Abstract Background Fibrous scars frequently form at the sites of bone nonunion when attempts to repair bone fractures have failed. However, the detailed mechanism by which fibroblasts, which are the main components of fibrous scars, impede osteogenesis remains largely unknown. Results In this study, we found that fibroblasts compete with osteogenesis in both human bone nonunion tissues and BMP2-induced ectopic osteogenesis in a mouse model. Fibroblasts could inhibit the osteoblastic differentiation of mesenchymal stem cells (MSCs) via direct and indirect cell competition. During this process, fibroblasts modulated the nuclear-cytoplasmic shuttling of YAP in MSCs. Knocking down YAP could inhibit osteoblast differentiation of MSCs, while overexpression of nuclear-localized YAP-5SA could reverse the inhibition of osteoblast differentiation of MSCs caused by fibroblasts. Furthermore, fibroblasts secreted DKK1, which further inhibited the formation of calcium nodules during the late stage of osteogenesis but did not affect the early stage of osteogenesis. Thus, fibroblasts could inhibit osteogenesis by regulating YAP localization in MSCs and secreting DKK1. Conclusions Our research revealed that fibroblasts could modulate the nuclear-cytoplasmic shuttling of YAP in MSCs, thereby inhibiting their osteoblast differentiation. Fibroblasts could also secrete DKK1, which inhibited calcium nodule formation at the late stage of osteogenesis. failed However unknown study BMP2induced BMPinduced BMP2 induced BMP model (MSCs competition process nuclearcytoplasmic nuclear cytoplasmic nuclearlocalized localized YAP5SA YAPSA 5SA SA Furthermore DKK1 DKK Thus

ABSTRACT The objective of this study was to identify differentially expressed genes (DEG) between females and males in early bovine embryos. Superovulated cattle (n = 4) in the age span of 16–18 months were artificially inseminated with semen from the same bull that has been proven to be fertile. Blastocysts were collected by routine non-surgical uterine flushing on day 7 after insemination. This study determines the sex of embryos using a micro-injection pipette to collect a few blastomeres through the Zona pellucida. The remaining blastomeres were used for single-cell RNA sequencing (scRNA-seq) analysis on the Illumina platform, followed by differential expression analysis, Gene Ontology (GO) function analysis, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. The criteria for identifying DEG will be set at |log2(Fold change)| ≥ 1 and P<0.05. A total of 8004 DEG were detected in female (Denoted as BLX, n = 3) and male (Denoted as BLY, n = 3) blastocysts. Transcripts highly expressed in the female embryos were related to catalytic activity, nucleotide binding, and catabolic process, while transcripts highly expressed in the male embryos were linked to oxidative phosphorylation, mitochondrion, and the ribosome. Nine genes that may be involved in blastocyst growth and development were screened, suggesting that their differential expression may be responsible for the differences in the development of male and female embryos. This study provides important information on potential genes and pathways associated with differences in early female and male embryonic development.

Abstract Purpose This study aims to validate the money management intention screening questionnaire under the framework of theory of planned behavior, which includes attitude, subjective norms, perceived behavioral control and intention. Design/methodology/approach A total of 919 undergraduate students with loans were randomly selected and grouped into four sub-studies to address the psychometric properties of the imposed structure. The item–object congruence, confirmatory factor analysis (CFA), test–retest reliability method and other statistical tests were carried out for item selection and confirmation. Two self-reported measures, namely, Saving Behavior Scale and Short Dark Triad (SD3-Thai version), were applied for the measure concurrent validation. Findings The final 12 items with four-component structures were deemed reliable and generally valid in university students with loans, with CFA results indicating good fit indices (χ2= 96.44, df = 43; CFI = 0.96; GFI = 0.94; RMSEA = 0.06). The test–retest method indicated values between 0.66 (subjective norm) and 0.71 (attitude). Machiavellianism from SD3-TH and saving attitude from the Saving Behavior Scale showed the strongest significant relation among the items. The abbreviation of the 12-item structure was labeled in the Money Management Intention Questionnaire (MMIQ-TPB). Research limitations/implications This study provided a reliable and valid substantial structure for identifying money management intention. However, there was a consideration that MMIQ-TPB questions referred to cognitive influences through intention; thus, it was designed to cover the intended preparation and not in the action stage. Practical implications Great money management practically predicts a lower likelihood of being in debt. Attentive educators or loan providers can thus benefit from this alternative structure as a screening scale for identifying risky cognitive mismanagement. Social implications The evidence provided in this study highlights the possibility of identifying students who necessarily need a program to improve their monetary management skills during their studying periods. Policymakers could address this problem at the first stage of the general mode in the loan providing operation. Originality/value This study bridges the gap in the literature on financial behavioral changes for establishing money management intention among undergraduate students with loans. Furthermore, it confirms the advantages and disadvantages of having certain dark personality traits in a financial context.

Tuberculosis (TB) services in China are provided through a large network of TB dispensaries. Even though hospitals are not as well placed to follow recommended standards of TB care, a significant proportion of people with TB symptoms seek care from hospitals. In spite of having a policy and mandate in place, the Ministry of Health had little success in encouraging hospitals to refer suspected TB cases to dispensaries. Following the epidemic of severe acute respiratory syndrome in 2003, the government set up a nationwide Internet-based communicable diseases reporting system. This achieved productive collaboration between hospitals and TB dispensaries. From 2004 to 2007, the percentage of TB suspects and patients needing referral from hospitals who arrived in TB dispensaries increased substantially from 58.7% to 77.8% and the contribution of hospitals to diagnosing sputum smear-positive TB cases doubled from 16.3% to 32.9%. Using the Internet-based reporting system, hospitals in China contributed to finding about one third of all sputu m smear-positive TB cases and helped meet the global TB control target of detecting 70% of such cases. Based on the data available from routine surveillance facilitated by this Internet-based system, this paper details the process and outcomes of strengthening collaboration between hospitals and TB dispensaries using the Internet as a tool and its potential application to other country settings.

Los servicios de atención sanitaria de la tuberculosis (TB) en China se proporcionan a través de una amplia red de dispensarios especializados. Aunque los hospitales no están incluidos en las normas asistenciales recomendadas para la tuberculosis, una proporción considerable de personas con síntomas de tuberculosis acuden a ellos. A pesar de haber dispuesto una normativa y una orden al respecto, el Ministerio de Sanidad no había conseguido que los hospitales remitieran los casos sospechosos de tuberculosis a los dispensarios. A raíz de la epidemia de síndrome respiratorio agudo y grave de 2003, el gobierno creó un sistema nacional de notificación de enfermedades transmisibles a través de Internet, que consiguió que los hospitales y los dispensarios de atención de la tuberculosis desarrollaran una colaboración fructífera. Entre 2004 y 2007, el porcentaje de casos posibles o confirmados de tuberculosis derivados de los hospitales que accedieron a los dispensarios de tuberculosis aumentó considerablemente, del 58,7% al 77,8%, y la contribución de los hospitales para el diagnóstico de los casos de TB con baciloscopias positivas se duplicó del 16,3% al 32,9%. Gracias a la utilización del sistema de notificación a través de Internet, los hospitales de China han contribuido en la detección de aproximadamente un tercio de todos los casos de TB con baciloscopias positivas y han ayudado a cumplir el objetivo mundial de control de la tuberculosis, esto es, detectar el 70% de los casos. Basándose en los datos disponibles de vigilancia sistemática facilitados por este sistema basado en Internet, este artículo detalla el proceso y los resultados de la consolidación de la colaboración entre los hospitales y los dispensarios de tuberculosis que utilizan Internet como herramienta fundamental, así como su posible aplicación a otros países.

En Chine, les services de traitement de la tuberculose (TB) sont fournis par un large réseau de dispensaires de TB. Même si les hôpitaux ne sont pas bien placés pour suivre les normes recommandées en matière de soins de la TB, une part importante de personnes présentant les symptômes de la TB cherche à se faire soigner dans les hôpitaux. Bien que le ministère de la Santé dispose d'une politique et d'un mandat, il n'a que très peu réussi à encourager les hôpitaux à signaler les suspicions de TB aux dispensaires. Suite à l'épidémie du syndrome respiratoire aigu sévère (SRAS) en 2003, le gouvernement a mis en place un système de notification des maladies par Internet à l'échelle nationale. Ce système a permis d'obtenir une collaboration productive entre les hôpitaux et les dispensaires de TB. De 2004 à 2007, le pourcentage de suspicions et de cas avérés de TB nécessitant une orientation des patients des hôpitaux vers les dispensaires de TB a considérablement augmenté de 58,7 à 77,8%. De plus, la contribution des hôpitaux dans le diagnostic des cas de TB à frottis d'expectoration positifs a doublé, passant de 16,3 à 32,9%. En utilisant un système de notification par Internet, les hôpitaux chinois ont contribué à détecter environ un tiers de tous les cas de TB à frottis d'expectoration positifs et ont permis de répondre à l'objectif de contrôle de la TB global, qui est d'identifier 70% de ces cas. Reposant sur les données disponibles de la surveillance continue facilitée par ce système Internet, cet article décrit en détail le processus et les résultats d'une collaboration renforcée entre les hôpitaux et les dispensaires de TB qui utilisent Internet comme un outil ainsi que son application potentielle aux situations des autres pays.