Resumo Fatores de transcrição (FT) são uma ampla classe de genes em plantas e podem regular a expressão de outros genes em resposta a vários estresses ambientais (estresses bióticos e abióticos). No presente estudo, a atividade do fator de transcrição na cana-de-açúcar foi examinada durante o estresse pelo frio. Inicialmente, as leituras de transcrição de RNA de duas cultivares de cana-de-açúcar (ROC22 e GT08-1108) sob estresse frio foram baixadas do banco de dados SRA NCBI. As leituras foram alinhadas em um genoma de referência e as análises de expressão diferencial foram realizadas com o pacote R / Bioconductor edgeR. Com base em nossas análises no cultivar ROC22, 963 genes TF foram significativamente regulados positivamente sob estresse pelo frio entre um total de 5.649 genes regulados positivamente, enquanto 293 genes TF foram regulados negativamente entre um total de 3.289 genes regulados negativamente. No cultivar GT08-1108, 974 genes TF foram identificados entre 5.649 genes regulados positivamente e 283 genes TF foram encontrados entre 3.289 genes regulados negativamente. Os fatores de transcrição, em sua maioria, foram anotados com categorias GO relacionadas à ligação de proteína, ligação de fator de transcrição, ligação específica de sequência de DNA, complexo de fator de transcrição, atividade de fator de transcrição em RNA polimerase II, atividade de fator de transcrição de ligação de ácido nucleico, atividade de corepressor de transcrição, sequência específica da região reguladora, atividade do fator de transcrição da RNA polimerase II, atividade do cofator do fator de transcrição, atividade do fator de transcrição do promotor do plastídio, atividade do fator de transcrição do promotor da RNA polimerase I, polimerase II e RNA polimerase III. As descobertas dos resultados acima ajudarão a identificar fatores de transcrição expressos diferencialmente durante o estresse pelo frio. Ele também fornece uma análise abrangente da regulação da atividade de transcrição de muitos genes. Portanto, este estudo fornece base molecular para melhorar a tolerância ao frio em cana-de-açúcar e outras gramíneas economicamente importantes.

Abstract Transcription factors (TF) are a wide class of genes in plants, and these can regulate the expression of other genes in response to various environmental stresses (biotic and abiotic). In the current study, transcription factor activity in sugarcane was examined during cold stress. Initially, RNA transcript reads of two sugarcane cultivars (ROC22 and GT08-1108) under cold stress were downloaded from SRA NCBI database. The reads were aligned into a reference genome and the differential expression analyses were performed with the R/Bioconductor edgeR package. Based on our analyses in the ROC22 cultivar, 963 TF genes were significantly upregulated under cold stress among a total of 5649 upregulated genes, while 293 TF genes were downregulated among a total of 3,289 downregulated genes. In the GT08-1108 cultivar, 974 TF genes were identified among 5,649 upregulated genes and 283 TF genes were found among 3,289 downregulated genes. Most transcription factors were annotated with GO categories related to protein binding, transcription factor binding, DNA-sequence-specific binding, transcription factor complex, transcription factor activity in RNA polymerase II, the activity of nucleic acid binding transcription factor, transcription corepressor activity, sequence-specific regulatory region, the activity of transcription factor of RNA polymerase II, transcription factor cofactor activity, transcription factor activity from plastid promoter, transcription factor activity from RNA polymerase I promoter, polymerase II and RNA polymerase III. The findings of above results will help to identify differentially expressed transcription factors during cold stress. It also provides a comprehensive analysis of the regulation of the transcription activity of many genes. Therefore, this study provides the molecular basis for improving cold tolerance in sugarcane and other economically important grasses.

Resumen Una investigación relevante y no reportada previamente respecto a la influencia del carbón activado (CA) apoya que la biomasa de Eucalyptus Lanceolata es un adsorbente. El reciente trabajo sintetizó una serie de estudios para conocer diversos parámetros experimentales de tiempo de equilibrio y temperatura del adsorbente para la eliminación del colorante. La caracterización de la estructura física determinó que el aumento del área superficial del carbón activado de 399 a 475 m2.g-1 revela que la biomasa cruda tenía una superficie más compacta que las del carbón activado. Se encontraron bandas FTIR en la región entre 3406.29 cm-1 y 3414.0 cm-1 indicando el estiramiento del grupo (-OH) que conduce al enlace de hidrógeno. Se estudió la adsorción de azul de metileno en carbón crudo y activado. La energía de activación media del carbón activado fue de 33,67 kJ.mol-1, ligeramente inferior a la del carbón bruto, que fue de 49,14 kJ.mol-1. Se aplicaron modelos de difusión intrapartícula, Elovich y Bhangam para evaluar estudios cinéticos a 20, 30 y 40 ºC. Los parámetros termodinámicos, ΔH≠, ΔS≠ y ΔE≠ se calcularon a partir de la cinética de adsorción. Los resultados indican que la adsorción es un proceso espontáneo. El valor negativo de la entropía determinó que las moléculas de ácido toman una posición orientada en la superficie del adsorbente. Los resultados muestran que todos los modelos se ajustaron mejor para la adsorción.

Abstract Investigation relevant to the influence of activated carbon (AC) supports biomass of Eucalyptus Lanceolata as an adsorbent was not reported previously. The recent work synthesized a series of studies to know various experimental parameters equilibration time and temperature of the adsorbent for the elimination of dye. Physical structure characterization measured that the surface area of activated carbon increased from 399 to 475 m2.g-1 revealed that the raw biomass had more compact surface than those of activated carbon. FTIR bands were found in the region between 3406.29 cm-1 and 3414.0 cm -1 indicates (-OH) stretching group which leads to hydrogen bonding. Adsorption of Methylene blue was studied on raw and activated carbon. The average activation energy of the activated carbon was 33.67 kJ.mol-1, slightly lower than those of raw carbon, which was 49.14 kJ.mol-1. Intraparticle diffusion, Elovich and Bhangam models were applied to evaluate kinetics studies at 20, 30 and 40 ºC. Thermodynamic parameters, ΔH ≠, ΔS≠ and ΔE≠ were calculated from the adsorption of kinetics. Results indicate that adsorption is a spontaneous process. The negative value of entropy determined that the acid molecules take an oriented position on the surface of the adsorbent. The results show that all the models were best fitted for adsorption.

Abstract Cashmirins A (1) and B (2), new prenylated coumarins, have been isolated from the EtOAc- soluble fraction of the whole plant of Sorbus cashmiriana Hedlung, Monog. along with seselin (3), scopoletin (4), 3-hydroxyxanthyletin (5) and luteolin (6), reported for the first time from this species. Their structures were elucidated by spectroscopic techniques including MS, 1D and 2D NMR spectroscopy. Both new compounds 1 and 2 were investigated for biological activities and showed significant antifungal and urease inhibitory activities. Compounds 1 and 2 exhibited significant activity against Aspergillus flavus, Macrophomina phaseolina, Trichophyton simii, Trichophyton schoenleinii, and Pseudallescheria boydri. Both compounds also exhibited significant inhibitory activity against Jack bean urease with IC50 values of 28.2±0.12 µM and 30.3±0.18 µM, respectively compared to thiourea used as positive control.

Schiff bases, 2-{[(2-hydroxyphenyl)methylidene]amino}phenol (4) and 2-{[3-4-(dimethylamino)phenyl-2-propenylidene]amino}phenol (5), have been synthesized by the condensation of 2-aminophenol (1) with 2-hydroxybenzaldehyde (2) and 4-dimethylaminocinnamaldehyde (3), respectively. They showed good lipoxygenase inhibition and antioxidant activities, as compound 4 showed potent antioxidant activity. The products also showed good activities against Gram-positive: S. intermedius, B. subtilis and S. aureus, and Gram-negative: E. coli and S. typhi bacteria, as compounds 5 and 4 are excellent active against B. subtilis and S. typhi, respectively. These good to potent activities may be due to the presence of free hydroxyl and amino groups. They showed non-significant urease inhibition activity.