Abstract Obtaining transgenic sugarcane lines in vitro is a laborious, expensive, and time-consuming process. To minimize these disadvantages, the presence of transgenes must be checked as soon as possible. Here, we describe a method for direct PCR amplification of DNA from sugarcane plantlets, using smaller quantities of vegetal material, low-cost lab reagents, and rapid processing. By using this methodology, we were able to identify 100% of the transgenic lines generated. The outlined protocol enabled us to perform an earlier checking of the transgene insertions on genomes of transformed lines reducing costs and labor at in vitro stages. laborious expensive timeconsuming time consuming process disadvantages possible Here plantlets material lowcost low cost reagents processing methodology 100 generated stages 10 1

ABSTRACT This investigation has for objective to elaborate an orientation program for the therapeutic education of patient with arthritis rheumatoid in the hospital of the Ecuadorian Institute of Social security of the city of Babahoyo in the Republic of Ecuador. This investigation is part of a project of university linking of the career of medicine of the Autonomous Regional University of the Andes. Where they were evidenced a group of limitations that you/they ballast this thematic one in patient of the investigated city. He/she was carried out a pre-experiment with two moments one initial and another end, for the same one they were kept in mind statistical theoretical, empiric and mathematical methods. The results obtained after the application of the elaborated program are valid and satisfactory because a significant difference existed in them.

RESUMEN Esta investigación tiene por objetivo elaborar un programa de orientación para la educación terapéutica de pacientes con artritis reumatoide en el hospital del Instituto Ecuatoriano de Seguridad Social de la ciudad de Babahoyo en la República del Ecuador. Esta investigación es parte de un proyecto de vinculación universitaria de la carrera de medicina de la Universidad Regional Autónoma de los Andes. Donde se evidenciaron un grupo de limitaciones que lastran esta temática en pacientes de la ciudad investigada. Se realizó un pre experimento con dos momentos uno inicial y otro final, para el mismo se tuvieron en cuenta métodos teóricos, empíricos y matemáticos estadísticos. Los resultados obtenidos luego de la aplicación del programa elaborado son válidos y satisfactorios pues existió una diferencia significativa en ellos.

Objective: This study aimed to analyze the characteristics of a Hospital Home Care target population, its structure and competence. Methods: Retrospective descriptive study. Results: The results of this study indicated that 78,8% of the patients were more than 65 years old Chronic process represented 46,9% of the cases. Among the pathology causes group, 52,1% were included in the pluripathologies category, followed by tumors and neoplasies (28%) and by circulatory diseases (18,8%). The main admission way to Hospital Home Care was by the Short Staying Medical Unity (53%) and the main destination of the patients was to the primary attention (62%). Discussion: Aged people with chronic or terminal pluripathologies met an efficient strategy in Hospital Home Care, considering that due to their own complexities, they can surpass treatment possibilities in primary attention. In conclusion, there is hospital, which clearly is projected to patients and to sanitary assistance quality and effectiveness.

Objetivo: Analizar las características de la población diana de hospitalización domiciliaria (HD), su estructura y competencia. Método: Estudio descriptivo retrospectivo. Resultados: El 78,8% de los pacientes son mayores de 65 años. Los procesos crónicos representan el 46,9%. Para los grupos de causas patológicas, el 52,1% se incluyen en la categoría de otras pluripatologías, seguido de los tumores y neoplasias (28%) y las enfermedades del aparato circulatorio (18,8%). La principal puerta de entrada en HD fue la UMCE (53,0%) y el principal destino de los pacientes fue la atención primaria (62,0%). Discusión: Los ancianos con plurupatologias crónicas o terminales tienen en la HD una estrategia eficiente, ya que son pacientes que por su complejidad pueden superar las posibilidades de tratamiento en atención primaria. Se concluye que hay un problema de comunicación entre la atención primaria y el hospital, que obviamente se proyecta hacia los pacientes y hacia la calidad y efectividad de la asistencia sanitaria.