RESUMO. Este trabalho teve por objetivo avaliar as características físico-químicas do leite de cabras suplementadas com teores de Nutrientes Digestíveis Totais (NDT) no período seco do ano. Foram utilizadas amostras de leite de 20 cabras as quais foram divididas em quatro grupos, sendo que três grupos receberam suplementação de 400 g de concentrado por animal com diferentes teores de NDT (65, 75 ou 85%) e o grupo controle que não recebeu concentrado. Foram utilizadas quatro amostras de leite para cada animal, em quatros períodos de coleta, as quais foram submetidas à análise físico-química de acidez titulável, crioscopia e gordura. Não houve diferença entre os tratamentos para os parâmetros de acidez e gordura. As amostras de leite dos animais que receberam a dieta sem concentrado apresentaram maior valor para o índice crioscópico. As médias das amostras dos quatro tratamentos estavam dentro dos parâmetros fixados pela legislação brasileira para todos os parâmetros avaliados. A utilização de concentrado contendo teor de 85% de NDT na suplementação de cabras favoreceu as características físico-químicas do leite durante o período seco.

ABSTRACT. We evaluated the physical and chemical characteristics of raw milk from goats supplemented with levels of Total Digestible Nutrients (TDN) in the dry period. Samples from 20 goats were divided into 4 groups, 3 groups received 400g concentrate per animal with different levels of TDN (65, 75 and 85%), and the control group (without concentrate). Four samples of milk were used for each animal, in four sampling periods, which were subjected to physical and chemical analysis. There was no significant difference between the treatments for the parameters of acidity and fat. The diet without concentrate presented higher value for the cryoscopic index. Mean values of the four treatments were within the range set by the Brazilian legislation for all parameters evaluated. The use of concentrate, containing 85% TDN in goat supplementation, was favored the physical and chemical characteristics of the milk during the dry period.

To investigate microbial diversity and identify spoilage bacteria in fresh pork sausages during storage, twelve industrial pork sausages of different trademarks were stored at 4 ºC for 0, 14, 28 and 42 days, 80% relative humidity and packaged in sterile plastic bags. Microbiological analysis was performed. The pH and water activity (a w) were measured. The culture-independent method performed was the Polymerase Chain Reaction - Denaturing Gradient Gel Electrophoresis (PCR-DGGE). The culture-dependent method showed that the populations of mesophilic bacteria and Lactic Acid Bacteria (LAB) increased linearly over storage time. At the end of the storage time, the average population of microorganisms was detected, in general, at the level of 5 log cfu g-1. A significant (P < 0.005) increase was observed in pH and a w values at the end of the storage time. The PCR-DGGE allowed a rapid identification of dominant communities present in sausages. PCR-DGGE discriminated 15 species and seven genera of bacteria that frequently constitute the microbiota in sausage products. The most frequent spoilage bacteria identified in the sausages were Lactobacillus sakei and Brochothrix thermosphacta. The identification of dominant communities present in fresh pork sausages can help in the choice of the most effective preservation method for extending the product shelf-life.