Abstract Introduction Phagocytosis of autoantibody-sensitized coated platelets through Fc gamma receptors on phagocytic cells is an important mechanism of thrombocytopenia in primary immune thrombocytopenia (ITP). Objective We aimed to investigate the contribution of the FcγRIIa and FcγRIIIa genes polymorphism to the risk of ITP and their association with disease characteristics in Egyptian children. Methods A case control study was conducted on eighty children with primary ITP and eighty age and sex healthy matched subjects as a control group. The FcγRIIa and FcγRIIIa genes polymorphism was detected using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results We found that the FcγRIIa‐131H and ‐131R allele frequencies were 51.3 % and 48.7%, respectively, in children with ITP, versus 75% and 25%, respectively, in controls (p= 0.002). The compound heterozygous HR genotype was significantly higher in ITP patients (p < 0.05). The FcγRIIIa-158F and ‐158V allele frequencies were 46.3% and 53.7%, respectively, in children with ITP, versus 70% and 30%, respectively, in controls (p= 0.002). The compound heterozygous VF genotype was significantly higher in ITP patients (p < 0.05). The combined HR/FV genotype was 47.5% in ITP patients, versus 10% in controls (p < 0.001). No significant difference was found between children with newly diagnosed ITP and those who developed chronic ITP, regarding the frequency distribution of the FcγRIIa and FcγRIIIa alleles and genotypes (p > 0.05). Conclusion There is a possible association of the FcγRIIa and FcγRIIIa genes polymorphism with the risk for, and genetic susceptibility to ITP in Egyptian children, but large-scale studies are still needed to support our findings. autoantibodysensitized autoantibody sensitized ITP. . (ITP) group reactionrestriction reaction restriction PCRRFLP. PCRRFLP PCR RFLP (PCR-RFLP) FcγRIIa131H FcγRIIaH 131H H 131R R 513 51 3 51. 487 48 7 48.7% respectively 75 25 25% p= p 0.002. 0002 0.002 0 002 0.002) 0.05. 005 0.05 05 0.05) FcγRIIIa158F FcγRIIIaF 158F F 158V V 463 46 46.3 537 53 53.7% 70 30 30% HRFV FV 475 47 5 47.5 10 0.001. 0001 0.001 001 0.001) for largescale large scale findings (ITP (PCR-RFLP 4 48.7 2 000 0.00 00 0.0 46. 53.7 47. 1 48. 0. 53.

RESUMEN Se realizó un experimento de campo en lugares contrastantes de las estaciones de investigación agrícola Kafr-El-Hamam/Sharkia y Al-Arish/Norte del Sinaí en el Centro de Investigación Agrícola en Egipto. Se estimaron la variación genética aditiva y dominante, así como la heredabilidad en sentido amplio y restringido para el rendimiento y atributos de girasol en el cruce L350 x L355 usando North Carolina Design-III. La magnitud de las variaciones aditivas para todos los rasgos estudiados en ambos lugares en proporción a las variaciones fenotípicas fue mayor que las dominantes, y el grado promedio de dominancia fue mayor que la unidad, como también lo confirma la alta heredabilidad de sentido estrecho para la mayoría de los rasgos estudiados. La mejora genética se puede lograr mediante la selección de genotipos con mayor diámetro de capítulo y mayor peso de semilla que también mostraron valores, moderados a altos, con rasgos de heredabilidad de sentido estrecho. Treinta y dos retro cruzamientos de girasol se agruparon en ocho clústeres/grupos distintos mediante análisis canónico.

Abstract A field experiment was conducted in the contrasting locations of Kafr-El-Hamam/Sharkia and Al-Arish/North Sinai agricultural research stations of the Agricultural Research Center in Egypt. We estimated the additive and dominance genetic variances as well as heritability in the broad and narrow senses for sunflower yield and its attributes in the cross L350 x L355 using North Carolina Design-III. The magnitude of the additive variances for all studied traits at both locations in proportion to the phenotypic variances was larger than the dominance variances. The average degree of dominance was greater than the unit, as also confirmed by the high narrow-sense heritability for most of the studied traits. The genetic improvement may be achieved through the selection of genotypes with larger head diameter and heavier seed weight which also showed moderate to high values of narrow-sense heritability. Thirty-two sunflower backcrosses were grouped into eight distinct clusters/groups by canonical analysis.