further implicated as a therapeutic option for the amelioration of HAMP-overexpression-related diseases, including iron deficiency anemia.

Nanotubos de carbono de multi-paredes oxidados (ox-MWCNT) foram utilizados como condutores adicionais para preparar uma nova rede de cátodos para bateria de íons de lítio. A morfologia foi analisada pelo microscópio de varredura eletrônico, e partículas ativas de LiMn2O4 foram ligados ao ox-MWCNT com a formação da rede tridimensional de fiação. O transporte de elétrons e a atividade eletroquímica foram melhorados de forma eficaz. Testes galvanostáticos de carga-descarga do cátodo LiMn2O4/ox-MWCNT mostrou que a capacidade de descarga inicial são 119,4, 110,6, 105,5 e 91,4 mAh g-1 à taxa de 0,1, 0,5, 1 e 2 C, respectivamente, os quais são muito superiores aos de LiMn2O4/acetileno (AB), de mesmo conteúdo. A espectroscopia da impedância eletroquímica AC mostrou que a resistência da transferência de carga (Rct) do eletrodo composto reduziu de 34,32 Ω para LiMn2O4/ox-MWCNT, em comparação ao valor de 53,2 Ω para LiMn2O4/AB. De um modo geral, se verificou que uma rede condutora para facilitar a transferência de elétrons e fazer uma boa conexão das partícula ativas com o material da rede desempenham um papel importante para avaliar a capacidade e eficiência do ciclo.

Oxidized multi-walled carbon nanotubes (ox-MWCNT) were used as conducting addition to prepare a novel network composite cathode for lithium ion battery. The morphology was analyzed by scanning electron microscope, LiMn2O4 active particles were connected by ox-MWCNT with the formation of three-dimensional networking wiring. Electron transport and electrochemical activity were improved effectively. Galvanostatic charge-discharge tests of LiMn2O4/ox-MWCNT cathode showed that the initial discharge capacities are 119.4, 110.6, 105.5 and 91.4 mAh g-1 at the rate of 0.1, 0.5, 1 and 2 C, respectively, which were much higher than LiMn2O4/acetylene black (AB) at the same content. The electrochemical AC impedance spectroscopy showed that the charge transfer resistance (Rct) of the composite electrode reduced obviously contrasting to 34.32 Ω for LiMn2O4/ox-MWCNT and 53.2 Ω for LiMn2O4/AB. Overall, it is found that a conductive network to facilitate electron transfer and good connection of the active-material particle to the network were playing an important role to rate capability and cycle efficiency.