PURPOSE:To demonstrate an experimental model of up to four hours a week of independent study that allows relearning in microvascular sutures.METHODS:Wistar rats between 200 and 500 grams surplus research experiments were used. Femoral vessels are covered on one or both sides through a groin incision obliquely along the inguinal ligament. Femoral artery and vein are isolated and measured being clamped and cut. The individual performs in microvascular anastomosis complexity arterial and venous terminoterminal sequence. terminolateral and venous and arterial grafts in vessels. Permeability is evaluated by testing vascular patency after creation of microvascular anastomosis.RESULTS:In the first specimen, only arterial and venous vascular anastomosis are performed terminoterminal. The average diameter of the femoral veins varies from 0.8 to 2 mm between rodents (artery, between 0.6 and 1.4 mm, between 0.8 and 2 mm vein). The superficiality of the vessels allows faster dissection, may also be held in other inguinal region.CONCLUSION:The model of individual retraining allows learning microvascular suture in individuals of permanent staff.

PURPOSE:To present an animal model to assess the effects of end-to-side innervation in the heterotopically transplanted model with reduced chances of neural contamination.METHODS: The medial portion of the gastrocnemius muscle in wistar male rats was isolated and its pedicle dissected and performed a flap in the abdominal portion. To prevent neural contamination in the abdominal region, the muscle was wrapped with a Goretex(r) sheet. The specimens were divided into 2 groups (G). In G1 was performed an end-to-end suture between tibial nerve of the gastrocnemius and femoral motor nerve and between the saphenous sensory nerve and the motor nerve. In G2 was performed a end-to-side suture between the tibial nerve and the motor femoral and between the tibial nerve and saphenous motor nerve. The specimens were evaluated 60 days later to check the structure of the neurorraphy. Sections were obtained proximal and distal to the coaptation site.RESULTS: The medial gastrocnemius muscle had the advantage of maintaining visible mass after 60 days. No disruption of the coaptation site was found. No major injury to the donor nerve was seen in group 2.CONCLUSION:The proposed model is simple, reproduciple and prevent the neural contamination in the flap in end-to-side suture.

OBJETIVO: Através de um modelo experimental, pretendemos criar inferências sobre a viabilidade do enxerto ósseo vascularizado da crista ilíaca em ratos e verificar suas características histológicas. MÉTODO: Foram utilizados 21 ratos, que foram divididos em dois grupos: o primeiro consistindo por animais submetidos à técnica de enxerto ósseo vascularizado e pediculado sobre o ramo ilíaco da artéria iliolombar; o segundo (grupo controle) sofreu o mesmo procedimento do primeiro com a adição da ligadura do pedículo vascular. A viabilidade dos enxertos ósseos foi verificada durante três semanas, através da visualização direta do enxerto, histologia e imunoistoquímica. RESULTADOS: Todos os enxertos vascularizados avaliados na primeira semana apresentaram viabilidade segundo a observação direta, histologia e imunoistoquímica. Entretanto, na segunda e terceira semanas os enxertos mostraram-se inviáveis em 75% dos casos quando submetidos à avaliação segundo a observação direta e em 50% dos casos quando realizada a análise histológica e imunoistoquímica. CONCLUSÃO: Alguns enxertos vascularizados em sua concepção tornaram-se inviáveis e passaram a se comportar como enxertos não vascularizados sob a análise da observação direta e histológica. Apesar da possibilidade de falha, o uso de enxertos ósseos vascularizados deve ser incentivado, pois a histologia descritiva demonstrou maior densidade celular na porção óssea medular, osteócitos com maior funcionalidade na deposição de matriz óssea, com rede vascular intraóssea preservada.

OBJECTIVE: Through an experimental model, our aim was to create inferences about the viability of vascularized bone grafts from the iliac crest in rats and investigate their histological features. METHODS: Twenty-one rats were used, divided into two groups: the first consisted of animals that were subjected to the technique of vascularized bone graft pedicled onto the iliac branch of the iliolumbar artery; the second (control group) underwent the same procedure as performed on the first group, with the addition of ligation of the vascular pedicle. The viability of the bone grafts was observed for three weeks, by means of direct observation of the graft, histology and immunohistochemistry. RESULTS: All the vascularized grafts evaluated in the first week showed viability according to direct observation, histology and immunohistochemistry. However, in the second and third weeks, direct observation showed that 75% of the grafts were unviable, while histological analysis and immunohistochemistry showed that 50% were unviable. CONCLUSIONS: Some grafts that are designed to be vascularized became unviable and began to behave like nonvascularized grafts under direct observation and histology. Despite the possibility of failure, use of vascularized bone grafts should be encouraged, because descriptive histology shows greater cell density in the medullary bone portion, and osteocytes that function better regarding deposition of bone matrix, with preservation of the intraosseous vascular network.