Abstract Grape pomace (seed, skin and stem) is a winery byproduct with high levels of biologically active compounds, such as antioxidants and antimicrobials, that could be converted into high added-value products. Since these components are easily degraded by oxygen, light and high temperature exposure, stabilization is important, for instance, by a microencapsulation process. Therefore, the objective of this study was to investigate the influence on the particle characteristics of the operational conditions applied in the Supercritical Anti-Solvent (SAS) process for the co-precipitation of grape pomace extract and poly(-lactic-co-glycolic acid) (PLGA). The morphology and size of the particles formed, their stability and thermal profile were evaluated, and also the co-precipitation efficiency. The conditions studied allowed the production of microparticles with spherical shape for all operational conditions, with estimated particle size between 4 ± 2 and 11 ± 5 µm, and very good co-precipitation efficiencies (up to 94.4 ± 0.6%). The co-precipitated extract presented higher stability compared to the crude extract, indicating the effectiveness of the co-precipitation process and coating material against degradation processes.

Parboiled rice bran oil was obtained with supercritical CO2 at temperatures and pressures varying from 25 to 60ºC and from 150 to 250 bar, respectively. This study was divided into two different parts: initially, the experiments were carried out with one separation step. In the second part, experiments were performed with two separators in series. The temperatures and the pressures of the first separator were 25 and 40ºC, and 100 and 150 bar. The second separator was maintained at 2ºC and 25bar. This procedure results in the precipitation of rice bran oil with different concentrations of tocols in the first and second separators. The extracts obtained were analyzed by HPLC to verify the presence of tocopherols and tocotrienols. Unlike other vegetable oils, rice bran oil contains a larger amount of tocotrienols, specially gamma-tocotrienol, than of tocopherols. Finally the extraction curves were modeled by Sovová's method.