Abstract The influence of refrigeration on the post-embryonic development of Chrysomya putoria larvae was evaluated, regarding its resistance in the logistics of storage and distribution in biotherapy. Previously sterilized larvae were submitted to four periods of storage under refrigeration (T1=12 h, T2=24 h, T3=48 h and T4=72 h) and control (without sterilization and refrigeration). Newly hatched larvae (0.200 g) were stored between 3 and 9ºC. After refrigeration, 40 neo-larvae (in triplicate) were transferred to 50 g of protein diet and incubated in an acclimatized chamber. There was a significant difference in the larval body mass (T1 and T2) and in the duration of larval, pupal and total development (T3 and T4). The sex ratios found in the four treatments did not differ from what was expected. Normality rates were 100% for all treatments. There was no significant difference between the Control, T1 and T2 treatments for larval, pupal and total viability. There was a significant difference between control (C) and T4 (larval viability), between C, T3 and T4 (pupa) and between C and T4 (total). C. putoria has resistance under refrigeration and storage of up to 56 h, presenting viability above 70% for use in biotherapy.

Nós avaliamos aqui os efeitos de diferentes concen trações do antibiótico ampicilina sobre o crescimento e desenvolvimento de Chrysomya putoria. Larvas de primeiro instar (L1), de terceira geração, criadas em 60 gramas de homogenato de moela em agar 65% foram tratadas com ampicilina sódica. O experimento consistiu de quatro repetições (40 larvas / réplicas) para cada concentração do antibiótico testado (T1: 66µg/mL; T2: 81,33µg/mL e T3: 166,66µg/mL) e o T4: controle. A massa corporal das larvas maduras, após abandonarem a dieta, foi registrada em lotes de cinco. A variação entre a massa corporal média de larvas e a duração das taxas de desenvolvimento de larva, pupa e a duração total do desenvolvimento, viabilidades e taxas de normalidade foram analisadas por ANOVA. Não houve diferença significativa entre os quatro tratamentos nos seguintes parâmetros: massa corporal de larvas que abandonaram a dieta, bem como, a duração larval, pupal e desenvolvimento total. As proporções sexuais encontradas nos quatro tratamentos não diferiram do esperado. Taxas de normalidade foram de 100% para todos tratamentos. Não houve diferença significativa entre os tratamentos para as viabilidade larval e total, mas a viabilidade de pupa diferiu significativamente entre T1 e o controle, T1 e T2, e entre o controle e T3. O antibiótico pareceu não alterar significativamente o desenvolvimento de C. putoria.

Here we evaluate the effects of different concentrations of the antibiotic ampicillin on the growth and development of Chrysomya putoria. Third-generation, first instar larvae (L1) reared on 60 grams of homogenate+agar 65% were treated with ampicillin sodium. The experiment consisted of four replicates (40 larvae/replicate) of each antibiotic concentration tested (T1: 466µg/mL ; T2: 81.33 mg/mL and T3: 166.66mg/mL) and a T4: control. The body mass of the mature larvae, after they abandoned the diet, were recorded in batches of five. The variation between the mean body mass of larvae and the duration of larval and pupal stages, and overall duration of the development, viability and normal rates were analyzed by ANOVA. There were no significant differences between the four treatments in the following parameters: body mass of larvae that discontinued the diet as well as the duration of larval, pupal, and total development. The sex ratios found in the four treatments did not differ from those expected. Normality rates were 100% for all treatments. There were no significant differences between treatments for larval and overall viability, but pupal viability differed significantly between T1 and the control, T1 and T2, and between the control and T3. The antibiotic did not appear to significantly alter the development of C. putoria.