ABSTRACT OBJECTIVE To describe the methodological challenges and strategies of a web survey on the working conditions and health among delivery workers. METHODS The study population consisted of Brazilian delivery workers operating in the national territory. Procedures include building solid and ongoing collaboration with worker representatives and conducting a four-month data collection from February to May 2022, sharing the link to the online questionnaire on social media such as social networks (Facebook, Instagram) and messaging apps (WhatsApp, Telegram). RESULTS The recruitment of 41 leaders or influencers of delivery workers increased the dissemination of the study, some of whom participated in the consensual validation of the questionnaire; the production of content for social media for the dissemination of the questionnaire link on social networks and applications, and the in-person dissemination of the study at the delivery workers’ meeting points during the workday played a fundamental role, totaling around 132 hours in 45 shifts. The strategies adopted for data collection with a hybrid approach to dissemination made it possible to carry out the web survey. After four months of the web survey, 564 delivery workers, 543 men and 18 women, responded to the online questionnaire. CONCLUSION The web survey presented methodological strategies to overcome the challenge of reaching workers, including hybrid work, to increase the participation of workers, on whom epidemiological research is still scarce.

ABSTRACT OBJECTIVE To describe the methodological challenges and strategies of a web survey on the working conditions and health among delivery workers. METHODS The study population consisted of Brazilian delivery workers operating in the national territory. Procedures include building solid and ongoing collaboration with worker representatives and conducting a four-month data collection from February to May 2022, sharing the link to the online questionnaire on social media such as social networks (Facebook, Instagram) and messaging apps (WhatsApp, Telegram). RESULTS The recruitment of 41 leaders or influencers of delivery workers increased the dissemination of the study, some of whom participated in the consensual validation of the questionnaire; the production of content for social media for the dissemination of the questionnaire link on social networks and applications, and the in-person dissemination of the study at the delivery workers’ meeting points during the workday played a fundamental role, totaling around 132 hours in 45 shifts. The strategies adopted for data collection with a hybrid approach to dissemination made it possible to carry out the web survey. After four months of the web survey, 564 delivery workers, 543 men and 18 women, responded to the online questionnaire. CONCLUSION The web survey presented methodological strategies to overcome the challenge of reaching workers, including hybrid work, to increase the participation of workers, on whom epidemiological research is still scarce. territory fourmonth month 2022 Facebook, Facebook (Facebook Instagram WhatsApp, WhatsApp (WhatsApp Telegram. Telegram . Telegram) 4 applications inperson person role 13 shifts 56 54 1 women work scarce 202 5 20 2

This work aims to investigate the influence of optimized welding parameters on the corrosion performance of the lean duplex stainless steel (LDSS) UNS S32101. The correlation between microstructure and electrochemical behavior of the fusion line (FL) and fusion zone (FZ) has been investigated. In the study, six welded samples were manufactured with different welding parameters, specifically the welding current, travel speed and heat input. A mini-cell and Syringe Cell were used to characterize the electrochemical behavior of the different welded zones by means of the double loop electrochemical potentiokinetic reactivation test (DL-EPR) and potentiodynamic polarization tests. The results showed that the welding parameters tested in this study significantly affected the corrosion resistance of the LDSS UNS S32101. The heat-affected zone (HAZ) was the most susceptible zone to localized corrosion and the specific region most affected by corrosion process was that of the LDSS UNS S32101 adjacent to the FL. (LDSS S FL (FL FZ (FZ investigated current input minicell mini cell DLEPR DL EPR (DL-EPR tests heataffected HAZ (HAZ S3210 S321 S32 S3

This work aims to compare the construction of magnification devices. Details of the construction and use of microscopes based on webcam and smartphone are presented, using software for image processing. It is also presented the construction of microscopes using drops of different liquids as magnifying lens, a compound microscope, and a water-drop projector. Magnified images obtained with each device are presented, and the different microscopes are compared. The results obtained from the use of these alternative instruments confirm that alternative microscopes are instruments of easy reproducibility, enabling the amplification of samples for a more detailed observation of the object of interest. devices processing lens microscope waterdrop water drop projector compared reproducibility interest

RESUMO A eficiência em tração do trator agrícola pode ser maximizada ajustando a massa total e sua distribuição entre os eixos. O objetivo do experimento foi determinar a configuração de distribuição de massa entre eixos e velocidade de deslocamento que proporcione maior eficiência em tração na operação de gradagem. O experimento foi conduzido no delineamento em blocos casualizados no esquema fatorial 2 × 3, com cinco repetições, sendo o primeiro fator duas distribuições de massa entre eixos e o segundo fator três marchas. Os dados coletados foram submetidos à análise de variância e ao teste de Tukey. A condição que maximiza o desempenho do trator corresponde a 39% da massa total no eixo dianteiro e 61% no eixo traseiro, com marcha que proporcione velocidade próxima a 10 km h-1.

ABSTRACT The traction efficiency of the agricultural tractor can be maximized by adjusting the total mass and its distribution between the axles. The experiment’s objective was to determine the configuration of mass distribution between axles and the displacement speed that provides greater traction efficiency in the harrowing operation. A randomized block design in a 2 × 3 factorial scheme with five replications was used. The first factor was two mass distributions between axles, and the second factor was three gears. The collected data were submitted to analysis of variance and the Tukey test. The condition that maximizes the tractor’s performance corresponds to 39% of the total mass on the front axle and 61% on the rear axle, with a gear that provides speed close to 10 km h-1.

Abstract Objective To assess the effect of intimate partner violence on the risk of depression and depressive symptoms among adult women. Method We analyzed data from the Mexican Health Workers' Cohort study (n=470). Type and severity of intimate partner violence was ascertained between 2004 and 2011. Self-reported medical diagnosis of depression (2011) was the main outcome; depressive symptoms ascertained with the Centre for Epidemiologic Studies-Depression (CES-D) scale was the secondary outcome. Random-effects regressions were run to model the risk of depression (logistic) and depressive symptoms (linear) in relation to intimate partner violence. Results 41.9% women experienced intimate partner violence at baseline. The incidence of depression was 7.2%. The risk of depression increased with any type of IPV (adjusted odds ratio [aOR]=2.9; 95% confidence interval [95%CI]: 1.4-6.2) and with physical (aOR=4.3; 95%CI: 1.8-10.1), psychological (aOR=3.1; 95%CI: 1.4-6.6) and sexual (aOR=3.1; 95%CI: 1.2-8.2) violence. Depressive symptoms (CES-D) increased slightly with physical and sexual intimate partner violence. Conclusions Intimate partner violence was associated with a higher risk of depression in this sample of women working in a Mexican health facility. Our results indicate the need to develop infrastructure, to implement strategies of attention and counselling, and to provide a safe environment in the workplace for women who experience intimate partner violence.

Resumen Objetivo Evaluar el efecto de la violencia de pareja, experimentada en los últimos 12 meses, sobre el riesgo de depresión y la sintomatología depresiva en mujeres adultas. Método Se analizaron los datos de 470 mujeres de la Cohorte de Trabajadores de la Salud de México. El tipo y la gravedad de la violencia de pareja se midieron en los años 2004 y 2011. El evento principal fue el diagnóstico médico de depresión (2011). Un evento secundario fue la sintomatología depresiva (2004 y 2011), evaluada mediante la Escala de Depresión del Centro de Estudios Epidemiológicos (CES-D). El riesgo de depresión y la sintomatología depresiva en relación con la violencia se evaluaron con modelos de regresión de efectos aleatorios logísticos y lineales, respectivamente. Resultados El 41,9% experimentó violencia de pareja en la medición basal. La incidencia de depresión fue del 7,2%. El riesgo de depresión aumentó con la violencia de pareja de cualquier tipo (odds ratio ajustada [ORa]=2,9; intervalo de confianza del 95% [IC95%]: 1,4-6,2) y con las violencias física (ORa=4,3; IC95%: 1,8-10,1), psicológica (ORa=3,1; IC95%: 1,4-6,6) y sexual (ORa=3,1; IC95%: 1,2--8,2). Los síntomas depresivos (CES-D) aumentaron ligeramente con las violencias física y sexual. Conclusiones La violencia de pareja se asoció a un mayor riesgo de depresión en mujeres trabajadoras de una institución de salud de México. Los resultados indican la necesidad de contar con infraestructura, implementar estrategias de atención y consejería, y brindar un ambiente seguro en los lugares de trabajo para quienes sufren violencia de pareja.

Abstract Objective To assess the effect of intimate partner violence on the risk of depression and depressive symptoms among adult women. Method We analyzed data from the Mexican Health Workers' Cohort study (n=470). Type and severity of intimate partner violence was ascertained between 2004 and 2011. Self-reported medical diagnosis of depression (2011) was the main outcome; depressive symptoms ascertained with the Centre for Epidemiologic Studies-Depression (CES-D) scale was the secondary outcome. Random-effects regressions were run to model the risk of depression (logistic) and depressive symptoms (linear) in relation to intimate partner violence. Results 41.9% women experienced intimate partner violence at baseline. The incidence of depression was 7.2%. The risk of depression increased with any type of IPV (adjusted odds ratio [aOR]=2.9; 95% confidence interval [95%CI]: 1.4-6.2) and with physical (aOR=4.3; 95%CI: 1.8-10.1), psychological (aOR=3.1; 95%CI: 1.4-6.6) and sexual (aOR=3.1; 95%CI: 1.2-8.2) violence. Depressive symptoms (CES-D) increased slightly with physical and sexual intimate partner violence. Conclusions Intimate partner violence was associated with a higher risk of depression in this sample of women working in a Mexican health facility. Our results indicate the need to develop infrastructure, to implement strategies of attention and counselling, and to provide a safe environment in the workplace for women who experience intimate partner violence.

Resumen Objetivo Evaluar el efecto de la violencia de pareja, experimentada en los últimos 12 meses, sobre el riesgo de depresión y la sintomatología depresiva en mujeres adultas. Método Se analizaron los datos de 470 mujeres de la Cohorte de Trabajadores de la Salud de México. El tipo y la gravedad de la violencia de pareja se midieron en los años 2004 y 2011. El evento principal fue el diagnóstico médico de depresión (2011). Un evento secundario fue la sintomatología depresiva (2004 y 2011), evaluada mediante la Escala de Depresión del Centro de Estudios Epidemiológicos (CES-D). El riesgo de depresión y la sintomatología depresiva en relación con la violencia se evaluaron con modelos de regresión de efectos aleatorios logísticos y lineales, respectivamente. Resultados El 41,9% experimentó violencia de pareja en la medición basal. La incidencia de depresión fue del 7,2%. El riesgo de depresión aumentó con la violencia de pareja de cualquier tipo (odds ratio ajustada [ORa]=2,9; intervalo de confianza del 95% [IC95%]: 1,4-6,2) y con las violencias física (ORa=4,3; IC95%: 1,8-10,1), psicológica (ORa=3,1; IC95%: 1,4-6,6) y sexual (ORa=3,1; IC95%: 1,2--8,2). Los síntomas depresivos (CES-D) aumentaron ligeramente con las violencias física y sexual. Conclusiones La violencia de pareja se asoció a un mayor riesgo de depresión en mujeres trabajadoras de una institución de salud de México. Los resultados indican la necesidad de contar con infraestructura, implementar estrategias de atención y consejería, y brindar un ambiente seguro en los lugares de trabajo para quienes sufren violencia de pareja.

Abstract Paper aims Lack of operational planning in healthcare organizations may result in service disconnection and reduced value per effort to the patient. Production Planning, Scheduling and Control assists in determining “what,” “how much,” “when” and “where” to deliver, which may be of great relevance to healthcare services. This study proposes a structural framework and analyzes the implementation of PPSC concepts in OR management. Originality The application of concepts from manufacturing applied to healthcare as the PPSC, present in this text, demonstrates the crossing and alignment of global management techniques and tools applicable to both areas. Research method The discussion is based on data gathered through action-research in a Brazilian public hospital. Main findings In the hospital under study, the PSCPF implementation resulted in increased production control of surgeries, increased efficiency in management of waiting lists, increased operational efficiency, and motivational gains from staff integration. Implications for theory and practice The understanding and proper implementation of the concept of PPSC contributed to mitigate problems related to capacity management, reduced the unawareness of different levels of demand and waiting lists, cut down scheduling errors, and prevented the provision of false information from the disconnection between the various areas of the hospital.

Abstract The objective of this study was to evaluate whether the addition of a blend based on α-monolaurin mono-, di- and triglycerides of butyric acid, and lysolecithin on the performance even on diets containing reduced inclusion of oil in the diet and without the use of growth-promoting antibiotics of broilers considering the effect on health, performance, and meat. Three treatments were defined: positive control (TP: with enramycin), negative control (TN: no enramycin), and blend (T-FRA: with monolaurin and glycerides of acid butyric minus 0.8% soybean oil). At 21 days, broilers treated with TP and T-FRA obtained the lower feed conversion ratio (FC); at 35 days, T-FRA broilers obtained lower FC than TN broilers. Cholesterol levels were higher in the blood of T-FRA broilers. On day 42, levels of ROS and TBARS were lower in the intestine, muscles, and liver of T-FRA broilers. Moreover, glutathione S-transferase and total non-enzymatic antioxidants were greater at the intestinal and muscular levels. The T-FRA broilers had a lower percentage of lipids in the meat. The MIC indicated that 111mg of the blend/mL inhibited the growth of E. coli; however, the counts of total coliforms and E. coli in the feces and the broilers’ litter did not differ between treatments. In conclusion, the addition of the blend T-FRA in broiler diets was able to improve the feed conversion and maintain the other performance parameters even considering a reduction of 0.8% in the inclusion of oil.

RESUMO A resistência à penetração do solo (RSP) é empregada como indicadora da compactação, por estar relacionada a importantes atributos do solo e das plantas, e por ser uma medida de fácil obtenção. Sua determinação com o uso do penetrômetro orienta para as melhores estratégias de manejo do solo, favorecendo assim o desenvolvimento das culturas. O objetivo deste estudo foi desenvolver e validar o penetrômetro eletrônico de acionamento hidráulico georreferenciado, facilitando a obtenção da RSP. Para tanto foram realizadas 36 leituras de 0 a 0,60 m de profundidade da RSP com os penetrômetros manual e hidráulico em duas áreas. Os resultados de RSP foram analisados estabelecendo-se os intervalos de confiança pelo teste t (p ≤ 0,10), a cada 0,05 m de profundidade, e o georreferenciamento através da raiz do erro quadrático médio (RSME). Constatou-se que ambos penetrômetros apresentaram similaridade na mensuração da RSP e do georreferenciamento dos pontos.

ABSTRACT Soil penetration resistance (SPR) is used as an indicator of compaction, as it is related to important soil and plant attributes and is an easily obtainable measure. Its determination with the use of the penetrometer guides to the best soil management strategies, thus favoring crop development. The objective of this study was to develop and validate the georeferenced hydraulic driven electronic penetrometer, making it easier to obtain SPR. For this, 36 SPR readings from 0 to 0.60 m depth were performed with the manual and hydraulic penetrometers in two areas. The SPR results were analyzed by establishing the confidence intervals by the t-test (p ≤ 0.10) at each 0.05 m depth and the georeferencing was analyzed through the root mean square error (RMSE). It was found that both penetrometers showed similarity in SPR measurement and in the georeferencing of the points.

ABSTRACT The operational performance of the agricultural tractor can be increased by adjusting parameters such as tire inflation pressure, axle mass distribution and gauge opening, which is a very divergent factor in controlled-traffic production. The objective of the experiment was to measure the energy performance of a 93 kW agricultural tractor in intermediate grading operation in two slopes (flat and sloping) and three gauge configurations (closed, intermediate and open) in mobilized soil. Energy performance was determined from the following parameters: slip, engine speed, actual effective speed, hourly and specific fuel consumption; strength, power and performance on the drawbar. Turbo pressure and temperature monitoring was also performed at six different engine points. The experiment was conducted in double factorial arrangement (2 slopes and 3 gauges), with four replications, totaling 24 plots. Data were analyzed for normality and homoscedasticity of the residues, after ANOVA and when significant, the means test. Tilts and tire gauges did not differ statistically, so it can be concluded that depending on the operation do not interfere with operating performance.

Abstract Our aim was to develop and apply a comprehensive noninvasive prenatal test (NIPT) by using high-coverage targeted next-generation sequencing to estimate fetal fraction, determine fetal sex, and detect trisomy and monogenic disease without parental genotype information. We analyzed 45 pregnancies, 40 mock samples, and eight mother-child pairs to generate 35 simulated datasets. Fetal fraction (FF) was estimated based on analysis of the single nucleotide polymorphism (SNP) allele fraction distribution. A Z-score was calculated for trisomy of chromosome 21 (T21), and fetal sex detection. Monogenic disease detection was performed through variant analysis. Model validation was performed using the simulated datasets. The novel model to estimate FF was robust and accurate (r2= 0.994, p-value < 2.2e-16). For samples with FF > 0.04, T21 detection had 100% sensitivity (95% CI: 63.06 to 100%) and 98.53% specificity (95% CI: 92.08 to 99.96%). Fetal sex was determined with 100% accuracy. We later performed a proof of concept for monogenic disease diagnosis of 5/7 skeletal dysplasia cases. In conclusion, it is feasible to perform a comprehensive NIPT by using only data from high coverage targeted sequencing, which, in addition to detecting trisomies, also make it possible to identify pathogenic variants of the candidate genes for monogenic diseases.

The amorphous, mesoporous SiO2-SO3H catalyst with a surface area of 115 m2 g-1 and 1.32 mmol H+ per g was very efficient for the protonation of ketones on a 10% (m/m) basis, and the catalyst-bound intermediates can be trapped by polyalcohols to produce ketals in high yields or suffer aldol condensations within minutes under low-power microwave irradiation. The same catalyst can easily reverse the ketalization reaction.

A new online solid phase preconcentration method using the new SiO2/Al2O3/SnO2 ternary oxide (designated as SiAlSn) as chelating agent free-solid phase extractor (CAF-SPE) coupled to flame atomic absorption spectrometry (FAAS) for Pb2+ determination at trace levels in different kind of samples is proposed. The solid adsorbent has been characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron miscroscopy (SEM), energy dispersive X-ray spectroscopy (EDS), X-ray fluorescence spectroscopy (XRF) and textural data. The method involves the preconcentration using time-based sampling of Pb2+ solution at pH 4.3 through 100.0 mg of packed adsorbed into a mini-column under flow rate of 4.0 mL min-1 during 5 min. The elution step was accomplished by using 1.0 mol L-1 HCl. A wide range of analytical curve (5.0-400.0 µg L-1), high enrichment factor (40.5), low consumption index (0.5 mL) and low limits of quantification and detection, 5.0 and 1.5 µg L-1, respectively, were obtained with the developed method. Practical application of method was tested on water samples, chocolate powder, Ginkgo biloba and sediment (certified reference material). On the basis of the results, the SiAlSn can be considered an effective adsorbent belonging to the class of CAF-SPE for Pb2+ determination from different matrices.

Abstract CHIME syndrome is an extremely rare autosomal recessive multisystemic disorder caused by mutations in PIGL. PIGL is an endoplasmic reticulum localized enzyme that catalyzes the second step of glycosylphosphatidylinositol (GPI) biosynthesis, which plays a role in the anchorage of cell-surface proteins including receptors, enzymes, and adhesion molecules. Germline mutations in other members of GPI and Post GPI Attachment to Proteins (PGAP) family genes have been described and constitute a group of diseases within the congenital disorders of glycosylation. Patients in this group often present alkaline phosphatase serum levels abnormalities and neurological symptoms. We report a CHIME syndrome patient who harbors a missense mutation c.500T > C (p.Leu167Pro) and a large deletion involving the 5’ untranslated region and part of exon 1 of PIGL. In CHIME syndrome, a recurrent missense mutation c.500T > C (p.Leu167Pro) is found in the majority of patients, associated with a null mutation in the other allele, including an overrepresentation of large deletions. The latter are not detected by the standard analysis in sequencing techniques, including next-generation sequencing. Thus, in individuals with a clinical diagnosis of CHIME syndrome in which only one mutation is found, an active search for a large deletion should be sought.