ABSTRACT Purpose: To test the use of rainbow trout skin as a surgical mesh in abdominal hernioplasties in rats. Methods: The experiment involved 20 Wistar rats receiving implants of trout skin processed for disinfection in 0.5% glutaraldehyde and preserved in 100% glycerin. The animals were divided into four groups, divided at 7, 15, 30, and 90 days postoperatively. Clinical and infrared thermography evaluations were performed, and after euthanasia, assessments of adhesion formations and sample collection for histological evaluation were conducted. Results: The implant was observed to be intact, ensuring the integrity of the abdominal wall, support for the viscera, and normal mobility for the rats for up to 90 days. Low rates of clinical alterations were observed, with an intense inflammatory reaction up to day 7, chronic inflammation and the onset of angiogenesis at day 15, and a low inflammatory reaction with collagenous infiltrate and fibrosis at day 30. At day 90, the implants showed a collagenous and fibrotic infiltrate with a minimal inflammatory infiltrate. Conclusions: The surgical mesh of trout skin performed well, making it a potential alternative for surgical procedures in muscle aponeurotic corrections in the abdominal wall. Purpose Methods 2 05 0 5 0.5 100 glycerin groups 7 15 30 9 postoperatively euthanasia conducted Results intact wall viscera Conclusions well 0. 10 1 3

ABSTRACT Purpose: To histologically quantify the different tissues that make up the porcine ureter, (epithelial, connective, and muscular tissue) in the three segments labelled: cranial, middle and caudal, in order to identify the segment most compatible for use as a vascular graft. Methods: Fifteen porcine ureters were collected, divided into the three segments, and the samples were stained with hematoxylin and eosin, picrosirius red and Weigert’s resorcin-fuchsin. The immunohistochemistry technique was applied for alpha-smooth muscle actin. Collagen fibers, muscle, epithelium, and elastic fibers tissue were quantified, in the entire ureter, and divided into hemispheres, comparing the different segments. Results: When comparing hemisphere segments, significant differences were observed (p < 0.01) for collagen and muscle tissue, with the cranial segment presenting the greatest amount of these components when compared to the middle and caudal. No significant difference was observed between the segments when comparing the entire ureters. Conclusions: After comparing the segments by hemisphere, the cranial segment presented a slight advantage for use as a vascular graft due to presenting greater collagen fiber content. Purpose ureter epithelial, epithelial (epithelial connective labelled caudal Methods collected eosin Weigerts Weigert s resorcinfuchsin. resorcinfuchsin resorcin fuchsin. fuchsin resorcin-fuchsin alphasmooth alpha smooth actin epithelium quantified hemispheres Results p 0.01 001 0 01 Conclusions content 0.0 00 0.

O objetivo desta investigação foi caracterizar a morfometria do encéfalo e a formação do circuito arterial em equinos adultos mestiços de ambos os sexos. A fixação foi feita através de cânula plástica introduzida na artéria carótida comum com solução de formaldeído a 10%. Em seguida, foram feitas repleções vasculares com solução aquosa de Petrolátex S65 corado com pigmento Suvinil vermelho. Procedeu-se a craniotomia, remoção e morfometria dos encéfalos e dissecção para a observação das ramificações principais da artéria basilar e formação do circuito arterial. A média mais erro padrão da média do comprimento da artéria basilar foi de3,629 cm ± 0,1550 nos machos e 4,423 cm ± 0,1990 nas fêmeas (p=0,0037). Em todos os equinos dissecados, a artéria basilar originou-se da convergência das artérias vertebrais, emitindo a artéria cerebelar caudal, ramos variados para bulbo e ponte, ramos terminais e artérias cerebelares rostrais. As artérias da base do encéfalo estiveram na dependência dos sistemas carótico e vértebrobasilar. O circuito arterial do cérebro apresentou-se fechado rostral e caudalmente em 100% dos animais dissecados.

The aim of this study was to describe the main branches of the basilar artery, formation of the arterial circuit and the morphometry of the brain in adult crossbreed horses of both sexes. The setting was made by plastic tube inserted into the common carotid artery with 10% formaldehyde solution. After fixed, latex solution stained with pigment was injected. Craniotomy, morphometry of the brain and dissection were made to identify the main branches of the basilar arteries and the formation of the arterial circuit. The mean and standard error of the mean of the basilar artery was 3.629 cm ± 0.1550 in males and 4.423 cm ± 0.1990 in females (p=0.0037). In all animals the basilar artery originated from the fusion of the vertebral arteries giving rise to caudal cerebellar arteries, different numbers of branches to bulbus and pons, terminal branches and rostral cerebellar arteries. The arteries of the base of the brain depended on the carotid and vertebral-basilar systems. The brain circuit was closed rostrally and caudally in all horses.