ABSTRACT. Waste oil from olive oil extraction industry was used, instead of soybean oil, in heavy roosters’ diet in order to evaluate birds’ reproductive parameters. A total of forty roosters were housed individually in boxes with 1.2 m². Two experimental diets were used: control diet, based on corn, soybean meal, and soybean oil; and test diet, where soybean oil was totally replaced by waste oil. In order to verify weight gain and feed intake, animals were individually weighed weekly. Seven semen collections were performed with fifteen-day interval. Reproductive variables analyzed sperm volume, motility, concentration, and morphology. No statistical difference (p > 0.05) was observed between treatments at the different collection periods for the variables sperm volume, motility, and concentration. There was a statistically significant difference between treatments for body weight in periods three (p = 0.04), and seven (p = 0.04). Statistical differences (p = 0.01) were also observed between treatments for abnormal sperm morphology. Among collection periods, statistical difference was observed for motility (p = 0.00), and sperm concentration (p = 0.01). Total replacement of soybean oil by waste oil from olive oil extraction in young heavy roosters’ diets does not affect sperm volume, motility, and concentration; reduces defects in sperm tail, and promotes better weight gain control.

Foram avaliados os efeitos da aplicação de Impact P® sobre a cama de aviário de matrizes de corte mediante o monitoramento de parâmetros microbiológicos. O Impact P® é um produto formulado através de Bacillus subtillis e suas enzimas que atuam sobre os dejetos animais e matéria orgânica, utilizando-os como fonte alimentar, visando melhorar as condições da cama dentro de aviários. Na 58ª semana de idade das matrizes foi trocado todo material de cama e então aplicados os seguintes tratamentos: T1- controle; T2 - Impact P® na dosagem de 2,5gm-2 de cama; T3 - Impact P® na dosagem de 5,0g m-2 de cama. Foram realizadas contagens de enterobactérias totais em agar McConkey em amostra de cama nova e após 3, 10, 17, 24 e 31 dias de uso da cama pelas aves e tratamentos com Impact P®. A análise estatística foi realizada no procedimento General Linear Models - GLM que utiliza o método dos quadrados mínimos, em um esquema fatorial 3x4 (tratamento x tempo de uso da cama) em delineamento completamente casualizado, com quatro repetições por tratamento, sendo as médias comparadas pelo teste de Tukey. A dosagem de 5,0g de Impact P® por m² de cama foi eficiente para reduzir em aproximadamente 13% a contagem logarítmica total de enterobactérias em comparação com o grupo não tratado (2,89 versus 3,31log10 (UFC), respectivamente, P<0,05).

This study aimed to evaluate the utilization of Impact P® in used litter from floor pens of broiler breeders through the monitoring of microbiological parameters. Impact P® is a commercial product obtained from Bacillus subtillis and it enzymes. Using animal litter and organic matter as a feed source, the product was designed to improve the quality of floor pens of broiler breeders. When hens aged 58 weeks, all used floor pens material was replaced with a new one and the following treatments were tested: T1 - control; T2 - 2.5g Impact P® m-2 litter; T3- 5.0g Impact P® m-2 litter. Counting of number of enterobacterium colonies in agar Mac-ConcKey was performed in a sample of new floor pens material and after 3, 10, 17, 24 and 31 days of litter treatment with Impact P®. The statistical analysis was accomplished with the General Linear Models - GLM procedure, using the minimum square method in a 3x4 factorial (treatment vs. time of floor pens utilization), in a completely randomized design with four replications. The averages were compared by Tukey test. The utilization of 5.0g Impact P® m-2 litter was efficient to reducing approximately 13% logarithmic total counting of the enterobacterium in comparison to the untreated group (2.89 versus 3.31log10 (CFU), respectively, P<0.05).