ABSTRACT INTRODUCTION In 2019, a new coronavirus emerged causing a global pandemic. During 2020, vaccines with acceptable safety and efficacy were developed to reduce complications and deaths. The present work set out to investigate the relationship between vaccination and contagion between partners. METHODS Data from the Federal Register of Nominalized Vaccination and confirmed cases in the province of Santa Fe registered in the Argentine Integrated Health Information System from January 1 to June 30, 2021 in people aged 18 to 65 years were analyzed. 5,291 pairs of an index case and a secondary case were constituted, whose addresses coincided and whose dates of onset of symptoms were in a range of 2 to 14 days. Pairs were selected in which one person was vaccinated and the other was not, with a total of 494 pairs. RESULTS The average age of the index cases was 40.8 years and that of the secondary cases was 40.5 years. There were 234 vaccinated people among the index cases and 386 among the secondary ones. Of the 494 pairs with one vaccinated and one unvaccinated person, the index case was the vaccinated person in 179 pairs, and in 315, the index was the unvaccinated person. DISCUSSION The analysis suggests that, in home infections, where vaccinated and unvaccinated people are involved, it is more common for the unvaccinated person to be the index case. This points to the importance of vaccinating the partners of people with risk factors.

RESUMEN INTRODUCCIÓN En 2019, surgió un nuevo coronavirus que causó una pandemia mundial. Durante 2020, se desarrollaron vacunas con aceptable seguridad y eficacia para disminuir complicaciones y muertes. El presente trabajo se propuso investigar la relación entre la vacunación y el contagio entre convivientes. MÉTODOS Se analizaron datos del Registro Federal de Vacunación Nominalizado y los casos confirmados en provincia de Santa Fe registrados en el Sistema Integrado de Información Sanitaria Argentina desde 1 de enero hasta 30 de junio de 2021 en personas de 18 a 65 años. Se constituyeron 5291 pares de un caso índice y un caso secundario, cuyos domicilios coincidían y cuyas fechas de inicio de síntomas se hallaban en un rango de 2 a 14 días. Se seleccionaron los pares en los que una persona estaba vacunada y la otra no, con un total de 494 pares. RESULTADOS El promedio de edad de los casos índice fue de 40,8 años y el de los secundarios fue de 40,5 años. Se hallaron 234 personas vacunadas entre los casos índice y 386 entre los secundarios. De los 494 pares con una persona vacunada y una no vacunada, el caso índice fue la persona vacunada en 179 pares, y en 315 pares el índice fue la persona no vacunada. DISCUSIÓN El análisis sugiere que, en los contagios intradomiciliarios, donde se involucran personas vacunadas y no vacunadas, es más frecuente que sea la persona no vacunada quien constituya el caso índice. Esto señala la importancia de vacunar a los convivientes de las personas con factores de riesgo.

We present here three expression plasmids for Trypanosoma cruzi adapted to the Gateway® recombination cloning system. Two of these plasmids were designed to express trypanosomal proteins fused to a double tag for tandem affinity purification (TAPtag). The TAPtag and Gateway® cassette were introduced into an episomal (pTEX) and an integrative (pTREX) plasmid. Both plasmids were assayed by introducing green fluorescent protein (GFP) by recombination and the integrity of the double-tagged protein was determined by western blotting and immunofluorescence microscopy. The third Gateway adapted vector assayed was the inducible pTcINDEX. When tested with GFP, pTcINDEX-GW showed a good response to tetracycline, being less leaky than its precursor (pTcINDEX).

Here we present a one-tube nested PCR test, which allows the detection of minimal quantities of Chlamydia trachomatis in human fluids. This assay includes the use of an internal control to avoid false negative results due to the presence of inhibitors. The results obtained show that this assay is robust enough to be used for clinical diagnosis.