Resumo Para explorar os atributos físicos e químicos do solo e espacializar o estoque de carbono orgânico do solo em um trecho de Floresta Atlântica (Estacional Semidecidual Submontanal, Espírito Santo, Brasil), este estudo avaliou quatro camadas de solo (0-5, 5-10, 10-20 e 20-40 cm), as inter-relações dos atributos físicos e químicos e a espacialização do estoque de carbono orgânico do solo aplicando a interpolação por “cokrigagem”. Foram coletadas 12 amostras em cada camada de solo, sendo determinados os teores de nutrientes, a densidade do solo, a textura e o teor de carbono orgânico, sendo também determinado o estoque de carbono. Os atributos do solo mais significativos na variação dos dados foram verificados pela ordenação por Análise de Componentes Principais (ACP) agrupando as parcelas semelhantes quanto às características físicas e químicas. Com base na autocorrelação espacial dos atributos do solo, os dados do modelo de elevação digital (altitude e declividade) para o local estudado foram combinados para explorar a relação de coordenadas entre os parâmetros do terreno e o estoque de carbono orgânico do solo. Foi verificado o aumento da fertilidade do solo paralelo ao aumento do teor de matéria orgânica, auxiliando no entendimento das diferenças e similaridades dos sítios identificados no campo. Observa-se diferença espacial no estoque de carbono orgânico do solo, sendo o maior verificado nas áreas de menor declividade e altitude.

Abstract This study aimed to explore the physical and chemical attributes of the soil and to spatialize the soil organic carbon stock in a stretch of Atlantic Forest by evaluating four soil layers and the interrelationships of physical and chemical attributes as well as the spatialization of soil organic carbon stock by applying cokriging interpolation. Thus, 12 soil samples were collected in a soil layer, and the nutrient content, soil density, texture, soil organic carbon content and the soil carbon stock were determined. A Principal Component Analysis (PCA) grouped the most similar plots in terms of physical and chemical characteristics. Based on the spatial autocorrelation of the soil attributes, the digital elevation model data (altitude and slope) for the studied site were combined to explore the coordinate relationship between the terrain parameters and the soil organic carbon stock. We verified an increase in the soil fertility parallel to the increase in the organic matter content in the soil, helping to understand the differences and similarities of the identified sites in the field. There is a spatial difference in the soil organic carbon stock, with the largest being observed in areas of lower slope and altitude. The physical and chemical attributes of the soil in the Atlantic Forest varied according to the sampled points, being strongly related to the relief. The spatial variability altered soil organic carbon stocks in the forest under study.

Abstract The objective of this study was to analyse the initial medium-term growth of a P. reticulata settlement under different phosphorus doses. The experiment was carried out in an area of Atlantic Forest, Sooretama, Northern Espírito Santo State, Brazil. Five doses of P per each tree (0 g, 27 g, 47 g, 67 g and 87 g) were tested in a randomized block design with three replicates and 18 plants per plot. At 3 months, 9 months and 15 months, height and diameter were measured at the soil level. At 36 months height, diameter at breast height, survival, basal area, and nutritional evaluation of the trees were measured. A logistic model was also used to model the growth in height of individual trees and a logistic model considering fixed effects and mixed form (fixed and random effects), in order to verify if the inclusion of the random effect (tree) would result in gains in terms of accuracy in the estimation of the future height of P. reticulata trees. The species presents potential for use in reforestation programs under edaphoclimatic conditions tested and under similar conditions. The species was not very demanding in P, and the addition of this nutrient promotes negative effects on its growth in the short and medium term. Regarding the model of growth in height, the logistic model adjusted in the mixed form showed to be superior to the traditional adjustment.

Resumen El objetivo de este estudio fue analizar el crecimiento inicial a mediano plazo de un asentamiento de P. reticulata bajo diferentes dosis de fósforo. El experimento se llevó a cabo en un área del Bosque Atlántico, Sooretama, norte del estado de Espírito Santo, Brasil. Se probaron cinco dosis de P por árbol (0 g, 27 g, 47 g, 67 g y 87 g) en un diseño de bloques al azar con tres repeticiones y 18 plantas por parcela. A los 3 meses, 9 meses y 15 meses se midió la altura y el diámetro a nivel del suelo. A los 36 meses de la siembra, se midió el diámetro a la altura del pecho, la supervivencia y el área basal y se hizo una evaluación nutricional de los árboles. También se utilizó un modelo logístico para modelar el crecimiento en altura de árboles individuales y el modelo logístico considerando efectos fijos y forma mixta (efectos fijos y aleatorios) para verificar si la inclusión del efecto aleatorio (árbol) resultaría en ganancias en términos de precisión en la estimación de la altura futura de los árboles de P. reticulata. La especie presenta potencial para su uso en programas de reforestación en condiciones edafoclimáticas probadas y en condiciones similares. La especie no fue muy exigente en fósforo y la adición de este nutriente promovió efectos negativos en su crecimiento a corto y mediano plazo. Con respecto al modelo de crecimiento en altura, el modelo logístico ajustado en forma mixta mostró ser superior al ajuste tradicional.

ABSTRACT: The evaluation of diversity and genetic structure allows us to verify with precision the effect of evolutionary and anthropic processes on species. The objective of this research was to evaluate the divergence and the genetic structure of two natural populations of Dalbergia nigra, using molecular markers Inter Simple Sequence Repeats (ISSR). Leaf samples were collected from two populations, located in the National Forest of Pacotuba and the Private Natural Heritage Reserve of Cafundó. Eight ISSR primers were used, which resulted in 97 bands, with 68.04% of polymorphism. Based on the joint data, the values of 0.33 for the Nei index (H*) and 0.50 for the Shannon index (I*) indicated moderate to high genetic diversity, being influenced by the presence of genetically dissimilar individuals in the National Forest of Pacotuba. Most of the genetic divergence was intrapopulational (85.96%), with moderate differentiation between populations (ΦST = 0.1404). The estimated historical gene flow between the fragments was low (Nm = 3.21) when compared with results from other tree species, and the genetic structuring analysis separated the populations into two groups, corresponding to the two populations evaluated. The results indicate a small genetic share among populations, however, populations are becoming structured. The satisfactory levels of genetic diversity benefit the use of the trees as matrixes for programs of restoration and recovery of degraded areas, connectivity of landscapes, and sustainable use of forest resources.

Resumo As características físico-químicas dos frutos de espécies nativas da Floresta Atlântica são pouco conhecidas, especialmente durante seu amadurecimento. O objetivo deste trabalho foi avaliar o efeito da interação entre o período de maturação e o armazenamento pós-colheita de araçaúna e grumixama. Os frutos foram colhidos em três estádios de maturação: verdes, parcialmente maduros e maduros, e separados em dois lotes, armazenados por 2 e 48 horas. Foram avaliados: diâmetro longitudinal (LD) e transversal (CD) dos frutos, relação LD/CD, massa fresca dos frutos (FM), rendimento em polpa (PY), pH, sólidos solúveis (SS), acidez titulável (TA), ratio (SS/TA) e ácido ascórbico (AA). Os frutos de araçauneira destacaram-se por ter, em média, elevado PY (74,43%), com baixo SS (2,07 ºBrix) e alta TA (1,17% de ácido cítrico). Porém, a colheita dos frutos totalmente maduros aumenta o SS em 21% e reduz a TA em 32,5%, em comparação à média. Os frutos de grumixameira são levemente adocicados (6,06 ºBrix), com baixa acidez (0,39% de ácido cítrico), ricos em vitamina C (206,94 mg de ácido ascórbico/100 g de polpa, e sua colheita deve ser feita quando os frutos estiverem totalmente maduros. Ambas espécies possuem polpas de qualidade potencial para serem exploradas comercialmente em produtos processados

Abstract The physicochemical characteristics of fruits of species native to the Atlantic Forest are little known, especially during ripening. The aim of this work was to evaluate the effect of the interaction between ripening period and post-harvest storage of araçaúna and grumixama. Fruits were harvested at three ripening stages: green, partially ripe and ripe, according to the epicarp color, and separated into two groups, being stored for 2 and 48 hours. The following variables were evaluated: longitudinal (LD) and cross-sectional (CD) diameter, LD / CD ratio, fresh fruit mass (FM), pulp yield (PY), pH, soluble solids content (SS), titratable acidity (TA), SS / TA ratio, and ascorbic acid content (AA). Araçaúna fruits stand out due to their high PY (74.43%), with low SS (2.07 ºBrix) and high TA (1.17% citric acid). However, harvesting fully ripe fruits increases SS by 21% and reduces TA by 32.5%. Grumixama fruits are slightly sweet (6.06 ºBrix), with low acidity (0.39% citric acid), high vitamin C content (206.94 mg of ascorbic acid/100g of pulp), and harvest must be performed when fruits are fully ripe. Both species have pulp of potential quality to be commercially exploited, especially for processing.

ABSTRACT The objective of this work was to understand the relationship between decomposition of leaf litter and climatic elements and determine the mineralization of nutrients in the decomposition process in a fragment of Semideciduous Submontane Forest, located in southern state of of Espírito Santo. Overall, 84 litterbags were deposited in 12 permanent plots, collecting one litterbag per plot for 30, 60, 90, 150, 210, 270 and 360 days after the beginning of the experiment. The leaf material remaining in the litterbag was oven-dried and weighed, with later analysis of nutritional compounds. The leaf litter decomposition rate was 1.42, 75.95% of decomposing litter at the end of the evaluation period. Mineralization occurred in the following order: K, Corg., Mg, S, P, N and Ca. There was a positive correlation of average and minimum air temperature, as well as accumulated precipitation and leaf litter decomposition .

ABSTRACT The objective of the present study was to evaluate the influence of spacing, species richness, and sampling time on accumulated litter in forest restoration areas in the state of Espírito Santo, Brazil. The experiment was conducted under a randomized complete block design, in a subdivided plot scheme. Six treatments were evaluated, consisting of a combination of spacing levels and species richness. Accumulated litter samples were weighed and chemically analyzed to obtain nutrient concentrations. The variables analyzed in the restoration process, in general, did not influence litter biomass and nutrient concentrations. The time elapsed between restoration implantation and data sampling for the present study may have been insufficient for the occurrence of differences in variables evaluated. Despite the small amount of accumulated litter, it is of great importance for soil physical protection and fertility improvement, generating conditions for the stabilization of new forest species in the restoration area.

Resumo A seleção de genótipos superiores com base na resposta simultânea à diferentes caracteres é uma estratégia fundamental no melhoramento de plantas. Neste estudo objetivou-se comparar a eficiência de quatro índices de seleção construídos a partir de valores fenotípicos e genotípicos, em uma população segregante da cultivar Rubi Incaper 511. Foram avaliadas oito variáveis morfoagronômicas e a severidade de pinta-preta e mancha-de-phoma em condições de campo. Os índices clássicos de seleção foram calculados com base na média fenotípica não padronizada (NSM), padronizada (SM) e nos valores genotípicos preditos via REML/Blup (GVP), utilizando pesos econômicos pré-determinados. Adicionalmente, foi obtida a soma de ranks (RS), considerando o ordenamento dos indivíduos nesses três indices de seleção. Em dez características, os indivíduos selecionados melhoraram a média em relação à população original. Os melhores valores de diferencial de seleção foram obtidos pelo SM, porém, o maior grau de coincidência entre os indivíduos selecionados foi obtido entre GVP e RS (80%). Os índices utilizados foram eficientes para selecionar os indivíduos com maior teor de sólidos solúveis e menor severidade das doenças avaliadas. Os diferentes índices de seleção devem ser avaliados quanto a sua eficiência na obtenção de ganhos genéticos, buscando aquele que trará melhores resultados aos programas de melhoramento do mamoeiro.

Abstract Selection of superior genotypes based on the simultaneous responses to different characteristics is a fundamental strategy in plant breeding. This study aimed to compare the efficiency of four selection index constructed using phenotypic and genotypic values in a segregating population of the cultivar Rubi Incaper 511. Eight morpho-agronomic variates and the severity of black-spot and phoma-spot were evaluated under field conditions. The classical selection index were calculated based on non-standardized phenotypic means (NSM), standardized means (SM), and genotypic values predicted by REML/BLUP (GVP), using predetermined economic weights. Additionally, the rank sum (RS) was obtained on the basis of the classification of individuals in these three selection index. For ten characteristics, the selected individuals showed a higher mean than did the original population. The best selection differential values were obtained by SM, however, the highest degree of coincidence among the selected individuals was obtained between GVP and RS (80%). The index used were efficient at selecting individuals with higher soluble solids and lower diseases severity evaluated. Therefore, for the papaya breeding programs, different selection index need to be evaluated to maximize genetic gain.

RESUMOObjetivou-se com o trabalho avaliar a influência do reflorestamento com essências nativas da Floresta Estacional Semidecidual, plantio de eucalipto e pastagem nos atributos químicos e físicos do solo de áreas degradadas. Foram selecionadas cinco áreas na bacia hidrográfica do Rio Aguapeí, na cidade de Garça, SP, sendo dois reflorestamentos com essências nativas, com 4 e 9 anos, um plantio de eucalipto, uma área de pastagem e um fragmento florestal utilizado como referência. O fragmento florestal apresentou a menor soma de bases e os maiores teores de P, Corg e CTC. Ele também apresentou a menor resistência à penetração, maior diâmetro e estabilidade dos agregados. Os atributos químicos e físicos do solo das áreas reflorestadas diferiram dos observados no fragmento florestal, mostrando que o período de recuperação não foi suficiente para restaurar esses atributos. Porém o reflorestamento com 9 anos melhorou significativamente nesses atributos do solo, tendendo a aproximar-se aos do fragmento florestal.

ABSTRACTThe objective with this study was to evaluate the influence of reforestation with native species from seasonal semideciduous forest, eucalyptus plantation, and pasture in the soil chemical and physical properties of degraded areas. Five sites were selected in the watershed of the Aguapeí River in the Garça city, Sao Paulo state: two reforestation areas with native species, with four and nine years old; a reforestation area with eucalyptus plantation, a pasture area, and a forest fragment used as control. The forest fragment presented the lowest exangeable bases and the highest levels of P, SOC and CEC; it also showed the lowest penetration resistance, and the largest diameter and aggregate stability. The chemical and physical soil attributes of the reforestation areas differed from those of the forest fragment (control), showing that the recovery period was not sufficient to restore these attributes. However, the 9-year-old reforestation area presented significant improvement of these soil attributes, tending to approach those of the control area.

Por fazer parte de inúmeros compostos e estar diretamente relacionado com a capacidade fotossintética das plantas, o nitrogênio (N) pode influenciar significativamente na produção e enraizamento de estacas. Com base nesse contexto, o trabalho teve por objetivo avaliar o efeito de doses crescentes de N na produtividade, capacidade e velocidade de enraizamento das miniestacas produzidas de minicepas do clone híbrido de Eucalyptus grandis x Eucalyptus urophylla (I-224), além da qualidade das mudas formadas. O presente trabalho foi realizado no viveiro de mudas clonais de Eucalyptus, em minijardim de leito de areia da FAEF - Garça - SP. O delineamento experimental utilizado foi o inteiramente casualizado com 4 repetições, sendo os tratamentos constituídos por 5 doses de nitrogênio diferentes na solução nutritiva (0; 80; 160; 240 e 320 mgL-1). As variáveis analisadas foram: número de estacas produzidas, índice de produtividade, enraizamento das estacas produzidas e determinação da massa seca das mudas. A dose de nitrogênio aplicada no minijardim influenciou a produção de estacas, apresentando comportamento quadrático, atingindo a produção máxima (13,1 estacas cepa-1 mês-1) com a dose estimada de 129 mg L-1. O percentual de enraizamento apresentou comportamento linear crescente. A dose que proporcionou o maior índice de produtividade do minijardim foi de 194 mg L-1. A dose de nitrogênio aplicada no minijardim influenciou significativamente na velocidade de enraizamento, e o acúmulo de matéria seca das mudas formadas.

For being part of innumerable compounds and being directly related with the photosynthetic capacity of plants, Nitrogen (N) can significantly influence the production and rooting of cuttings. On the basis of this context, the work aimed to evaluate the effect of increasing doses of N in the productivity, capacity and speed of rooting mini-cuttings produced from mini-stumps of hybrid clones of Eucalyptus grandis x E. urophylla (I-224), beyond the quality of the formed changes. The present work was carried through in nursery seedlings of Eucalyptus, in a mini-garden of sand stream bed of FAEF, in Garça, São Paulo state. The used experimental delineation was complete randomized design with 4 replications, being the treatments consisted of 5 different doses of Nitrogen in nutritional solution (0; 80; 160; 240 and 320 mg L-1). The variables analyzed were: number of cuttings produced, the productivity index, rooting of cuttings produced and determination of the dry mass of seedlings. The dose of nitrogen applied in the mini-garden influenced the production of stakes, with a quadratic, reaching maximum production (13,1 stakes strain-1 month-1) with the estimated dose of 129 mg L-1.The percentage of rooting presented increasing linear behavior. The dose that provided the biggest index of productivity of the mini-garden was of 194 mg L-1. The dose of nitrogen applied in the mini-garden influenced the rooting significantly and the dry mass of formed seedlings.

Objetivou-se, com o trabalho, quantificar o acúmulo de serapilheira, bem como os macronutrientes e o carbono orgânico na Floresta Estacional Semidecidual Submontana, localizada em Cachoeiro de Itapemirim, ES. A amostragem da serapilheira foi realizada em 12 parcelas (20 m x 50 m), sendo as mesmas distribuídas de forma sistemática no campo. Em cada uma das 12 parcelas, foram coletadas mensalmente 12 amostras de serapilheira acumulada, de forma aleatória, com o auxílio de um gabarito de metal de 0,25 m x 0,25 m. O estudo de acúmulo de serapilheira na floresta foi analisado segundo o delineamento inteiramente casualizado, com 12 tratamentos (meses do ano) e 12 repetições. O acúmulo de serapilheira mostrou-se sazonal, com maiores acúmulos, ocorrendo no final da estação seca e de menores temperaturas do ar (Dez/09, Jan/10, Set/10 e Out/10), contendo em média 5,5 Mg ha-1 de serapilheira acumulada. O Cálcio foi encontrado em maior teor e conteúdo, sendo o conteúdo médio de macronutrientes contidos na serapilheira de 293,59 kg ha-1.

The objective of the study was to quantify the accumulation of litter, macronutrients and organic carbon in the Submontane Seasonal Semideciduous Forest, located in Cachoeiro de Itapemirim, ES. A sampling of litter was performed in 12 plots (20 m x 50 m), and was distributed systematically in the field. In each of the 12 plots were collected monthly 12 samples of litter accumulated at random, with the aid of a metal template of 0.25 m x 0.25 m. The study of accumulation of litter in the forest was analyzed according to completely randomized design with 12 treatments (months of the year) and 12 repetitions. The accumulation of litter was found to be seasonal, with higher accumulations occurring at the end of the dry season and lower air temperatures (Dec/09, Jan/10, Sep/10 and Oct/10), containing on average 5.5 Mg ha-1 of accumulated litter. Calcium was found in higher level and content, and the average content of macronutrients in the litter was 293.59 kg ha-1.

Objetivou-se estimar a biomassa e carbono orgânico em plantios de Pinus taeda L. com diferentes idades (14, 16, 19, 21, 22, 23 e 32 anos), localizados no município de General Carneiro, PR. Para estimar a biomassa e o carbono orgânico nos diferentes componentes das árvores (acículas, galhos vivos, galhos mortos, casca e madeira do fuste) foi utilizado o método destrutivo de quantificação, utilizando-se para tanto a amostragem de sete árvores distribuídas aleatoriamente pelo plantio em cada uma das idades. O estoque de biomassa e carbono orgânico nas diferentes idades consideradas variou, principalmente, em função das diferenças existentes entre as idades, também relacionadas às condições de manejo, como desbastes, desramas e densidade de indivíduos por hectare.

This study aimed to estimate biomass and organic carbon in stands of Pinus taeda L. at different ages (14, 16, 19, 21, 22, 23 and 32 years) and located in the municipality of General Carneiro (PR). In order to estimate biomass and organic carbon in different tree components (needles, live branches, dead branches, bark and stem wood), the destructive quantification method was used in which seven trees from each age category were randomly sampled across the stand. Stocks of biomass and organic carbon were found to vary between the different age categories, mainly as a result of existing dissimilarities between ages in association with forest management practices such as thinning, pruning and tree density per hectare.