NLRP1, the first identified inflammasome-forming sensor, is thought to be involved in cancer, yet its definite function in lung adenocarcinoma (LUAD) remains unclear. Herein, we explored the expression and function of NLRP1 in LUAD. Decreased NLRP1 expression was identified in LUAD, which was associated with a poor prognosis. Overexpression of NLRP1 inhibited tumor growth in vitro and in vivo. Mechanically, this effect was observed regardless of inflammasome activation. Further studies revealed that overexpression of NLRP1 downregulated the phosphorylation of DRP1 and promoted mitochondrial fusion, which was mediated by inhibition of NF-κB activity. NF-κB agonist could neutralize the effect of NLRP1 on mitochondrial dynamics. In addition, LUAD sensitivity to cisplatin was enhanced by decreased mitochondrial fission resulting from up-regulated NLRP1. In conclusion, our findings demonstrated an unexpected role of NLRP1 in LUAD by modulating mitochondrial activities, which provides strong evidence for its potential in LUAD treatment. NLRP inflammasomeforming forming sensor cancer (LUAD unclear Herein prognosis vivo Mechanically activation DRP fusion NFκB NF κB activity dynamics addition upregulated up regulated conclusion activities treatment

Sepsis is a systemic inflammatory response syndrome in which the host response to infection is dysregulated, leading to circulatory dysfunction and multi-organ damage. It has a high mortality rate and its incidence is increasing year by year, posing a serious threat to human life and health. Mesenchymal stem cells (MSC) have the following properties: hematopoietic support, provision of nutrients, activation of endogenous stem/progenitor cells, repair of tissue damage, elimination of inflammation, immunomodulation, promotion of neovascularization, chemotaxis and migration, anti-apoptosis, anti-oxidation, anti-fibrosis, homing, and many other effects. A large number of studies have confirmed that MSC from different sources have their own characteristics. This article reviews the pathogenesis of sepsis, the biological properties of MSC, and the advantages and disadvantages of different sources of MSC for the treatment of sepsis and their characteristics. dysregulated multiorgan multi organ damage health (MSC support nutrients stemprogenitor progenitor inflammation immunomodulation neovascularization migration antiapoptosis, antiapoptosis anti apoptosis, apoptosis anti-apoptosis antioxidation, antioxidation oxidation, oxidation anti-oxidation antifibrosis, antifibrosis fibrosis, fibrosis anti-fibrosis homing effects characteristics

Resumen Antecedentes: La enfermedad de injerto contra huésped (EICH) es una complicación importante después del trasplante alogénico de células madre hematopoyéticas. Objetivos: Para dilucidar el papel de TLR4, el principal receptor de LPS bacteriano, en el desarrollo de GVHD, construimos un modelo de GVHD en ratones knockout para TLR4 (TLR4-/-) y monitoreamos el quimerismo celular. Métodos: En este estudio, usamos PCR para identificar si se establecieron ratones knockout para TLR4 (TLR4-/-). Antes del trasplante, pretratamos a los ratones con irradiación para obtener la dosis de irradiación adecuada. Se aplicó citometría de flujo para medir el estado de quimerismo, las distribuciones de APC y células T en ratones receptores TLR4+/+ y TLR4-/-. Resultados: El estado general de los receptores de TLR4-/- fue mejor que el de los receptores de TLR4+/+, y los ratones receptores de TLR4-/- mostraron manifestaciones de GVHD menos graves que los ratones receptores de TLR4+/+. La mayoría de las APC y las células T en el bazo del ratón huésped se derivaron de las células del donante, y las células T CD4+, incluidas las células T de memoria, se encontraban en su mayoría en los ratones huéspedes. Conclusión: En general, nuestros datos muestran que la eliminación de TLR4 atenuó el desarrollo de GVHD, lo que sugiere que TLR4 podría usarse como un nuevo objetivo y paradigma terapéutico en las terapias de GVHD.

Abstract Background: Graft-versus-host disease (GVHD) is a major complication after allogeneic hematopoietic stem cell transplantation. Objective: To elucidate the role of Toll-like receptor 4 (TLR4), the major receptor for bacterial lipopolysaccharide, in the development of GVHD, we constructed a GVHD model in TLR4 knockout (TLR4-/-) mice and monitored the cell chimerism. Methods: In this study, we used polymerase chain reaction to identify whether TLR4 knockout (TLR4-/-) mice were established. Before transplantation, we pretreated mice with irradiation so as to obtain an appropriate irradiation dose. Flow cytometry was applied to measure the chimerism status, the distributions of antigen-presenting cells (APCs), and T-cells in TLR4+/+ and TLR4-/- recipient mice. Results: The general condition of TLR4-/- recipients was better than that of TLR4+/+ recipients, and the TLR4-/- recipient mice showed less severe GVHD manifestations than the TLR4+/+ recipient mice. Most of the APCs and T-cells in the host mouse spleen were derived from donor cells, and CD4+ T-cells, including memory T-cells, were in the majority in host mice. Conclusion: In general, our data show that TLR4 deletion attenuated GVHD development, which suggests that TLR4 could be used as a novel target and therapeutic paradigm in GVHD therapies.

RESUMO Introdução O desenvolvimento do nível técnico do tênis de mesa e a reforma das regras, requerem que os jogadores de tênis de mesa tenham uma base técnica proporcionalmente abrangente, visando escalar o topo do tênis de mesa mundial. Objetivo Estudar o efeito da competição de alta intensidade sobre a aptidão física dos jogadores de tênis de mesa. Métodos Ao analisar os questionários de treinadores e atletas nas faculdades e universidades da província de Zhejiang, bem como o estado de desenvolvimento e a situação dos soldados e equipes de soldados nas faculdades e universidades da província de Zhejiang, descobrir os fatores que restringem o nível competitivo do tênis de mesa nas faculdades e universidades comuns de nossa província. Resultados A maioria dos atletas sentiu que as qualidades específicas de agilidade e força eram importantes, representando 63% do total, enquanto a resistência e a velocidade eram menos importantes, representando 19% e 18% respectivamente. Conclusão O tempo dedicado ao treinamento físico é breve, e a disposição do tempo de treinamento é insuficiente. Em comparação com as equipes esportivas de alto nível, a qualidade da preparação física e o tempo de treinamento técnico particular não podem ser combinados de forma racional e satisfatória. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

ABSTRACT Introduction The development of the technical level of table tennis and the reform of the rules require players to have a proportionally comprehensive technical base, aiming to climb to the top of world table tennis. Objective Study the effect of high-intensity competition on the physical fitness of table tennis players. Methods By analyzing the questionnaires of coaches and athletes in colleges and universities in Zhejiang province, as well as the development status and situation of soldiers and soldier teams in colleges and universities in Zhejiang province, find out the factors that restrict the competitive level of table tennis in ordinary colleges and universities in our province. Results Most athletes felt that the specific qualities of agility and strength were important, accounting for 63% of the total, while endurance and speed were less important, accounting for 19% and 18%, respectively. Conclusion The time devoted to physical training is short, and the disposition of training time is insufficient. Compared to high-level sports teams, the quality of physical preparation and the particular technical training time cannot be combined rationally and satisfactorily. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMEN Introducción El desarrollo del nivel técnico del tenis de mesa y la reforma de las reglas, requieren que los jugadores de tenis de mesa tengan una base técnica proporcionalmente amplia, con el objetivo de subir a la cima del tenis de mesa mundial. Objetivo Estudiar el efecto de la competición de alta intensidad sobre la aptitud física de los jugadores de tenis de mesa. Métodos Mediante el análisis de los cuestionarios de los entrenadores y atletas de los colegios y universidades de la provincia de Zhejiang, así como del estado de desarrollo y la situación de los equipos de soldados y soldados de los colegios y universidades de la provincia de Zhejiang, averiguar los factores que restringen el nivel competitivo del tenis de mesa en los colegios y universidades ordinarios de nuestra provincia. Resultados La mayoría de los atletas consideraron que las cualidades específicas de agilidad y fuerza eran importantes, con un 63% del total, mientras que la resistencia y la velocidad eran menos importantes, con un 19% y un 18% respectivamente. Conclusión El tiempo dedicado al entrenamiento físico es breve, y la disposición del tiempo de entrenamiento es insuficiente. En comparación con los equipos deportivos de alto nivel, la calidad de la preparación física y el tiempo de entrenamiento técnico particular no pueden combinarse de forma racional y satisfactoria. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Resumen Objetivo Evaluar los efectos de la intervención terapéutica de enfermería con base en el modelo de trayectoria de la enfermedad crónica en la ansiedad y en la calidad de vida de pacientes con enfermedad arterial coronaria (EAC). Métodos Un total de 118 pacientes con EAC admitidos entre febrero de 2019 y febrero de 2021 fueron distribuidos aleatoriamente en grupos control y observación (n = 59). El grupo control recibió intervención de enfermería de rutina, mientras el grupo observación recibió intervención con base en el modelo de trayectoria de la enfermedad crónica. Se compararon los síntomas clínicos, la escala de autoevaluación de ansiedad (EAA), la calidad de vida (CV) y el puntaje de autoeficacia. Se compararon las tasas de incidencia de complicaciones. El análisis de regresión lineal multivariado se realizó para el efecto mediador de la autoeficacia en la relación entre ansiedad y CV. Se elaboró y validó un modelo de ecuación estructural. Resultados Después de la intervención, los síntomas clínicos y el puntuaje de la EAA disminuyeron de forma considerable en ambos grupos, especialmente en el grupo observación (P<0,05). Los puntajes de CV y de autoeficacia aumentaron considerablemente en ambos grupos, particularmente en el grupo de observación (P<0,05). El puntaje de autoeficacia se correlacionó negativamente con el puntaje EAA y positivamente con el puntaje CV. Hubo una correlación negativa entre el puntaje EAA y el puntaje CV (P<0,05). El modelo de autoeficacia, ansiedad y CV presentó una buena adecuación y el efecto mediador de la autoeficacia en la relación entre ansiedad y CV fue de 0,896. La tasa de incidencia de complicaciones fue considerablemente inferior en el grupo observación que en el grupo control (P <0,05). Conclusión La intervención de enfermería con base en el modelo de trayectoria de la enfermedad crónica alivia de forma considerable la ansiedad, mejora la CV y aumenta el puntaje de autoeficacia de pacientes con EAC. La autoeficacia es un mediador de la relación entre ansiedad y CV. . (EAC) 11 201 202 n 59. 59 59) rutina EAA, , (EAA) (CV estructural P<0,05. P005 P P<0,05 0 05 (P<0,05) 0896 896 0,896 <0,05. 005 <0,05 <0,05) (EAC 1 20 5 (EAA P00 P<0,0 (P<0,05 089 89 0,89 00 <0,0 2 P0 P<0, (P<0,0 08 8 0,8 <0, P<0 (P<0, 0, <0 P< (P<0 < (P<

Resumo Objetivo Avaliar os efeitos da intervenção terapêutica de enfermagem baseada no modelo de trajetória da doença crônica na ansiedade e na qualidade de vida de pacientes com doença cardíaca coronária (DCC). Métodos Um total de 118 pacientes com DCC admitidos entre fevereiro de 2019 e fevereiro de 2021 foram distribuídos aleatoriamente em grupos controle e observação (n = 59). O grupo controle recebeu intervenção de enfermagem de rotina, enquanto o grupo observação recebeu intervenção com base no modelo de trajetória da doença crônica. Os sintomas clínicos, a escala de autoavaliação de ansiedade (SAS), a qualidade de vida (QV) e as pontuações de autoeficácia foram comparados. As taxas de incidência de complicações foram comparadas. A análise de regressão linear multivariada foi realizada para o efeito mediador da autoeficácia na relação entre ansiedade e QV. Um modelo de equação estrutural foi construído e validado. Resultados Após a intervenção, os sintomas clínicos e a pontuação da SAS diminuíram significativamente em ambos os grupos, especialmente no grupo observação (P<0,05). As pontuações de QV e autoeficácia aumentaram significativamente em ambos os grupos, particularmente no grupo de observação (P<0,05). A pontuação de autoeficácia correlacionou-se negativamente com a pontuação SAS e positivamente com a pontuação QV. Houve correlação negativa entre a pontuação SAS e a pontuação QV (P<0,05). O modelo de autoeficácia, ansiedade e QV apresentou boa adequação, e o efeito mediador da autoeficácia na relação entre ansiedade e QV foi de 0,896. A taxa de incidência de complicações foi significativamente menor no grupo observação do que no grupo controle (P <0,05). Conclusão A intervenção de enfermagem baseada no modelo de trajetória da doença crônica alivia significativamente a ansiedade, melhora a QV e aumenta a pontuação de autoeficácia de pacientes com DCC. A autoeficácia é um mediador da relação entre ansiedade e QV. . (DCC) 11 201 202 n 59. 59 59) rotina SAS, , (SAS) (QV comparados comparadas validado P<0,05. P005 P P<0,05 0 05 (P<0,05) correlacionouse correlacionou se adequação 0896 896 0,896 <0,05. 005 <0,05 <0,05) (DCC 1 20 5 (SAS P00 P<0,0 (P<0,05 089 89 0,89 00 <0,0 2 P0 P<0, (P<0,0 08 8 0,8 <0, P<0 (P<0, 0, <0 P< (P<0 < (P<

Abstract Objective We aimed to assess the effects of nursing intervention therapy based on chronic disease trajectory model on anxiety and quality of life (QOL) of patients with coronary heart disease (CHD). Methods A total of 118 CHD patients admitted from February 2019 to February 2021 were randomly assigned into control and observation groups (n=59). Control group was given routine nursing intervention, while observation group was given intervention based on chronic disease trajectory model. Clinical symptom, self-rating anxiety scale (SAS), QOL and self-efficacy scores were compared. Incidence rates of complications were compared. Multivariate linear regression analysis was performed for the mediating effect of self-efficacy on relationship between anxiety and QOL. A structural equation model was constructed and verified. Results After intervention, clinical symptom and SAS scores significantly declined in both groups, especially in observation group (P<0.05). QOL and self-efficacy scores rose significantly in both groups, particularly in observation group (P<0.05). Self-efficacy score was negatively correlated with SAS score and positively correlated with QOL score, and there was a negative correlation between SAS score and QOL score (P<0.05). The model of self-efficacy, anxiety and QOL had good fitness, and the mediating effect of self-efficacy on relationship between anxiety and QOL was 0.896. The incidence rate of complications was significantly lower in observation group than in control group (P<0.05). Conclusion Nursing intervention based on chronic disease trajectory model significantly relieves the anxiety, improves QOL, and increases the self-efficacy score of CHD patients. Self-efficacy is a mediator for the relationship between anxiety and QOL. (QOL CHD. . (CHD) 11 201 202 n=59. n59 n n=59 59 (n=59) selfrating self rating SAS, , (SAS) selfefficacy efficacy compared verified P<0.05. P005 P P<0.05 0 05 (P<0.05) Selfefficacy Self selfefficacy, efficacy, fitness 0896 896 0.896 (CHD 1 20 n5 n=5 5 (n=59 (SAS P00 P<0.0 (P<0.05 089 89 0.89 2 n= (n=5 P0 P<0. (P<0.0 08 8 0.8 (n= P<0 (P<0. 0. (n P< (P<0 (P< (P

Resumo Fundamento A trombose completa da falsa luz facilita a remodelação da dissecção aórtica tipo B (DATB). As características morfológicas afetam a trombose na falsa luz. Objetivos Discutir os fatores pré-admissão presentes, que influenciam a trombose da falsa luz em pacientes com DATB. Metodologia Ao todo, 282 pacientes diagnosticados com DATB em nosso hospital foram estudados, no período entre janeiro de 2008 e dezembro de 2017. Os indivíduos foram divididos em um grupo trombótico e um grupo não trombótico, com base na detecção de qualquer trombo na falsa luz. Analisamos as diferenças entre os dois grupos com relação aos dados clínicos, o comprimento vertical da dissecção e o diâmetro da aorta. Valores de p < 0,05 foram considerados estatisticamente diferentes de modo significativo. Resultados Diferenças significativas entre o grupo trombótico e o grupo não trombótico foram encontradas com relação à idade (53,92 ± 11,40 vs. 50,36 ± 10,71, p = 0,009) e proporção de pacientes com insuficiência renal (7,83% vs. 16,38%, p = 0,026). Nas zonas 3–9, o diâmetro da luz verdadeira do grupo trombótico foi significativamente maior do que no grupo não trombótico (p < 0,05). A análise de regressão logística binária mostrou que o diâmetro da luz verdadeira na zona 5 e a insuficiência renal foram preditores independentes de trombose da falsa luz. Conclusões A idade e a função renal estiveram associadas à trombose na falsa luz. Potencialmente, a diferença entre o diâmetro da luz verdadeira e o da falsa luz pode influenciar na trombose da falsa luz. . (DATB) préadmissão pré admissão presentes todo 28 estudados 200 2017 clínicos aorta 005 0 05 0,0 significativo 53,92 5392 53 92 (53,9 1140 11 40 11,4 vs 5036 50 36 50,3 1071 10 71 10,71 0,009 0009 009 7,83% 783 7 83 (7,83 1638 16 38 16,38% 0,026. 0026 0,026 026 0,026) 39 3 9 3–9 0,05. 0,05) Potencialmente (DATB 2 20 201 00 0, 53,9 539 (53, 114 1 4 11, 503 50, 107 10,7 0,00 000 7,83 78 8 (7,8 163 16,38 002 0,02 02 3– 53, (53 10, 7,8 (7, 16,3 (5 7, (7 16, (

Abstract Background Complete thrombosis of the false lumen facilitates remodeling of type B aortic dissection (TBAD). Morphological characteristics affect thrombosis in the false lumen. Objectives Discuss the factors present before admission that influence false lumen thrombosis in patients with TBAD. Methods We studied 282 patients diagnosed with TBAD in our hospital between January 2008 and December 2017. We divided the subjects into a thrombotic group and a non-thrombotic group based on whether any thrombus was detectable in the false lumen. We analyzed the differences between the two groups with respect to clinical data, the vertical length of the dissection, and the diameter of the aorta. P values < 0.05 were considered statistically significantly different. Results Significant differences between the thrombotic group and non-thrombotic group were found with respect to age (53.92 ± 11.40 vs. 50.36 ± 10.71, p = 0.009) and proportion of patients with renal insufficiency (7.83% vs. 16.38%, p = 0.026). In zones 3–9, the true lumen diameter of the thrombotic group was significantly larger than in the non-thrombotic group (p < 0.05). Binary logistic regression analysis showed that true lumen diameter in zone 5 and renal insufficiency were independent predictors of false lumen thrombosis. Conclusions Age and renal function were associated with thrombosis in the false lumen. Potentially, the difference between the diameter of the true lumen diameter and that of the false lumen may influence the thrombosis of the false lumen. . (TBAD) 28 200 2017 nonthrombotic non data aorta 005 0 05 0.0 different 53.92 5392 53 92 (53.9 1140 11 40 11.4 vs 5036 50 36 50.3 1071 10 71 10.71 0.009 0009 009 7.83% 783 7 83 (7.83 1638 16 38 16.38% 0.026. 0026 0.026 026 0.026) 39 3 9 3–9 0.05. 0.05) Potentially (TBAD 2 20 201 00 0. 53.9 539 (53. 114 1 4 11. 503 50. 107 10.7 0.00 000 7.83 78 8 (7.8 163 16.38 002 0.02 02 3– 53. (53 10. 7.8 (7. 16.3 (5 7. (7 16. (

Abstract Pulsed Electric field (PEF) cell lysis technology, as a new technology, has been widely used in the fields of food sterilization. Nowadays, there are still several problems in PEF cell lysis: low cell lysis rate, high temperature rise, the presence of low velocity regions, etc. In this study, COMSOL Multiphysics was used to conduct simulation research, and the electric field, flow field and temperature field of plate treatment chamber, coaxial treatment chamber and co-field treatment chamber were coupled to simulate, and the advantages and disadvantages of the three classical treatment chamber structures were analyzed. In addition, this paper proposes a new structure of the co-field treatment chamber. Through the comparison and simulation of the coupling between our design and other four structures, the electric field intensity of our design treatment area is 20.03 kV/m, which is 46.2% higher than the highest of others. The temperature rise within 1s is 0.93 K, which is 2/3 lower than others. Our design almost eliminates the peak electric field area at the junction between the inside of the electrode and the insulator, and the maximum electric field intensity in this area is reduced by 24.4% compared with others. (PEF technology sterilization Nowadays rate regions etc study research cofield co simulate analyzed addition 2003 20 03 20.0 kVm kV m kV/m 462 46 2 46.2 others s 093 0 93 0.9 K 23 3 2/ insulator 244 24 4 24.4 200 20. 46. 09 9 0. 24.

Abstract A reinforced concrete (RC) column improved with central large-diameter butted steel rods (CLBSR column) has been proposed to increase the limit value of the axial compression ratio. This paper presents the seismic behavior analysis of CLBSR columns subjected to low-cycle reciprocating loading. The CLBSR columns were also compared with the RC column improved with central large-diameter steel rods (CLSR column) and the RC column improved with central reinforcement bars (CRB column) under the same experimental conditions. The typical failure modes, hysteretic curves, skeleton curves, stiffness degradation, and energy dissipation capacity were all investigated. The improved PARK damage model obtained the corresponding relationships between the damage indices and the damage state of each column. The test results indicated that the CLBSR columns and CLSR columns had similar seismic behavior, but were slightly inferior to the CRB columns. Overall, the butt joint of large-diameter steel rods had little effect on the seismic behavior.

ABSTRACT Purpose: This study investigated the effects of oral administration of Clostridium butyricum (C. butyricum) on inflammation, oxidative stress, and gut flora in rats with hepatic ischemia reperfusion injury (HIRI). Methods: The rats from C. butyricum group were given C. butyricum for 5 days. Then, hepatic ischemia for 30 min and reperfusion for 6 h were performed in all the rats. After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed. Results: The rats given C. butyricum showed decreased ALT, AST, LPS, and MDA; improved GSH and histological damage; changes in SCFAs; declined TNF-α, IL-6, TLR4, and pNF-κBp65/NF-κBp65; and changes in the gut microbial composition, which decreased the Firmicutes/Bacteroidetes ratio and increased the relative abundance (RA) of probiotics. Conclusions: C. butyricum supplementation protected against HIRI by regulating gut microbial composition, which contributed to the decreased LPS and attenuation of inflammation and oxidative stress. These indicate C. butyricum may be a potent clinical preoperative dietary supplement for HIRI.

Abstract Character strengths could effectively prevent negative psychological outcomes in adults. However, there was little research conducted among early adolescents. The present study aimed to explore character strengths that were independently related to fewer behavior problems in early adolescents. In total, 521 early adolescents (mean age 10.92 ± 0.04, range 10–12 years) were recruited from primary schools in Sichuan, China. Character strengths were measured using the Values in Action Inventory of Strengths for Youth (VIA-Youth). Behavior problems were measured using the Conners Parent Symptom Questionnaire (PSQ). The results showed that behavior problems were negatively correlated with character strengths ( r = −0.14 to −0.3, p < 0.05 Bonferroni corrected). Character strengths explained a significant proportion of additional variance (14–22%) in five types of behavior problems after controlling the effect of demographic factors (residence, left-behind experiences, maternal education level). Moreover, several specific character strengths showed an independent contribution ( β = −0.34 to −0.14 for self-regulation, perseverance, zest, humility, and leadership; β = 0.21 to 0.34, for hope; all p < 0.05) to behavior problems. Our study revealed that character strengths were protective factors against behavior problems in early adolescents.

Abstract Objective: To investigate the impact of recombinant human interferon α1b (rhIFNα1b) treatment in infants hospitalized with lower respiratory tract infections on subsequent wheezing. Methods: The clinical data of infants (n = 540) with viral pneumonia, wheezy bronchitis, or bronchiolitis hospitalized in 19 Chinese hospitals from June 2009 to June 2015 were retrospectively analyzed. The parameters relevant to wheezing episodes within the last year were collected by telephone and questionnaires. The rhIFNα1b treatment group (n = 253) and control group (n = 287) were compared in terms of wheezing episodes within the last year. Moreover, the wheezing group (95 cases) and non-wheezing group (445 cases) were compared. Results: Out of 540 cases, 95 (17.6%) experienced wheezing episodes, 13.8% (35/253) cases treated with rhIFNα1b, and 20.9% (60/287) cases without rhIFNα1b experienced wheezing episodes within the last year. The rhIFNα1b treatment significantly improved wheezing episodes within the last year, compared with the control peers (p = 0.031). Single-factor regression showed statistically significant differences between the wheezing and non-wheezing groups in terms of age, rhIFNα1b use, childhood and family history of allergy, housing situation, and feeding history (p < 0.05). Binary logistic regression showed a childhood history of allergy (OR = 2.14, p = 0.004), no rhIFNα1b use (OR = 1.70, p = 0.028), and living in a crowded house (OR = 1.92, p = 0.012) might be risk factors of subsequent wheezing. Accordingly, breastfeeding (OR = 0.44, p = 0.008) and hospitalization age of 1-year-old (OR = 0.58, p = 0.024) were protective factors. Conclusions: Early use of rhIFNα1b in infants hospitalized with lower respiratory tract infections and breastfeeding could prevent subsequent wheezing. Living in a crowded house could promote subsequent wheezing.

Resumo Introdução: Evidências emergentes indicam que as condições fisiológicas e patológicas do nariz são reguladas pós-transcripcionalmente por microRNAs, uma classe de pequenos RNAs não codificantes. Recentemente, o microRNA-223-3p tem sido cada vez mais implicado na modulação da rinite alérgica. Objetivo: Avaliar o papel e o mecanismo do microRNA-223-3p em um modelo de rinite alérgica em camundongos. Método: O nível de expressão do microRNA-223-3p foi medido no soro de 41 pacientes com rinite alérgica e 39 controles saudáveis com polimerase chain reaction quantitativo em tempo real. Camundongos BALB/c foram usados para estabelecer um modelo de rinite alérgica através de sensibilização intraperitoneal e teste de provocação nasal com ovalbumina. O microRNA-223-3p agomir/antagomir foi então administrado por via intranasal a camundongos após provocação com ovabulmina por mais uma semana. Os sintomas de prurido nasal e espirros foram registrados. A concentração sérica de imunoglobulina E específica para ovalbumina, expressão de microRNA-223-3p e níveis de citocinas pró-inflamatórias (IL-4, IL-5, IFN-γ) na mucosa nasal foram medidos por ELISA e polimerase chain reaction quantitativo, respectivamente. As alterações histopatológicas foram avaliadas com coloração com hematoxilina e eosina. Resultados: Os níveis de microRNA-223-3p aumentaram significativamente em ambos os pacientes e camundongos com rinite alérgica. Além disso, a suprarregulação dos níveis de microRNA-223-3p através da administração nasal de microRNA-223-3p agomir também aumentou significativamente a concentração de IgE específica para ovalbumina, as frequências de prurido nasal e espirros, os níveis de citocinas pró-inflamatórias (IL-4, IL-5, IFN-γ) e infiltração de eosinófilos na mucosa nasal de camundongos com rinite alérgica. Além disso, o microRNA-223-3p antagomir pareceu melhorar significativamente os sintomas e a doença da mucosa nasal. Posteriormente, demonstramos pela primeira vez que o microRNA-223-3p regulou negativamente a expressão de INPP4A ao se ligar à região 3’ não traduzida 3'UTR) do gene INPP4A. Conclusões: Esses achados indicam que o microRNA-223-3p desempenha um papel importante na regulação da doença e dos sintomas da rinite alérgica, tem como alvo o gene INPP4A.

Abstract Introduction: Emerging evidence indicates that physiological and pathological conditions of the nose are posttranscriptionally regulated by microRNAs, a class of small noncoding RNAs. Recently, microRNA-223-3p has been increasingly implicated in the modulation of allergic rhinitis Objective: This study aimed to assess the role and mechanism of microRNA-223-3p in a mouse model of allergic rhinitis. Methods: The expression level of miR-223-3p was measured in the serum of 41 allergic rhinitis patients and 39 healthy controls using quantitative real time polymerase chain reaction. BALB/c mice were used to establish an allergic rhinitis model by intraperitoneal sensitization and intranasal challenge with ovalbumin. MicroRNA-223-3p agomir/antagomir was then intranasally administered to mice after ovalbumin challenge for another week. The symptoms of nasal rubbing and sneezing were recorded. Serum ovalbumin-specific immunoglobulin E concentration, microRNA-223-3p expression and proinflammatory cytokine (IL-4, IL-5, IFN-γ) levels in nasal mucosa were measured by ELISA and quantitative real time polymerase chain reaction, respectively. Histopathologic changes were evaluated using hematoxylin and eosin staining. Results: MicroRNA-223-3p levels increased significantly in both allergic rhinitis patients and allergic rhinitis mice. In addition, upregulation of microRNA-223-3p levels by nasal administration of microRNA-223-3p agomir also markedly increased the concentration of ovalbumin -specific IgE, the frequencies of nasal rubbing and sneezing, the levels of proinflammatory cytokines (IL-4, IL-5, IFN-γ) and eosinophil infiltration in the nasal mucosa of allergic rhinitis mice. Moreover, microRNA-223-3p antagomir appeared to strongly ameliorate the symptoms and pathology in nasal mucosa. Subsequently, we demonstrated for the first time that microRNA-2233p negatively regulated INPP4A expression by binding with the 3 untranslated region 3'UTR) of INPP4A. Conclusions: These findings indicate that microRNA-223-3p plays an important role in regulating the pathology and symptoms of allergic rhinitis by targeting INPP4A.

Resumo La presente revisión resume los desafíos de la aplicación, a la practica del Diseño, de las evidencias que los factores del ambiente construido contribuyen a que las personas con demencia perciban el sentido del hogar dentro de un centro de cuidado de ancianos. Diecisiete publicaciones has sido revisadas y clasificadas según fueran estudios basados en la investigación, o basados en la practica del diseño. Los estudios basados en la investigación, derivado de la epistemología científica, se enfocan en los aspectos del ambiente físico que influencian el sentido del hogar en los residentes de centro de cuidado de ancianos. Los estudios basados en la practica del diseño, desarrollan especificas estrategias basadas en evidencia de los estudios basado en la investigación. Sin embargo, en la transformación de evidencia, proveniente de la investigación, en practica de diseño, hay limitaciones debida a discordancia entre conocimiento de base y presentación de la evidencia. La investigación futura podría considerar la posibilidad de situarse en la epistemología del diseño para obtener nuevos conocimientos que inspiren la práctica del diseño mediante el enfoque de la investigación a través del diseño.

Abstract This review summarises the challenges of applying evidence that built environment factors contribute to people with dementia feeling at home in long-term care institutions. Eighteen reviewed publications are classified into research-focused and practice-focused study. Research-focused studies from scientific epistemology focus on physical environment aspects that influence residents feeling at home in the care institution. Design-focused studies develop specific design strategies based on evidence from research-focused studies. However, there are limitations in transforming research evidence into design practice due to a mismatched knowledge foundation. Future research should consider standing on design epistemology to gain new insights which reflect built environment contributions to the sense of home for people with dementia living in care institutions by Research through Design approach.

BACKGROUND: Gender has been identified as a possible influencing factor in users' information-seeking process. Previous studies have alluded to the fact that gender as a variable may be useful for a better understanding of the cognitive and social background of human information processing and may have important implications in the information-seeking process. Although a number of studies have investigated gender, amongst other variables, as having an effect on the information-seeking process of users, no attempt has been made to investigate the relationship between gender and cognitive factors on the information-seeking patterns of graduate students of Kenyatta University Library. OBJECTIVE: The study investigates gender and cognitive factors influencing the information-seeking process of graduate students at Kenyatta University Library. METHODOLOGY: To achieve this objective, the study developed a theoretical framework which can be used by academic libraries as a basis for implementing both digital and reference desk services in order to meet the dynamic user needs. The study then investigated whether there were any gender differences through the correlation coefficient in the context of expectancy theory. The motivational process amongst the male and female users was then examined to establish whether there was any difference. RESULTS: This study found no gender difference in all the variables considered, including interaction service quality, outcome (need satisfaction,) service satisfaction, users' performance of service, past experience, expectancy and effort. CONCLUSION: This study found no gender difference in all the variables investigated. The implication of the findings was that there is no need for mainstreaming gender in service programming in the library service.

Resumo Introdução: A quinase 3 sérica induzida por glicocorticoide, uma serina/treonina quinase que funciona downstream da via de sinalização PI3K, desempenha um papel crítico nos processos neoplásicos. É expressa por vários tumores e contribui para a carcinogênese. Objetivo: Investigar a expressão de quinase 3 sérica induzida por glicocorticoide no carcinoma nasofaríngeo, estudar os efeitos antitumorais do shRNA da quinase 3 sérica induzida por glicocorticoide, que inibem sua expressão em células de carcinoma nasofaríngeo, e discutir as implicações potenciais de nossos achados. Método: A expressão de proteína quinase 3 sérica induzida por glicocorticoide em linhagens de células de carcinoma nasofaríngeo (CNE-1, CNE-2, HNE-1, HONE-1 e SUNE-1) e a linhagem de células humanas imortalizadas do epitélio nasofaríngeo NP69 foram avaliadas por Western blot. A expressão da quinase 3 sérica induzida por glicocorticoide em 42 tecidos de CNF embebidos em parafina foi feita por imuno-histoquímica. Testes com MTT, citometria de fluxo e testes de raspagem foram feitos após as células CNE-2 terem sido transfectadas com o melhor plasmídeo shRNA da quinase 3 sérica induzida por glicocorticoide selecionado por Western blot, com o uso de lipofectamina para estudar seu efeito na proliferação, apoptose e migração celular. Resultados: Foi observada uma sobre-expressão da quinase 3 sérica induzida por glicocorticoide em tecidos e células de carcinoma nasofaríngeo humanas. A expressão de quinase 3 sérica induzida por glicocorticoide diminuiu acentuadamente após as células CNE-2 terem sido transfectadas com o shRNA da quinase 3 sérica induzida por glicocorticoide, conduzindo a forte inibição de proliferação e migração celular. Além disso, a taxa de apoptose aumentou nas células CNE-2 após o knockdown da quinase 3 sérica induzida por glicocorticoide. Conclusão: A expressão de quinase 3 sérica induzida por glicocorticoide foi observada com maior frequência à medida que o epitélio nasofaríngeo progride de tecido normal para carcinoma. Isso sugere que a quinase 3 sérica induzida por glicocorticoide contribui para o processo multietapas da carcinogênese do carcinoma nasofaríngeo. A quinase 3 sérica induzida por glicocorticoide representa um alvo para a terapia do carcinoma nasofaríngeo e há uma base para a investigação adicional dessa modalidade de tratamento adjuvante para o carcinoma nasofaríngeo.

Abstract Introduction: Serum- and glucocorticoid-inducible kinase 3, a serine/threonine kinase that functions downstream of the PI3K signaling pathway, plays a critical role in neoplastic processes. It is expressed by various tumors and contributes to carcinogenesis. Objective: The objective was to investigate serum- and glucocorticoid-inducible kinase 3 expression in nasopharyngeal carcinoma, to study the anti-tumor effects of serum- and glucocorticoid-inducible kinase 3 shRNA by inhibiting its expression in nasopharyngeal carcinoma cells and to discuss the potential implications of our findings. Methods: Serum- and glucocorticoid-inducible kinase 3 protein expression in nasopharyngeal carcinoma cell lines (CNE-1, CNE-2, HNE-1, HONE-1, and SUNE-1) and the human immortalized nasopharyngeal epithelium cell line NP69 were assayed by western blotting. Serum- and glucocorticoid-inducible kinase 3 expression in 42 paraffin-embedded nasopharyngeal carcinoma tissues were performed by immunohistochemistry. MTT assay, flow cytometry, and scratch tests were performed after CNE-2 cells were transfected with the best serum- and glucocorticoid-inducible kinase 3 shRNA plasmid selected by western blotting using lipofectamine to study its effect on cell proliferation, apoptosis, and migration. Results: Serum- and glucocorticoid-inducible kinase 3 was overexpressed in human nasopharyngeal carcinoma tissues and cells. Serum- and glucocorticoid-inducible kinase 3 expression decreased markedly after CNE-2 cells were transfected with the serum- and glucocorticoid-inducible kinase 3 shRNA, leading to strong inhibition of cell proliferation and migration. In addition, the apoptosis rate increased in CNE-2 cells after serum- and glucocorticoid-inducible kinase 3 knockdown. Conclusion: Serum- and glucocorticoid-inducible kinase 3 expression was more frequently observed as the nasopharyngeal epithelium progresses from normal tissue to carcinoma. This suggests that serum- and glucocorticoid-inducible kinase 3 contributes to the multistep process of NPC carcinogenesis. Serum- and glucocorticoid-inducible kinase 3 represents a target for nasopharyngeal carcinoma therapy, and a basis exists for the further investigation of this adjuvant treatment modality for nasopharyngeal carcinoma.